Experiments on Nitrogen and Phosphorus Release by Chironomus ex gr. plumosus from the Sediments of Lake Balaton,Hungary

Sediment-water systems of Lake Balaton mud and tap water were constructed in plexiglass tubes and boxes for evaluation of changes in the nitrogen and phosphorus contents of overlying water as influenced by chironomid density and light conditions. Nitrogen flux from sediment was greatly influenced by denitrification process. The amount of TN released by chironomids amounted to about half of the yearly nitrogen fixation rate in Lake Balaton. Omnivorous chironomids increased the phosphorus content of the overlying water. The rate of release was density dependent. TP release rates ranged between 1–17 mg m⁻² day ⁻¹ over a range of densities 500–20,000 larvae m⁻². It became evident that aerobic sediment cores can be an important nutrient source in lakes where chironomids inhabit them at densities above 1,000 larvae m⁻².     

Determination of the Limiting Plant Nutrient in the Water of Lake Balaton by Algal Assay Procedure

Algal assays were performed on water samples taken from different sites in Lake Balaton. Selenastrum capricornutum was used in the test to determine the primarily limiting plant nutrient. The results of supplementary nutrient additions to the bottle tests were evaluated by cell counts. The tests have indicated phosphorus as growth rate limiting, and in two cases as biomass limiting plant nutrient, but periodic occurrences of nitrogen limitation cannot be excluded.     

杭州西湖北里湖沉积物氮磷内源静态释放的季节变化及通量估算

通过采集北里湖不同季节的柱状芯样,在实验室静态模拟沉积物氨氮(NH+4-N)和可溶解性磷酸盐(PO3-4-P)的释放,同时研究了沉积物间隙水中NH+4-N及PO3-4-P的垂直分布特征.结果表明,沉积物间隙水NH+4-N随深度的增加有上升的趋势,PO3-4-P随深度的增加呈先升后降的趋势.氮、磷营养盐在沉积物—水界面均存在浓度梯度,表明存在自间隙水向上覆水扩散的趋势.沉积物NH+4-N在春季、夏季、秋季、冬季的释放速率分别为0.074 mg·m-2· d-1、0.340mg· m-2· d-1、0.087 mg· m-2· d-1、0.0004 mg·m-2·d-1,pO3-4-P的释放速率则分别为0.340 mg·m-2·d-1、0.518 mg·m-2·d-1、0.094 mg·m-2·d-1、-0.037 mg· m-2·d-1.不同采样点表现出明显的季节和空间差异性,释放速率表现为夏季＞春季、秋季＞冬季.根据静态模拟出的不同季节下内源氮、磷释放速率计算,全湖内源氮、磷营养盐的贡献分别为0.0037、0.0057t/a.该研究可为北里湖富营养化及内源污染的治理提供基础数据.     

Production and chlorophyll concentration of epipelic and epilithic algae in fertilized and nonfertilized subarctic lakes

The production and chlorophyll concentration of epipelic and epilithic algae was measured during four years (1972–1975) in two shallow, Swedish subarctic lakes. One lake (Lake Hymenjaure) was fertilized with phosphorus or a combination of phosphorus and nitrogen while the other (Lake Stugsjön) served as a reference. The benthic algae in both lakes were dominated by Cyanophyceae of the same species during the whole investigation. The chlorophyll concentration of epipelic and epilithic algae was 100 and 20 mg·m^–2 respectively and fairly constant during the season. In 1974–1975 there was a significant increase in chlorophyll concentration of the benthic algae in Lake Hymenjaure, probably as a response to the poorer light climate in the lake due to a large phytoplankton development. The annual benthic production was 3.4–7.2 gC·m^–2 and it was not enhanced by the fertilization. Compared to the other primary producers (phytoplankton and macrophytes) the benthic algae constituted 70–83% of the total production in Lake Stugsjön. In Lake Hymenjaure, however, the importance of the benthic algae decreased from 50 to 22% of the total due to the great increase in phytoplankton production induced by the lake fertilization.     

