共查询到20条相似文献,搜索用时 31 毫秒
1.
《The International journal of biochemistry》1994,26(10-11):1305-1312
AMP-deaminase was purified to homogeneity from white skeletal muscle of control (resting) and exercised (1 min burst swimming) rainbow trout, Oncorhynchus mykiss. The enzyme showed a subunit molecular weight of 71,600 ± 550 kD, a Km AMP of 1.6–1.8 mM at pH 7, and was affected by allosteric inhibitors (GTP, IMP) amd activators (ADP, ATP). AMP-deaminase was inhibited by MgSO4 but activated by low concentrations of NaCl and KCl (100–150 mM); higher KCl was inhibitory. Exercise resulted in a stable modification of some properties (possibly via reversible phosphorylation); I50 values for IMP decreased by 65% and activation energies (from Arrhenius plots) changed significantly. Other properties were affected by assay pH: Km AMP decreased by 50% and Ka, ADP decreased by 70% when pH was lowered from pH 7.3 (typical of resting muscle) to pH 6.6 (muscle pH after exhaustive exercise). The data suggest that a stable modification of AMP-deaminase during exercise, coupled with effects of reduced cytosolic pH, could enhance enzyme function in the rapid conversion of AMP to IMP in working fish muscle. 相似文献
2.
Wang X Wang X Yin M Xiao Z Ma C Lin Z Wang PG Xu P 《Applied microbiology and biotechnology》2007,76(2):321-328
Attempts were made with success to develop a two-step biocatalytic process for uridine 5′-monophosphate (UMP) production from
orotic acid by Corynebacterium ammoniagenes ATCC 6872: the strain was first cultivated in a high salt mineral medium, and then cells were harvested and used as the catalyst
in the UMP production reaction. Effects of cultivation and reaction conditions on UMP production were investigated. The cells
exhibited the highest biocatalytic ability when cultivated in a medium containing corn steep liquor at pH 7.0 for 15 h in
the exponential phase of growth. To optimize the reaction, both “one-factor-at-a-time” method and statistical method were
performed. By “one-factor-at-a-time” optimization, orotic acid, glucose, phosphate ion (equimolar KH2PO4 and K2HPO4), MgCl2, Triton X-100 were shown to be the optimum components for the biocatalytic reaction. Phosphate ion and C. ammoniagenes cell were furthermore demonstrated as the most important main effects on UMP production by Plackett–Burman design, indicating
that 5-phosphoribosyl-1-pyrophosphate (PRPP) synthesis was the rate-limiting step for pyrimidine nucleotides production. Optimization
by a central composition design (CCD) was then performed, and up to 32 mM (10.4 g l−1) UMP was accumulated in 24 h from 38.5 mM (6 g l−1) orotic acid. The yield was threefold higher than the original UMP yield before optimization. 相似文献
3.
Ester Zoref-Shani Asher Shainberg Oded Sperling 《Biochimica et Biophysica Acta (BBA)/General Subjects》1987,926(3)
The pathways of AMP degradation and the metabolic fate of adenosine were studied in cultured myotubes under physiological conditions and during artificially induced enhanced degradation of ATP. The metabolic pathways were gauged by tracing the flow of radioactivity from ATP, prelabelled by incubation of the cultures with [14C]adenine, into the various purine derivatives. The fractional flow from AMP to inosine through adenosine was estimated by the use of the adenosine deaminase (EC 3.5.4.4) inhibitors, coformycin and 2′-deoxycoformycin. The activities of the enzymes involved with AMP and adenosine metabolism were determined flow of label from ATP to diffusible bases and nucleosides, most of which are effluxed to the incubation medium. This catabolic flow is mediated almost exclusively by the activity of AMP deaminase (EC 3.5.4.6), rather than by AMP 5′-nucleotidase (EC 3.1.3.5), reflecting the markedly higher Vmax/Km ratio for the deaminase. Enhancement of ATP degradation by inhibition of glycolysis or by combined inhibition of glycolysis and of electron transport resulted in a markedly greater flux of label from adenine nucleotides to nucleosides and bases, but did not alter significantly the ratio between AMP deamination and AMP dephosphorylation, which remained around 19:1. Combined inhibition of glycolysis and of electron transport resulted, in addition, in accumulation of label in IMP, reaching about 20% of total AMP degraded. In the intact myotubes at low adenosine concentration, the anabolic activity of adenosine kinase was at least 4.9-fold the catabolic activity of adenosine deaminase, in accord with the markedly higher Vmax/Km ratio of the kinase for adenosine. The results indicate the operation in the myotube cultures, under various rates of ATP degradation, of the AMP to IMP limb of the purine nucleotide cycle. On the other hand, the formation of purine bases and nucleosides, representing the majority of degraded ATP, indicates inefficient activity of the IMP to AMP limb of the cycle, as well as inefficient salvage of hypoxanthine under these conditions. 相似文献
4.
