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1.
Summary A variety of human tendons have been studied at the electron microscope level. The fibers of these tendons are composed of collagen fibrils that average 1,750 Å and 600 Å in diameter. A third population that measures 100 Å in diameter may represent immature collagen or filaments that are incorporated into tendon elastic fibers. The larger collagen fibrils vary in ratio with respect to one another, and are connected by interfibrillar bridges which in some cases appear to extend through the substance of the fibril. The collagen fibrils of the paratenon are less-well organized than those of the tendon proper and average 600 Å in diameter. Tendons that exhibit the property of lateral stretch (plantaris and palmaris) were compared at the ultrastructural level with tendons that do not have this property. No differences between the two tendon types could be determined in normal or spread preparations, indicating that the differences in physical characteristics are a result of fiber rather than fibril organization.Supported by Edward G. Schlieder Foundation GrantThe authors wish to thank Mrs. Janell Buck and Mrs. Eunice Schwartz for their excellent technical and secretarial assistance, and Mr. Garbis Kerimian for his excellent photographic work 相似文献
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Kedar N. Prasad Erika Carvalho Judith Edwards-Prasad Francisco G. La Rosa Sanjay Kumar Jae Ho Kim Arlen Meyers Susan Kentroti 《In vitro cellular & developmental biology. Animal》1994,30(5):312-320
Summary This study reports the establishment ofα-amylase-producing human parotid pleomorphic adenoma cell lines (2HP and 2HP1) which have been maintained in culture for over 1 yr. The procedures required preparation of cellular clumps from tumor tissue
and plating them on plasma clot or precoated dishes. During the initial phase of growth they required modified MCDB-153 medium
without serum. When cells showed signs of degeneration they were changed to MCDB-153 medium containing first 2% and then 10%
heat inactivated fetal bovine serum. Although cells grew well in MCDB-153 containing 10% serum, the epithelial cell morphology
was not distinct. Therefore, the growth and morphology of cells grown in MCDB-10% serum were compared with those in RPMI growth
medium containing 10% fetal bovine serum and F12 containing 10% agammaglobulin newborn bovine serum. Although the growth of
cells was a little slower in F12 medium than those in MCDB and RPMI, the epithelial cell morphology was maintained better
than in other growth media. The cells of 2HP and 2HP1 produce low levels ofα-amylase and relatively high levels ofα-amylase mRNAs of 1176 and 702 bp and contain neurofilament-160, a neuronal-specific marker. The cells of 2HP1 are tumorigenic when tested in athymic mice, but the cells of 2HP are not. The establishment of amylase-producing human parotid
adenoma cell lines of different characteristics in culture provides a new opportunity to study the mechanisms of differentiation
and transformation, and regulation ofα-amylase in these cells. 相似文献
4.
Mary K.B. McGuire-Goldring Gillian Murphy Maxine Gowen Judith E. Meats Nicolette M. Ebsworth Christopher Poll John J. Reynolds R.Graham G. Russell 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1983,763(2):129-139
Human articular chondrocytes in culture produced large amounts of specific mammalian collagenase, gelatinase and proteoglycanase when exposed to dialysed supernatant medium derived from cultured human blood mononuclear cells (mononuclear cell factor) or to conditioned medium, partially purified by fractionation with ammonium sulphate (60–90% fraction), from cultures of human synovial tissue (synovial factor). Human chondrocytes and synovial cells also released into culture medium an inhibitor of collagenase of apparent molecular weight about 30 000, which appeared to be similar to the tissue inhibitor of metalloproteinases synthesised by tissues in culture. The amounts of free collagenase inhibitor were reduced in culture media from chondrocytes or synovial cells exposed to mononuclear cell factor or synovial factor. While retinol inhibited the production of collagenase brought about by mononuclear cell factor or synovial factor, it restored the levels of inhibitor, which were reduced in the presence of mononuclear cell factor or synovial factor. Dexamethasone markedly reduced the production of collagenase by synovial cells, while only partially inhibiting factor-stimulated collagenase production by chondrocytes. Addition of puromycin as an inhibitor of protein synthesis reduced the amounts of both collagenase and inhibitor to control or undetectable levels. 相似文献
5.