Bioassays with Stigeoclonium Kütz. (Chlorophyceae) to identify nitrogen and phosphorus limitations

Eleven strains of the filamentous algae genus Stigeoclonium Kütz. (Chlorophyceae) were tested as bioassay organisms to identify nitrogen and phosphorus limitations to growth in a variety of waters. The assay results were related to the nutrient status of the waters during the year. Optimal growth was obtained at an inorganic phosphorus concentration of 0.65 mg P 1^?1 and an inorganic nitrogen concentration of 3.75 mg N 1^?1. The algal growth potentials were generally higher in November and February than in August. In summer, nitrogen became important as a limiting nutrient. When ratios of inorganic nitrogen to inorganic phosphorus (N/P) were above 6.2, phosphorus was primarily limiting and at lower N/P ratios nitrogen became the important limiting factor. Stigeoclonium proved to be a suitable bioassay organism to identify nutrient limitation in freshwaters.     

城市化背景下白洋淀入湖营养盐负荷模拟研究

过量的营养盐输入导致白洋淀富营养化程度较高。雄安新区建设伴随着快速城镇化进程,将可能进一步增加入湖营养盐负荷。为摸清新区建设背景下白洋淀入湖营养盐负荷的变化趋势及削减量,基于入湖营养盐负荷模型计算了历史不同时期（1995-2015年）白洋淀入湖营养盐盐负荷,预测了土地利用、农业管理和污水处理系统变化下入湖营养盐负荷的变化趋势。结果表明,白洋淀历史时期入湖总氮和总磷年平均负荷分别为2018 t和313 t,主要来源分别是耕地和畜禽粪便排放。就土地利用变化而言,以2010年为基准,2050年白洋淀入湖总氮和总磷负荷在建设用地快速增长（RAP）情景下增长率最高,分别增长了56%和60%,主要原因在于城镇人口增加,从而增加了畜禽需求量和生活污水排放。就农业管理而言,削减化肥使用有利于降低入湖总氮负荷,控制畜禽粪便排放更有利于入湖总磷负荷的降低。2050年,生活污水将成为白洋淀入湖营养盐的重要来源,因此,在制定未来社会经济发展路径时,应重点提高农村地区生活污水收集率和处理效率。PCLake模型对不同入湖负荷下湖泊营养状态演变的模拟发现,削减入湖总磷负荷是白洋淀水质恢复的关键。白洋淀水质达到III类和IV类标准时,入湖总磷负荷阈值分别为2.1和2.6 mg P m^-2 d^-1。在建设用地快速增长情景下,若使白洋淀水质达到III类标准,入湖总磷负荷应减少131 t。     

Lake restoration using a reed swamp to remove nutrients from non-point sources

The reed swamp adjacent to Lake Glumsø was partly separated from the lake by a dam and the inflow to the reed swamp controlled by pumping from the tributary. An investigation of the nutrient balances for the reed swamp showed an average daily denitrification rate of 252 mg/m2 from August 1988 to March 1989. Mineralization of the peat took place simultaneously, in sufficient amounts to supply carbon for the denitrification process. Ammonia and phosphorus were released in the ratio 7:1, corresponding to 111 mg N and 16 mg P/m²/ day. Most of the ammonia was nitrified and denitrified (included in the above mentioned denitrification rate). The net release of phosphorus implies that the method only can be applied satisfactorily in situations where nitrogen is the limiting nutrient. This is the case for Lake Glumso during the summer period, but not during the spring.This paper was presented at the INTECOL IV International Wetlands Conference in Columbus, Ohio, 1992, as part of a session organized by Prof. S. E. Jørgensen and sponsored by the International Lake Environment Committee.Corresponding Editor: Prof K. R. Reddy     

Control of phosphorus loading and flushing as restoration methods for Lake Veluwe,The Netherlands