《Journal of Fermentation and Bioengineering》1989,67(2):102-106
Culture conditions for the extracellular production of menaquinone (MK) by a mutant strain, K3-15, of Flavobacterium sp. 238-7 were investigated. A detergent-supplemented culture medium consisted of 60 g of glycerol, 23 g of peptone, 3 g of yeast extract, 7 g of K2HPO4, 5 g of NaCl, 0.8 g of MgSO4 · 7H2O, and 0.5 g of Rikanon UA 5012 in 1 l of tap water, pH 7.0, was constructed. Amounts of MK-4, MK-6, and total MK in 1 l of the medium were 101 mg, 39 mg, and 140 mg, respectively, after 7 d of cultivation at 28°C. Further studies with some additives showed that the addition of cedar wood oil increased the productivity of MK, especially MK-4. In the presence of 0.1% Rikanon UA 5012 and 0.1% cedar wood oil, mutant strain K3-15 produced 155 mg/l intracellularly and 105 mg/l extracellularly) of MK-4 and 27 mg/l (16 mg/l intracellularly and 11 mg/l extracellularly) of MK-6, and the total amount of MK reached 182 mg/l. 相似文献
5.
《Phytochemistry》1986,25(10):2267-2270
5′-Nucleotidase from pigeonpea nodules has been resolved into two forms, N-I and N-II, having M,s of 52 000 and 119 000, respectively. Both forms had pH optima in the acidic range (between pH 5.2 and 5.7) with either CMP, GMP, XMP, IMP or AMP as the substrate. Up to pH 6.6, both forms showed higher activity with CMP followed by GMP, XMP, IMP and AMP, respectively. However, the activity changed with pH in the alkaline range making the enzyme relatively more active with purine nucleotides. Neither of the forms had a requirement for any of the metal ions tested. Fe3+ inhibited the enzyme activity; the inhibition at 5, 10 and 15 mM concentrations being 11, 43 and 47%, respectively with N-I and 14,47 and 52%, respectively with N-II. Km values for AMP, IMP, GMP, CMP and XMP were 0.10, 0.18, 0.40, 0.40 and 0.77 mM, respectively with N-I and 0.12, 0.20, 0.40, 0.40 and 0.99 mM, respectively with N-II. The enzyme was inhibited non-competitively by adenosine and inosine; Ki values being 1.78, 0.25 and 0.30; 3.50, 2.12 and 0.75 mM, respectively with AMP, IMP and XMP as the substrate. 相似文献
6.
《Journal of Fermentation and Bioengineering》1992,73(5):396-402
A real-time, on-line extended Kalman filter was used to describe and monitor the growth of Escherichia coli on glycerol. The growth of E. coli showed an inhibition kinetics with μmax=0.806/h, KS=0.68 g/l and Ki=87.4 g/l. As a feeding strategy, the conventional DO-stat with a DDC-PID control method, in which the dissolved oxygen concentration is maintained at a desired level by varying the substrate feedrate, was employed. The Kalman filter was based on an unstructured mathematical model and on-line measured data. The mathematical model comprised of mass balances of the biomass and substrate as well as kinetic and stoichiometric data which were measured prior to the process. For biomass concentration up to 50 g dry weight/l, the estimation of the process was rather accurate. At higher biomass concentration, product formation, indicated by an intense brown coloring of the fermentation broth, occured. Since the effect of this product on biomass production was not included in the mathematical model, the estimated data diverged from the experimental data at biomass concentrations greater than 50 g dry weight/l. 相似文献
7.
(R)-2-Phenylpropanoic acid was synthesized from the racemic acid through an isomerization reaction involving resting cells of Nocardia diaphanozonaria JCM3208. The isomerization activity of the cells was enhanced 25-fold by adding 5.5 mM racemic 2-phenylpropanoic acid to the culture medium. When 5 mM racemic 2-phenylpropanoic acid was included in the reaction mixture (4 ml) containing resting cells (100 mg dry cell wt) in 25 mM K2HPO4/KH2PO4 buffer (pH 7.0) at 30 °C for 8 h, 4.56 mM (R)-2-phenylpropanoic acid (95.8% e.e.) was formed with a 91% molar conversion yield. 相似文献
8.
