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1.
1. Arginase was found to be present in the intestine in all species of Annelida, Arthropoda and Chordata studied. 2. The activity of intestinal arginase differs from species to species, the differences reaching two orders of magnitude (100 x). 3. The highest activity of intestinal arginase was observed in the rodents (mouse, rat, hamster). 4. In animals in which the enzyme activity was high or moderately high, arginase activity showed topographical differentiation along the long axis of the intestine.  相似文献   

2.
1. The activity of rhodanese in different tissues of some domestic animals was measured. 2. Rhodanese was present in all tissues studied. 3. The activity of rhodanese in most tissues of sheep was higher than other animals studied. 4. In sheep and cattle the epithelium of rumen, omasum and reticulum were the richest sources of rhodanese. Significant activity of rhodanese was also present in liver and kidney. 5. In camel the liver contained the highest level of rhodanese followed by lung and rumen epithelium. Camel liver contained a third of the activity of sheep liver. 6. Equine liver had a third of the activity of sheep liver. Other tissues showed low levels of rhodanese activity. 7. Dog liver contained only 4% of the activity of sheep liver. In this animal, brain was the richest source of rhodanese. 8. The results are discussed in terms of efficacy of different tissues of animals in cyanide detoxification.  相似文献   

3.
P K Reddi  W E Knox  A Herzfeld 《Enzyme》1975,20(5):305-314
Significant amounts of arginase activity were found in homogenates of submaxillary salivary gland and epididymis, as well as of liver, kidney, mammary gland, and small intestine. The isoelectric point of arginase solubilized from kidney was at pH 7.0 in contrast to that of pH 9.4 characteristic of hepatic arginase in rat. The isozymic variants of arginase in the different tissues were identified by their electrophoretic migration on polyacrylamide gels and by titration of the enzymes against antibody prepared against purified rat liver arginase. Antibody titrations confirmed the indications obtained by electrophoresis that one type of arginase is limited to hepatic tissues (and possibly submaxillary gland) while the other type is found in all other tissues. The physiological role of arginase in hepatic tissues has been previously associated with the urea cycle; the possible function of arginase in proline synthesis in other tissues remains to substantiated.  相似文献   

4.
The activities and regulatory patterns of arginase and ornithine aminotransferase (OAT) of the liver (a mitotic tissue) and kidney cortex (a post-mitotic tissue) of immature, adult, and senescent male rats were studied. The activities of the liver enzymes were highest in the immature rat and decreased gradually with age. However, in the kidney cortex, the activity of arginase was highest and decreased significantly thereafter while that of OAT shows no significant change throughout the life span of the rat. Further, the activity of kidney cortex arginase was approximately 1/20th of that of the liver enzyme. Adrenalectomy and hydrocortisone treatments altered the activity of arginase in both tissues and that of OAT in the liver only. However, the kidney cortex OAT was not responsive towards these treatments. Actinomycin D inhibited the hydrocortisone-mediated induction of arginase of both the liver and kidney cortex and that of the liver OAT.  相似文献   

5.
Hepatocellular carcinoma (HCC) is one of the most common tumors worldwide affecting preferentially patients with liver cirrhosis. The studies were performed on tissues obtained during surgery from 50 patients with HCC, 40 with liver cirrhosis and 40 control livers. It was found that arginase activity in HCC was nearly 5- and 15-fold lower than in cirrhotic and normal livers, respectively. Isoenzymes AI (so-called liver-type arginase) and AII (extrahepatic arginase) were identified by Western blotting in all studied tissues, however the amount of AI, as well as the expression of AI-mRNA were lower in HCC, in comparison with normal liver, and those of AII were significantly higher. Since HCC is arginine-dependent, and arginine is essential for cells growth, the decrease of AI may preserve this amino acid within tumor cells. Concurrently, the rise of AII can increase the level of polyamines, compounds crucial for cells proliferation. Thus, both arginase isoenzymes seem to participate in liver cancerogenesis.  相似文献   

