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1.
Hypobaric hypoxia is a socio-economic problem affecting cognitive, memory and behavior functions. Severe oxidative stress caused by hypobaric hypoxia adversely affects brain areas like cortex, hippocampus, basal ganglia, and cerebellum. In the present study, we have investigated the antioxidant and memory protection efficacy of the synthetic NAP peptide (NAPVSIPQ) during long-term chronic hypobaric hypoxia (7, 14, 21 and 28 days, 25,000 ft) in rats. Intranasal supplementation of NAP peptide (2 μg/Kg body weight) improved antioxidant status of brain evaluated by biochemical assays for free radical estimation, lipid peroxidation, GSH and GSSG level. Analysis of expression levels of SOD revealed that NAP significantly activated antioxidant genes as compared to hypoxia exposed rats. We have also observed a significant increased expression of Nrf2, the master regulator of antioxidant defense system and its downstream targets such as HO-1, GST and SOD1 by NAP supplementation, suggesting activation of Nrf2-mediated antioxidant defense response. In corroboration, our results also demonstrate that NAP supplementation improved the memory function assessed with radial arm maze. These cumulative results suggest the therapeutic potential of NAP peptide for ameliorating hypobaric hypoxia-induced oxidative stress. 相似文献
2.
Kuan Zhang Tong Zhao Xin Huang Zhao-hui Liu Lei Xiong Ming-ming Li Li-ying Wu Yong-qi Zhao Ling-ling Zhu Ming Fan 《Cell stress & chaperones》2009,14(4):407-415
It has been shown that induction of HSP70 by administration of geranylgeranylacetone (GGA) leads to protection against ischemia/reperfusion
injury. The present study was performed to determine the effect of GGA on the survival of mice and on brain damage under acute
hypobaric hypoxia. The data showed that the mice injected with GGA survived significantly longer than control animals (survival
time of 9.55 ± 3.12 min, n = 16 vs. controls at 4.28 ± 4.29 min, n = 15, P < 0.005). Accordingly, the cellular necrosis or degeneration of the hippocampus and the cortex induced by sublethal hypoxia
for 6 h could be attenuated by preinjection with GGA, especially in the CA2 and CA3 regions of the hippocampus. In addition,
the activity of nitric oxide synthase (NOS) of the hippocampus and the cortex was increased after exposure to sublethal hypoxia
for 6 h but could be inhibited by the preinjection of GGA. Furthermore, the expression of HSP70 was significantly increased
at 1 h after GGA injection. These results suggest that administration of GGA improved survival rate and prevented acute hypoxic
damage to the brain and that the underlying mechanism involved induction of HSP70 and inhibition of NOS activity. 相似文献
3.
Sanchari Sinha Som Nath Singh Mantu Saha T. C. Kain A. K. Tyagi Uday Sankar Ray 《International journal of biometeorology》2010,54(1):85-92
High altitude (HA) is a multi-stressor environment comprising hypobaric hypoxia and cold. Climatic temperature varies with
seasonal variation at HA. The present study was undertaken to investigate the effect of ambient temperature on antioxidant
profile among sojourners at HA. The study was conducted on sojourners exposed to an altitude of 4,560 m in two different seasons
and categorized into two groups (SOJ 1, n = 63, ambient temp. at HA: −6o to +10oC; SOJ 2, n = 81, ambient temp. at HA: 3o–22oC). Blood was collected at sea level (SL) and after 4 weeks of HA exposure. Antioxidant
enzymes showed significant upregulation in SOJ 2 at HA. In SOJ 1, superoxide dismutase and glutathione peroxidase showed significant
upregulation but catalase and glutathione reductase showed significant decrease at HA. Non-enzymatic antioxidants showed significant
reduction in SOJ 1 whereas a sustained antioxidant profile was observed in SOJ 2 at HA. Oxidative stress markers showed higher
levels in SOJ 1 than SOJ 2 at HA. Differences observed between SOJ 1 and SOJ 2 at HA may be the consequence of different environmental
temperatures. Cold stress was higher in SOJ 1 as evidenced from the significantly lower oral temperature in SOJ 1 as compared
to SOJ 2. Cold- and hypoxia-induced increase in energy expenditure was significantly high in SOJ 1 than SOJ 2. To conclude,
chronic exposure to hypoxia in moderate climatic temperature has a potential preconditioning effect on antioxidant system,
but exposure to both cold and hypoxia causes greater oxidative stress due to altered metabolic rate. 相似文献
4.