《The International journal of biochemistry》1993,25(8):1203-1207
- 1.1. Low Km 5' nucleotidase purified from human seminal plasma has been used in this study to investigate the response of the enzyme to adenine nucleoside di- and triphosphates in the presence of AMP and IMP as substrates.
- 2.2. In the presence of AMP, the addition of 0.5 mM ATP to the enzyme Mg-free results into the highest Vmax/Km ratio value and other experimental combinations of effectors tested cause variation of the kinetic parameters of the enzyme, indicating a control of AMP dephosphorylation by adenine nucleotides.
- 3.3. In the presence of IMP, ATP and ADP activate the enzyme but the response to various experimental combinations of effectors shows no significant difference in the kinetic properties of the enzyme, indicating a different control of the dephosphorylation of IMP.
9.
Studies on purine transport and on purine content in vacuoles isolated from Saccharomyces cerevisiae
Maria Nagy 《生物化学与生物物理学报:生物膜》1979,558(2):221-232
The transport of purine derivatives into vacuoles isolated from Saccharomyces cerevisiae was studied. Vacuoles which conserved their ability to take up purine compounds were prepared by a modification of the method of polybase-induced lysis of spheroplasts.Guanosine > inosine = hypoxanthine > adenosine were taken up with decreasing initial velocities, respectively; adenine was not transported.Guanosine and adenosine transporting systems were saturable, with apparent Km values 0.63 mM and 0.15 mM respectively, while uptake rates of inosine and of hypoxanthine were linear functions of their concentrations.Adenosine transport in vacuoles appeared strongly dependent on the growth phase of the cell culture.The system transporting adenosine was further characterized by its pH dependency optimum of 7.1 and its sensitivity to inhibition by .In the absence of adenosine in the external medium, [14C]adenosine did not flow out from preloaded vacuoles. However, in the presence of external adenosine, a very rapid efflux of radioactivity was observed, indicating an exchange mechanism for the observed adenosine transport in the vacuoles.In isolated vacuoles the only purine derivative accumulated was found to be . 相似文献
10.
耐盐性毒死蜱降解菌HY-1 的产酶培养基及发酵条件优化 总被引:1,自引:0,他引:1
为了明确生化处理和微生物降解的关系,通过增加耐盐菌的比例可以提高农药废水生化处理效果。从农药厂废水中分离到1株耐盐性毒死蜱降解菌——蜡状芽孢杆菌(Bacillus cereus HY-1),以从该菌中提取到的降解酶比活力为指标,进行产酶培养基和发酵条件的优化研究。通过单一因素试验和正交试验,对细菌HY-1的产酸培养基和发酵条件进行了优化。运用SPSS软件进行结果分析,所获优化培养基配方为:葡萄糖6.0 g/L,胰蛋白胨2.2 g/L,K2HPO4 2.0 g/L,KH2PO4 0.2 g/L,MgSO4.7H2O 0.1 g/L,NaCl 0.1 g/L和微量元素溶液2 mL/L。得到菌株发酵培养的最佳优化条件为:种子液培养时间为16 h,发酵培养时间为18 h,接种量为1%(V/V),发酵培养基初始pH值为7.0。氯化钠浓度为0?30 g/L时降解酶比活力不受影响,这是已报道的耐盐性最强的一株毒死蜱降解菌。 相似文献
11.