Extensive data on the glycosaminoglycan (GAG) composition and the collagen fibril diameter distribution have been collected for a diverse range of connective tissues. It is shown that tissues with the smallest diameter collagen fibrils (mass-average diameter less than 60 nm) have high concentrations of hyaluronic acid and that tissues with the largest diameter collagen fibrils (mass-average diameter approximately 200 nm) have high concentrations of dermatan sulphate. It is suggested that the lateral growth of fibrils beyond a diameter of about 60 nm is inhibited by the presence of an excess of hyaluronic acid but that this inhibitory effect may be removed by an increasing concentration of chondroitin sulphate and/or dermatan sulphate. It is also postulated that high concentrations of chondroitin sulphate will inhibit fibril growth beyond a mass-average diameter of approximately 150 nm. Such an inhibition may in turn be removed by an increasing concentration of dermatan sulphate such that it becomes the dominant GAG present in the tissue. 相似文献
6.
Dr. James G. Tidball 《Cell and tissue research》1982,222(3):635-645
Summary Electron-microscopical autoradiography and cytochemical techniques have been used to identify the distinct and separate subcellular structures involved in the secretion of 1) procollagen, 2) dihydroxyphenylalanine (DOPA), which is a precursor of a collagen-crosslinking compound, and 3) DOPA oxidase, which converts DOPA to a putative crosslinking compound of collagen in the axial skeleton of the gorgonian coral Leptogorgia virgulata. Some skeletal-protein hydrolysates contain material that co-elutes with DOPA. The data indicate that these skeletogenic cells, corticocytes, are capable of modifying the number of non-reducible, tyrosine-derived crosslinkages of collagen by the secretion of a crosslinking compound that acts extracellularly on collagen. A mechanism for a cell-mediated control of the mechanical properties of collagen is thereby presented. 相似文献
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Organotypic culture of outer root sheath cells from human hair follicles using a new culture device 总被引:2,自引:0,他引:2
Summary A new culture vessel for the growth of cells on biological substrata and under organotypic conditions is described. This device,
named Combi-ring-dish (CRD), is composed of four concentric rings designed to take up one or several substrata on which cells
can be grown either immersed in culture medium or exposed to air and fed from underneath. Using the CRD, outer root sheath
cells, isolated from plucked human hair follicles and plated on growth-arrested 3T3 feeder layers, were grown on native collagen
lattices populated with living human fibroblasts. After reaching confluence, the immersed cultures were recombined (in vitro)
with pieces of freeze-killed dermis and grown further, exposed to air. Thus by mimicking epidermal growth conditions, differentiation
was dramatically improved, compared to control cultures on plastic substratum. Virtually all morphologic features of interfollicular
epidermis developed. This seems a suitable model to investigate the differentiation potential of human hair follicle cells. 相似文献
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García-Sancho M 《Studies in History and Philosophy of Science Part C: Studies in History and Philosophy of Biological and Biomedical Sciences》2011,42(2):233-245
This paper explores the different identities adopted by connective tissue research at the University of Manchester during the second half of the 20th century. By looking at the long-term redefinition of a research programme, it sheds new light on the interactions between different and conflicting levels in the study of biomedicine, such as the local and the global, or the medical and the biological. It also addresses the gap in the literature between the first biomedical complexes after World War II and the emergence of biotechnology. Connective tissue research in Manchester emerged as a field focused on new treatments for rheumatic diseases. During the 1950s and 60s, it absorbed a number of laboratory techniques from biology, namely cell culture and electron microscopy. The transformations in scientific policy during the late 70s and the migration of Manchester researchers to the US led them to adopt recombinant DNA methods, which were borrowed from human genetics. This resulted in the emergence of cell matrix biology, a new field which had one of its reference centres in Manchester. The Manchester story shows the potential of detailed and chronologically wide local studies of patterns of work to understand the mechanisms by which new biomedical tools and institutions interact with long-standing problems and existing affiliations. 相似文献
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Microencapsulated human bone marrow cultures: a potential culture system for the clonal outgrowth of hematopoietic progenitor cells 总被引:1,自引:0,他引:1
Currently the most successful methods for culturing human hematopoietic cells employ some form of perfused bioreactor system. However, these systems do not permit the clonal outgrowth of single progenitor cells. Therefore, we have investigated the use of alginate-poly-L-lysine microencapsulation of human bone marrow, combined with rapid medium exchange, as a system that may overcome this limitation for the purpose of studying the kinetics of progenitor cell growth. We report that a 12 to 24-fold multilineage expansion of adult human bone marow cells was achieved in about 16 to 19 days with this system and that visually identifiable colonies within the capsules were responsible for the increase in cell number. The colonies that represented the majority of cell growth originated from cells that appeared to be present in a frequency of about 1 in 4000 in the encapsulated cell population. These colonies were predominantly granulocytic and contained greater than 40,000 cells each. Large erythroid colonies were also present in the capsules, and they often contained over 10,000 cells each. Time profiles of the erythroid progenitor cell density over time were obtained. Burst-forming units erythroid (BFU-E) peaked around day 5, and the number of morphologically identifiable erythroid cells (erythroblasts through reticulocytes) peaked on day 12. We also report the existence of a critical inoculum density and how growth was improved with the use of conditioned medium derived from a microcapsule culture initiated above the critical inoculum density. Taken together, these results suggest that microencapsulation of human hematopoietic cells allows for outgrowth of progenitor, and possible preprogenitor, cells and could serve as a novel culture system for monitoring the growth and differentiation kinetics of these cells. 相似文献
12.
