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1.
K. T. Bird T. C. Chiles R. E. Longley A. F. Kendrick M. D. Kinkema 《Journal of applied phycology》1993,5(2):213-218
Protein extracts from 22 species of marine macroalgae from Florida and North Carolina were compared for their abilities to
agglutinate sheep and rabbit erythrocytes. Protein extracts from 21 algal species agglutinated rabbit erythrocytes compared
to 19 for sheep erythrocytes. However, agglutination by brown algal extracts was variable. The agglutination produced by protein
extracts from Dictyota dichotoma could be blocked by addition of polyvinylpyrrolidone. Protein extracts from North Carolina macroalgae were also tested against
five bacterial species. Three of these agglutinated bacterial cells. Ulva curvata and Bryopsis plumosa agglutinated all five species. Protein extracts from five species of Florida algae were tested for their effects on mitogenesis
in mouse splenocytes and human lymphocytes. Gracilaria tikvahiae HBOI Strain G-5, Ulva rigida and Gracilaria verrucosa HBOI Strain G-16S stimulated mitogenesis in mouse splenocytes, while Gracilaria tikvahiae HBOI Strain G-16stimulated mitogenesis in human lymphocytes. 相似文献
2.
A. L. P. Freitas D. I. A. Teixeira F. H. F. Costa W. R. L. Farias A. S. C. Lobato A. H. Sampaio N. M. B. Benevides 《Journal of applied phycology》1997,9(6):495-501
Aqueous protein extracts from 30 Brazilian marine algae were examined for haemagglutinating activity using native and enzyme-treated
rabbit, chicken, sheep and human erythrocytes. Most extracts agglutinated at least one of the blood cells used. Sheep and
rabbit erythrocytes were more suitable for detection of the agglutinating activity. The minimum protein concentration necessary
to produce positive agglutination was usually lower with enzyme-treated erythrocytes than native ones. The five algal protein
extracts showing the greatest haemagglutination titre were tested for sugar-binding specificity. Only the activity present
in the green alga Cauler pacupressoides was inhibited by simple sugars and not by the glycoproteins tested. The activity of
the other four extracts was inhibited by at least one of the glycoproteins utilised.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
3.
坛紫菜凝集素的糖结合专一性和细胞凝集作用 总被引:4,自引:0,他引:4
坛紫菜的磷酸盐缓冲液浸取液,经硫酸铵沉淀和DEAE-Sepharose,SephadexG-100二步层析纯化,获得纯化的坛紫菜凝集素(PHL)。该凝集素能与3种单糖(阿拉伯糖,半乳糖,木糖)及麦芽糖专一性结合,其中与麦芽糖结合最强。细胞凝集实验结果显示,PHL能凝集兔,绵羊及鸡红细胞而不能凝集鸭,鸽子及人血红细胞,PHL还能凝集海洋微藻-绿色巴夫藻和淡水微藻-蛋白核小球藻,它们的凝集活性与藻细胞密度有关。不同状态的细菌和酵母细胞对PHL反应不同,表明随着细胞状态的改变,细胞表面的凝集素受体也随之发生变化。 相似文献
4.
A potent agglutinin of rabbit and sheep red blood cells, obtained from the red alga Gracilaria tikvahiae, was purified by ammonium sulfate fractionation, ion exchange, gel filtration, and hydroxylapatite chromatography. Human A and B blood group erythrocytes were also agglutinated, whereas human O blood group erythrocytes were not agglutinated. The hemagglutination titer was not significantly affected by the addition of EDTA or the divalent cations Ca2+, Mg2+, or Mn2+. The carbohydrate specificity was characterized by hemagglutination inhibition using various monosaccharides, glycoproteins, and glycopeptides. The results suggested that the agglutinin has affinity for N-acetylneuraminic acid as well as glycoconjugates containing N-acetylneuraminic acid. 相似文献
5.
Ethanol extracts of 12 marine unicellular algae were assayed for agglutinating activity against native and enzyme-treated
human and animal erythrocytes. All of the algae assayed agglutinated at least one group of normal erythrocytes from humans.
Notably, all algal extracts agglutinated erythrocytes of hemophilia patients arising from coagulation disorders. Meanwhile,
all algae had a strong reaction with monkey erythrocytes, but to a lesser extent or not at all with sheep erythrocytes. Both
trypsin and pronase improved the detection of most algal agglutinins and caused a drastic increase in hemagglutinating activity
after treatment for 2 h or more. However, hemagglutinating activity decreased or disappeared completely when two extracts
of different algal species were combined. Journal of Industrial Microbiology & Biotechnology (2000) 24, 262–266.