Kenneth B. Storey 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1985,155(3):339-345
Summary Pyruvate kinases from flight muscle and fat body of the cockroach,Periplaneta americana, were purified to homogeneity. The two tissues contained different forms of the enzyme which were separable by starch gel electrophoresis and isoelectric focusing (pI=5.75 for flight muscle and 6.15 for fat body). Both enzymes had molecular weights of 235,000±20,000.Flight muscle pyruvate kinase displayed Michaelis-Menten kinetics with respect to both ADP and P-enolpyruvate withK
m values of 0.27 and 0.04 mM, respectively.K
m for Mg2+ was 0.60 mM andK
a for K+ was 15 mM. The enzyme was weakly inhibitied by four compounds, ATP, arginine-P,l-alanine and citrate with apparentK
i values of 3.5, 15, 20 and 24 mM, respectively. Competitive inhibition by 3 mM ATP or 10 mM arginine-P raised theK
m for P-enolpyruvate to 0.067 or 0.057 mM. Fructose-1,6-P2 did not activate the enzyme but reversed inhibitions by ATP and arginine-P.Fat body pyruvate kinase showed sigmoidal kinetics with respect to P-enolpyruvate with S0.5=0.32 mM andn
H=1.43.K
m values for ADP and Mg2+ were 0.30 and 0.80 mM, respectively with aK
a for K+ of 10 mM. ATP andl-alanine were inhibitors of the enzyme; 2 mM ATP raised S0.5 for P-enolpyruvate to 0.48 mM while 3 mMl-alanine increased S0.5 to 0.84 mM. Neither citrate nor arginine-P inhibited the enzyme but citrate affected the enzyme by reversingl-alanine inhibition. Fat body pyruvate kinase was strongly activated by fructose-1,6-P2 with an apparentK
a of 1.5 M. Fructose-1,6-P2 at 0.1 mM reduced S0.5 for P-enolpyruvate to 0.05 mM andn
H to 1.0.Flight muscle and fat body pyruvate kinases from the cockroach show properties analogous to those of the muscle and liver forms of mammalian pyruvate kinase. Fat body pyruvate kinase is suited for on-off function in a tissue with a gluconeogenic capacity. Strong allosteric control with a feed-forward activation by fructose-1,6-P2 is key to coordinating enzyme function with glycolytic rate. The function of flight muscle pyruvate kinase in energy production during flight is aided by a lowK
m for P-enolpyruvate, weak inhibitor effects by high energy phosphates and deinhibition of these effects by fructose-1,6-P2. 相似文献
12.
Kimon J. Angelides 《Journal of neurochemistry》1980,35(4):949-962
Highly purified “heavy” synaptic vesicles were isolated from rat heart by differential centrifugation. Because of the high intravesicular concentrations of proteins, catecholamine, and ATP, resealed vesicle ghosts were prepared and used to study the detailed kinetics of catecholamine transport. ATP stimulated the uptake of /-norepinephrine and was saturable with a Km for l-norepinephrine at 3.3 μM and 1.8 mM for ATP. The ghosts also accumulated 5-hydroxytryptamine and l-epinephrine via an ATP-dependent mechanism. Uptake was stereospecific for the l-form. A functional catecholamine transporter could be solubilized by the detergent octyl-glucoside and incorporated into phospholipid vesicles, which, after detergent removal, generated proteoliposomes that accumulated l-norepinephrine. Reserpine- and l-propranolol-sensitive accumulation against a concentration gradient is achieved by artificially creating a pH gradient across the membrane, and lends further support to the idea that at least the initial phase of catecholamine transport is driven by the trans-membrane pH gradient created by the proton-translocating ATPase. 相似文献
13.
The kinetic properties of pyruvate kinase (ATP:pyruvate-phosphotransferase, EC 2.7.1.40) from Streptococcus lactis have been investigated. Positive homotropic kinetics were observed with phosphoenolpyruvate and adenosine 5′-diphosphate, resulting in a sigmoid relationship between reaction velocity and substrate concentrations. This relationship was abolished with an excess of the heterotropic effector fructose-1,6-diphosphate, giving a typical Michaelis-Menten relationship. Increasing the concentration of fructose-1,6-diphosphate increased the apparent Vmax values and decreased the Km values for both substrates. Catalysis by pyruvate kinase proceeded optimally at pH 6.9 to 7.5 and was markedly inhibited by inorganic phosphate and sulfate ions. Under certain conditions adenosine 5′-triphosphate also caused inhibition. The Km values for phosphoenolpyruvate and adenosine 5′-diphosphate in the presence of 2 mM fructose-1,6-diphosphate were 0.17 mM and 1 mM, respectively. The concentration of fructose-1,6-diphosphate giving one-half maximal velocity with 2 mM phosphoenolpyruvate and 5 mM adenosine 5′-diphosphate was 0.07 mM. The intracellular concentrations of these metabolites (0.8 mM phosphoenolpyruvate, 2.4 mM adenosine 5′-diphosphate, and 18 mM fructose-1,6-diphosphate) suggest that the pyruvate kinase in S. lactis approaches maximal activity in exponentially growing cells. The role of pyruvate kinase in the regulation of the glycolytic pathway in lactic streptococci is discussed. 相似文献
14.