Our previous study showed that pretreatment with 5-hydroxymethyl-2-furfural (5-HMF) led to protection against hypoxic injury
via a p-ERK-mediated pathway in vitro. Whether the protection of 5-HMF against hypoxia is effective in vivo is unknown. The
present study is aimed to verify the role of 5-HMF in acute hypobaric hypoxia using Kunming mice as an in vivo model and further
investigate the underlying mechanisms. Mice pretreated with or without 5-HMF for 1 h were exposed to acute hypobaric hypoxic
condition for 6 h and then the survival time, the survival rate, the permeability of blood–brain barrier (BBB), the histological
analysis in hippocampus and cortex, and the phosphorylation level of mitogen-activated protein kinases (ERK, JNK, and p38)
were investigated. The results showed that 5-HMF significantly increased the survival time and the survival rate of mice.
Accordingly, pretreatment with 5-HMF markedly attenuated acute hypobaric hypoxia-induced permeability of BBB (P < 0.01). In addition, the cellular damage extent of the hippocampus and the cortex induced by hypoxia for 6 h was also attenuated
by pretreatment with 5-HMF, especially in the hippocampus CA1 region. Furthermore, the activation of ERK rather than JNK and
p38 was involved in the protection of 5-HMF against acute hypobaric hypoxia. In summary, 5-HMF enhanced the survival capability
of mice and decreased acute hypoxic damage to the brain, which may be associated with the effects on BBB and p-ERK. 相似文献
5.
Yasmin Ahmad Narendra K. Sharma Mohammad Faiz Ahmad Manish Sharma Iti Garg Kalpana Bhargava 《PloS one》2014,9(5)
Background
Hypobaric hypoxia causes complex changes in the expression of genes, including stress related genes and corresponding proteins that are necessary to maintain homeostasis. Whereas most prior studies focused on single proteins, newer methods allowing the simultaneous study of many proteins could lead to a better understanding of complex and dynamic changes that occur during the hypobaric hypoxia.Methods
In this study we investigated the temporal plasma protein alterations of rat induced by hypobaric hypoxia at a simulated altitude of 7620 m (25,000 ft, 282 mm Hg) in a hypobaric chamber. Total plasma proteins collected at different time points (0, 6, 12 and 24 h), separated by two-dimensional electrophoresis (2-DE) and identified using matrix assisted laser desorption ionization time of flight (MALDI-TOF/TOF). Biological processes that were enriched in the plasma proteins during hypobaric hypoxia were identified using Gene Ontology (GO) analysis. According to their properties and obvious alterations during hypobaric hypoxia, changes of plasma concentrations of Ttr, Prdx-2, Gpx -3, Apo A-I, Hp, Apo-E, Fetub and Nme were selected to be validated by Western blot analysis.Results
Bioinformatics analysis of 25 differentially expressed proteins showed that 23 had corresponding candidates in the database. The expression patterns of the eight selected proteins observed by Western blot were in agreement with 2-DE results, thus confirming the reliability of the proteomic analysis. Most of the proteins identified are related to cellular defense mechanisms involving anti-inflammatory and antioxidant activity. Their presence reflects the consequence of serial cascades initiated by hypobaric hypoxia.Conclusion/Significance
This study provides information about the plasma proteome changes induced in response to hypobaric hypoxia and thus identification of the candidate proteins which can act as novel biomarkers. 相似文献6.