As a result of high nutrient loading Lake Veluwe suffered from an almost permanent bloom of the blue-green algaOscillatoria agardhii Gomont. In 1979, the phosphorus loading of the lake was reduced from approx. 3 to 1 g P.m^–2.a^–1. Moreover, since then the lake has been flushed during winter periods with water low in phosphorus. This measure aimed primarily at interrupting the continuous algal bloom. The results of these measures show a sharp decline of total-phosphorus values from 0.40–0.60 mg P.l^–1 (before 1980) to 0.10–0.20 mg P.l^–1 (after 1980). Summer values for chlorophylla dropped from 200–400 mg.m^–3 to 50–150 mg.m^–3.The increase in transparency of the lake water was relatively small, from summer values of 15–25 cm before the implementation of the measures to 25–45 cm afterwards. The disappointing transparency values may be explained by the decreasing chlorophylla and phosphorus content of the algae per unit biovolume. Blue-green algae are gradually loosing ground. In the summer of 1985 green algae and diatoms dominated the phytoplankton for the first time since almost 20 years. To achieve the ultimate water quality objectives (transparency values of more than 100 cm in summer), the phosphorus loading has to be reduced further.     

Interactions between sediment and water in a shallow and hypertrophic lake: a study on phytoplankton collapses in Lake Søbygård,Denmark

Short-term changes in phytoplankton and zooplankton biomass have occurred 1–3 times every summer for the past 5 years in the shallow and hypertrophic Lake Søbygård, Denmark. These changes markedly affected lake water characteristics as well as the sediment/water interaction. Thus during a collapse of the phytoplankton biomass in 1985, lasting for about 2 weeks, the lake water became almost anoxic, followed by rapid increase in nitrogen and phosphorus at rates of 100–400 mg N M^–2 day^–1 and 100–200 mg P m^–1 day^–1. Average external loading during this period was about 350 mg N m^–2 day^–1 and 5 mg P m^–2 day^–1, respectively.Due to high phytoplankton biomass and subsequently a high sedimentation and recycling of nutrients, gross release rates of phosphorus and nitrogen were several times higher than net release rates. The net summer sediment release of phosphorus was usually about 40 mg P m^–2 day^–1, corresponding to a 2–3 fold increase in the net phosphorus release during the collapse. The nitrogen and phosphorus increase during the collapse is considered to be due primarily to a decreased sedimentation because of low algal biomass. The nutrient interactions between sediment and lake water during phytoplankton collapse, therefore, were changed from being dominated by both a large input and a large sedimentation of nutrients to a dominance of only a large input. Nitrogen was derived from both the inlet and sediment, whereas phosphorus was preferentially derived from the sediment. Different temperature levels may be a main reason for the different release rates from year to year.     

Experimental study of the effect of nutrients on phytoplankton of the shallow highly eutrophic Lake Nero

Experimental studies of the effect of phosphorus, nitrogen, and silicon on the phytoplankton of the shallow highly eutrophic Lake Nero have been carried out. It is shown that the enrichment of lake water with nitrogen leads to an increased concentration of chlorophyll a and the growth of the biomass of dominant algae species. Phosphorus limits the abundance of a certain species of algae; the limiting role of silicon in the development of spring phytoplankton has not been experimentaly confirmed.     

Nutrient limitation of the primary production of phytoplankton in Lake Baikal

Nutrient limitation of the primary production of phytoplankton at some stations in southern and central Lake Baikal was studied by nutrient enrichment experiments in August 2002. Chlorophyll (Chl.) a concentrations ranged from 0.7 to 5.8μgl⁻¹. Inorganic nutrient concentrations were low: soluble reactive phosphorus ranged from 0.05 to 0.20μmoll⁻¹, ammonia from 0.21 to 0.41μmoll⁻¹, and nitrite plus nitrate from 0.33 to 0.37μmoll⁻¹. In the five enrichment experiments, phosphate spikes and phosphate plus nitrate spikes always stimulated primary production. Nitrate spikes also stimulated primary production in four of the experiments. Significant differences were detected between the controls and phosphate spikes and between the controls and phosphate plus nitrate spikes. Thus, the first limiting nutrient is thought to be phosphorus, but once phosphorus is supplied to the surface water, the limiting nutrient will quickly shift from phosphorus to nitrogen.     