6.
Arginase activity is elevated in livers of diabetic animals compared to controls and there is evidence that this is due in part to increased specific activity (activity/mg arginase protein). To investigate the molecular basis of this increased activity, the physicochemical and kinetic properties of hepatic arginase from diabetic and control mice were compared. Two types of arginase subunits with molecular weights of 35,000 and 38,000 were found in both the diabetic and control animals and the subunits in these animals had similar, multiple ionic forms. Kinetic parameters of purified preparations of arginase for arginine (apparent Km and Vmax values) and the thermal stability of these preparations from diabetics and controls were also similar. Furthermore, no difference was found in the distribution of arginase activity among different subcellular liver fractions. Separation of basic and acidic oligomeric forms of arginase by fast-protein liquid chromatography resulted in a slightly different distribution of activity among the forms in the normal and diabetic group. The apparent Km values for Mn2+ of the basic form of the enzyme were 25 and 33 microM for the enzyme from normal and diabetic animals, respectively; for acidic forms, for which two apparent Km values were measured, the values were 8 and 197 microM for arginase from controls and 35 and 537 microM from diabetics. These results indicate that in diabetes, while no marked changes in the physicochemical characteristics of arginase are obvious, some changes are found in the interaction of arginase with its cofactor Mn.  相似文献   

7.
In order to establish if the urea found in foetal fluids in sheep could be of foetal origin and whether there are changes in the ability of ovine liver to synthesise urea during foetal and postnatal development, the rates of urea production from ammonium and bicarbonate ions have been measured in liver and kidney slices from animals aged from 50 days conceptual age to 16 weeks after birth, and in pregnant and non-pregnant ewes. The activities of five enzymes directly involved in the biosynthesis of urea have also been determined.Urea was found to be synthesised by foetal liver from at least 50 days conceptual age at rates similar to those observed in adult ewes. Highest rates of urea synthesis per unit weight of liver were found immediately after birth. In the liver there were significant positive correlations between the rates of urea synthesis by slices and the activities of carbomoyl phosphate synthase (ammonia) (EC 2.7.2.5), argininosuccinate synthetase (EC 6.3.4.5) and argininosuccinate lyase EC 4.3.2.1). Ornithine carbomoyl transferase (EC 2.1.3.3) activity was highest in the livers of ruminating animals. Hepatic arginase activity (EC 3.5.3.1) was highest during the late foetal life and in the mature foetuses the activity was ten-fold greated than that in maternal liver.Urea was not synthesised from ammonia and bicarbonate in kidney slices and neither ornithine carbomoyl transferase activity nor argininosuccinate synthetase activity could be detected. The activity of renal arginase was at least 70 times less than that found in the liver and the highest activity was found in ruminating lambs.The changes observed in the activities of the urea cycle enzymes during development have been contrasted with those reported to occur in other species. It is concluded that there is no single factor regulating the activities of the five enzymes directly concerned with urea synthesis during development. The results support the hypothesis that in mammals the ability of the liver to synthesise urea in foetal life is related to renal development.  相似文献   

8.
Distribution of acylase in different tissues of nine species of animals was studied. The following types of nitrogen elimination were distinguished: ammoniatelic (fish), uricotelic (birds) and uriotelic (amphibians, mammalians). The enzymic activity was estimated in the tissues of the brain, lung, muscle, liver, kidney, spleen, pancreas, small intestine and blood serum. The acylase activity was found in the kidney, liver and pancreas. Its level in the kidney increases with the animal weight growth, the enzyme activity being observed only in the cortical layer.  相似文献   

9.
The electrophoretic behaviour of arginase in the tissue extracts of rat, beef, lizard and frog was studied by bidirectional polyacrylamide gel electrophoresis. The enzyme from rat liver and submaxillary gland migrated to the cathode with the activity concentrated in a single peak. Arginase from beef liver emerged as a single peak of anodal migration with a significant shoulder in the sample gel. Frog liver and kidney enzymes also appeared as single peaks with a distinct anodal movement. The activity in mammalian kidney and lizard liver and kidney resolved into two peaks of anodal migration suggesting the presence of two isoenzymes of arginase in these tissues.  相似文献   