Keisho Katayama Yasitake Sato Koji Ishida Shigeo Mori Miharu Miyamura 《European journal of applied physiology and occupational physiology》1998,78(3):189-194
The present study was performed to investigate the effects of a combination of intermittent exposure to hypoxia during exercise
training for short periods on ventilatory responses to hypoxia and hypercapnia (HVR and HCVR respectively) in humans. In a
hypobaric chamber at a simulated altitude of 4,500 m (barometric pressure 432 mmHg), seven subjects (training group) performed
exercise training for 6 consecutive days (30 min · day−1), while six subjects (control group) were inactive during the same period. The HVR, HCVR and maximal oxygen uptake (V˙O2 max) for each subject were measured at sea level before (pre) and after exposure to intermittent hypoxia. The post exposure test
was carried out twice, i.e. on the 1st day and 1 week post exposure. It was found that HVR, as an index of peripheral chemosensitivity
to hypoxia, was increased significantly (P < 0.05) in the control group after intermittent exposure to hypoxia. In contrast, there was no significant increase in HVR
in the training group after exposure. The HCVR in both groups was not changed by intermittent exposure to hypoxia, while V˙O2 max increased significantly in the training group. These results would suggest that endurance training during intermittent exposure
to hypoxia depresses the increment of chemosensitivity to hypoxia, and that intermittent exposure to hypoxia in the presence
or absence of exercise training does not induce an increase in the chemosensitivity to hypercapnia in humans.
Accepted: 18 March 1998 相似文献
7.
Alireza Nakhaee Mohammad Bokaeian Azim Akbarzadeh Mohammad Hashemi 《Biological trace element research》2010,136(2):221-231
High blood glucose concentration in diabetes induces free radical production and, thus, causes oxidative stress. Damage of
cellular structures by free radicals play an important role in development of diabetic complications. In this study, we evaluated
effects of sodium tungstate on enzymatic and nonenzymatic markers of oxidative stress in brain of streptozotocin (STZ)-induced
diabetic rats. Rats were divided into four groups (ten rats in each group): untreated control, sodium tungstate-treated control,
untreated diabetic, and sodium tungstate-treated diabetic. Diabetes was induced with an intraperitoneal STZ injection (65 mg/kg
body weight), and sodium tungstate with concentration of 2 g/L was added to drinking water of treated animals for 4 weeks.
Diabetes caused a significant increase in the brain thiobarbituric acid reactive substances (P < 0.01) and protein carbonyl levels (P < 0.01) and a decrease in ferric reducing antioxidant power (P < 0.01). Moreover, diabetic rats presented a reduction in brain glucose-6-phosphate dehydrogenase (21%), superoxide dismutase
(41%), glutathione peroxidase (19%), and glutathione reductase (36%) activities. Sodium tungstate reduced the hyperglycemia
and restored the diabetes-induced changes in all mentioned markers of oxidative stress. However, catalase activity was not
significantly affected by diabetes (P = 0.4), while sodium tungstate caused a significant increase in enzyme activity of treated animals (P < 0.05). Data of present study indicated that sodium tungstate can ameliorate brain oxidative stress in STZ-induced diabetic
rats, probably by reducing of the high glucose-induced oxidative stress and/or increasing of the antioxidant defense mechanisms. 相似文献
8.
Bing Chen Ludwig V. Lamberts Geert J. Behets Tingting Zhao Mingxiang Zhou Gang Liu Xianghua Hou Guangju Guan Patrick C. D’Haese 《Biological trace element research》2009,131(1):1-12
Whole blood and serum samples of Chinese stable chronic renal failure (CRF) patients (n = 81), hemodialysis patients (n = 135), posttransplant patients (n = 60), and subjects with normal renal function (NRF; N = 42) were collected, as well as water and dialysate samples from five dialysis centers. The concentration of selenium (Se),
lead (Pb), and cadmium (Cd) was measured by atomic absorption spectrometry. The mean serum Se levels in patients with different
degrees of renal failure were significantly lower than those of subjects with NRF (p < 0.01). Pb levels were not increased in renal failure patients, while the Cd levels in patients with various degrees of
renal failure were higher than in subjects with NRF (p < 0.05). After correcting the results of Pb and Cd for hematocrit (Hct) however, Pb levels of dialysis patients were also
increased. In the dialysis population under study, blood Pb and Cd levels were closely related to the time on dialysis, while
contamination of the final dialysate may also contribute to the increased blood Cd and to a less extent Pb levels. Correction
for Hct may be recommended to accurately compare blood Pb and Cd levels in dialysis patients and CRF patients with varying
degrees of anemia to those of subjects with NRF. 相似文献
9.
