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1.
The immune response to F antigen by a variety of inbred strains and F1 hybrids has been studied. All of the mice responding to appropriate preparations of F antigen share ak allele atH-2K orI-A. In F1 hybrids, however, this permissive gene is sometimes expressed as dominant responsiveness, while in other combinations as dominant nonresponsiveness. There appears to be a hierarchy of responsiveness among the responder strains tested. Finally, some strains produce nonprecipitating antibodies against F antigen which may represent a genetically controlled restriction of the response to this antigen. 相似文献
2.
Rolf M. Zinkernagel 《Immunogenetics》1980,10(1-4):373-382
Systemic graft-versus-host reactions (GVHR) were induced in F1 heterozygous mice by injecting 108 parental lymphocytes. The Anti-Thy 1.2-sensitive, T-cell mediated activation of macrophages was assessed by their increased capacity to destroy a facultative intracellular bacteriumListeria monocytogenes. The difference inMHC regions causing a GVHR that induced high levels of macrophage activation mapped toI-A. In contrast, differences atK orD, in any of the otherH-2 subregions or in the non-H-2 background, includingMls alone or in combination, did not induce a GVHR leading to macrophage activation, unless these differences were combined with a difference atI-A. The numbers of parental cells needed to activate macrophages via a GVHR caused byI-A vs. non-I-A differences, varied at least 30- to 100-fold. When parental cells were injected into F1 offspring of parents differing atI-J, growth ofListeria was enhanced significantly; this negative effect on macrophages was not seen when parental combinations differing atI-A alone were compared with those differing atI-A plusI-J orI-J plus otherH-2 regions. 相似文献
3.
Murine responses to immunization with 2, 4, 6-trinitrophenyl (TNP) conjugated to autogenous mouse serum albumin (MSA) in complete Freund's adjuvant (CFA) are controlled by a gene(s) in theK orI-A region of theH-2 complex. High immune responses of bothH-2
d
andH-2
b
mice have been mapped to this region of the major histocompatibility complex. No modifying effects were observed from genes to the right ofI-A in either responder haplotype. High responsiveness controlled byK
b
orI-A
b
is inherited with complete or partial recessivity, depending on the route of immunization and the sex of the responder. However, high responsiveness controlled byK
d
orI-A
d
is inherited dominantly. This unusual pattern of inheritance of immune responsiveness to TNP-MSA is consistent with the genetic mapping toK orI-A. TNP-MSA-specific T-cell reactivity following immunization with TNP-MSA in vivo was examined utilizing a T-cell-dependent proliferation assay in vitro with cells obtained from high or low responder mice. Genetic mapping and mode of inheritance in this assay for antigen-specific T-cell reactivity corresponded with results obtained from a plaque-forming cell (PFC) assay measuring antibody production by B cells. Both the proliferative and PFC responses are probably under the sameIr gene control. Both gene dosage effects and Ir-gene-product interaction could influence the generation of specific immune responsiveness in F1 hybrids between high and low responders to TNP-MSA. 相似文献
4.
The genetic control of the low response of BSVS mice to streptococcal Group A carbohydrate (GAC) was studied in crosses with responder A/J mice. F1 mice were responders. In the backcross (BSVS × A/J)F1 × BSVS mice, there were equal numbers of anti-GAC responder and nonresponder mice, indicating genetic control by a small number of major loci. The anti-GAC responses of the backcross mice showed no obligate linkage between responder status and A/JH-2 orIgC
H
alleles. However, it was observed that the average anti-GAC titers were higher in backcross mice heterozygous at these loci. The above data, a lack of low-responder F2 animals, and the segregation of a non-H-2-, non-IgC
H
-linked locus in the first and second backcross mice, indicate that the defect in the BSVS anti-GAC responsiveness involves three loci: one linked toH-2, another linked toIgC
H
, and a third locus —tentatively namedIr-GAC- not linked toH-2, IgC
H
, orHbb. 相似文献
5.
