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1.
Chloride is required for the maximum activity of the oxygen evolving complex (OEC) while formate inhibits the function of OEC. On the basis of the measurements of oxygen evolution rates and the S2 state multiline EPR signal, an interaction between the action of chloride and formate at the donor side of PS II has been suggested. Moreover, the Fe2+Q–A EPR signals were measured to investigate a common binding site of both these anions at the PS II acceptor side. Other monovalent anions like bromide, nitrate etc. could influence the effects of formate to a small extent at the donor side of PS II, but not significantly at the acceptor side of PS II. The results presented in this paper clearly suggest a competitive binding of formate and chloride at the PS II acceptor side. 相似文献
2.
Semin BK Davletshina LN Aleksandrov AY Lanchinskaya VY Novakova AA Ivanov II 《Biochemistry. Biokhimii?a》2004,69(3):331-339
Light-induced interaction of Fe(II) cations with the donor side of Mn-depleted photosystem II (PS II(–Mn)) results in the binding of iron cations and blocking of the high-affinity (HAZ) Mn-binding site. The pH dependence of the blocking was measured using the diphenylcarbazide/2,6-dichlorophenolindophenol test. The curve of the pH dependence is bell-shaped with pK 1 = 5.8 and pK 2 = 8.0. The pH dependence of the O2-evolution mediated by PS II membranes is also bellshaped (pK 2 = 7.6). The pH dependence of the process of electron donation from exogenous donors in PS II(–Mn) was studied to determine the location of the alkaline pH sensitive site of the electron transport chain. The data of the study showed that the decrease in the iron cation binding efficiency at pH > 7.0 during blocking was determined by the donor side of the PS II(–Mn). Mössbauer spectroscopy revealed that incubation of PS II(–Mn) membranes in a buffer solution containing 57Fe(II) + 57Fe(III) was accompanied by binding only Fe(III) cations. The pH dependence of the nonspecific Fe(III) cation binding is also described by the same bell-shaped curve with pK 2 = 8.1. The treatment of the PS II(–Mn) membranes with the histidine modifier diethylpyrocarbonate resulted in an increase in the iron binding strength at alkaline pH. It is suggested that blocking efficiency at alkaline pH is determined by competition between OH– and histidine ligand for Fe(III). Because the high-affinity Mn-binding site contains no histidine residue, this fact can be regarded as evidence that histidine is located at another (other than high-affinity) Fe(III) binding site. In other words, this means that the blockage of the high-affinity Mn-binding site is determined by at least two iron cations. We assume that inactivation of oxygen-evolving complex and inhibition of photoactivation in the alkaline pH region are also determined by competition between OH– and a histidine residue involved in coordination of manganese cation outside the high-affinity site. 相似文献
3.
Direct EPR evidence of the photo-generation of superoxide radicals (O2
–.) was obtained by using a novel spin trapping probe in spinach Photosystem II (PS II) membrane fragments. The production of O2
–. was detected by following the formation of 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO) superoxide adducts (DEPMPO-OOH). The inhibition of O2
–. formation by 3-(3,4-dichlorophenyl) -1,1-dimethylurea (DCMU) and the 77 K fluorescence spectrum indicated that O2
–. were generated from PS II, not from PS I. The inhibition of O2
–. formation by DCMU also suggested that O2
–. were generated from the QBbinding site, not at a site prior to DCMU blockage. The extrinsic proteins and Mn are very important to eliminate O2
–., showing that the oxygen-evolving system is involved in O2
–. removal rather than production.This revised version was published online in October 2005 with corrections to the Cover Date. 相似文献
4.
The light-induced EPR multiline signal is studied in O2-evolving PS II membranes. The following results are reported: (1) Its amplitude is shown to oscillate with a period of 4, with respect to the number of flashes given at room temperature (maxima on the first and fifth flashes). (2) Glycerol enhances the signal intensity. This effect is shown to come from changes in relaxation properties rather than an increase in spin concentration. (3) Deactivation experiments clearly indicate an association with the S2 state of the water-oxidizing enzyme. A signal at g = 4.1 with a linewidth of 360 G is also reported and it is suggested that this arises from an intermediate donor between the S states and the reaction centre. This suggestion is based on the following observations: (1) The g = 4.1 signal is formed by illumination at 200 K and not by flash excitation at room temperature, suggesting that it arises from an intermediate unstable under physiological conditions. (2) The formation of the g = 4.1 signal at 200 K does not occur in the presence of DCMU, indicating that more than one turnover is required for its maximum formation. (3) The g = 4.1 signal decreases in the dark at 220 K probably by recombination with Q?AFe. This recombination occurs before the multiline signal decreases, indicating that the g = 4.1 species is less stable than S2. (4) At short times, the decay of the g = 4.1 signal corresponds with a slight increase in the multiline S2 signal, suggesting that the loss of the g = 4.1 signal results in the disappearance of a magnetic interaction which diminishes the multiline signal intensity. (5) Tris-washed PS II membranes illuminated at 200 K do not exhibit the signal. 相似文献
5.