Adenine nucleotide metabolism and nucleoside transport in human erythrocytes under ATP depletion conditions 总被引:1,自引:0,他引:1
The adenine nucleotides of human red cells were labeled by incubation of the cells with [3H]adenosine. Then, the cells were incubated in Tris-saline with various supplements that cause the loss of cellular ATP, and the degradation products were quantitated as a function of time of incubation at 37 degrees C. Incubation of the cells with 2.5 or 5 mM iodoacetate, iodoacetamide or 1 mM HCHO in combination with 5 mM KF and 50 mM deoxyglucose, 50 mM D-glucose or 10 mM inosine was most efficient in depleting the cells of ATP (100% in 0.5-1 h) without causing cell lysis. In iodoacetate- and iodoacetamide-treated cells practically all catabolism of ATP occurred via ADP----AMP----IMP----inosine----hypoxanthine with hypoxanthine accumulating in the medium. In HCHO-treated cells and in cells incubated in Tris-saline or in Tris-saline with deoxyglucose with and without KF, a substantial proportion of ATP (up to 50%) was catabolized via ADP----AMP----adenosine----inosine----hypoxanthine. Under all conditions, AMP deamination and IMP and AMP hydrolysis were rate-limiting reactions. IMP degradation was more rapid in iodoacetamide- and HCHO-treated than in iodoacetate-treated red cells. It was also more rapid in fresh than in outdated red cells, and it was inhibited by Pi. Treatment with iodoacetamide and HCHO under ATP-depletion conditions resulted in a 60-80% inhibition of uridine transport by the cells. Treatment with iodoacetate or deoxyglucose plus KF had only minor effects on nucleoside transport; thus, cells treated in this manner might be useful for studying the transport of adenosine and deoxyadenosine under conditions were their phosphorylation is prevented. 相似文献
15.
Small intact frog skeletal muscles were exposed to radioactively labeled adenosine 3′,5′-cyclic monophosphate (cAMP) during incubation in frog Ringer's solution buffered with Tris (RT). The fate of the nucleotide was followed by measuring the products in the incubation media. Paper chromatography was used for the separation and identification of these products; the amounts were measured using liquid scintillation spectrometry. It was found that cAMP was degraded to AMP, which was then converted to IMP and, to some extent, inosine. The degradation of cAMP to AMP was markedly inhibited by theophylline (10 mM) suggesting the presence of cAMP phosphodiesterase activity at the muscle surface. Kinetic studies of enzyme activity in situ revealed two apparent Km values: 0.33 μm and 55 μm. Insulin (0.3 unit/ml) increased the phosphodiesterase activity at concentrations of cAMP ranging from 2 to 17 μm. The possible roles of the surface phosphodiesterase were discussed. 相似文献
16.
Quillaguamán J Doan-Van T Guzmán H Guzmán D Martín J Everest A Hatti-Kaul R 《Applied microbiology and biotechnology》2008,78(2):227-232
High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l−1). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the
final cell density. Effect of initial concentrations of NH4Cl and K2HPO4 on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch fermentation system. The best conditions for PHB production by H. boliviensis were attained using 0.4% (w/v) NH4Cl and 0.22% (w/v) K2HPO4 and adding MSG intermittently to the fermentor. Poly(3-hydroxybutyrate) content and CDW reached 90 wt.% and 23 g l−1, respectively, after 18 h of cultivation. In order to increase CDW and PHB content, MSG, NH4Cl, and K2HPO4 were initially fed to the fermentor to maintain their concentrations at 2%, 0.4%, and 0.22% (w/v), respectively, and subsequently their feed was suppressed. This resulted in a CDW of 44 g l−1, PHB content of 81 wt.%, and PHB volumetric productivity of 1.1 g l−1 h−1. 相似文献
17.
Bingkun Yan Qingbao Ding Ling Ou Zhi Zou 《World journal of microbiology & biotechnology》2014,30(3):1123-1128
A process of glucose-6-phosphate (G-6-P) production coupled with an adenosine triphosphate (ATP) regeneration system was constructed that utilized acetyl phosphate (ACP) via acetate kinase (ACKase). The genes glk and ack from Escherichia coli K12 were amplified and cloned into pET-28a(+), then transformed into E. coli BL21 (DE3) and the recombinant strains were named pGLK and pACK respectively. Glucokinase (glkase) in pGLK and ACKase in pACK were both overexpressed in soluble form. G-6-P was efficiently produced from glucose and ACP using a very small amount of ATP. The conversion yield was greater than 97 % when the reaction solution containing 10 mM glucose, 20 mM ACP-Na2, 0.5 mM ATP, 5 mM Mg2+, 50 mM potassium phosphate buffer (pH 7.0), 4.856 U glkase and 3.632 U ACKase were put into 37 °C water bath for 1 h. 相似文献
18.
