共查询到20条相似文献,搜索用时 15 毫秒
1.
Reut Shalgi Jessica A. Hurt Irina Krykbaeva Mikko Taipale Susan Lindquist Christopher B. Burge 《Molecular cell》2013,49(3):439-452
Highlights? Global translation elongation pause at 5′ end of ORFs in severe heat shock ? Modulation of Hsp70 levels/activity affects elongation pausing ? Pausing is associated with hydrophobic N termini ? Hsp70 shows reduced ribosome association and altered interactions in heat stress 相似文献
2.
Susana K Checa Matias D Zurbriggen Fernando C Soncini 《Current opinion in biotechnology》2012,23(5):766-772
Highlights? We describe the use of synthetic biology concepts for bacterial biosensor design. ? Rewiring sensor/transducer modules provide improved bioreporter performance. ? Novel outputs can be embedded into existing regulatory circuits. ? Bioremediation, bioenergy and chemotherapy are future application trends. 相似文献
3.
Audrey Leprince Mark WJ van Passel Vitor AP Martins dos Santos 《Current opinion in biotechnology》2012,23(5):651-658
Highlights? Top-down and bottom-up approaches to genome streamlining. ? Computational support for constructing and refactoring streamlined genomes. ? From genome engineering to metabolic reprogramming. ? Perspectives in applied genome engineering. 相似文献
4.
Highlights? Motivation is the product of past experience and current mesocorticolimbic state ? Novel appetite state transforms a repulsive salt cue into a motivational magnet ? The cue becomes avidly “wanted,” despite knowledge that the salt always tastes disgusting ? This dynamic transformation recruits brain mesocorticolimbic circuitry 相似文献
5.
Fabien Ferrage Kaushik Dutta Estanislao Nistal-Villán Jenish R. Patel María T. Sánchez-Aparicio Pablo De Ioannes Angeliki Buku Gloria González Aseguinolaza Adolfo García-Sastre Aneel K. Aggarwal 《Structure (London, England : 1993)》2012,20(12):2048-2061
Highlights? The structure of a CARD of human RIG-I ? The mechanism and structural role of phosphorylation are revealed ? In trans interaction with the Helicase and regulatory domains is shown ? Lys172 lies in proximity to the CARD2:helicase-CTD interface 相似文献
6.
Kushol Gupta Joseph E. Curtis Susan Krueger Young Hwang Peter Cherepanov Frederic D. Bushman Gregory D. Van Duyne 《Structure (London, England : 1993)》2012,20(11):1918-1928
Highlights? Missing domains in PFV intasome revealed by SAXS/SANS ? PFV IN undergoes dramatic changes in conformation and oligomerization upon binding DNA ? Strand transfer inhibitors do not alter quaternary structure of PFV intasome 相似文献
7.
Vérane Sommermeyer Claire Béneut Emmanuel Chaplais Maria Elisabetta Serrentino Valérie Borde 《Molecular cell》2013,49(1):43-54
Highlights? Spp1 and histone H3K4 residue are important for meiotic DSB formation ? Spp1 physically interacts with the DSB protein Mer2 ? Spp1 in meiosis is not with RNA Pol II, but on chromosome axes in DSB-rich domains ? Spp1’s PHD finger tethers H3K4me3 regions to chromosome axes for DSB formation 相似文献
8.
Pick-Wei Lau Clinton S. Potter Bridget Carragher Ian J. MacRae 《Structure (London, England : 1993)》2012,20(12):1995-2002
Highlights? DOLORS is a strategy for EM labeling using AviTag and streptavidin ? Internal domains within a large protein can be labeled and identified ? Higher accuracy and precision are achieved by sampling multiple RCT reconstructions ? Offers a powerful method for deciphering moderate-resolution EM structures 相似文献
9.
Highlights? PARP1 recognizes DNA strand breaks through substrate-assisted dimerization. ? PARP1 ZFIII and WGR domains link DNA break recognition to enzyme activation. ? Tankyrase-dependent PARylation regulates Wnt signalling and Cherubism syndrome. 相似文献
10.
.Highlights? Microbial genomes contain multiple clusters for biosynthesis of chemically diverse compounds. ? Most of such genes remain silent, preventing the respective compounds from being discovered. ? Silent gene clusters can be activated and/or heterologously expressed. ? New microbial hosts and tools are needed to make the abovementioned process efficient. ? Synthetic biology will play a major role in the genome-based drug discovery. 相似文献
11.
Errington WJ Khan MQ Bueler SA Rubinstein JL Chakrabartty A Privé GG 《Structure (London, England : 1993)》2012,20(7):1141-1153
Highlights? The crystal structure of the heterotetrameric CRL3-SPOP ubiquitin ligase is reported ? Substrate adaptor proteins are recruited to CRL complexes through a signature motif ? Tandem BTB and BACK domains potentiate assembly and activity of the CRL3-SPOP ligase ? The SPOPL negative regulator creates a molecular rheostat to fine-tune CRL3 activity 相似文献
12.
Ludmil B. Alexandrov Serena Nik-Zainal David C. Wedge Peter J. Campbell Michael R. Stratton 《Cell reports》2013,3(1):246-259
Highlights? Theoretical model describing mutational processes operative in cancer genomes ? Computational framework for deciphering signatures of mutational processes ? Extensive evaluation of the computational framework with simulated data ? Application to mutational catalogs of breast cancer genomes and exomes 相似文献
13.
