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1.
A study has been made of the changes in organization of theprimary wall of xylem fibres and tracheids during the phaseof surface growth using both direct electronmicroscopic observationsand replica techniques. It has been demonstrated that the transversemicrofibril orientation on the inner surface of the cell walldiffers from that on the outer surface. It has been concludedthat the observations made are consistent with some form ofmulti-net mechanism of growth in these cells. Autoradiographicstudies were consistent with this conclusion. Measurements of the variation in path difference in fibres andtracheids undergoing secondary wall formation showed a maximumnear the centre of the cells decreasing towards the cell tips.From these observations and parallel autoradaographic studiesit has been concluded that secondary thickening begins nearthe centre of the cells and progresses towards the cell tips.Since all layers of the secondary wall are lamellated theseobservations imply that more lamellae are present near the centreof the cells than at their tips, so that as secondary thickening proceeds the concentric lamellae of microfibrils grow progressivelytowards the ends of the cells. The lamellae of the secondarywall appear to develop in close association with the limitingcytoplasmic membrane (the plasmalemma). No evidence was obtainedof the inclusion of cytoplasmic components in the developingsecondary wall. At the conclusion of secondary thickening thecytoplasmic membranes retract on to the inner surface of thesecondary wall, forming the so-called terminal lamellawhich shows some fibrillar texture. It has been concluded thatthis appearance reflects some intrinsic texture of the lamellaitself, rather than the presence of cellulose microfibrils. 相似文献
2.
《Annals of botany》1996,77(3):243-250
Morphogenesis of the specialized thread-forming (TF) cells in theStrelitzia reginaeanther was investigated; particular attention was given to the cell walls and the degree of vacuolation. The mass of both cell wall and cytoplasm increased until just before dehiscence. However, cell growth and degradation were largely synchronous processes in the TF cells: before any wall thickening could be observed, degradation of primary cell wall material was already initiated. This degradation continued, with the result that the mature thread cells were eventually fully separated from their surrounding cells.Four stages of development, mainly relating to the degree of cell separation, were established. At stage 1, TF cells began to separate from the subepidermis, while at stage 2 some initial cell wall thickening was taking place. The walls of the TF cell were, at stage 3, thickened considerably (about 1 μm), especially along the radial axes. The texture of these walls was loose due to the presence of large intermicrofibrillar regions, and the previously vacuolated cells were filled with cytoplasm. Longitudinal sections revealed conical gaps in the thick cell wall over the plasmodesmata. Just before dehiscence (late stage 3), the TF cells separated from each other and the subepidermis to such an extent that only plasmodesmata and fibrillar wall remnants kept the files of TF cells in place. The released uniseriate threads were classified as stage 4. (Occasionally the threads were multicellular but only where the transverse walls had not separated from each other.) The threads had thinner cell walls than the TF cells at stage 3 and were vacuolated. 相似文献
3.
Sawidis T 《Protoplasma》2008,233(1-2):95-106
Cadmium had a highly toxic effect on pollen germination and tube growth, which were greatly inhibited as metal concentrations increased. Cadmium concentrations up to 10(-2) M completely stopped pollen germination and pollen showed an increasing tendency to burst within 1 h. At low concentrations, the metal caused a slight stimulation of pollen germination, growth rate and tube elongation at the initial stages of tube development. Comparing the two plants studied, cadmium was more toxic for Nicotiana tabacum than for Lilium longiflorum pollen. Pollen tubes showed a range of strong morphological abnormalities, characterized by uneven or aberrant growth, including apical branching or swelling at the tip of the pollen tube. Cell wall intrusions at or near the tip were evident on the inner side, whereas a loose network formed from fibrillar material was observed on the outer layers. After prolonged cadmium exposure, round (ball-like) aggregates were embedded in a fine fibrillar network. Increased cadmium concentrations (10(-3)-10(-2) M) decreased or completely paralyzed cytoplasmic streaming. No typical cytoplasmic zonation existed, while cell organelles (plastids, lipid droplets) were relocated toward the tip. The vesicular apical zone was drastically reduced, with vesicles dispersed into the subapical region. Mitochondria were distributed throughout the subapical region and among the vesicles of the tube apex. Visible ultrastructural changes in cell organelles were not observed. 相似文献
4.
Paul B. Green 《The Journal of cell biology》1960,7(2):289-296
5.