Inhibition of Water Splitting Increases the Susceptibility of Photosystem II to Photoinhibition 总被引:1,自引:1,他引:0
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Photosystem II (PSII)-enriched membrane particles were isolated from peas (Pisum sativum L.) and treated in several different ways to inhibit the water oxidation reactions, but not reaction center function itself, as judged by the light-induced rate of reduction of 2,6-dichlorophenol indophenol with and without the artificial electron donor, diphenyl carbazide. It was shown that such treatments increased the susceptibility of the PSII-enriched membranes to photoinhibition. This trend was further observed if 2,6-dichlorophenol indophenol was present during the illumination with photoinhibitory light. On the other hand, protection against the enhanced photoinhibition was found when the water-splitting activity was reconstituted or when the artificial electron donor diphenyl carbazide was present during the preillumination. The results indicate that irreversible photodamage occurred within the PSII reaction center as a consequence of illumination with strong light and that the rate of this damage was enhanced under conditions that are expected to give rise to a photoaccumulation of oxidizing species such as P680+ on the donor side of PSII. This mechanism of photoinhibitory damage occurred under both aerobic and anaerobic conditions. 相似文献
6.
The effect of the paramagnetic rare earth dysprosium (Dy) onthe power saturation of EPR Signal II, was studied with thePS-II particles to obtain information on the charge distributionand structure of the donor side of PS II, which contains thethree peripheral polypeptides of 33, 24 and 18 kDa. DyCl3 andDy-EDTA complex were used as relaxing agents for Signal II,in the untreated, NaCl-washed and CaCl2-washed PS-II particles.In the untreated PS-II particles, DyCl3 significantly relievedthe power saturation of Signal IIS, whereas Dy-EDTA was lesseffective. After the NaCl washing of the PS-II particles, whichremoved the 24- and 18-kDa polypeptides, the effectiveness ofDyCl3 increased while that of Dy-EDTA did not change. In theCaCl2- washed particles, from which all the three polypeptideshad been removed, DyCl3 was slightly more effective and Dy-EDTAwas more effective than in the NaCl-washed particles. Theseresults suggest that the binding site of the 24- and /or 18-kDapolypeptides on the inner surface of PS II is negatively charged,while the binding site of the 33-kDa polypeptide is positivelycharged. (Received September 24, 1986; Accepted September 7, 1987) 相似文献
7.
磷脂酰甘油对光系统Ⅱ放氧活性的影响 总被引:1,自引:0,他引:1
The dependence of oxygen evolution in PS Ⅱ from spinach Spinacia oleracea L. on the content of exogenous anionic phosphatidylglycerol (PG) at pH 6.0 was investigated through reconstitution experiment. It was found that there was a steady increase in oxygen evolution. With increasing PG/PS Ⅱ ratio up to a maximum at concentrations ranging from 10-22 mg PG/mg chlorophyll (Chl). Then, further addition of PG resulted in the inhibitions of oxygen evolution. With a PG/PS Ⅱ ratio of 40 mg PG/mg Chl, the oxygen-evolving activity of PS Ⅱ decreased to 40% of the untreated PS Ⅱ. It is suggested that a stimulation of oxygen evolution at a low PG/Chl ratio was resulted from the structural optimization of PS Ⅱ by PG while an inhibitory effect on oxygen evolution at higher values of this ratio was ascribed to the structural changes of extrinsic proteins of PS Ⅱ owing to osmotic pressure. 相似文献
8.
The risetime of EPR signal IIvf (S IIvf) has been measured in oxygen-evolving Photosystem II particles from spinach chloroplasts at pH 6.0. The EPR signal shows an instrument-limited rise upon induction (). These data are consistent with a model where the species Z responsible for S IIvf is the immediate electron donor to P-680+ in spinach chloroplasts. A new, faster decay component of S IIvf has also been detected in these experiments. 相似文献
9.