The immune response to the liver protein F antigen which, in the mouse, occurs in two allelic forms, is under sharp immunogenetic control in that only mice that possess the Ak molecule can respond to allo-F antigen. This response has been studied in a number of F1 hybrids between inbred strains and with recombinant inbred lines all of which express Ak, and which thus enable immune suppression effects to be detected. In the AKXL and AKXD sets, the hybrids with CBA are responders if H-2
k/H-2k, and usually nonresponders if H-2
k/H-2b or H-2
k/H-2d. Although this may be due to gene dosage effects, this cannot be the explanation for the low responsiveness of the H-2
k/H-2b relative to the H-2
k/H-2d mice found in CBA × BXD hybrids. For this, and other reasons, it seems likely that low responsiveness in any mouse possessing a responder A
k allele is due to suppression, and that this is mediated by the immune suppression effects of the non-H-2
k haplotype. These H-2-mediated effects can be modified, both positively and negatively, by background genes. Thus, of the ten H-2k/H-2d members of the CBA × AKXD cross, seven are low responders and three are high responders. No other typed marker has the same strain distribution pattern at present. Major unresolved questions, therefore, concern the location and mechanism of action of the background genes and the mechanism of action of the H-2 immune suppression genes. 相似文献
6.
Intraperitoneal inoculation of allogeneic lymphoid cells rapidly activates cytotoxic cells in the peritoneum which are nonadherent and express the NK-1, asialo-GM1, and Thy-1 antigens. Allogeneic spleen cells were very efficient at activating these natural killer (NK) cells, while allogeneic thymocytes were much less effective. Heat-killed allogeneic cells or sonicates also could augment NK activity. — Incompatibility atH-2K, H-2I-A, orH- 2D readily evoked NK cell activity, whileH-2S- andH-2I-E/C-associated disparities did not. Non-H- 2 differences also stimulated NK activity and augmentation was particularly evident inMls-disparate combinations. Thus, the same alloantigens which efficiently activate T cells also activate NK cells. 相似文献
7.
The immunoregulation of cytotoxic T-cell responses to the male-specific antigen H-Y in mice has been found to be genetically controlled by genes of the major histocompatibility complex (H-2). Responsiveness was mainly confined to H-2
b
strains, but it has also been found in recombinant strains, F1 hybrids, and chimeras that carry at least part of the H-2
b
haplotype. By using a different immunization procedure it has been shown recently that an H-2
k
mouse strain (CBA) is also able to mount an equivalent H-Y-specific response. We investigate here, by applying this immunization technique, the responsiveness of other H-2
k
strains and of strains of other independent H-2 haplotypes. Both responders and nonresponders are found in three haplotypes: k, s, and d. The strain distribution pattern of responsiveness shows a combined influence of non-H-2 and H-2 genes. In certain strains there is a high variability in responsiveness between genetically indentical individual animals. We discuss a model of immune response (Ir) gene function which could account for these observations. 相似文献
8.
The genetic control of the immune response to H-4 histocompatibility alloantigens is described. The rejection of H-4.2-incompatible skin grafts is regulated by anH-2-linkedIr gene. Fast responsiveness is determined by a dominant allele at theIrH-4.2 locus. TheH-2
b
,H-2
d
, andH-2
s
haplotypes share the fast response allele;H-2
a
has the slow response allele. Through the use of intra-H-2 recombinants, we have mapped theIrH-4.2 locus to theI-B subregion of theH-2 complex; theH-2
h4
,H-2
15, andH-2
t4
haplotypes are fast responder haplotypes. These observations suggest that the strength of non-H-2 histocompatibility antigens is ultimately determined by the antigen-specific recipient responsiveness. 相似文献
9.
Mauro S. Sandrin Glen H. Tobias Ian F. C. McKenzie Gunter J. Hämmerling 《Immunogenetics》1981,14(6):507-516
The Ia.8 and 9 specificities detected either by conventional or monoclonal antisera (Ia.m3, 4) are present in strains bearing the b H-2 haplotype, but absent from those with the k haplotype. It would be expected that the (b x k)F1 hybrids would have approximately half the amount of these specificities found on the b parent, but the Ia.8 and 9 specificities are absent or reduced in this F1 hybrid, though not on F1 LPS blasts. Examination of appropriate H-2 congenic strains demonstrated that only the k haplotype confers the absence of these specificities on H-2
b
— it was not observed with b, d, q, r or s haplotypes. In the k haplotype the gene(s) responsible for this effect is mapped to the I-A
k
subregion. The reason for this low expression effect is not clear but the observation has important implications for the relationship of Ia specificities and Ir genes and may serve to explain the low responder status of certain F1 hybrids, e. g., to TNP-mouse serum albumin, as observed elsewhere. 相似文献
10.