Erik Verschueren Peter Vanhee Frederic Rousseau Joost Schymkowitz Luis Serrano 《Structure (London, England : 1993)》2013,21(5):789-797
Highlights? Polypeptide fragment interaction patterns predict bound protein-peptide complexes ? Peptide structures can be refined or extended by polypeptide fragment alphabets ? Interacting polypeptide fragment pairs guide domain-domain interface identification ? Interacting polypeptide fragment scaffolds have potential to dock protein domains 相似文献
14.
Michele Tinti Lars Kiemer Stefano Costa Martin L. Miller Francesca Sacco Jesper V. Olsen Martina Carducci Serena Paoluzi Francesca Langone Christopher T. Workman Nikolaj Blom Kazuya Machida Christopher M. Thompson Mike Schutkowski Søren Brunak Matthias Mann Bruce J. Mayer Luisa Castagnoli Gianni Cesareni 《Cell reports》2013,3(4):1293-1305
Highlights? The recognition specificity of 70 SH2 domains is probed ? Recognition specificity diverges faster than sequence ? PepspotDB is a database of protein interactions mediated by SH2 domains 相似文献
15.
Francesco M. Piccolo Hakan Bagci Karen E. Brown David Landeira Jorge Soza-Ried Amelie Feytout Dylan Mooijman Petra Hajkova Harry G. Leitch Takashi Tada Skirmantas Kriaucionis Meelad M. Dawlaty Rudolf Jaenisch Matthias Merkenschlager Amanda G. Fisher 《Molecular cell》2013,49(6):1023-1033
Highlights? EGCs can erase DNA methylation at ICRs in somatic cells after fusion ? EGCs selectively induce 5hmC accumulation at ICRs in the somatic genome ? Conversion of 5mC to 5hmC at these imprinted domains requires Tet1 ? Tet2 depletion results in delayed reprogramming by EGCs 相似文献
16.
Structural Basis for the Versatile Interactions of Smad7 with Regulator WW Domains in TGF-β Pathways
Eric Aragón Nina Goerner Qiaoran Xi Tiago Gomes Sheng Gao Joan Massagué Maria J. Macias 《Structure (London, England : 1993)》2012,20(10):1726-1736
Highlights? HECT ubiquitin ligases and YAP use single or multiple WW domains to select Smad protein targets ? The targets can require multiple binding sites (R-Smads) or unique sites (ISmads) ? Binding sites are phosphorylation dependent (R-Smads) or independent (ISmad) ? Smurf1 WW1-homodimers can stabilize the close and inactive conformation of the ligase 相似文献
17.
Emily Brookes Inês de Santiago Daniel Hebenstreit Kelly J. Morris Tom Carroll Sheila Q. Xie Julie K. Stock Martin Heidemann Dirk Eick Naohito Nozaki Hiroshi Kimura Jiannis Ragoussis Sarah A. Teichmann Ana Pombo 《Cell Stem Cell》2012,10(2):157-170
Highlights? A unique RNAPII variant (S5p+S7p?S2p?) binds PRC targets genome-wide in ESCs ? RNAPII-S5p and PRC coincide in time and localization, and show proportional abundance ? Novel, active PRC-target genes identified in ESCs include metabolic genes ? Active PRC targets switch between on/off (active/PRC) states in the ESC population 相似文献
18.
Lauren K. Slevin Jonathan Nye Derek C. Pinkerton Daniel W. Buster Gregory C. Rogers Kevin C. Slep 《Structure (London, England : 1993)》2012,20(11):1905-1917
Highlights? The Plk4 cryptic polo box is a structurally unique, tandem polo box array: PB1-PB2 ? Full length Plk4 comprises three polo box domains, a unique polo kinase architecture ? The Plk4 PB1-PB2 cassette is required for Asterless binding and centriole targeting ? The Plk4 PB1-PB2 homodimer potentiates Plk4 degradation 相似文献
19.
Zhang X Vadas O Perisic O Anderson KE Clark J Hawkins PT Stephens LR Williams RL 《Molecular cell》2011,41(5):567-578
Highlights? Both nSH2 and cSH2 domains of p85 inhibit basal activity of p110β ? p110β/p85β structure shows cSH2 contacts the C terminus of p110β ? Relief of cSH2 inhibition, unlike nSH2, requires extending beyond the pYXXM motif ? p110β C terminus is critical for phosphorylation of lipids and activation by RTKs 相似文献
20.
Linn Gazda Wojciech Pokrzywa Doris Hellerschmied Thomas Löwe Ignasi Forné Felix Mueller-Planitz Thorsten Hoppe Tim Clausen 《Cell》2013,152(1-2):183-195
Highlights? UNC-45 self-assembles a docking platform for multiple chaperone and client proteins ? Hsp70/90 and myosin bind to specific sites on the TPR and UCS domains of UNC-45 ? The UNC-45 multimer offers the proper spacing to couple myosin folding and assembly ? In vivo, UNC-45 chains support the formation of fully functional sarcomeric repeats 相似文献