Giovanni Candiano Rosanna Gusmano Paola Altieri Roberta Bertelli Fabrizio Ginevri Domenico A. Coviello Adalberto Sessa Gianluca Caridi Gian Marco Ghiggeri 《Virchows Archiv. B, Cell pathology including molecular pathology》1993,63(1):1-9
Cells from the cysts of patients with autosomal dominant polycystic kidney disease (PKD) were grown in vitro under standard
conditions without the aid of collagen-pretreated surfaces, and both the synthesis and composition of the extracellular matrix
were investigated. At confluence, PKD cells presented the typical features of epithelial cells, but showed a different collagen
composition from fibroblasts. Compared with normal tubular epithelia (NTE), PKD monolayers produced an excess of extracellular
matrix, which accounted for 30% of the total incorporation of [3H] proline, although this value was considerably lower (by a factor of 10) in the case of NTE. Immunohistochemical and electrophoretic
techniques revealed a complex collagen composition in the extracellular matrix which included [α(III)]3 and collagen IV. However, part of the collagen components remained unidentified in spite of the fact that they exhibited
a typical Mr of α1(I) and α2(I) in the presence of urea. Immunoprecipitation with monospecific antibodies and Northern blotting with specific probes failed
to recognize α1(I) and α2(I), but demonstrated their presence in fibroblasts. Purification and cyanogen bromide digestion demonstrated a strong interhomology
in fingerprint peptide composition among the uncharacterized collagens synthesized by PKD cells, thus suggesting a common
identity. These observations document a markedly augmented production of extracellular matrix by PKD cultured cells in vitro,
and show the presence of collagens which do not share homologies with the major collagen molecules. A better characterization
of extracellular matrix composition is central to any comprehension of the cystogenetic mechanisms in vivo. 相似文献
13.
乙酰胆碱对三种人垂体腺瘤细胞增殖及凋亡的影响 总被引:2,自引:2,他引:0
为了解乙酰胆碱(acetylcholine,ACh)在人垂体腺瘤发生、发展中的作用,本研究首先观察了人垂体腺瘤细胞内是否存在合成ACh必需的胆碱乙酰转移酶,并应用MTT实验、[^3H]TdR掺入实验、细胞周期分析和TUNEL法测定了ACh对体外培养的人垂体无功能瘤、催乳素瘤和生长激素瘤等三种瘤细胞增殖和凋亡的影响。结果发现:(1)三种垂体腺瘤细胞中均有胆碱酯酶的表达,但明显少于正常垂体;(2)ACh对三种类型的人垂体腺瘤细胞增殖代谢的影响相类似,不同浓度的ACh能明显抑制体外培养的三种人垂体腺瘤细胞的增殖,呈明显的剂量效应关系,同时ACh能减少垂体腺瘤细胞进入S、G2期的细胞比例,而使处于G1期的细胞比例增加;(3)ACh的这种作用可被阿托品阻断,但不受筒箭毒的影响;(4)ACh对体外培养的三种人垂体腺瘤细胞凋亡无明显影响。该结果提示,ACh可能以旁分泌或自分泌的方式作用于垂体前叶细胞,对垂体腺瘤细胞增殖分化有调控作用,而且是通过ACh M受体来实现的。 相似文献
14.