Charles A. Janeway Jr. William E. Paul Theodore P. Werblin Rose Lieberman 《Immunogenetics》1976,3(1):393-400
Cooperative interactions between T and B cells from the congenic inbred mouse strains B10.A(2R) and B10.A(4R) in antibody responses controlled byIr genes have been studied. Within theI region of the MHC, these strains share only theI-A subregion. TheIr gene controlling responsiveness to IgA maps in theI-A subregion, both strains being responders to IgA. T cells from 2R mice collaborate effectively with B cells from 2R or 4R mice for antihapten antibody responses to DNP-IgA. TheIr gene controlling responses to IgG maps in theI-B subregion, and 2R mice are nonresponders for this antigen. Nevertheless, 2R T cells primed with IgG can help responder (4R) B cells -but not syngeneic nonresponder (2R) B cells -in responding to DNP-IgG. These results indicate that mice lacking theIr-IgG gene nonetheless may develop helper T lymphocytes specific for myeloma proteins. In addition, they indicate that cells from congenic mice sharing only theA subregion of theI region can collaborate efficiently. 相似文献
11.
The level of cell proliferation and interleukin-2 (IL-2) production observed in an anti-Mls mixed lymphocyte reaction between spleen cells from H-2 compatible, Mls incompatible mouse strains is determined by the H-2 haplotype of the mouse combination. Thus, while AKR (H-2
k) spleen cells stimulated strong M1sa responses in H-2k responder cells, AKR H-2b spleen cells stimulated no or negligible M1sa responses in responder cells from H-2
bmouse strains. This effect was observed at the levels of IL-2 production and cell proliferation. The magnitude of the response observed using F1 (H-2
k/H-2
b) responder cells was found to be a function of stimulator rather than responder cells. The poor stimulatory capacity of AKRH-2
bspleen cells was also shown not to be due to the loss of the stimulatory Mls
aallele during the construction of the congenic strain from AKR and C57BL/6 parental strains. Using stimulator cells from a second series of congenic mice, we found H-2
b(strain DLLP) again to represent a poorly Mlsa stimulatory H-2 haplotype. In addition, H-2q (DBA/1) cells displayed very poor Mlsa stimulatory potential while H-2d (D1.C) cells were efficient Mlsa stimulators. Again the effect was shown to be at the level of the stimulator cells. In toto, our findings indicate that the H-2
kand H-2
dhaplotypes encode strong Mlsa stimulatory potential while the H-2
band H-2
qhaplotypes determine poor Mlsa stimulatory potential in primary in vitro responses, measured as cell proliferation and IL-2 production.Abbreviations used in this paper: CTL
cytotoxic T lymphocyte
- IL-1
interleukin-1
- IL-2
interleukin-2
- MLR
mixed lymphocyte reaction
- NMS
normal mouse serum 相似文献
12.
The T-cell mediated immune responses to the male specific minor histocompatibility antigen H-Y in mice have been studied extensively as a model for immune responses to other weak antigens like tumor antigens or autoantigens. In a recent analysis of the strain distribution of the cytotoxic T-cell (Tc-cell) responsiveness to H-Y, it has been found that genes both within and outside the H-2 complex exert an interactive control. Whereas the H-2
b
strains all are high responders, independent of their non-H-2 background, other H-2 haplotypes (d, k, and s) only allow for a response if they are combined with certain non-H-2 genes. The H-2-linked immune response genes (Ir-genes) have been previously mapped to the I and K or D region of the H-2 complex, but the mapping of the non-H-2 genes has not yet been established. In this study evidence is presented, using recombinant inbred strains and immunoglobulin heavy chain (Igh) congenic strains of mice, to show that there is more than one non-H-2 Ir-gene involved, that the main controlling genes are not linked to the Igh complex, and that at least one non-H-2 Ir-gene is linked to the H-3 region on chromosome 2. This region includes genes for beta-2-microglobulin (2m), the Ly-mllalloantigen a polymorphic cell surface glycoprotein (Pgp-1), a B-cell specific antigen Ly-4, a transplantation antigen H-3, and genes (Ir-2) controlling the immune response to Ea-1 and H-13. 相似文献
13.