Tiffanie Chan Yurika Shimizu Pavel Pospí?il Nobuyoshi Nijo Anna Fujiwara Yoshito Taninaka Tomomi Ishikawa Haruka Hori Daisuke Nanba Aya Imai Noriko Morita Miho Yoshioka-Nishimura Yohei Izumi Yoko Yamamoto Hideki Kobayashi Naoki Mizusawa Hajime Wada Yasusi Yamamoto 《PloS one》2012,7(12)
Environmental stresses lower the efficiency of photosynthesis and sometimes cause irreversible damage to plant functions. When spinach thylakoids and Photosystem II membranes were illuminated with excessive visible light (100–1,000 µmol photons m−1 s−1) for 10 min at either 20°C or 30°C, the optimum quantum yield of Photosystem II decreased as the light intensity and temperature increased. Reactive oxygen species and endogenous cationic radicals produced through a photochemical reaction at and/or near the reaction center have been implicated in the damage to the D1 protein. Here we present evidence that lipid peroxidation induced by the illumination is involved in the damage to the D1 protein and the subunits of the light-harvesting complex of Photosystem II. This is reasoned from the results that considerable lipid peroxidation occurred in the thylakoids in the light, and that lipoxygenase externally added in the dark induced inhibition of Photosystem II activity in the thylakoids, production of singlet oxygen, which was monitored by electron paramagnetic resonance spin trapping, and damage to the D1 protein, in parallel with lipid peroxidation. Modification of the subunits of the light-harvesting complex of Photosystem II by malondialdehyde as well as oxidation of the subunits was also observed. We suggest that mainly singlet oxygen formed through lipid peroxidation under light stress participates in damaging the Photosystem II subunits. 相似文献
10.
Photoinhibition of O2 evolution and reactions leading to millisecond-delayed light emission (ms-DLE) of chlorophyll by illumination of leaves with excess white light were investigated in wheat seedlings greened for different times in a special chamber with constant conditions (20°C; CO2 and humidity). A sharp reduction in initial and steady state rates of O2 evolution and in the intensity of different components of ms-DLE under excess light on the stage of lag-phase of chlorophyll biosynthesis (4–6h of greening) were observed. An increasing stability of the oxygen-evolving process and ms-DLE of chlorophyll during formation of the thylakoid membrane photosystems (12–24 h of greening) was shown. Rifampicin did not influence the stability of oxygen evolution whereas cycloheximide led to the intensification of photoinhibition of the initial and steady-state rates of oxygen evolution under the inhibitory light action. The early stages of photosystems formation during short time of greening of etiolated seedlings were more sensitive to the action of inhibitory light, possibly due to a weak interaction of the oxygen-evolving system components and connection with reaction centers of Photosystem II. 相似文献
11.
The effect of strong irradiance (2000 micromole photons per square meter per second) on PSII heterogeneity in intact cells of Chlamydomonas reinhardtii was investigated. Low light (LL, 15 micromole photons per square meter per second) grown C. reinhardtii are photoinhibited upon exposure to strong irradiance, and the loss of photosynthetic functioning is due to damage to PSII. Under physiological growth conditions, PSII is distributed into two pools. The large antenna size (PSIIα) centers account for about 70% of all PSII in the thylakoid membrane and are responsible for plastoquinone reduction (Qb-reducing centers). The smaller antenna (PSIIβ) account for the remainder of PSII and exist in a state not yet able to photoreduce plastoquinone (Qb-nonreducing centers). The exposure of C. reinhardtii cells to 60 minutes of strong irradiance disabled about half of the primary charge separation between P680 and pheophytin. The PSIIβ content remained the same or slightly increased during strong-irradiance treatment, whereas the photochemical activity of PSIIα decreased by 80%. Analysis of fluorescence induction transients displayed by intact cells indicated that strong irradiance led to a conversion of PSIIβ from a Qb-nonreducing to a Qb-reducing state. Parallel measurements of the rate of oxygen evolution revealed that photosynthetic electron transport was maintained at high rates, despite the loss of activity by a majority of PSIIα. The results suggest that PSIIβ in C. reinhardtii may serve as a reserve pool of PSII that augments photosynthetic electron-transport rates during exposure to strong irradiance and partially compensates for the adverse effect of photoinhibition on PSIIα. 相似文献
12.