M. Gabriel 《Folia microbiologica》1984,29(5):365-374
During cultivation in a nutrient medium with snail gastric juice the protoplasts ofRhizopus nigricans produce an incomplete cell wall and grow. A true growth, associated with nuclear division, is involved. Morphology of growth
of the formations is determined by the structure of the incomplete cell wall. When the incomplete wall is formed by a thin
fibrillar net the growing formation assumes the physically optimal shape—i.e. a sphere; when the net is dense polar growth predominates. The degree of construction of the new wall depends on the activity
of snail gastric juice enzymes which decreases during the cultivation. When fresh snail enzymes were added at certain intervals,
only a fine fibrillar net was formed on the surface of growing protoplasts. The formations grew for up to 8 d under these
conditions, reached several hundred μm in size and the number of nuclei increased up to 80-fold. When the blocking of the
wall synthesis was interrupted, a complete cell wall regenerated on the surface of these giant formations and a reversion
to hyphae was observed. The incomplete cell wall functions as a passive morphogenetic factor: It can influence the morphology
of the growing protoplasts but it cannot induce reversion to hyphase and secure the permanent existence of these structures. 相似文献
6.
A transmission and scanning electron microscope study was madeof cell wall development in the root velamen of Ansellia gigantea.The pattern of fibrillar deposition in the primary walls wasestablished by means of shadowed surface preparations. The helicalwall thickenings were shown to originate by the localised appositionof bundles of orientated 2030 nm cellulose fibrils. Thisproceeds from near the tips of the radially elongated cells.Although microtubules were associated with later stages of helixdeposition, there was no evidence for cytoplasmic pre-patteming.The large wall perforations, circumscribed by secondary walldepositions, develop at the sites of pit fields. The implicationsof the present observations are discussed, particularly as theyappear to relate to general principle of wall development intracheary elements. 相似文献
7.
Electron-energy-loss spectroscopic imaging of calcium and nitrogen in the cell walls of apple fruits 总被引:5,自引:0,他引:5
I. Max Huxham Michael C. Jarvis Lynette Shakespeare Colin J. Dover David Johnson J. Paul Knox Graham B. Seymour 《Planta》1999,208(3):438-443
Changes in texture are an integral part of ripening in most fleshy fruits and these changes are thought to be determined,
primarily, by alterations in cell wall structure. Electron energy loss spectroscopy (EELS) imaging was used to obtain quantitative
information on the levels of calcium and nitrogen in the cell walls of apple (Malus domestica Borkh. cv. Cox's Orange Pippin) fruits. Samples of fruit cortex were prepared for EELS by high-pressure freezing and molecular
distillation drying to minimize loss and redistribution of soluble cell wall components such as calcium. The EELS imaging
successfully resolved calcium and nitrogen levels in the middle lamella and primary cell wall. When the elemental compositions
of the cell walls of Cox's apples from two sites in the UK were compared at harvest or after 6 months storage, the orchard
which always produced consistently firmer fruit had significantly lower levels of cell wall calcium and higher levels of cell
wall nitrogen. This result was unexpected since firm texture in apples and other fruits has been commonly associated with
elevated levels of fruit calcium. The nitrogen-rich material in the sections used for EELS was insoluble in acidified methanol,
indicating that it represented a high-molecular-weight component in the cell wall. Furthermore, total tissue hydroxyproline
levels were greatest in material with elevated cell wall nitrogen, suggesting enhanced levels of wall structural proteins
in the tissue. These data indicate a correlation between increased amounts of cell wall nitrogen and firm fruit texture. The
possible role of cell wall proteins in determining the textural properties of fruit tissue is discussed.
Received: 19 November 1998 / Accepted: 28 January 1999 相似文献
8.
DEMONSTRATION OF A FIBRILLAR COMPONENT IN THE CELL WALL OF THE YEAST SACCHAROMYCES CEREVISIAE AND ITS CHEMICAL NATURE 总被引:7,自引:1,他引:6
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The ultrastructure of isolated cell walls of Saccharomyces cerevisiae from the log and stationary phases of growth was studied after treatment with the following enzymes: purified endo-β-(1 → 3)-glucanase and endo-β-(1 → 6)-glucanase produced by Bacillus circulans; purified exo-β-glucanase and endo-β-(1 → 3)-glucanase produced by Schizosaccharomyces versatilis; commercial Pronase. While exo-β-glucanase from S. versatilis had no electron microscopically detectable effect on the walls, Pronase removed part of the external amorphous wall material disclosing an amorphous wall layer in which fibrils were indistinctly visible. Amorphous wall material was completely removed by the effect of either endo-β-(1 → 3)- or endo-β-(1 → 6)-glucanase of B. circulans or by a mixture of the two enzymes. As a result of these treatments a continuous fibrillar component appeared, composed of densely interwoven microfibrils resisting further action by both of the B. circulans enzymes. The fibrillar wall component was also demonstrated in untreated cell walls by electron microscopy after negative staining. Because of the complete disappearance of the fibrils following treatment with the S. versatilis endo-β-(1 → 3)-glucanase it can be concluded that this fibrillar component is composed of β-(1 → 3)-linked glucan. Bud scars were the only wall structures resistant to the effect of the latter enzyme. 相似文献
9.