Weiying Guo Dan Tian Ye Jia Wenlin Huang Mengnan Jiang Junnan Wang Weixia Sun Hao Wu 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》2018,1865(8):1034-1045
Oxidative stress and P53 contribute to the pathogenesis of diabetic kidney disease (DKD). Nuclear factor erythroid 2-related factor 2 (NRF2) is a master regulator of cellular antioxidant defense system, is negatively regulated by P53 and prevents DKD. Recent findings revealed an important role of mouse double minute 2 (MDM2) in protection against DKD. However, the mechanism remained unclear. We hypothesized that MDM2 enhances NRF2 antioxidant signaling in DKD given that MDM2 is a key negative regulator of P53. The MDM2 inhibitor nutlin3a elevated renal P53, inhibited NRF2 signaling and induced oxidative stress, inflammation, fibrosis, DKD-like renal pathology and albuminuria in the wild-type (WT) non-diabetic mice. These effects exhibited more prominently in nutlin3a-treated WT diabetic mice. Interestingly, nutlin3a failed to induce greater renal injuries in the Nrf2 knockout (KO) mice under both the diabetic and non-diabetic conditions, indicating that NRF2 predominantly mediates MDM2's action. On the contrary, P53 inhibition by pifithrin-α activated renal NRF2 signaling and the expression of Mdm2, and attenuated DKD in the WT diabetic mice, but not in the Nrf2 KO diabetic mice. In high glucose-treated mouse mesangial cells, P53 gene silencing completely abolished nutlin3a's inhibitory effect on NRF2 signaling. The present study demonstrates for the first time that MDM2 controls renal NRF2 antioxidant activity in DKD via inhibition of P53, providing MDM2 activation and P53 inhibition as novel strategies in the management of DKD. 相似文献
10.
We defined chronobiologic norms for supraventricular and ventricular single extrasystoles (SV and VE, respectively) in healthy
older males in lowland areas. The study was extended to higher altitudes, where hypobaric hypoxia was expected to increase
extrasystole frequency, while perhaps not changing rhythmicity. In healthy men (lowland n = 37, altitude n = 22), aged 49–72 years, mean numbers of SVs and VEs were counted over a 24-h period. Cosinor regression was used to test
the 24-h rhythm and its 2nd–10th harmonics. The resulting approximating function for either extrasystole type includes its
point, 95% confidence interval of the mean, and 95% tolerance for single measurement estimates. Separate hourly differences
(delta) between altitude and lowland (n = 59) were also analysed. Hourly means were significantly higher in the mountains versus lowland, by +0.8 beats/h on average
for SVs, and by +0.9 beats/h for VEs. A relatively rich chronogram for VEs in mountains versus lowland exists. Delta VEs clearly
display a 24-h component and its 2nd, 3rd, 4th and 7th harmonics. This results in significantly higher accumulation of VEs
around 8.00 a.m., 11.00 a.m. and 3.00 p.m. in the mountains. The increase in extrasystole occurrence in the mountains is probably
caused by higher hypobaric hypoxia and resulting sympathetic drive. Healthy men at elevated altitudes show circadian and several
ultradian rhythms of single VEs dependent on the hypoxia level. This new methodological approach—evaluating the differences
between two locations using delta values—promises to provide deeper insight into the occurrence of premature beats. 相似文献
11.