Indigoidine is a bacterial natural product with antioxidant and antimicrobial activities. Its bright blue color resembles the industrial dye indigo, thus representing a new natural blue dye that may find uses in industry. In our previous study, an indigoidine synthetase Sc-IndC and an associated helper protein Sc-IndB were identified from Streptomyces chromofuscus ATCC 49982 and successfully expressed in Escherichia coli BAP1 to produce the blue pigment at 3.93 g/l. To further improve the production of indigoidine, in this work, the direct biosynthetic precursor l-glutamine was fed into the fermentation broth of the engineered E. coli strain harboring Sc-IndC and Sc-IndB. The highest titer of indigoidine reached 8.81 ± 0.21 g/l at 1.46 g/l l-glutamine. Given the relatively high price of l-glutamine, a metabolic engineering technique was used to directly enhance the in situ supply of this precursor. A glutamine synthetase gene (glnA) was amplified from E. coli and co-expressed with Sc-indC and Sc-indB in E. coli BAP1, leading to the production of indigoidine at 5.75 ± 0.09 g/l. Because a nitrogen source is required for amino acid biosynthesis, we then tested the effect of different nitrogen-containing salts on the supply of l-glutamine and subsequent indigoidine production. Among the four tested salts including (NH4)2SO4, NH4Cl, (NH4)2HPO4 and KNO3, (NH4)2HPO4 showed the best effect on improving the titer of indigoidine. Different concentrations of (NH4)2HPO4 were added to the fermentation broths of E. coli BAP1/Sc-IndC+Sc-IndB+GlnA, and the titer reached the highest (7.08 ± 0.11 g/l) at 2.5 mM (NH4)2HPO4. This work provides two efficient methods for the production of this promising blue pigment in E. coli. 相似文献
19.
Da-Hye Jung Wonji Choi Kwon-Young Choi Eunok Jung Hyungdon Yun Romas J. Kazlauskas Byung-Gee Kim 《Applied microbiology and biotechnology》2013,97(4):1501-1511
Phenolic acid decarboxylase (PAD) catalyzes the non-oxidative decarboxylation of p-coumaric acid (pCA) to p-hydroxystyrene (pHS). PAD from Bacillus amyloliquefaciens (BAPAD), which showed k cat/K m value for pCA (9.3?×?103?mM?1?s?1), was found as the most active one using the “Subgrouping Automata” program and by comparing enzyme activity. However, the production of pHS of recombinant Escherichia coli harboring BAPAD showed only a 22.7 % conversion yield due to product inhibition. Based on the partition coefficient of pHS and biocompatibility of the cell, 1-octanol was selected for the biphasic reaction. The conversion yield increased up to 98.0 % and 0.83 g/h/g DCW productivity was achieved at 100 mM pCA using equal volume of 1-octanol as an organic solvent. In the optimized biphasic reactor, using a three volume ratio of 1-octanol to phosphate buffer phase (50 mM, pH 7.0), the recombinant E. coli produced pHS with a 88.7 % conversion yield and 1.34 g/h/g DCW productivity at 300 mM pCA. 相似文献
20.
Use of glycerol for producing 1,3-dihydroxyacetone by Gluconobacter oxydans in an airlift bioreactor
1,3-Dihydroxyacetone can be produced by biotransformation of glycerol with glycerol dehydrogenase from Gluconobacter oxydans cells. Firstly, improvement the activity of glycerol dehydrogenase was carried out by medium optimization. The optimal medium for cell cultivation was composed of 5.6 g/l yeast extract, 4.7 g/l glycerol, 42.1 g/l mannitol, 0.5 g/l K2HPO4, 0.5 g/l KH2PO4, 0.1 g/l MgSO4·7H2O, and 2.0 g/l CaCO3 with the initial pH of 4.9. Secondly, an internal loop airlift bioreactor was applied for DHA production from glycerol by resting cells of G. oxydans ZJB09113. Furthermore, the effects of pH, aeration rate and cell content on DHA production and glycerol feeding strategy were investigated. 156.3 ± 7.8 g/l of maximal DHA concentration with 89.8 ± 2.4% of conversion rate of glycerol to DHA was achieved after 72 h of biotransformation using 10 g/l resting cells at 30 °C, pH 5.0 and 1.5 vvm of aeration rate. 相似文献