Received 24 September 1999/ Accepted in revised form 06 January 2000 相似文献
6.
Agglutinating activity often varies both between and within the algal species assayed. However, it is difficulty to interpret
such variation without further analysis. We report a statistical analysis of agglutinating activities against human, cow,
sheep, and pig erythrocytes, using cell extracts from 43 taxa (strains) of freshwater microalgae. Most of the extracts agglutinated
erythrocytes from at least one of the sources, but pig erythrocytes appeared to be most suitable for the detection of agglutination
reactions. Chlorella cell extracts preferentially agglutinated human erythrocytes, whereas extracts of other taxa were less active against mammalian
erythrocytes. Cluster analysis generated four distinct subclusters of taxa, characterized by different specificities for antigens
or carbohydrate receptors on the erythrocytes. Principal component analysis further separated the agglutination characteristics
of Chlamydomonas from Chlorella on the first two components. Specificity for pig erythrocytes accounted for most of the clustering or grouping of algal taxa
in multivariate analysis. However, clustering or grouping patterns of Chlorella species on haemagglutinating activity resembled that based on DNA sequences, revealing a possible genetic connection of agglutinins
and their biochemical characteristics in algal cells. Variability of agglutination reactions among the algae investigated
is simplified and interpreted most easily using multivariate analysis. 相似文献
7.
Abstract The hemagglutinating activity of Clostridium perfringens enterotoxin (CPE) was studied after trypsin treatment. Untreated CPE did not show any hemagglutinating activity to human type A, B, and O, sheep, chicken, horse, guinea-pig, or rabbit erythrocytes. Trypsinized CPE resulted in a more than 100-fold increase in hemagglutinating activity with rabbit erythrocytes only. Other erythrocytes and trypsinized rabbit erythrocytes were not agglutinated at all. The hemagglutinating activity of CPE was also found on treatment with a lysine-specific proteinase. On the other hand, trypsinized CPE did not significantly increase the cytotoxic and enterotoxic activities. The binding reaction between trypsinized and rabbit erythrocytes was not inhibited by any mono-, di-, or polysaccharides, glycoproteins or ganglioside mixtures. These results suggest that the hydrolysis of bonds involving lysine residues is mainly required for hemagglutinating activity, and that the receptor for trypsinized CPE on rabbit erythrocytes is probably the protein moiety. 相似文献
8.
C.Y. Chu W.R. Liao R. Huang L.P. Lin 《World journal of microbiology & biotechnology》2004,20(8):817-825
We analysed the haemagglutinating activity of algal extracts from 44 species of freshwater microalgae against native and trypsin/papain-treated cow, pig, sheep, and human A-, B-, and O-type erythrocytes. Algal extracts obtained with aqueous ethanol exhibited higher haemagglutinating activity than those obtained with aqueous acetone. Most of the algal extracts agglutinated at least one of the erythrocyte types analysed. Human erythrocytes were the most sensitive of the cell types analysed. In the other species, the sensitivity of algal haemagglutinating activity for erythrocytes was pig > sheep > cow. Pre-treating erythrocytes with trypsin and papain improved the detection of most algal agglutinins and increased the haemagglutination titre; pre-treatment with papain was most effective for pig erythrocytes. Algal extracts stored at –20 °C for 4 months lost their haemagglutinating activity. Algal extracts also exhibited strong antibiotic activity against food pathogenic bacteria, especially against Bacillus. Our numerical taxonomy data showed that these microalgae might be grouped into several clusters according to their haemagglutinating activity. The detection of haemagglutinating activity may provide an efficient biochemical or physiological character to classify and differentiate microalgae. Our results suggest that freshwater microalgae might provide a potent source of haemagglutinins and antibacterial compounds for biochemical and medical studies and applications. 相似文献
9.
Binding of rabbit hemorrhagic disease virus to antigens of the ABH histo-blood group family 总被引:6,自引:0,他引:6
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Ruvoën-Clouet N Ganière JP André-Fontaine G Blanchard D Le Pendu J 《Journal of virology》2000,74(24):11950-11954
The ability of rabbit hemorrhagic disease virus to agglutinate human erythrocytes and to attach to rabbit epithelial cells of the upper respiratory and digestive tracts was shown to depend on the presence of ABH blood group antigens. Indeed, agglutination was inhibited by saliva from secretor individuals but not from nonsecretors, the latter being devoid of H antigen. In addition, erythrocytes of the rare Bombay phenotype, which completely lack ABH antigens, were not agglutinated. Native viral particles from extracts of infected rabbit liver as well as virus-like particles from the recombinant virus capsid protein specifically bound to synthetic A and H type 2 blood group oligosaccharides. Both types of particles could attach to adult rabbit epithelial cells of the upper respiratory and digestive tracts. This binding paralleled that of anti-H type 2 blood group reagents and was inhibited by the H type 2-specific lectin UEA-I and polyacrylamide-conjugated H type 2 trisaccharide. Young rabbit tissues were almost devoid of A and H type 2 antigens, and only very weak binding of virus particles could be obtained on these tissues. 相似文献
10.
