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Characterisation of PtMYB1, an R2R3-MYB from pine xylem 总被引:2,自引:0,他引:2
Patzlaff A Newman LJ Dubos C Whetten RW Smith C McInnis S Bevan MW Sederoff RR Campbell MM 《Plant molecular biology》2003,53(4):597-608
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Differences in structure of adventitious roots in Salix clones with contrasting characteristics of cadmium accumulation and sensitivity 总被引:7,自引:0,他引:7
Glutamine synthetase (GS) genes, GS1a and GS1b, in pine ( Pinus sylvestris L.) are differentially regulated in tissue specificity and during seedling development. To gain insight into the regulatory mechanisms controlling their expression, we have analysed the 5'-flanking sequences of the gene GS1a using a transient expression system in pine protoplasts. Structural analysis of this region revealed the presence of putative regulatory elements including two AT-rich elements and a poly CT consensus sequence. A series of 5'- and 3'-deletions of the untranslated region covering the three putative elements, −800 to −626, −626 to −427 and +118 to +177 were analysed to demonstrate the functional implications of these elements in gene regulation. An electrophoretic mobility-shift assay showed that nuclear proteins prepared from pine cotyledons interact with both AT-rich regions (−800 to −427). Interestingly, no protein binding was detected when the untranslated region (+118 to +177) was included, even if deletion of that region suppressed promoter activity in the transient expression experiments conducted. However, simultaneous deletion of both types of cis elements, A/T and CT, resulted in a recovery of promoter activity of 50%. These results suggest a key regulatory role of the CT box by the interaction with A/T stretches in the distal part of the promoter and possibly with the proximal region (−427 to −1). 相似文献
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Involvement of Pinus taeda MYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis 总被引:2,自引:0,他引:2
Bomal C Bedon F Caron S Mansfield SD Levasseur C Cooke JE Blais S Tremblay L Morency MJ Pavy N Grima-Pettenati J Séguin A Mackay J 《Journal of experimental botany》2008,59(14):3925-3939
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Boudreau F Rings EH van Wering HM Kim RK Swain GP Krasinski SD Moffett J Grand RJ Suh ER Traber PG 《The Journal of biological chemistry》2002,277(35):31909-31917