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1.
提高小麦愈伤组织分化频率的因素 总被引:52,自引:0,他引:52
研究了影响小麦愈伤组织诱导、芽分化及其植株再生的一些因素。结果表明:在愈伤组织诱导和继代过程中添加ABA(1.0mg/L)有利于小麦中晚期幼胚致密愈伤组织的诱导及再生能力的保持;外植体来源尤其是基因型对长期培养的愈伤组织再生能力有很大影响;不同的外源激素(KT、6-BA、IAA、TDZ和玉米素等)也影响芽分化频率,其中TDZ可明显提高芽分化频率;在转入分化培养前对愈伤组织进行干燥处理可有效地提高其 相似文献
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付丽张东向刘安奇吕晴李伟 《生物技术》2020,(6):569-578
[目的]研究防风愈伤组织形成和分化的适宜条件,为优良品种的培育奠定基础。[方法]以防风幼苗为材料,进行愈伤组织诱导和分化研究。[结果]以茎段为外植体在黑暗下诱导愈伤组织分化最佳;在黑暗条件下继代,最佳培养基为MS+2,4-D 0.6 mg/L+6-BA 0.4 mg/L+KT 0.6 mg/L。光暗交替下MS+1.0 mg/L KT+0.4 mg/L NAA和MS+NAA 0.2 mg/L+6-BA 0.4 mg/L+KT 0.6 mg/L为最佳丛生芽诱导培养基;黑暗下MS+NAA 0.6 mg/L+6-BA0.5 mg/L和MS+NAA 1.0 mg/L+KT 0.6 mg/L为最佳不定根诱导培养基。且MS+NAA 1.0 mg/L+6-BA 0.5 mg/L和MS+NAA 0.6 mg/L+6-BA 0.4 mg/L+KT 0.2 mg/L均有利于防风丛生芽的诱导,NAA 0.2 mg/L+IAA 0.2 mg/L+IBA 0.2 mg/L有利于防风不定根的形成。[结论]成功诱导防风愈伤组织并分化出丛生芽和不定根,为再生体系建立和规模化培养提供技术支撑。 相似文献
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翅果油树茎段愈伤组织和芽发生的组织的学研究 总被引:3,自引:0,他引:3
本文对翅果油树大宫灯型茎段培养在MS附加6-BA较高,NAA较低浓度的培养基上培养0-30d的组织学变化进行了研究。创伤对其愈伤组织的形成有明显的刺激作用,培养3-4d切口处的皮层细胞,形成层细胞,韧皮部薄壁细胞以及髓组织细胞,甚至表皮细胞均脱化分化开始分裂; 相似文献
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提高小麦愈伤组织分化频率的因素 总被引:4,自引:0,他引:4
研究了影响小麦愈伤组织诱导、芽分化及其植株再生的一些因素。结果表明:在愈伤组织诱导和继代过程中添加ABA(1 .0mg/L) 有利于小麦中晚期幼胚致密愈伤组织的诱导及再生能力的保持;外植体来源尤其是基因型对长期培养的愈伤组织再生能力有很大影响; 不同的外源激素( KT、6BA、IAA、TDZ和玉米素等) 也影响芽分化频率,其中TDZ可明显提高芽分化频率;在转入分化培养前对愈伤组织进行干燥处理可有效地提高其芽分化频率;在生根培养基中添加适量的IAA 或NAA 可有效促进生根。 相似文献
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以油桐无菌苗叶片为试材,研究不同种类及浓度的植物生长调节剂对愈伤组织诱导、分化、增殖及生根的影响。结果表明:叶片愈伤组织的最佳诱导培养基为1/2MS+2.omg·L-16-BA+1.0mg·L~2,4-D,诱导率达100%;最佳分化培养基为1/2MS+3.0mg·L~6-BA+0.1mg·L-1。IBA+0.05mg·L—IAA,分化率为86.36%;最佳继代增殖培养基MS+3.0mg·L~6-BA+0.05mg·L。IBA;最佳生根培养基为1/2MS+O.1mg·L-1。IBA,生根率93.83%。炼苗后移栽到泥炭土:珍珠岩:蛭石=2:1:1的基质中,成活率达92%以上。 相似文献
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小麦愈伤组织及再生植株的染色体变异 总被引:20,自引:0,他引:20
对培养在含有不同附加成分的MS培养基上的小麦愈伤组织染色体进行了跟踪研究。结果表明,在整个培养过程中各培养基上愈伤组织都有一定程度的染色体变异。在培养初期,高浓度2,4-D可增加愈伤组织中的染色体变异率,AgNO_3可降低染色体变异率。6-BA对培养初期愈伤组织染色体变异率没有显著影响。但高浓度6-BA可加大长期培养愈伤组织中的超倍体细胞频率。蔗糖浓度对最初9代愈伤组织染色体变异率无显著影响。但之后,低浓度蔗糖培养基上亚倍体细胞频率明显减小。随着培养时间的延长,各培养基上愈伤组织中正常二倍体细胞的频率都有逐渐上升的趋势。在再生植株中,大部分核型正常,只有少数植株具有染色体数目或结构变异。有些核型正常植株也有表型变异。 相似文献
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小麦叶片愈伤组织及其再生植株的诱导 总被引:17,自引:0,他引:17
小麦幼苗基部外植体在补加2,4-D的MS、N6、BA1(3)培养基上均可诱导出愈伤组织,2,4-D的最适浓度为2.0mg/L;愈伤组织的增殖速度与切段部位及基本培养基有关,其中在以MS补加2.0mg/L2,4-D的培养基上诱导出的愈伤组织增长速度最快;最后讨论了小麦愈伤组织幼苗诱导率低的原因及可能解决的方法。 相似文献
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The comparative uptake of four perfluorinated carboxylic acids (PFCAs) by wheat (Triticum aestivum L.) grown in nutrient solution was investigated. Wheat is the main food crop in northern China and may become a potential pathway of human exposure to these PFCAs. The uptake of four PFCAs from water at a fixed concentration (1 μg/mL) increased over time, approaching a steady state, and except for the short-chain perfluorobutanoic acid, most of the total mass of each of the PFCAs taken up by wheat was found to be at the root. The root concentration factor (RCF) and shoot/root concentration factor (SRCF) were calculated, and with the increase in carbon chain length, the RCFs increased but SRCFs decreased, which indicated that long-chain PFCAs had stronger root uptake and weaker translocation capacities than short-chain PFCAs. In addition, pH could obviously impact the uptake of four PFCAs in the roots and shoots of wheat, and the highest concentrations were found at pH = 7 when the pH increased from 4 to 10. 相似文献