《Phytochemistry》1987,26(5):1335-1338
An agglutinin has been isolated from the marine red alga Carpopeltis flabellata by ammonium sulphate fractionation, affinity chromatography on a yeast mannan-Sepharose 4B column and gel permeation HPLC. This new protein, designated carnin, is a monomeric glycoprotein with a Mr of 25 000, and it contains large amounts of Gly and Asx. It strongly agglutinated untreated rabbit, mouse and horse erythrocytes, and very weakly untreated human erythrocytes, whereas it did not agglutinate untreated sheep and chicken erythrocytes. Treatment of the erythrocytes with trypsin affected their sensitivity to haemagglutination by the agglutinin. The haemagglutinating activity was inhibited only by glycoproteins with N-glycosidic sugar chains. The activity was not affected by divalent cations. Carnin also showed mitogenic activity for T lymphocytes from mouse spleen. It inhibited the normal embryonic development of marine invertebrates. 相似文献
11.
We have isolated by affinity chromatography a lectin from the seeds of the winged bean (Psophocrapus tetragonolobus) which agglutinated human (group A, B and O), sheep and rabbit, but not mouse erythrocytes. A molecular weight of 41,000 was obtained from gel filtration, and on sodium dodecyl sulphate polyacrylamide gel electrophoresis a single polypeptide chain of molecular weight 35,000 was seen both before and after reduction. Isoelectric focussing of the lectin on polyacrylamide gel gave a single band with a calculated isoelectric point of 4.0. The lectin was found to be rich in acidic amino acids; cysteine was not detected. Carbohydrate analysis revealed no covalently bound sugars.Abbreviations PBS
phosphate-buffered saline
- SDS
sodium dodecyl sulphate
- PAGE
polyacrylamide gel electrophoresis
- WBL
winged bean lectin
- HPLC
high performance liquid chromatography 相似文献
12.
Takashi Hamasaki Kuniaki Matsui Kunihiro Isono Yuichi Hatsuda 《Bioscience, biotechnology, and biochemistry》2013,77(7):1769-1770
Neurospora sitophila produced extracellular and cell wall-associated lectins. The addition of l-sorbose to a culture resulted in a decrease in the production of the former lectin and complete abolition of the latter. The lectin in the culture filtrate was purified by bovine submaxillary mucin-conjugated Sepharose chromatography. The molecular weight of the lectin was calculated to be approx. 40,000 by Sephacryl S-200 gel filtration, and that of the subunit to be approx. 22,000 by SDS/polyacrylamide- gel electrophoresis. The lectin was not inhibited by simple sugars or their homopolymers. It was inhibited strongly by glycoproteins from human erythrocyte membrane and bovine submaxillary mucin, and moderately by α1-acid glycoprotein from human plasma, human IgA and IgM, and fetal calf fetuin. The lectin agglutinated human type A, B and O erythrocytes to the same degree. Erythrocytes from chick, horse, rabbit and sheep were more efficiently agglutinated. 相似文献
13.
A haemagglutinating material was isolated and purified from the phosphate buffered saline extract of the seeds of the jack
fruit using an immobilized N-aeetyl-D-galactosamine column. This material was composed of two iso-lectins of molecular masses
11 500 and 15 000. The lectins agglutinated native washed red blood cells of the human A, B and 0 groups and sheep, rabbit
and mouse erythrocytes. The lectins were found to be composed of single polypeptide chains and they contained no covalently
linked sugars. The lower molecular mass material was present in considerably greater quantity than the higher molecular mass
component. On isoelectric focussing on PAG the lectins gave a spread of components with calculated pI between 6.0 and 8.3. 相似文献
14.