C. Schirlo A. Bub C. Reize A. Bührer J. Kohl E. A. Koller 《European journal of applied physiology and occupational physiology》1997,75(2):124-131
To investigate the role of fluid shifts during the short-term adjustment to acute hypobaric hypoxia (AHH), the changes in
lower limb (LV) and forearm volumes (FV) were measured using a strain-gauge plethysmograph technique in ten healthy volunteers
exposed to different altitudes (450 m, 2500 m, 3500 m, 4500 m) in a hypobaric chamber. Arterial blood pressure, heart rate,
arterial oxygen saturation (S
aO2), endtidal gases, minute ventilation and urine flow were also determined. A control experiment was performed with an analogous
protocol under normobaric normoxic conditions. The results showed mean decreases both in LV and FV of −0.52 (SD 0.39) ml · 100
ml−1 and −0.65 (SD 0.32) ml · 100 ml−1, respectively, in the hypoxia experiments [controls: LV −0.28 (SD 0.37), FV −0.41 (SD 0.47) ml · 100 ml−1]. Descent to normoxia resulted in further small but not significant decreases in mean LV [−0.02 (SD 0.11) ml · 100 ml−1], whereas mean FV tended to increase slightly [ + 0.02 (SD 0.14) ml · 100 ml−1]; in the control experiments mean LV and FV decreased continuously during the corresponding times [−0.19 (SD 0.31), −0.18
(SD 0.10) ml · 100 ml−1, respectively]. During the whole AHH, mean urine flow increased significantly from 0.84 (SD 0.41) ml · min−1 to 3.29 (SD 1.43) ml · min−1 in contrast to the control conditions. We concluded that peripheral fluid volume shifts form a part of the hypoxia-induced
acute cardiovascular changes at high altitude. In contrast to the often reported formation of peripheral oedema after prolonged
exposure to hypobaric hypoxia, the results provided no evidence for the development of peripheral oedema during acute induction
to high altitude. However, the marked increase in interindividual variance in S
aO2 and urine flow points to the appearance of the first differences in the short-term adjustment even after 2 h of acute hypobaric
hypoxia.
Accepted: 27 August 1996 相似文献
12.
Arsenic (As) toxicity through induction of oxidative stress is a well-known mechanism of organ toxicity. To address this problem,
buffalo epiphyseal proteins (BEP, at 100 μg/kg BW, i.p. for 28 days) were administered intraperitoneally to female Wistar
rats exposed to As (100 ppm sodium arsenite via drinking water for 28 days). Arsenic exposure resulted in marked elevation
in lipid peroxidation in brain, cardiac, and hepatic tissues, whereas significant (p < 0.05) adverse change in catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase, and reduced glutathione
level were observed in cardiac, hepatic, and brain tissues of As-administered animals. BEP significantly (p < 0.05) counteracted all the adverse changes in antioxidant defense system brought about by As administration. Based on these
results, we consider BEP as a potent antioxidant to be used for protection from arsenic-induced oxidative stress related damage
of vital organs. 相似文献
13.
Interleukin-18 gene polymorphism,but not interleukin-2 gene polymorphism,is associated with rheumatoid arthritis 总被引:1,自引:0,他引:1
To investigate whether polymorphisms of IL-2 and IL-18 genes are associated with rheumatoid arthritis (RA), polymorphisms of IL-2 and IL-18 genes were detected by polymerase-chain-reaction-based restriction analysis in the patients with RA and normal controls.
The results for the IL-18 gene revealed a significant difference between the patients and the normal controls (p = 0.000003), but there was no significant difference for the IL-2 gene (p = 0.876). The IL-18 gene 105A allele was associated with RA in Chinese patients. Individuals possessing the 105A allele had a higher incidence
of RA. A lack of association of IL-2 gene polymorphism between RA patients and healthy individuals was noted. The results of this study provide genetic evidence
that IL-18-105A/C polymorphism may play an effective role in RA. 相似文献
14.
15.
Khaled M. Al-Qudah Ahmad A. Gharaibeh Maysa’a M. Al-Shyyab 《Biological trace element research》2010,136(1):40-47
The aim of this study was to determine the levels of trace minerals Zn, Cu, and Se, the effect of dermatophytosis on the level
of thiobarbituric acid reactive substances (TBARS) as an indicator of lipid peroxidation, the status of enzymatic and nonenzymatic
antioxidants, and the relationship between the mentioned trace minerals and antioxidant defense system in calves with dermatophytosis.