Summary The cell surface differentiation antigen, Thy-1, has been demonstrated by immunofluorescence to be associated with some collagen-based connective tissue. In the rat, the basement membrane of kidney collecting tubules and of certain blood vessels, reticulin of the lamina propria, loose connective tissue in the dermis, and collagen fibres within certain nerve cell tumours, bear the antigen. Other, apparently similar, connective tissue (especially that of muscle and liver) lacked Thy-1. In the mouse, only the connective tissue of the skin was found to bear the antigen. The possible origin of this extracellular Thy-1, and its implications for the function of the molecule, are discussed.Part of this work was supported by grant no. NS10861 from the National Institute of Health and no. BNS 77-15818 from the National Science Foundation, awarded to Dr. S.E. Pfeiffer 相似文献
15.
Wenli Zhang Caibin Li Bruce C. Baguley Fang Zhou Weisai Zhou John P. Shaw Zhen Wang Zimei Wu Jianping Liu 《Analytical biochemistry》2016
To obtain a multicellular MCF-7 spheroid model to mimic the three-dimensional (3D) of tumors, the microwell liquid overlay (A) and hanging-drop/agar (B) methods were first compared for their technical parameters. Then a method for embedding spheroids within collagen was optimized. For method A, centrifugation assisted cells form irregular aggregates but not spheroids. For method B, an extended sedimentation period of over 24 h for cell suspensions and increased viscosity of the culture medium using methylcellulose were necessary to harvest a dense and regular cell spheroid. When the number was less than 5000 cells/drop, embedded spheroids showed no tight cores and higher viability than the unembedded. However, above 5000 cells/drop, cellular viability of embedded spheroids was not significantly different from unembedded spheroids and cells invading through the collagen were in a sun-burst pattern with tight cores. Propidium Iodide staining indicated that spheroids had necrotic cores. The doxorubicin cytotoxicity demonstrated that spheroids were less susceptible to DOX than their monolayer cells. A reliable and reproducible method for embedding spheroids using the hanging-drop/agarose method within collagen is described herein. The cell culture model can be used to guide experimental manipulation of 3D cell cultures and to evaluate anticancer drug efficacy. 相似文献
16.
John M. Quarles Nell G. Morris Albert Leibovitz 《In vitro cellular & developmental biology. Plant》1980,16(2):113-118
Summary Four human colorectal adenocarcinoma tumor cell lines, previously established and characterized in monolayer culture were
grown in a matrix-perfusion culture system to determine the suitability of this technique for synthesis of carcinoembryonic
antigen (CEA). Production of CEA in excess of 100,000 ng was attained from one cell line, SW 403, during 15-day growth trials.
In growth trials and cell-free diffusion studies, CEA passed through membranes of 100,000-dalton molecular weight porosity
but not 10,000 porosity. Using cell cultures of high, moderate, or low producers, CEA synthesis tended to reach a plateau
after several days of culture and remained nearly constant as the cells attained a maintenance condition. Basic biologic characteristics
of the cell lines, expressed as growth rates and CEA produced per 106 cells, were comparable in monolayer and perfusion culture. The high cell densities, (108 to 109 cells per ml) achieved in matrix perfusion made it possible to routinely obtain continuous high yields of CEA over an extended
time period.
Presented in part at the 29th Annual Meeting of the Tissue Culture Association, Denver, Colorado, June 4–8, 1978.
This work was supported in part by the fund for Organized Research, State of Texas, and a grant from Scott and White Clinic. 相似文献
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Kubota S Kawaki H Kondo S Yosimichi G Minato M Nishida T Hanagata H Miyauchi A Takigawa M 《Biochimie》2006,88(12):1973-1981
CCN2 consists of 4 distinct modules that are conserved among various CCN family protein members. From the N-terminus, insulin-like growth factor binding protein (IGFBP), von Willebrand factor type C repeat (VWC), thrombospondin type 1 repeat (TSP1) and C-terminal cysteine-knot (CT) modules are all aligned tandem therein. The multiple functionality of CCN2 is thought to be enabled by the differential use of these modules when interacting with other molecules. In this study, we independently prepared all 4 purified module proteins of human CCN2, utilizing a secretory production system with Brevibacillus choshinensis and thus evaluated the cell biological effects of such single modules. In human umbilical vascular endothelial cells (HUVECs), VWC, TSP and CT modules, as well as a full-length CCN2, were capable of efficiently activating the ERK signal transduction cascade, whereas IGFBP was not. In contrast, the IGFBP module was found to prominently activate JNK in human chondrocytic HCS-2/8 cells, while the others showed similar effects at lower levels. In addition, ERK1/2 was modestly, but significantly activated by IGFBP and VWC in those cells. No single module, but a mixture of the 4 modules provoked a significant activation of p38 MAPK in HCS-2/8 cells, which was activated by the full-length CCN2. Therefore, the signals emitted by CCN2 can be highly differential, depending upon the cell types, which are thus enabled by the tetramodular structure. Furthermore, the cell biological effects of each module on these cells were also evaluated to clarify the relationship among the modules, the signaling pathways and biological outcomes. Our present results not only demonstrate that single CCN2 modules were potent activators of the intracellular signaling cascade to yield a biological response per se, while also providing new insight into the module-wise structural and functional relationship of a prototypic CCN family member, CCN2. 相似文献
18.