Jinzhou Huang Cyril J. Smyth Nicholas P. Kennedy John P. Arbuthnott P.W.Napoleon Keeling 《FEMS microbiology letters》1988,56(1):109-112
Abstract Thirty-one isolates of Campylobacter pylori , screened for their ability to agglutinate a panel of erythrocyte species, could be divided into two phenotypic groups on the basis of their ability to agglutinate human A and O erythrocytes, a property which correlated strongly with their ability to agglutinate horse and cat erythrocytes. Isolates which agglutinated human red blood cells exhibited a broad-spectrum haemagglutination profile on other red blood cells including dog, goat, guinea-pig, ox, rat and sheep erythrocytes. Agglutination dog, guinea-pig, horse and human erythrocytes by C. pylori was mannose-resistant. Haemagglutination was not inhibited by other saccharides tested nor by two glycoproteins or serine. The bacterial ligand was protease- and heat-sensitive. Neither protease nor neuraminidase treatment of erythrocytes prevented agglutination. 相似文献
15.
Hot and cold aqueous extracts were prepared from 22 commonly ingested fruits, vegetables, and seeds. When tested by agar diffusion, extracts from 13 and 10 of the foods formed precipitin bands with samples of normal rabbit serum and human saliva, respectively; extracts from four of the foods also reacted with antigen extracts of strains of Streptococcus mutans. When added to rabbit antiserum, extracts from 18 of 21 foods tested inhibited reactivity with antigen extracts derived from S. mutans MT3. Extracts from 16 foods agglutinated whole S. mutans cells, whereas those from 10 foods agglutinated human erythrocytes of blood types A and B. The lectin-like activities of extracts which reacted with human saliva were studied further. Pretreatment of saliva-coated hydroxyapatite (S-HA) beads with extracts of bananas, coconuts, carrots, alfalfa, and sunflower seeds markedly reduced the subsequent adsorption of S. mutans MT3. Pretreatment of S-HA with banana extract also strongly inhibited adsorption of S. mutans H12 and S. sanguis C1, but it had little effect on attachment of Actinomyces naeslundii L13 or A. viscosus LY7. Absorption experiments indicated that the component(s) in banana extract responsible for inhibiting streptococcal adsorption to S-HA was identical to that which bound to human erythrocytes. The banana hemagglutinin exhibited highest activity between pH 7 and 8, and it was inhibited by high concentrations of glucosamine, galactosamine, and, to a lesser extent, mannosamine. Other sugars tested had no effect. The selective bacterial adsorption-inhibiting effect noted for banana extract was also observed in studies with purified lectins. Thus, pretreating S-HA with wheat germ agglutinin and concanavalin A inhibited adsorption of S. mutans MT3 cells, whereas peanut agglutinin, Ulex agglutinin, Dolichos agglutinin, and soybean agglutinin had little effect; none of these lectins affected attachment of A. viscosus LY7. Collectively, the observations suggest that many foods contain lectins which can interact with components of human saliva and S. mutans cells. Because of their potential to influence host-parasite interactions in the mouth and elsewhere in the gastrointestinal canal, these reactions warrant further study. 相似文献
16.
《Bioscience, biotechnology, and biochemistry》2013,77(10):1736-1739
Two Ca2 +–dependent lectins were purified from the sea cucumber Stichopus japonicus by affinity chromatography on lactosyl-Sepharose 4B and ion-exchange chromatography on Q-Sepharose. Their molecular masses were estimated to be 13kDa (SJL-I) and 15kDa (SJL-II) on SDS-PAGE. SJL-I agglutinated rabbit erythrocytes as well as human A, B, and O-type erythrocytes, but SJL-II agglutinated only rabbit erythrocytes. Hemagglutination by SJL-I was competitively inhibited by N-acetyl-D- galactosamine and galactose-containing carbohydrates. On the other hand, only lactose, melibiose, and raffinose gave weak inhibition of hemagglutination by SJL-II, suggesting that SJL-II may have high specificity for particular complex carbohydrate(s) on the surface of rabbit erythrocytes. SJL-II was activated at ten times lower Ca2+ concentration than SJL-I. Both lectins lost activity in acidic pH, while SJL-I appeared more stable down to pH 4.5. 相似文献
17.
Le Dinh Hung Bui Minh Ly Vo Thi Dieu Trang Ngo Thi Duy Ngoc Le Thi Hoa Phan Thi Hoai Trinh 《Journal of applied phycology》2012,24(2):227-235
Aqueous extracts from 42 species of Vietnamese marine macroalgae, including 17 Chlorophyta, 22 Rhodophyta, and three Phaeophyta
species, were examined for hemagglutination activity using native and enzyme-treated different animal and human erythrocytes.