The disc cell wall facing the secretory cavity in lipophilic glands of Cannabis was studied for origin and distribution of hyaline areas, secretory vesicles, fibrillar matrix and particulate material. Secretions evident as light areas in the disc cell cytoplasm pass through modified regions in the plasma membrane and appear as hyaline areas in the cell wall. Hyaline areas, surrounded with a filamentous outline, accumulate near the wall surface facing the secretory cavity where they fuse to form enlarged hyaline areas. Fibrillar matrix is related to and may originate from the dense outer layer of the plasma membrane. This matrix becomes distributed throughout the wall material and contributes in part to the composition of the surface feature of secretory vesicles. Thickening of the cell wall is associated with secretions from the disc cells that facilitates movement of hyaline areas, fibrillar matrix and other possible secretions through the wall to form secretory vesicles and intervesicular materials in the secretory cavity. The outer wall of disc cells in aggregate forms the basilar wall surface of the secretory cavity which facilitates the organization of secretory vesicles that fill the secretory cavity. 相似文献
10.
H. J. Pluymaekers 《Protoplasma》1982,112(1-2):107-116
Summary The cell wall of root hairs ofLimnobium stoloniferum is composed of two fibrillar layers: an outer layer with a dispersed texture and an inner layer with a helicoidal texture. In stained oblique sections the helicoidal layer appears as a series of bow-shaped structures. In sections which were shadow-casted after the embedding medium was removed, the following properties of the helicoidal layer can be directly observed. (1) It is build up of superimposed lamellae. (2) Each lamella consists of parallel oriented microfibrils. (3) Going into the helicoidal layer, there is a counter-clockwise discontinuous rotation of the microfibril orientation in successive lamellae. (4) Between adjacent lamellae the average angular displacement of the microfibril orientation is about 23 degrees. The dispersed outer layer is also polylamellated, but with randomly arranged microfibrils in each lamella. Both layers are present in the lateral wall as well as in the apical wall of the root hairs. Observations indicate that in the cell wall of the tip the parallel oriented microfibrils of the outermost helicoidal lamellae become distorted towards a dispersed arrangement. The suggestion is made that the dispersed outer layer is derived from the helicoidal layer. 相似文献
11.
Vegetative myxamoebae of Acytostelium leptosomum, a cellular slime mold, have the appearance of typical eucaryotic cells. The presence of dictyosomes has been established. Elongation of the cells during aggregation and culmination appears to be mediated by dense bundles of microfibrils traversing the cells longitudinally. Microtubules are present; however, they are randomly oriented and no correlation can be made with cell elongation or with the direction of the cellulose microfibrils within the stalk. A variety of vesicles, multivesicular bodies, and lysosome-like vacuoles seems to be involved in producing and transporting stalk material to the vicinity of the stalk. However, only rarely do the vesicles empty their contents directly to the outside of the cells. It seems rather that the fibrillar material of the stalk is assembled near or directly at the plasmalemma, and can then be seen to stream away and become an integral part of the stalk. An unusual structure, the H-body, is formed in great abundance during culmination indicating its possible involvement in stalk synthesis. The H-bodies are removed from the cells prior to spore formation together with other portions of the cytoplasm at least partly by a process involving autophagic vacuoles. These vacuoles, which are also present in the spores, appear to be part of a rather complex and extensive vacuolar apparatus including the food vacuoles, contractile vacuoles, lysosome-like structures, and possibly the H-bodies. The spore coat consists of a heavy outer wall with a fibrillar substructure and two thin, dense bands lining the inside of the plasmalemma. The fibrillar nature of both the outer spore wall and the stalk was accentuated by using barium permanganate to stain sectioned material. 相似文献
12.