Phosphorus availability and nitrogenase activity in aquatic blue-green algae

Metabolically active phosphorus-starved cultures of blue-green algae assimilate ³²P rapidly in the light and in the dark. The uptake of phosphorus results in a rapid (within 15 min) stimulation in acetylene reduction by Anabaena cylindrica, A. flosaquae, Anabacnopsis circuiaris and Chlorogloea fritschii, with a response being obtained to less than 5 μg/1 of phosphorus. Uptake of phosphorus also causes a rapid increase in respiration in the dark but not in photo respiration, and the size of the cellular ATP pool and the ¹⁴CO₂ fixation rate both increase more slowly. The metabolism of phosphorus-sufficient cells, which assimilate phosphorus more slowly, shows little response when phosphorus is provided. Excess phosphorus is stored in the vegetative cells of blue-green algae as polyphosphate bodies which may form within 60 min of adding phosphorus to phosphorusstarved cells and which serve as a source of phosphorus for the algae when exogenous phosphorus is limiting. Preliminary results from Scottish waters suggest that urban effluents are important sources of available-phosphorus for algal growth and that the levels entering fresh waters from agricultural land are, per unit volume, lower. In both types of water the levels of available-phosphorus are rather similar to the levels of orthophosphate-phosphorus present. Most detergents tested serve as a source of phosphorus for nitrogen-fixing blue-green algae and cause a rapid stimulation in reduction when added to phosphorus-starved cultures. Of the detergents assayed, the biological types were richest in available phosphorus. The addition of detergents may result in a rapid increase in number of polyphosphate bodies present in the algae. Detergents in general also contain an inhibitor of algal metabolism. Whether a stimu-lation or an inhibition occurs depends on the quantities of detergent added and on whether or not the alga is phosphorus-deficient.     

Some Bag Experiments on Tibbs Run Lake,W.V., A Eutrophic Acidified Lake

Tibbs Run Lake was sampled from December 1979 to December 1980, monthly during the winter and biweekly during the spring, summer, and autumn. Primary production was measured from March 1980 to February 1981, either monthly or biweekly, as indicated above. The mean annual hydrogen ion concentration was equivalent to pH 4.33. This pH was 46% lower (based on H⁺ concentrations) than the 1977–1978 mean. The low pH of the lake is due to inputs of acid precipitation and the low buffering capacity of the watershed. Predictions of further reductions in the lake pH, based on mean annual hydrogen ion concentrations and hydrogen ion retention coefficients (RH) from Shellito (1979) and this study, indicate that the lake pH should reach an equilibrium with the precipitation pH between 1982 and 1985, assuming the precipitation pH continues to average 4.10. The stratified period mean chlorophyll a and total phosphorus concentrations were 22.1 mg/m³ and 19.3 μg/1, respectively. Total phosphorus loading to the lake was 0.233 gP/m²/yr. To examine the effects of neutralization and fertilization with phosphorus an in situ bag enclosure experiment, lasting 16 days, was performed during the summer of 1980. In the bags, phosphorus (20 μg/1 on days 0,4,8,12 as KH₂PO₄) was added alone and in combination with a base addition (IN KOH). The base additions raised the pH to approximately 7.8. Other treatments included base addition alone and a control. The maximum chlorophyll a concentration was found in the phosphorus treatment (∼30 mg/m³) on day 6. The phosphorus plus base treatment exhibited a 10 day lag before reaching a maximum chlorophyll a concentration (∼27 mg/m³) on day 16. The highest production rate was found in the phosphorus plus base treatment on day 12 (∼27 mgC/m³/hr.). A similar bag experiment, lasting 46 days, was performed during the late summer and early autumn of 1980. The treatments were the same as in the first experiment, however, the nitrate-nitrogen depletion in the lake and treatments necessitated the addition of nitrogen (2 mg/1 as NaNO₃) to one of each replicate treatment on day 5. On day 34, the maximum chlorophyll a concentrations were found in the phosphorus plus base nitrogen treatment (∼120 mg/m³) and the phosphorus plus nitrogen treatment (∼38.8 mg/m³). The control plus nitrogen treatment was submerged and possibly contaminated on day 5. All treatments not receiving nitrogen, except for the phosphorus plus base treatment, decreased in the chlorophyll a concentration throughout the experiment. The phosphorus plus base treatment peaked in the chlorophyll a concentration on day 45 (∼28 mg/m³) despite non-detectable levels of nitrate-nitrogen. The maximum primary production rate was found in the phosphorus plus base plus nitrogen treatment (∼32 mgC/m³/hr.) on day 11.     