10.
Transgenic animals are a promising source of pharmaceutically-relevant proteins or as a source of organs for xenotransplantation. Beside other posttranslational modifications, glycosylation has been shown to be a critical parameter for the correct function of several glycoproteins. To analyse the contribution of alpha 1,6-fucosylation to N-glycan variability, we partly purified alpha 1,6-fucosyltransferase (alpha 1,6-Fuc-T) activities from various tissues (brain, lung, heart, liver) of agriculturally-relevant animals (porcine, sheep, bovine, rabbit, chicken) and compared some of their biochemical properties. All tissues displayed alpha1,6-Fuc-T activity, although at different levels. No differences were observed in their stability against chemicals, temperature or time, whereas the activities were distinguishable by their pH-optima and their cation preferences. Similarities were found for tissues between species. Lung and heart enzymes showed a narrow pH-optimum around pH 6.0 and an enhanced activity in the presence of divalent cations. alpha 1,6-Fuc-T activities in brain and liver were characterised by a broad pH-optimum from 5.5 to 8.0. Some activities of these tissues were decreased by the addition of EDTA, while others did not show any influence of EDTA or divalent cations. From the significant differences of the alpha 1,6-Fuc-T activities in the tissues, it is possible to hypothesise the presence of more than one single alpha 1, 6-Fuc-T in mammalian tissues.  相似文献   

11.
The effects of dietary intake of vitamin E and selenium on arginase activity in the liver, kidneys, and heart of rats treated with high doses of prednisolone were investigated. Rats were divided into five groups. Groups 3, 4, and 5 received a daily supplement in their drinking water of vitamin E, Se, and a combination of vitamin E and Se, respectively, for 30 days. For 3 days subsequently, the control group (group 1) was given a placebo, and the remaining four groups were injected intramuscularly with prednisolone. The tissue samples were collected from each group at 4, 8, 12, 24, and 48 h after the last administration of prednisolone. In the group treated with prednisolone alone, arginase activity in the liver was found to have increased at all the time periods, whereas it had decreased significantly in the heart at 48 h. Arginase activity in the kidneys was not affected by prednisolone. Compared to the control and prednisolone groups, arginase activity in the kidneys and heart of the vitamin E- and Se-supplemented groups was found to be significantly increased at all time periods, however, no difference was seen in the combination group. Arginase activity in the liver of the vitamin E-supplemented group was found to have decreased at all time periods, however, in the Se group compared to the prednisolone group it had reduced at 24 and 48 h only. In the combination group compared to the prednisolone group, liver arginase activity increased constantly up to 12 h returning to normal values at 48 h. Vitamin E and Se in combination may prevent the changes in arginase activity in various tissues caused by prednisolone.  相似文献   

12.
The arginase present in mouse brain and liver was studied in order to determine if the activity in both tissues was due to the same enzyme. Mouse liver contains only one arginase enzyme whereas mouse brain contains two. One of the arginases in the brain is distinct from the liver enzyme as determined by fractionation on DEAE-cellulose, CM-cellulose and disc gel electrophoresis. The second enzyme from brain tissue has the same properties as the liver enzyme when subjected to these same fractionation techniques. However this arginase can be distinguished from the liver enzyme by its Km for arginine heat lability and MnCl2 activation curve. Thus both arginases in brain differ from the liver enzyme. No interconversion of the brain enzymes was detected, and the molecular weight of all the arginases appears to be the same. The observation of multiple distinct brain and liver arginases in mouse brain and liver was confirmed with bovine tissues.  相似文献   