The antibody response against the H-2.2 specificity has been studied in three H-2
d
strains, B10.D2, DBA/2, and BALB/c, and their hybrids (B10.D2 × DBA/2)F1 and (B10.D2 × BALB/c)F1. The genetic control of the response appears to be complex: The three pure strains are responders, whereas both hybrids when immunized with C3H-HTG are nonresponders. Individual analysis of N3 offspring is compatible with the idea that, in this combination, an Ea-4 incompatibility between donor and immunized strain is necessary for the anti-H-2.2 response to occur. H-2
d
/H-2
k
hybrids (B10.BR × B10.D2)F1 or (B10.BR × DBA/2)F1 are responders when immunized with C57BL/10 (H-2
b
) but not with B10.A(2R) (H-2
h
), indicating that simultaneously recognized H-2 specificities are necessary for the anti-H-2.2 response. 相似文献
14.
We examined multiple genetically regulated Immoral and cell-mediated immune (CMI) responses to poly(glu60ala30tyr10) (GAT) using a panel of mouse strains. We show that assignment of responder/nonresponder status depends upon the assay method. In addition, two distinct categories of nonresponder mice were found: (1) those which are unresponsive by all parameters tested (H-2
q
and H-2
s
haplotypes) and (2) those which are partially nonresponsive [H-2
bm12 mutant strain—a low/nonresponder by splenic plaque-forming cell (PFC) and delayed-type hypersensitivity (DTH) responses, but exhibits B6 parental levels of high GAT-specific T-cell proliferation (Tprlf) and interleukin-2 production]. The distinction between these two nonresponder types was confirmed by complementation tests in which significant GAT-specific PFC and DTH responses were seen in (H-2
q
× H-2
bm12)F1 hybrids, but not in (H-2
q
× H-2
s
)F1 hybrids. Suppressor T cells (Ts) also play a selective role in nonresponsiveness to GAT. Cyclophosphamide treatment of nonresponders (to eliminate Ts activity) as well as immunization with GAT coupled to the immunogenic carrier MBSA result in the development of GAT-specific humoral, but not CMI responses. Our results indicate that the T cell is the cellular site of Ir gene expression and that Tprlf responses do not correlate with functional helper T-cell activity and suggest distinct, multi-step Th/Ts regulatory pathways in the development of humoral and CMI effector functions. 相似文献
15.
We previously noted that Mlsa,c C58/J responder cells proliferated unexpectedly to H-2k-compatible Mlsa or Mlsc prototypic stimulator cells in a primary mixed lymphocyte reaction. The present investigation was performed to evaluate whether the response of C58/J T cells to these H-2- and Mls-compatible stimulator cells could functionally identify a newly-defined member of the Mls superantigen family through its allostimulatory ability. We observed that C58/J responder cells also proliferated when cultured with H-2-compatible prototypic Mlsnull, Mlsb (nonstimulatory), or Mlsa,c splenic stimulator cells. The widely distributed nature of the non-MHC ligand recognized by C58/J T cells is indicated by the finding that 11 of 12 H-2k inbred mouse strains clearly expressed this specificity. A gradient of stimulatory capacity from low to high across this newly-defined non-MHC difference was detected with splenocytes from these different inbred mouse strains. The Mlsa,c genetic composition of C58/J was confirmed by the observation that crossing C58/J with parental B10.BR (responsive to both Mlsa and Mlsc determinants) generated F1 progeny that were unresponsive to H-2k-compatible Mlsa, Mlsc, or Mlsa,c stimulator cells. Like prototypic Mlsa and Mlsc, the non-MHC specificity recognized by C58/J responder cells, termed Mlsf, was particularly sensitive to radiation (versus mitomycin C) treatment of the stimulator cells, was greatly augmented after anti-IgD activation of splenic stimulator cells, was blocked with anti-MHC class II antibody, and was effectively presented by phenotypically normal female but not B cell-defective xid+ male CBA/N F1 stimulator cells.
Address correspondence and offprint requests to: J. J. Ryan 相似文献
16.