FACTORS REGULATING THE SPATIAL DISTRIBUTION OF THE FILAMENTOUS ALGA PITHOPHORA OEDOGONIA (CHLOROPHYCEAE) IN AN INDIANA LAKE1

Pithophora oedogonia (Mont.) Wittr. biomass in Surrey Lake, Indiana was greater in the littoral than in the pelagial region. Although mean soluble reactive phosphorus concentrations did not differ between the two areas, nitrate concentrations were almost six times higher in the cove than in the open water. Using laboratory cultures of Pithophora, the half saturation constant (K_s at 20° C relating filament growth to external concentrations of nitrate-nitrogen was determined to be 1.23 mg L^?1 (=88 μM)and for phosphate-phosphorus, 0.1 mg L^?1 (=3.22 μM). These values were used to calculate a NO₃-N/PO₄-P atomic ratio of 27.6. Comparison of this value with NO₃-N/PO₄-P ratios in Surrey Lake showed that nitrogen limiting conditions were prevalent in the open water section of the lake. Alkaline phosphatase and dark ammonia uptake analyses on field collected filaments from the shallow and deep water sections confirmed the hypothesis that nitrate is the major factor limiting growth of Pithophora in Surrey Lake.     

Mathematical modelling as a tool for management in eutrophication control of shallow lakes

The eutrophication model Delwaq-Bloom-Switch is developed to be a functional tool for water management. Therefore it includes nutrients, algal biomass and composition as well as water transparency. A module describing the interaction between water and bottom gives the model the flexibility to deal with measures, such as a decrease of the external phosphorus loading and flushing with water differing in composition from the lake water. This paper focuses on the functional aspects of the model, the results of an application on Lake Veluwe, The Netherlands, and the implications for water management.With one set of coefficients DBS reproduces the most important characteristics of Lake Veluwe for a period of two years before measures (reduction of the external loading and flushing during the winter months) and eight years after the measures. The phosphorus concentration decreased and became growth limiting for algae instead of nitrogen and light. Both in measurements and modelling results, the algal composition changed from blue-green algae dominance to green algae and diatom dominance. Lake Veluwe had a relatively short transient phase after reduction of external loading, because high nitrate concentrations in the flushing water inhibited a long period with high phosphorus releases from the bottom.Model calculations were carried out to investigate the effects of fish stock management and optimization of flushing. Both measures are promising.     

Effects of nitrogen,phosphorus and carbon enrichment on planktonic and periphytic algae in a softwater,oligotrophic lake in Florida,USA

The objective of this study was to investigate nutrient limitation of algal abundance in Anderson-Cue Lake, a softwater clear oligotrophic lake in north-central Florida. Nutrient diffusing clay pots and cylindrical enclosures were used in the field to test effects of different combinations of nitrogen, phosphorus, silica, and carbon on algal standing crop and composition of periphytic and planktonic algae, respectively. Effects of nutrient enrichment on periphytic algae were examined in two studies conducted 31 May – 8 July and 10 June – 15 July 1991. Nutrient effects on planktonic algae were examined in one study from 13 June – 1 July 1991. Planktonic and periphytic algal biovolume was significantly higher (p<0.05) when nitrogen and carbon were added in combination than with treatments without nitrogen, carbon, or nitrogen and carbon. Treatments with nitrogen and carbon combined resulted in lower algal diversity and dominance by coccoid green algae andScenedesmus. Results indicate that carbon and nitrogen can be limiting factors to algal growth in Anderson-Cue Lake and possibly other lakes of similar water quality.     