通过探讨在水淹条件下水芹(Oenanthe javanica)叶片结构的变化以及出水对其光系统II功能和光抑制的影响,阐明水芹光合机构在水淹条件下及出水后死亡的可能原因。结果表明:水淹条件下新生沉水功能叶光系统Ⅱ(PSⅡ)最大光化学效率(Fv/Fm)、电子传递活性与对照叶片差异很小,但水淹使气生功能叶的Fv/Fm显著降低;植株总生物量呈负增长趋势;活体弱光条件下,沉水叶出水后2小时叶片相对含水量(RWC)和Fv/Fm无显著变化;中等光强和强光条件下其RWC和Fv/Fm迅速降低;离体条件下,5小时的中等光强对沉水叶的Fv/Fm影响不显著,在随后的弱光下能恢复到出水时的初始状态;强光能使沉水叶的Fv/Fm大幅降低,且弱光下不能恢复到出水时的初始水平;在解剖结构上,水芹沉水叶的叶片总厚度、上下表皮厚度和气孔大小都显著低于气生叶,而且沉水叶没有明显的栅栏组织分化,但是沉水叶上表皮的气孔密度显著高于气生叶。研究结果表明,水淹使水芹原气生叶PSⅡ功能迅速衰退,但对新生沉水叶片影响很小。水芹植株出水后,沉水叶片结构变化使其在光下保水能力下降,而强光导致了光合机构的光抑制和反应中心失活。田间条件下两者共同作用则加剧了对叶片光合机构的破坏,进而致使其死亡。 相似文献
13.
The rise time of the photoinduced, reversible EPR Signal IIvf in spinach chloroplasts is found using flash excitation to be 20 ± 10 μs. The results are interpreted as evidence that the Signal IIvf radical is an electron carrier on the donor side of Photosystem II, but probably does not result from the first donor to P680+. 相似文献
14.
水淹对水芹叶片结构和光系统II光抑制的影响 总被引:3,自引:0,他引:3
通过探讨在水淹条件下水芹(Oenanthe javanica)叶片结构的变化以及出水对其光系统II功能和光抑制的影响, 阐明水芹光合机构在水淹条件下及出水后死亡的可能原因。结果表明: 水淹条件下新生沉水功能叶光系统II(PSII)最大光化学效率(Fv/Fm) 、电子传递活性与对照叶片差异很小, 但水淹使气生功能叶的Fv/Fm显著降低; 植株总生物量呈负增长趋势; 活体弱光条件下, 沉水叶出水后2小时叶片相对含水量(RWC)和Fv/Fm无显著变化; 中等光强和强光条件下其RWC和Fv/Fm迅速降低; 离体条件下, 5小时的中等光强对沉水叶的Fv/Fm影响不显著, 在随后的弱光下能恢复到出水时的初始状态; 强光能使沉水叶的Fv/Fm大幅降低, 且弱光下不能恢复到出水时的初始水平; 在解剖结构上, 水芹沉水叶的叶片总厚度、上下表皮厚度和气孔大小都显著低于气生叶, 而且沉水叶没有明显的栅栏组织分化, 但是沉水叶上表皮的气孔密度显著高于气生叶。研究结果表明, 水淹使水芹原气生叶PSII功能迅速衰退, 但对新生沉水叶片影响很小。水芹植株出水后, 沉水叶片结构变化使其在光下保水能力下降, 而强光导致了光合机构的光抑制和反应中心失活。田间条件下两者共同作用则加剧了对叶片光合机构的破坏, 进而致使其死亡。 相似文献
15.
The effects of DCMU on the oxidizing side of PS II were studiedwith Triton-solubilized PS II membranes depleted of functionalMn. 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) non-competitivelyinhibited the diphenylcarbazide-supported (DPC-supported) photoreductionof silicomolybdate (SiMo) at concentrations more than ten timeshigher than that required for inhibition of the DPC-supportedphotoreduction of 2,6-dichlorophenolindophenol (DCIP). The maximumfluorescence intensity was also reduced by DCMU at a similarconcentration to that required for the inhibition of the SiMophotoreduction. These findings suggest two inhibitory sitesof action of DCMU in PS II: one on the reducing side and oneon the oxidizing side of PS II. The inhibition constant forDCMU in the DPC-supported SiMo-photoreduction was 10 µMin every examination. The extent of inhibition was attenuatedby modifications of the PS II oxidizing side by the presenceof functional Mn, by photoinhibition and by chemical modificationsof histidine residues and acidic amino acid residues. Our resultssuggest that DCMU binds to the PS II oxidizing side near Z,D and the high-affinity Mn-binding sites.
1 Present Address and address for all communications: NoriakiTamura (Dr.), Plant Physiology Laboratory Fukuoka Women's University,Kasumigaoka 1-1, Higashi-ku, Fukuoka, 813 Japan. FAX 092-661-2415. 相似文献
16.