Scanning electron microscopy of bone cells in culture 总被引:1,自引:0,他引:1
Summary Embryonic and young rat bone cells have been grown in culture and examined in the scanning electron microscope (SEM). Compared with cells fixed in situ and taken directly from the animal, the cultured osteoblastic cells were smoother, flatter and more extensive and showed tighter intercellular contacts. Some matrix is formed in culture and undergoes at least partial mineralization as judged by the accumulation of Ca and P measured by energy dispersive x-ray analysis. Findings concerning the morphology of the collagen arrangement were indecisive. Some superficial cells, free of surrounding matrix, resembled osteocytes in normal in vivo bone. This may indicate that a proportion of the extracellular matrix produced by the cultured cells failed to polymerise into recognizable bone matrix, and that osteocytic morphology is not dependent upon the physical characteristics of the bone matrix. 相似文献
19.
Maintenance and characterization of human myometrial smooth muscle cells in monolayer culture 总被引:4,自引:0,他引:4
M. Linette Casey Paul C. MacDonald Murray D. Mitchell Jeanne M. Snyder 《In vitro cellular & developmental biology. Plant》1984,20(5):396-403
Summary Human myometrial cells were dispersed from uterine tissue by limited enzymatic digestion of myometrium that was obtained at
the time of hysterectomy. The dispersed myometrial cells that are obtained in this manner can be maintained in monolayer culture
in the presence of medium that contains fetal bovine serum. In primary culture, as well as after passage, the characteristics
of these cells are morphologically and biochemically similar to those of smooth muscle cells and myometrial tissue.
This investigation was supported, in part, by U.S. Public Health Service Grants 5-P50-HD11149 and 5-P01-AG00306. 相似文献
20.
Epigenetic and posttranslational modifications of the expression of cell cycle-relevant genes or proteins like p21, e.g., by miRNAs are crucial mechanisms in the development or prevention of colon cancer. The present study investigated the influence of butyrate and trichostatin A (TSA) as histone deacetylase inhibitors on the expression of colon cancer-relevant miRNA (miR-135a, miR-135b, miR-24, miR-106b, miR-let-7a) in LT97 colon adenoma cells as a model of an early stage of colon carcinogenesis. The impact of distinct miRNAs (miR-106b, miR-135a) on butyrate-mediated regulation of p21 and Cyclin D2 gene and protein expression as well as the effect on LT97 cell proliferation (non-transfected, miR-106b and miR-135a mimic transfected) was analyzed. Butyrate and partial TSA reduced the expression of miR-135a, miR-135b, miR-24 and miR-let-7a (~0.5-fold, 24 h) and miR-24, miR-106b and miR-let-7a (~0.5–0.7-fold, 48 h) in LT97 cells. Levels of p21 mRNA and protein were significantly increased by butyrate and TSA (~threefold and 4.5-fold, respectively, 24 h) in non-transfected but not in miR-106b transfected LT97 cells. Levels of Cyclin D2 mRNA were significantly reduced by butyrate and TSA (~0.3-fold, 24 h) in non-transfected and miR-135a-transfected LT97 cells, whereas protein levels were predominantly not influenced. MiR-106b and miR-135a significantly reduced butyrate-/TSA-mediated inhibition of LT97 cell proliferation (72 h). These results indicate that butyrate is able to modify colon cancer-relevant miRNAs like miR-106b and miR-135a which are involved in the regulation of cell cycle-relevant genes like p21 and might influence inhibition of adenoma cell proliferation.