Variation in the Settling Velocity of Suspended Particulate Matter in Shallow Lakes,with Special Implications for Mass Balance Modelling

Measurements of sedimentation were combined with water samples to calculate settling velocity of suspended particulate matter (SPM) in lakes. The study sites were open water stations and enclosures in Lake Erken (Sweden) and Lake Balaton (Hungary). Settling velocities were found to vary considerably both inside and outside the enclosures. Within sites, the differences between 25th and 75th percentiles of measured settling velocities of SPM were two‐ or three‐fold. Median settling velocities of SPM ranged from around 0.5 m/d in the enclosures of Lake Erken to more than 8 m/d in the open water of Lake Balaton. Special relevance was attributed to flocculation, which is known to be affected by, e.g., SPM concentration and turbulence. Even though not directly measured, the less turbulent environment inside the enclosures was suggested to explain the low settling velocity compared to the open water environment. Settling velocity apparently correlated with water current speed (r² = 0.66; n = 12). Stepwise multiple linear regressions were used to relate the variability in settling velocity of SPM to the variability of possible controlling factors in a number of data subsets. In most cases, one variable describing the total amount of settling material (e.g., SPM) and one variable reflecting the composition of settling material (e.g., total phosphorus) were chosen. The use of suspended solids concentration to predict settling velocity in mass balance models was discussed. It was found that the mean slope between SPM and v_SPM was close to 0.1 (m⁴d^–1 g^–1). (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

On the Mechanism Reducing the Phosphate Concentration in the Water of Lake Balaton

The aim of the investigations was to identify the main process controlling the rather low phosphate concentration in the water of Lake Balaton. Three processes were taken into consideration: a) phosphate uptake by algae, b) coprecipitation with biogenic lime, and c) adsorption on suspended sediment particles. A plexiglass box open at the bottom was placed in the shallow water with its sides extending into the sediment. The water in the box was stirred. When phosphate alone was added to the water in the box, its concentration decreased very rapidly. On the other hand, if NaOCl was also added in order to kill algae and bacteria, the phosphate concentration remained constant. This proves the priority of life processes in phosphate removal. In the next experiment phosphate and EDTA-Na₂ were added simultaneously to the water in the box. The complexing agent prevented biogenic lime formation but did not influence the rapid phosphorus removal. This fact indicates that the main process involved in phosphate control is direct uptake by algae. The same conclusion was obtained in laboratory experiments where labelled phosphate was added to the bottles containing Lake Balaton water. It was demonstrated by the photo-oxidation technique that the phosphate removed from the water was incorporated into the organic particulate matter.     

The Importance of Higher Trophic Level in the Process of Eutrophication in Enclosure

Enclosures open to the sediments and the atmosphere were used to elucidate the response of algae and bacteria to the nutrient enrichment from fish in the mesotrophic area of Lake Balaton. Active transformation between the forms of nitrogen was observed especially in the enclosure with fish. Both nitrogen and phosphorus values were influenced by fish. Changes in nutrient dynamics, composition and biomass of algae and bacterial production were measured. It has been shown that benthivorous fish with a biomass illustrative of the most eutrophic part of the lake were able to generate high bacterial production rate and a strong outburst of blue-greens.     

Development and practical application of limiting values for the phosphate concentration in surface waters in the Netherlands

Summary To counteract eutrophication it is important to possess sufficient information (1) on the relationship between the content of nutrients and the algal biomass, and (2) on the cycling of nutrients in lakes.A comparative study of a large number of surface waters in the Netherlands has demonstrated that it is possible to derive a relationship between the nitrogen and phosphorus concentrations and the upper limit of the chlorophyll concentration, averaged over the summer season.For the authority in charge of water quality it is essential to know how far the phosphorus loading must be reduced to attain the desired phosphate concentration. The results of an extensive study of the nutrient budgets of Lake Wolderwijd-Nuldernauw over the period 1976–1979 demonstrate that in certain cases a relative high phosphate concentration can occur (approx. 0.30 mg/l) in spite of a low external phosphorus loading (approx. 0.7 g P/m² year). Such high concentrations can be explained by the continuous presence of a bloom of blue-green algae and the release of phosphate from the aquatic sediments during the summer. In such lakes it is necessary to take additional measures, such as flushing with water poor in algae and phosphates, or, where necessary, the removal of aquatic sediment rich in phosphate.