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Gravitropic response growth angle and vertical distribution of roots of wheat (Triticum aestivum L.)
Recent work on root distribution, growth angles and gravitropic responses in Japanese cultivars of winter wheat are reviewed. Vertical distribution of roots, which influences the environmental stress tolerance of plants, was observed in the 12 Japanese cultivars in the field. The root depth index (RDI: the depth at which 50% of the root length has been reached) differed among the cultivars at the stem elongation stage. Since the RDI was closely related to the growth angle of seminal roots obtained in a pot experiment, it was assumed that growth angle is useful for predicting vertical root distribution among wheat genotypes. Gravitropic responses of the primary seminal root of 133 Japanese wheat cultivars assessed by measuring the growth angle in agar medium, were larger in the northern Japanese cultivars and smaller in the southern ones. It was also found that the geographical variation resulted from the wheat breeding process, i.e. genotypes with limited gravitropic responses of roots had been selected in the southern part of Japan where excessive soil moisture is one of the most serious problems. 相似文献
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A new, endosperm-supported callus induetion method was developed using mesocotyls of mature wheat embryos. After seed germination under aseptic condition, most of the germ tissues were cut off and only a few mm of the mesocotyl tissue with the scutellum was used for callus induction. The seeds were placed furrow downwards in 2,4-D solution (6–8 mg l-1). Proliferating callus tissues were already observed on the cut surface of the mesocotyls on the 2nd day after inoculation. On the MS nutrient medium, callus formation from the isolated scutella with attached mesocotyls was negligible even after 6 days. For shoot and root regeneration, the calli produced up to 10 days were removed from the seeds and transferred onto a hormone-free MS medium. As shown by histological methods, the plantlets regenerated via organogenesis. 相似文献
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Silicon absorption by wheat (Triticum aestivum L.) 总被引:3,自引:0,他引:3
Although silicon (Si) is a quantitatively major inorganic constituent of higher plants the element is not considered generally
essential for them. Therefore it is not included in the formulation of any of the solution cultures widely used in plant physiological
research. One consequence of this state of affairs is that the absorption and transport of Si have not been investigated nearly
as much as those of the elements accorded 'essential' status. In this paper we report experiments showing that Si is rapidly
absorbed by wheat (Triticum aestivum L.) plants from solution cultures initially containing Si at 0.5 mM, a concentration
realistic in terms of the concentrations of the element in soil solutions. Nearly mature plants (headed out) 'preloaded' with
Si absorbed it at virtually the same rate as did plants grown previously in solutions to which Si had not been added. The
rate of Si absorption increased by more than an order of magnitude between the 2-leaf and the 7-8 leaf stage, with little
change thereafter.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Plant regeneration was achieved from coleoptile tissue of wheat (Triticum aestivum L. cv. Kharachia-65). Coleoptiles (1.0