Mice of the H-2b haplotype responded to the sequential polymer poly(Tyr-Glu-Ala-Gly) in the in vitro T-cell proliferative assay, irrespective of whether they were homozygous or heterozygous at the H-2b locus. The antibody responses of the H-2b congenic mice to this polymer were variable, with A.BY and BALB.B showing responses better than those of C57BL/6 and C57BL/10 strains. The antibody responses of the F1 progeny of (responder × nonresponder) strains of mice to this polymer are generally lower than the responder parents. F1 mice with C57BL/10 background were the poorest responders. Studies with F2 mice and backcross progenies of selective breeding of high and low antibody responder (C57BL/6 × BALB/c) F1 to high responder C57BL/6 mice indicated that both non-H-2 genes and H-2 gene dosage effects influenced the magnitude of the humoral antibody responses. Animals having low responder non-H-2 background and only half the dosage of the responder immune response genes has greatly diminished antibody responses. 相似文献
17.
Production of indirect TNP-specific plaque-forming cells (PFC) in response to immunization with 2, 4, 6-trinitrophenyl (TNP) conjugated to autogenous mouse serum albumin (MSA) in complete Freund's adjuvant (CFA) is underH-2 control. On the C57BL/10 (B10) background,H-2
b andH-2
d
strains of mice are high responders, whereasH-2
a
,H-2
k
orH-2
y2
strains yield low levels of indirect TNP-specific PFC. An unusual pattern of inheritance has been revealed in B10 congenic mice: high responsiveness controlled byH-2
b
is inherited recessively, while high responsiveness controlled byH-2
d
is inherited dominantly. On the C3H and A strain backgrounds, high responsiveness controlled byH-2
b
is partially recessive;H-2
b
/H-2
a
F1 mice respond with 20%-40% of the high responderH-2
b
response. Yet, high responsiveness controlled by theH-2
d
haplotype remains dominant on the C3H background. A hierarchy of haplotypes in order of decreasing immune responsiveness to TNP-MSA is evident as follows:H-2
d
>H-2
b
>H-2
k
,H-2
a
orH-2
y2
. The unusual patterns of inheritance in the TNP-MSA system reveal graded regulation of responsiveness attributable to bothH-2 and non-H-2 genes. 相似文献
18.
A secondary in vitro response to alphaviruses Bebaru, Sindbis, and Semliki Forest is described. Optimum response appears at day 5–6 of culture. The cells responsible for lytic activity are nonadherent, -positive, Ig–, and mainly Ly-2.1 positive. Out of five haplotypes tested (H- 2
d
,H- 2
b
,H- 2
s
,H- 2
q
, andH- 2
k
) onlyH- 2
k
was a responder. Genetic mapping of the response located it solely in theD region of theH- 2 complex. The other four haplotypes responded with a high antiself activity after a second stimulation with viruses. This antiself response also maps in theD region of theH- 2 complex. No complementation was observed in F1 hybrids between responder and nonresponder strains. 相似文献
19.
Alloantibodies specific for non-H-2 histocompatibility antigens of the mouse have been produced. Immunization (BALB/cJ×DBA/2J)F1 anti-B10.D2/n was conducted, followed by hemagglutination, immunofluorescence, and mixed hemabsorption tests on absorbed and unabsorbed sera. The results indicate that antibodies specific for H-3a and H-8a antigens are present. In addition, H-8a antigenic determinants were detected on erythrocyte membrane surfaces, as well as on cells of other body tissues. 相似文献
20.
The immune response of T lymphocytes to avidin was measured by proliferative assays, antibody production and delayed-type hypersensitivity. Mice ofH-2
k haplotypes were found to be low responders, whereas mice of other haplotypes, and particularly ofH-2
s
, were high responders.Ir genes controlling this response were mapped to theI subregion ofH-2. Helper T cells were found to be responsible for the Ir phenotype of antibody production. These results indicate the feasibility of using the avidin-biotin complex as a tool for studying molecular mechanisms by which antigens underIr gene control are processed and presented to T lymphocytes.Abbreviations used in this paper
Ir
genes, immune-response genes
-
H-2
murine major histocompatibility complex
- APC
antigen-presenting cell
- OA
ovalbumin
- BSA
bovine serum albumin
- DNP
dinitrophenyl
- DNP-OA
DNP-ovalbumin
- DNP-Av
DNP-avidin
- DNP-BSA
DNP-bovine serum albumin
- CFA
complete Freund's adjuvant
- PPD
purified protein derivative
- PBS
phosphatebuffered saline
- IP
intraperitoneal
- LNC
lymph-node cells
- DTH
delayed-type hypersensitivity 相似文献