13.
An extensive survey of higher fungi revealed that members of the family Agaricaceae, including Agaricus bisporus, accumulate substantial amounts of urea in their fruit bodies. An important role of the ornithine cycle enzymes in urea accumulation has been proposed. In this work, we present the cloning and sequencing of the arginase gene and its promoter region from A. bisporus. A PCR-probe based on fungal arginase was used to identify the A. bisporus arginase gene from a cDNA library. The arginase cDNA encodes a 311-aa protein which is most likely expressed in the cytosol. Expression of the cDNA in Escherichia coli was established as a His-tagged fusion protein. The arginase gene was used as a molecular marker to study expression and regulation during sporophore formation and postharvest development. The expression of the arginase gene was significantly up-regulated from developmental stage 3 onwards for all the tissues studied. A maximum of expression was reached at stage 6 for both stipe and cap tissue. In postharvest stages 5, 6 and 7 the level of expression observed was similar to normal growth stages 5, 6 and 7. A good correlation was found between arginase expression and urea content of stipe, velum, gills, cap and peel tissue. For all tissues the urea content decreased over the first four stages of development. From stage 4 onwards urea accumulated again except for stipe tissue where no significant changes were observed. The same trend was also observed for postharvest development, but the observed increase of urea in postharvest tissues was much higher.  相似文献   

14.
The adult patterns of arginase isoenzymes in rat intestine, kidney, and brain are nearly identical and consist of two forms, cationic A1 and anionic A4. In this paper, the organ-specific maturation of the enzyme equipment in these tissues is reported. The activity of arginase in all tissues studied could be detected on the 13th to 16th days of gestation. In fetal intestine and kidney the arginase activity is low, and persists up to the weaning time when the rapid, 10-fold rise of the enzyme activity occurs. However, the adult pattern of arginase isoenzymes in these tissues is accomplished in different ways. In the intestine, arginase A1 appears in fetal life and is the only form of the enzyme till the 19th to 21st days of postnatal life when the second form of arginase, A4, appears and rapidly accumulates, being exclusively responsible for the rise of the total enzyme activity at the time of weaning. In kidney, arginase A1 alone is present in the early fetal period. Arginase A4 appears 3-4 days before birth and its activity persists unchanged within the first 2 weeks of postnatal life. The intensive rise in total specific activity of kidney arginase at weaning is due to the accumulation of preexisting arginase A4. In brain, the adult pattern of arginase isoenzymes is achieved earlier than in other tissues. Both forms, A1 and A4, occur on Days 13-14 of gestation.  相似文献   

15.
The content of urea was studied in protein-free filtrates of the liver kidneys, skeletal muscles, myocardium, spleen, brain tissues and blood serum as well as in urine of 1, 3, 6, 12 and 24-month rats. It is shown that at the age of 6 months the content of urine in most tissues under study is significantly decreased (by 42-73%), at the age of 12 months in the spleen and at the age of 24 months in the brain tissues as compared to the one-month animals. The level of urine decrease in the liver and brain tissues of 24-month animals is less pronounced than in other tissues, that corresponds to age peculiarities of their protein metabolism. A decrease of blood consumption per weight unit and a relative increase in the amount of nitrogen excreted with urine are observed with ageing. The arginase activity in the liver decreases essentially only in 3-month animals. A conclusion is drawn that peculiarities of food consumption and the character of changes in the urea content in tissues and urine are adaptation manifestation of an age decrease in the intensity of nitrogen metabolism and protein demand of the organism.  相似文献   

16.
The effects of lysine administration on arginine and ornithine liver levels were studied in normal and urease-treated rats. L-Arginine injections produced a rise in liver arginine with a parallel increase in liver ornithine. Pretreatment with L-lysine resulted in an elevation in liver arginine. Administration of lysine to urease treated rats induced a significant increase in liver arginine content with a parallel drop in ornithine/arginine ratio. A similar decrease in ornithine/arginine ratio due to lysine administration was observed in animals, in which arginine and ornithine levels had been raised by loading with arginine. The mechanism of the lysine effect is most likely by inhibition of liver arginase activity in vivo.  相似文献   

17.
The activity of acid phosphatase in liver macrophage aggregates (MA-AP) of different fish species was used as a marker for a pollution-induced modulation of the digestive capacity of phagocytes, since functions of the non-specific immune response play a central role in the maintenance of animals' health. Based upon the investigation of more than 900 individual flounders (Platichthys flesus) and mullets (Liza aurata), natural variations, gender-specific differences and pollution-induced alterations in AP activity are demonstrated in this study. MA-AP activity was dependent on temperature and season but, nevertheless, distinctions between differently polluted areas were visible in all sampling campaigns with lowest MA-AP activity in fish from the polluted areas of the German Bight and the Israeli coast of the Mediterranean Sea. For organochlorine contaminants, as well as for mercury and copper, a significant correlation could be observed between residue concentrations in fish tissues and MA-AP activity. In all cases, except mercury which showed a positive correlation, AP activity was suppressed in animals with a high contaminant burden. MA-AP activity turned out to give reliable and consistent results for a quantification of immunomodulation in both fish species.  相似文献   