All extracts agglutinated at least one type of erythrocytes tested. Strong activity was detected in extracts from four Chlorophyta
(Caulerpa serulata var. boryana, Caulerpa sertularioides f. longipes, Halimeda velasquezii, and Halimeda discoidea) and two Rhodophyta species (Gelidiella acerosa and Titanophora pulchra) with enzyme-treated rabbit and horse erythrocytes. The hemagglutinins of some active species were examined for sugar-binding
specificity, pH, temperature stability, and divalent cation independency using ammonium sulfate precipitates prepared from
their extracts. In a hemagglutination–inhibition test with various monosaccharides and glycoproteins, none of the hemagglutinins
had affinity for monosaccharides. The activity of the hemagglutinins was inhibited by some glycoproteins tested. The inhibition
profiles with glycoproteins were different depending on hemagglutinin species, suggesting the presence of lectins specific
for complex N-glycans, high mannose N-glycans or O-glycans. On the other hand, the activities of almost all algal hemagglutinins
were stable over a wide range of pH and temperature, and independent of the presence of divalent cations, except Gelidiopsis scoparia hemagglutinin, its activity was dependent on the presence of divalent cations. These results suggest that Vietnamese marine
macroalgae may be good sources of useful lectins for many biological applications. 相似文献
18.
Singh BR Singh VP Agarwal M Sharma G Chandra M 《Indian journal of experimental biology》2004,42(3):303-313
Haemolysin patterns of 175 strains of different Salmonella enterica subspecies enterica serovars isolated from different animal sources and places were determined using 11 different blood agar media made with either non-washed horse/sheep erythrocytes or with washed erythrocytes of cattle, sheep, horse, goat, rabbit, guinea pig, and human A, O and B blood groups. Study on 47 strains belonging to 10 serovars of Salmonella from buffalo meat (buffen), 42 strains of 11 serovars from goat meat (chevon): 16 strains of Salmonella enterica serovar Paratyphi B and 25 of S. enterica serovar Paratyphi B var Java from fish, meat, meat products and clinical cases; 45 isolates of S. Abortusequi from aborted mares (18), fetal contents (21), aborted donkey mares (2) and 4 reference strains, revealed that all host restricted Salmonella namely, S. enterica serovar Gallinarum, S. enterica serovar Anatum, S. enterica serovar Abortusequi and S. enterica serovar Paratyphi B could be divided into different haemolysin types based on their inability to produce haemolysis on one or more types of blood agar, while strains of all zoonotic Salmonella serovars induced haemolysis on all the 9 types of blood agar made of washed erythrocytes. None of 175 Salmonella could produce hemolytic colonies on blood agar made of non-washed horse/ sheep erythrocytes. Haemolysin type I (lysing all types of washed erythrocytes) was the commonest one among all serovars except S. Abortusequi, none of which lysed horse erythrocytes. Salmonella enterica serovar Abortusequi having hemolytic activity against sheep erythrocytes were more invasive but had lesser ability to survive in sheep mononuclear cells than non-hemolytic strains. Multiplicity of haemolysins appeared significant epidemiological tool. 相似文献
19.
A lectin was isolated from Galactia lindenii seeds and characterised. The lectin, purified by affinity chromatography, readily agglutinated O(H) human erythrocytes and interacted weakly with rabbit and rat erythrocytes. Specificity towards blood group H-type determinants was established; among them H-type 2 (alpha-L-Fuc (1-2)-beta-D-Gal (1-4)-beta-D-GlcNAc-O-R) was recognised by the lectin. The binding to the glycoconjugate was partially inhibited by GalNAc and Me-beta-Gal. The protein is an M=104,256 tetramer which dissociates into identical M=26,064 subunits under non-reducing conditions. Its amino acid composition, pI, A(1%), and N-terminal sequence (23 residues) were determined. The N-terminal region showed a unique sequence found hitherto only in some lectins (designated type-II) from the Dioclea genus. This work presents the evidence concerning a distinct type of lectin found in the Diocleinae tribe able to recognise the H-type 2 human blood group determinant and clearly different from the Glc/Man-specific lectins. The protein is a potential tool in cellular and histochemical studies. 相似文献
20.
观察3株双歧杆菌对人O型、人B型、绵羊、兔、鸡和小鼠红细胞的凝集滴度,并对其血凝素进行了提取。结果表明,3株双歧杆菌均能凝集各种来源的红细胞,血凝滴度无明显差异;D-甘露糖不能抑制血凝。提取的脂磷壁酸(LTA)具有血凝活性。其血凝素受体为糖类。 相似文献