A I Radchenko 《Tsitologiia》1992,34(2):91-96
An electron cytochemical study of glycoproteins and glycolipids was made for the mature sarcocysts of Sarcocystis muris. Glycoprotein structures as branched fibrilles were seen on the surface of the sarcocyst wall. The fibrillar and granular glycoprotein structures were found in the ground substance of sarcocysts near the cyst wall and in the septae. In the plasmalemma of two types of cyst stages (merozoites and intermediate cells), glycoprotein fibrillar structures were revealed connecting these two cell types with each other. The third type cyst stages, i.e. the metrocytes, are situated separately without any fibrillar connections between them and other cyst stages being observed. This question is discussed in terms of the problem of cytodifferentiation. The fibrillar and granular glycoprotein material is scattered over the cytoplasm of the cyst stages, being especially concentrated in micronemes, rhoptries and around amylopectin granules. The control ultrathin sections were treated with saliva or pronase for the aims of protein identification in the material under study. In addition to glycoprotein, some glycolipids material was detected in the sarcocysts in the form of drops surrounded with thin glycoproteinaceous layers. Glycolipids were found in the ground substance of sarcocysts near the cyst stages and in the parasite cell cytoplasm around the micronemes and rhoptries. The data obtained are discussed in connection with the functional role glycoproteins and glycolipids play in S. muris. 相似文献
13.
Cell surface arabinogalactan-proteins and their relation to cell proliferation and viability 总被引:4,自引:0,他引:4
Summary We have used high-pressure freezing followed by freeze substitution (HPF/FS) to preserve in vivo grown lily pollen tubes isolated from the style. The results indicated that HPF/FS (i) allows excellent preservation of the pollen tubes, (ii) maintains in situ the stylar matrix secreted by the transmitting tract cells, and (iii) preserves the interactions that exist between pollen tubes. Particular attention has been given to the structure of the pollen tube cell wall and the zone of adhesion. The cell wall is composed of an outer fibrillar layer and an inner layer of material similar in texture and nature to the stylar matrix and that is not callose. The stylar matrix labels strongly for arabinogalactan proteins (AGPs) recognized by monoclonal antibody JIM13. The zone of adhesion between pollen tubes contains distinct matrix components that are not recognized by JIM13, and apparent cross-links between the two cell walls. This study indicates that HPF/FS can be used successfully to preserve in vivo grown pollen tubes for ultrastructural investigations as well as characterization of the interactions between pollen tubes and the stylar matrix.Abbreviations AGPs
arabinogalactan proteins
- FS
freeze substitution
- HPF
high-pressure freezing 相似文献
14.
Plasma membrane particles demonstrable by the freeze-etching technique, play, according to some authors, a role in the cell
wall synthesis. On a model of yeast protoplast capable of regenerating the cell wall we studied the morphology of plasma membrane
particles and the synthesis of the fibrillar cell wall component following a treatment with various enzymes and with lysolecithin.
The enzymes used included proteases (trypsin, papain, pronase), polysaccharide-splitting enzymes (snail enzyme complex, mannosidase),
phospholipases (A, C, D) and lipase. Upon treating living protoplasts with these substances in no case did we observe any
morphologically demonstrable change in the particle structure or in their distribution in the plasma membrane. The fibrillar
cell wall component was synthetized even in the presence of proteases and phospholipases. If the plasma membrane particles
are assumed to represent enzyme systems synthesizing the cell wall component then in living protoplasts they are not located
on the outer plasma membrane face or else are protected by some mechanism against the action of the corresponding enzymes. 相似文献
15.
Summary A cytological study was carried out to describe the initial steps of infection of maize roots by the soil fungusSporisorium reilianum f. sp.zeae. Morphogenetic changes of the fungal cells were induced in the presence of maize roots. Extensive hyphal growth led to the formation of a thick fungal layer colonising the maize root surface. This structure is original in interactions of members of the family Ustilaginaceae with plants. In the thick fungal layer, we observed fimbriae inserted into the host cell wall, suggesting a direct role of these fibrillar structures in cell adhesion and infection processes. During infection, no reaction of host cells was observed. In this way, the fungus acts as a biotrophic endophyte during the initial steps of infection. 相似文献
16.
The development and structure of the guard cell walls of Funaria hygrometrica Hedw. (Musci) were studied with the light and electron microscopes. The stoma consists of only one, binucleate guard cell as the pore wall does not extend to the ends of the cell. The guard cell wall is thinnest in the dorsal wall near the outer wall but during movement is most likely to flex at thin areas of the outer and ventral walls. The mature wall contains a mottled layer sandwiched between two, more fibrillar layers. The internal wall layer has sublayers with fibrils in axial and radial orientations with respect to the pore. During substomatal cavity formation, the middle lamella is stretched into an electron dense network and into strands and sheets. After stomatal pore formation, the subsidiary cell walls close to the guard cell become strikingly thickened. The functional implications of these results are discussed. 相似文献
17.