A total of 21 Holstein calves with clinically established diagnosis of dermatophytosis and an equal number of healthy ones
were included in this study. Results showed that 81% of mycotic isolates were Trichophyton verrucosum, while 19% were Trichophyton mentagrophytes. The level of Zn, Cu, Se, and glutathione (GSH) and the activity of the antioxidant enzymes, glutathione peroxidase (GSH-Px),
and superoxide dismutase (SOD) were significantly (P ≤ 0.05) lower. The plasma level of TBARS was significantly (P ≤ 0.05) higher in dermatophytic calves compared to healthy controls. SOD activity was fairly correlated with serum Cu and
positively correlated with serum Zn in healthy control (r = 0.68, P ≤ 0.05; r = 0.58, P ≤ 0.05) and in calves affected with dermatophytosis (r = 0.73, P ≤ 0.05; r = 0.55, P ≤ 0.05), respectively. GSH-Px activity was highly correlated with whole blood selenium (r = 0.78, P ≤ 0.05) in healthy control and dermatophytic subjects (r = 0.76, P ≤ 0.05). Our results demonstrated that in dermatophytosis, the alteration in the antioxidant enzyme activities might be secondary
to changes in their cofactor concentrations. 相似文献
16.
Under hypobaric hypoxia, antioxidant defenses of the heart are stressed by the enhanced production of ROS. Mammalian heart acclimatizes to hypoxia through altered gene expression, which we studied in murine heart exposed to 10h of acute hypobaric hypoxia (AHH), equivalent to 15000ft, using cDNA arrays. Functional classification of genes with a > or =2-fold change revealed a number of pro-oxidants like Cyba, Xdh, Txnip, Ppp1r15b and antioxidants like Cat, Gpx1, Mt1, Mgst1. Interestingly, the protein level of Cyba, a subunit of NADPH oxidase, was markedly decreased in AHH exposed heart, suggesting the involvement of some stress response pathways. The AHH exposure also caused a significant reduction (50%) in the level of GSH (P<0.05). The present study provides a retrospective insight on the cellular antioxidant defense mechanisms under AHH. 相似文献
17.
Chintana Phawong Collins Ouma Piyatida Tangteerawatana Jarinee Thongshoob Tom Were Yuvadee Mahakunkijcharoen Duangrurdee Wattanasirichaigoon Douglas Jay Perkins Srisin Khusmith 《Immunogenetics》2010,62(6):345-356
Polymorphic variability in immune response genes, such as IL12B, encoding the IL-12p40 subunit is associated with susceptibility to severe malaria in African populations. Since the role
of genetic variation in conditioning severe malaria in Thai adults is largely unexplored, the functional association between
IL12B polymorphisms [i.e. IL12Bpro (rs17860508) and IL12B 3′ UTR T/G (rs3212227)], severe malaria and cytokine production was examined in patients with Plasmodium falciparum infections (n = 355) recruited from malaria endemic areas along the Thai–Myanmar border in northwest Thailand. Circulating IL-12p40 (p = 0.049) and IFN-γ (p = 0.051) were elevated in patients with severe malaria, while only IL-12p40 was significantly higher in severe malaria patients
with hyperparasitaemia (p = 0.046). Carriage of the IL12Bpro1.1 genotype was associated with enhanced severity of malaria (OR, 2.34; 95% CI, 0.94–5.81; p = 0.066) and hyperparasitaemia (OR, 3.42; 95% CI, 1.17–9.87; p = 0.025) relative to the IL12Bpro2.2 genotype (wild type). Individuals with the IL12Bpro1.1 genotype also had the lowest IL-12p40 (p = 0.002) and the highest IFN-γ (p = 0.004) levels. Construction of haplotypes revealed that carriage of the IL12Bpro-2/3′ UTR-T haplotype was associated with protection against severe malaria (OR, 0.51; 95% CI, 0.29–0.90; p = 0.020) and reduced circulating IFN-γ (p = 0.06). Thus, genotypic and haplotypic variation at IL12Bpro and IL12B 3′ UTR in this population influences susceptibility to severe malaria and functional changes in circulating IL-12p40 and
IFN-γ levels. Results presented here suggest that protection against severe malaria in Thai adults is associated with genotypic
variants that condition enhanced IL-12p40 and reduced IFN-γ levels. 相似文献
18.