18.
We have previously reported the occurrence of multiple forms of drug-metabolizing enzymes in camel tissues. Here, we investigate glutathione (GSH)-dependent redox homeostasis, reactive oxygen species (ROS) production and mitochondrial respiratory functions in camel tissues and compare them with imported domestic goats and laboratory rats and mice. Cytochrome P450 2E1 (CYP 2E1) and GSH-metabolizing enzymes were differentially expressed in the liver and kidney of these animals. Camel liver has significantly lower GSH pool than that in goats, rats and mice. Mitochondria isolated from the tissues of these animals showed a comparable ability to metabolize specific substrates for respiratory enzyme complexes I, II/III and IV. These complexes were metabolically more active in the kidney than in the liver of all the species. Furthermore, the activity of complex IV in camel tissues was significantly lower than in other species. On the other hand, complex II/III activity in camel kidney was higher compared to the other species. In addition, as expected, we observed that inhibitors of these enzyme complexes augment the production of mitochondrial ROS in camel and goat tissues. These results help to better understand the metabolic ability and adaptation in desert camels in comparison with domestic goats and laboratory rats and mice since they are exposed to different environmental and dietary conditions. Our study may also have implications in the pharmacology and toxicology of drugs and pollutants in these species.  相似文献   

19.
Purification and properties of arginase of rat kidney   总被引:7,自引:1,他引:6       下载免费PDF全文
l-Arginase from rat kidney was partially purified and some properties were compared with those of l-arginase of rat liver. The kidney enzyme was firmly bound to the mitochondrial fraction and after solubilization required arginine or an unknown factor in tissue extracts for stabilization after dialysis. The two enzymes differed also in stability with respect to acetone treatment, heating or freezing. In further contrast with liver arginase, arginase from kidney was not adsorbed to CM-cellulose at pH7.5 and its activity was not increased by incubation with Mn(2+). Other differences were seen in relative specificities for substrates, ratio of hydrolysis rates with high and low concentrations of arginine and effects of certain inhibitors. Antisera prepared to pure liver arginase did not cross-react with partially purified kidney arginase.  相似文献   

20.
The activities of key antioxidant enzymes in two endemic Baikalean amphipod species: Pallasea cancelloides (Gerstf), Eulimnogammarus verrucosus (Gerstf) and the widely distributed Palearctic species Gammarus lacustris (Sars) were studied. This work was done to prove or disprove the hypothesis that Baikalean endemics have specifics in antioxidants system different from Palearctic species. The activities of antioxidant enzymes peroxidase, catalase and glutathione-S-transferase were measured in different sections (tagmata) of the amphipods' bodies as well as in different size groups. Well expressed tagma-related differences in peroxidase activity as well as smaller differences in catalase activity were shown in all studied species. There were no measured differences in glutathione-S-transferase activity among body sections. The existence of size-related changes in some antioxidant enzymes and the difference in such changes between Baikalean and Palearctic amphipods were noted. A significant increase in peroxidase activity with the size was found in both Baikalean species while a significant decrease in peroxidase activity was observed in the Palearctic G. lacustris. In Baikalean P. cancelloides, a significant decrease of catalase activity with the increase in age of crustaceans was noted, while in E. verrucosus no such relationship was found. In the Palearctic G. lacustris, a significant increase in catalase activity with the increase in size was noted. All species are shown to have no size-related differences in glutathione-S-transferase activity. The differences between species as well as between both different tagmata and size-classes within a particular species were estimated. It was assumed that the estimated differences in enzymes activity most likely depend on interspecific variation, rather than on conditional specifics in Lake Baikal.  相似文献   

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