Summary Light and transmission electron microscopy were used to examine hyphal tip cells of the fungusAllomyces macrogynus (Chytridiomycetes). A well defined apical body, i.e., Spitzenkörper, was observed at the extreme apex of hyphal cells. This distinctive, spherical cytoplasmic region consisted of a granular matrix devoid of ribosomes and most organelles. To our knowledge this is the first report describing such a structure in hyphae of an aseptate fungus. Vesicles (45–65 nm diameter) were concentrated in the peripheral cytoplasm of the apex, while relatively few were observed within the Spitzenkörper. Filasomes, spherical patches of dense fibrillar material containing a microvesicle core, were abundant in the apical regions near the plasma membrane. Microtubules traversed the Spitzenkörper at various angles and were in close association with the plasma membrane. Microfilaments were observed as individual elements in the cytoplasm or were organized into bundles. Individual microfilaments were frequently in close association with the plasma membrane, vesicles and microtubules. In the immediate subapical region mitochondria, multivesicular bodies, microbodies, Golgi equivalents and nuclei were abundant.Abbreviations CW
cell wall
- F
filasome
- M
mitochondria
- N
nucleus
- PM
plasma membrane
- TEM
transmission electron microscopy 相似文献
18.
Biochemical study of the relationship of extracellular glucan to adherence and cariogenicity in Streptococcus mutans and an extracellular polysaccharide mutant. 总被引:10,自引:0,他引:10
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A mutant of Streptococcus mutans, GS-5, which differed in extracellular polysaccharide (EPS) produced from sucrose, was used to study the role of EPS in the production of dental caries. The mutant proved to be identical to the parent strain in sugar fermentation, growth rate, and serotype. Strain GS-5 synthesized an EPS, which in electron micrographs appeared to be of fibrillar structure, whereas the mutant produced no fibrillar material but only a globular EPS. Analysis of the EPS revealed that about 30% of the glucose units in the GS-5 polymer carried (1-3)-like bonds either as branch points or as part of the linear backbone and that the mutant material contained only about 3% of these linkages. When grown in sucrose broth, the proportion of the mutant culture adherent to the glass vessel was dramatically less than that of the parent strain. Caries scores produced in conventional rats by the mutant were significantly lower than those obtained with the parent strain. Since the only difference discovered between strain GS-5 and the mutant was the inability of the mutant to synthesize either a fibrillar EPS or an EPS with more than about 3% (1-3)-like linkages, it was concluded that the fibrillar EPS of strain GS-5 contained about 30% (1-3)-like linkages and was necessary for adherence of the bacteria to surfaces and for production of dental caries in test animals. 相似文献
19.
Inhibitory effect of 2-deoxy-d-glucose on the formation of the cell wall in yeast protoplasts 总被引:4,自引:1,他引:3
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2-Deoxy-d-glucose (2DG) acted as a competitive inhibitor of the synthesis of cell wall components in Saccharomyces cerevisiae protoplasts. The synthesis of fibrillar glucan cell wall component was inhibited at a glucose to 2DG ratio of 4:1 in the cultivating medium. The completion of the formation of cell wall by the synthesis of the amorphous mannan-protein cell wall component was inhibited at a glucose to 2DG ratio of about 20:1. The inhibition could be reversed by increasing the glucose to 2DG ratio in the nutrient medium. No incorporation of 2DG into fibrillar glucan cell wall component was observed. 相似文献
20.
Cells of Pseudomonas phaseolicola were observed entrapped against plant cell walls in both susceptible (Red Kidney) and resistant (Red Mexican) cultivars of French bean (Phaseolus vulgaris). After staining of samples with ruthenium red for electron microscopy pectic polysaccharide within plant cell walls became particularly well contrasted as did fibrillar material connecting bacteria to the plant cell walls. In places this fibrillar material appeared to emanate from the pectic polysaccharide in the plant cell wall, and the plant cell wall surface was eroded at such points. Ruthenium red also stains acidic, bacterial extracellular polysaccharide (EPS) and some of the fibrillar material in intercellular spaces is probably from this source. It is possible that bacteria become attached through an interaction between EPS and Pectic polysaccharide in plant cell walls. 相似文献