- 3.5 cm long) were excised from 2- to 5-d-old seedlings and cultured on Murashige and Skoog's (MS) medium supplemented with
2,4-dichlorophenoxyacetic acid (2,4-D - 0.5, 2.5, and 5.0 mg dm-3). Cream, friable callus was obtained after 6 weeks of inoculation. This callus was sub-cultured on MS medium supplemented
with 2,4-D (2.5 mg dm-3) and 5 % coconut water. After 6 weeks of sub-culturing white, cream or pale, friable, nodular callus was obtained. Plant
regeneration occurred when this callus was sub-cultured on MS medium supplemented with 0.2 mg dm-3 1-naphthalene acetic acid + 1.0 mg dm-3 6-benzylaminopurine. For rooting, regenerated shoots or plantlets were transferred on MS medium supplemented with 0.5 mg
dm-3 indole-3-acetic acid. Rooted plantlets were directly transferred into pots and grown under field conditions. Seed setting
invariably occurred in all plants.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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The effect of the interaction of genotype and culture medium on the initiation of callus from immature embryos and subsequent plant regeneration was investigated in eight hexaploid wheat lines. Intervarietal differences in culture response and interaction of the genotype with coconut milk are reported. The relative contributions of media and genotype effects to culture performance are assessed. The observation that primordia and shoot development was promoted by coconut milk in some lines and inhibited in others is particularly significant given that coconut milk is widely used to try to improve culture response. This report shows that this effect is dependent on the genotype of the tissues in culture. 相似文献
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Summary The present state of genetic control of in vitro responses of wheat and of the prospeets of its improvement and practical use are presented. The genetic factors affecting
different stages of callus induction and of organogenesis in immature embryos are discussed. The dominant genotypie role in
the in vitro processes and the cytoplasmic influence on the latter are shown. Genotype × environment and nucleus × cytoplasm interactions
were observed. Some unsolved problems needing further investigations of cereals at the tissue culture level are pointed out. 相似文献
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The effect of microelements on the induction of embryogenic callus from epiblast and scutellum of wheat (Triticum aestivum L.) embryos was studied by the sequential omission of each of the microelements from Murashige & Skoog medium. Omission of iron caused a marked decrease in yield and poor shoot formation from embryogenic callus. The yield of embryogenic callus on medium without added manganese was also reduced. Omission of boron, copper-cobalt, iodine, and molybdenum had little effect on the induction of embryogenic epiblast callus. By contrast there was a marked increase in the formation of white structures on the medium without any microelements or, specifically without addition of zinc. Since the formation of typical embryoids of wheat is associated with the formation of white structures, our result highlights the importance of certain microelements on somatic embryogenesis of wheat.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- MS medium
Murashige & Skoog medium 相似文献