Neelam Dhiman Iana H. Haralambieva Richard B. Kennedy Robert A. Vierkant Megan M. O’Byrne Inna G. Ovsyannikova Robert M. Jacobson Gregory A. Poland 《Immunogenetics》2010,62(4):197-210
An effective immune response to vaccination is, in part, a complex interaction of alleles of multiple genes regulating cytokine
networks. We conducted a genotyping study of Th1/Th2/inflammatory cytokines/cytokine receptors in healthy children (n = 738, 11–19 years) to determine associations between individual single-nucleotide polymorphisms (SNPs)/haplotypes and immune
outcomes after two doses of rubella vaccine. SNPs (n = 501) were selected using the ldSelect-approach and genotyped using Illumina GoldenGate™ and TaqMan assays. Rubella-IgG
levels were measured by immunoassay and secreted cytokines by ELISA. Linear regression and post hoc haplotype analyses were
used to determine associations between single SNPs/haplotypes and immune outcomes. Increased carriage of minor alleles for
the promoter SNPs (rs2844482 and rs2857708) of the TNFA gene were associated with dose-related increases in rubella antibodies. IL-6 secretion was co-directionally associated (p ≤ 0.01) with five intronic SNPs in the TNFRSF1B gene in an allele dose-related manner, while five promoter/intronic SNPs in the IL12B gene were associated with variations in IL-6 secretion. TNFA haplotype AAACGGGGC (t-statistic = 3.32) and IL12B promoter haplotype TAG (t-statistic = 2.66) were associated with higher levels of (p ≤ 0.01) rubella-IgG and IL-6 secretion, respectively. We identified individual SNPs/haplotypes in TNFA/TNFRSF1B and IL12B genes that appear to modulate immunity to rubella vaccination. Identification of such “genetic fingerprints” may predict
the outcome of vaccine response and inform new vaccine strategies. 相似文献
19.
Idiopathic pulmonary fibrosis (IPF) is a rare and devastating lung disease of unknown aetiology. Genetic variations in the
IL1RN gene, encoding the interleukin-1 receptor antagonist (IL-1Ra), have been associated with IPF susceptibility. Several studies
investigated the variable number tandem repeat (VNTR) or single nucleotide polymorphisms rs408392, rs419598 and rs2637988,
with variable results. The aim of this study was to elucidate the influence of polymorphisms in IL1RN on IPF susceptibility and mRNA expression. We performed a meta-analysis of the five case–control studies that investigated
an IL1RN polymorphism in IPF in a Caucasian population. In addition, we investigated whether IL1RN mRNA expression was influenced by IL1RN polymorphisms. The VNTR, rs408392 and rs419598 were in tight linkage disequilibrium, with D′ > 0.99. Furthermore, rs2637988 was in linkage disequilibrium with the VNTR (D′ = 0.90). A haploblock of VNTR*2 and the minor alleles of rs408392and rs419598 was constructed. Meta-analysis revealed that
this VNTR*2 haploblock is associated with IPF susceptibility both with an allelic model (odds ratio = 1.42, p = 0.002) and a carriership model (odds ratio = 1.60, p = 0.002). IL1RN mRNA expression was significantly influenced by rs2637988, with lower levels found in carriers of the (minor) GG genotype
(p < 0.001). From this meta-analysis, we conclude that the VNTR*2 haploblock is associated with susceptibility to IPF. In addition,
polymorphisms in IL1RN influence IL-1Ra mRNA expression, suggesting that lower levels of IL-1Ra predispose to developing IPF. Together these findings
demonstrate that the cytokine IL-1Ra plays a role in IPF pathogenesis. 相似文献
20.
Akhileshwar Namani Yulong Li Xiu Jun Wang Xiuwen Tang 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》2014
Nuclear factor-erythroid 2 p45-related factor 2 (NRF2, also known as Nfe2l2) plays a critical role in regulating cellular defense against electrophilic and oxidative stress by activating the expression of an array of antioxidant response element-dependent genes. On one hand, NRF2 activators have been used in clinical trials for cancer prevention and the treatment of diseases associated with oxidative stress; on the other hand, constitutive activation of NRF2 in many types of tumors contributes to the survival and growth of cancer cells, as well as resistance to anticancer therapy. In this review, we provide an overview of the NRF2 signaling pathway and discuss its role in carcinogenesis. We also introduce the inhibition of NRF2 by nuclear receptors. Further, we address the biological significance of regulation of the NRF2 signaling pathway by nuclear receptors in health and disease. Finally, we discuss the possible impact of NRF2 inhibition by nuclear receptors on cancer therapy. 相似文献