The anion azide, N3
-, has been previously found to be an inhibitor of oxygen evolution by Photosystem II (PS II) of higher plants. With respect to chloride activation, azide acts primarily as a competitive inhibitor but uncompetitive inhibition also occurs [Haddy A, Hatchell JA, Kimel RA and Thomas R (1999) Biochemistry 38: 6104–6110]. In this study, the effects of azide on PS II-enriched thylakoid membranes were characterized by electron paramagnetic resonance (EPR) spectroscopy. Azide showed two distinguishable effects on the S2 state EPR signals. In the presence of chloride, which prevented competitive binding, azide suppressed the formation of the multiline and g = 4.1 signals concurrently, indicating that the normal S2 state was not reached. Signal suppression showed an azide concentration dependence that correlated with the fraction of PS II centers calculated to bind azide at the uncompetitive site, based on the previously determined inhibition constant. No evidence was found for an effect of azide on the Fe(II)QA
- signals at the concentrations used. This result is consistent with placement of the uncompetitive site on the donor side of PS II as suggested in the previous study. In chloride-depleted PS II-enriched membranes azide and fluoride showed similar effects on the S2 state EPR signals, including a notable increase and narrowing of the g = 4.1 signal. Comparable effects of other anions have been described previously and apparently take place through the chloride-competitive site. The two azide binding sites described here correlate with the results of other studies of Lewis base inhibitors.This revised version was published online in October 2005 with corrections to the Cover Date. 相似文献
17.
Transient electron paramagnetic resonance (TR EPR) at 9.8 GHz has been used to study the light-induced triplet state in single crystals of Photosystem II (PS II). The crystals were grown from a solution of PS II core complexes from the thermophilic cyanobacterium Synechococcus elongatus. The core complexes contain at least 17 subunits, including the water-oxidizing complex, and 32 chlorophyll a molecules per PS II complex. The PS II complexes are active in light-induced electron transfer and water oxidation. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with four dimers of PS II complexes per unit cell. Laser excitation was used to generate the recombination triplet state in PS II which was then studied by EPR at low temperatures (10 K). The crystal spectra show the same magnitude of the zero-field splitting (ZFS) values D, E as spectra obtained earlier for the triplet state of PS II in frozen solution. The orientation of the ZFS tensor D of the triplet state with respect to the crystallographic axes has been deduced from the analysis of angular-dependent EPR spectra. Knowledge of the orientation of the D tensor component perpendicular to the plane of the chlorophyll (D(Z)) allows an assignment on which chlorophyll of the reaction centre the triplet state is localized at low temperatures. Furthermore, the orientation of the D(X) and D(Y) components of the D tensor yielded the in-plane orientation of the respective chlorophyll in the reaction centre providing first experimental evidence for the orientation of this molecule in the PS II. 相似文献
18.
19.
以水稻品种‘II优084’为材料,测定了强光胁迫下,水稻光合速率、叶绿素荧光快速诱导曲线(OJIP)以及O2ˉ·和H2O2在水稻叶片中积累的影响。结果表明强光胁迫下,水稻的净光合速率及气孔导度下降;光系统II(PSII)反应中心关闭的比例以及电子传递链中光系统II受体侧原初醌受体(QA)的还原程度增加;PSII反应中心电子传递的量子产额、能量以及传递到下游电子链的比率下降;光抑制下PSII的过剩能量向PSI的状态装换减少;自由基的产生增加。而施加作为硫化氢(H2S)供体的外源硫氢化钠(NaHS)后,上述影响PSII活性的指标的负变化被缓解,捕光天线复合体LHC通过在两个光系统之间的移动,来调节两个光系统的能量分配。强光下H2S处理能促进LHC离开PSII,与PSI结合,从而减少PSII分配的激发能,增加PSI分配的激发能,缓解了PSII的过度还原。以上结果表明外源H2S通过促进PSII的光合活性来缓解水稻光抑制伤害。 相似文献
20.
《BBA》1985,808(1):171-179
The effects of selective removal of extrinsic proteins on donor side electron transport in oxygen-evolving PS II particles were examined by monitoring the decay time of the EPR signal from the oxidized secondary donor, Z+, and the amplitude of the multiline manganese EPR signal. Removal of the 16 and 24 kDa proteins by washing with 1 M NaCl inhibits oxygen evolution, but rapid electron transfer to Z+ still occurs as evidenced by the near absence of Signal IIf. The absence of a multiline EPR signal shows that NaCl washing induces a modification of the oxygen-evolving complex which prevents the formation of the S2 state. This modification is different from the one induced by chloride depletion of PS II particles, since in these a large multiline EPR signal is found. After removal of the 33 kDa protein with 1 M MgCl2, Signal IIf is generated after a light flash. Readdition of the 33 kDa component to the depleted membranes accelerates the reduction of Z+. Added calcium ions show a similar effect. These findings suggest that partial advancement through the oxygen-evolving cycle can occur in the absence of the 16 and 24 kDa proteins. The 33 kDa protein, on the other hand, may be necessary for such reactions to take place. 相似文献