共查询到20条相似文献,搜索用时 31 毫秒
1.
This review is devoted to the analysis of publications on studies of phlorotannins, which are polyphenolic metabolites of brown algae. Their properties, the pathway of biosynthesis, and the structural diversity and classification of phlorotannins, as well as the methods for their isolation and determination of their metabolic profiles, are examined. The antioxidant, antitumor, and antimicrobial activities of phlorotannins and their role in the regulation of enzyme activity are discussed. These properties of phlorotannins determine the prospects of using phlorotannins in pharmacology. 相似文献
2.
Gene expression changes related to the production of phenolic compounds in potato tubers grown under drought stress 总被引:1,自引:0,他引:1
Christelle M. Andr Roland Schafleitner Sylvain Legay Isabelle Lefvre Carlos A. Alvarado Aliaga Giannina Nomberto Lucien Hoffmann Jean-Franois Hausman Yvan Larondelle Danile Evers 《Phytochemistry》2009,70(9):1107-1116
Polyphenols represent a large family of plant secondary metabolites implicated in the prevention of various diseases such as cancers and cardiovascular diseases. The potato is a significant source of polyphenols in the human diet. In this study, we examined the expression of thirteen genes involved in the biosynthesis of polyphenols in potato tubers using real-time RT-PCR. A selection of five field grown native Andean cultivars, presenting contrasting polyphenol profiles, was used. Moreover, we investigated the expression of the genes after a drought exposure. We concluded that the diverse polyphenolic profiles are correlated to variations in gene expression profiles. The drought-induced variations of the gene expression was highly cultivar-specific. In the three anthocyanin-containing cultivars, gene expression was coordinated and reflected at the metabolite level supporting a hypothesis that regulation of gene expression plays an essential role in the potato polyphenol production. We proposed that the altered sucrose flux induced by the drought stress is partly responsible for the changes in gene expression. This study provides information on key polyphenol biosynthetic and regulatory genes, which could be useful in the development of potato varieties with enhanced health and nutritional benefits. 相似文献
3.
The possibilities and limitations of supercritical fluid extraction of natural products of low, medium and high polarity under very high pressure and with polar modifiers has been investigated. The medicinal herbs marigold (Calendula officinalis), hawthorn (Crataegus sp.) and chamomile (Matricaria recutita) were used as models in this study. Extraction profiles and the spectra of extractable metabolites were recorded following extraction with mixtures of carbon dioxide:ethanol of varying proportions (0-20% ethanol) and at various pressures in the range 300-689 bar. Components were identified by HPLC-PAD-MS or GC-MS and quantified by HPLC or GC as appropriate. Extraction yields under the varying conditions depended to a large extent on the profiles of secondary metabolites present in the three drugs. Whereas the extractability of lipophilic compounds increased substantially at pressures above 300 bar, the yields of polyphenolic and glycosidic compounds remained low even at 689 bar and with 20% modifier in the extraction fluid. 相似文献
4.
Sesamol (3,4-methylenedioxyphenol), a monophenolic antioxidant in sesame iol, produced methemoglobin from hemoglobin A (oxyhemoglobin and deoxyhemoglobin) and from red cells. The activity of the compound was more extensive than the polyphenolic compounds. The profiles of the methemoglobin formation by the compound were compared with those by nitrite and hydroxylamine. The formation of methemoglobin from oxyhemoglobin by the compound was rather slowly progressed, but the amount of methemoglobin formed was proportional to the concentration of oxyhemoglobin even when the concentration of the compound was low. The sesamol-induced methemoglobin formation was influenced by inositol hexaphosphate, an allosteric effector of hemoglobin. Thus, the phosphate enhanced the transformation of oxyhemoglobin and inhibited the transformation of deoxyhemoglobin. 相似文献
5.
A systematic study of extraction efficiency of polyphenolic compounds from the most widespread Boraginaceae species was carried out. The optimal extraction was achieved with 50 % (V/V) methanol for phenolic acids and flavonoids, 0.2 % (V/V) HCl in 50 % (V/V) methanol for anthocyanins and pure water for flavan-3-ols. The distribution and diversity of polyphenolic compounds in plant material obtained from wild-growing Anchusa officinalis, Cynoglossum creticum Mill., Echium vulgare, Echium italicum, and Onosma heterophylla Griseb. species from Macedonia was also assessed. These widespread Boraginaceae species contain phenolic acid derivatives, flavonoids, flavan-3-ols and anthocyanins and in total 31 of them were identified, from which 22 were first identified in the representative species, and 6,8-di-C-glucosides of apigenin and luteolin were identified for the first time in Boraginaceae. The profiles of polyphenolic compounds for each sample were obtained and their phytochemical profile established. The potential for further bioactivity studies of Anchusa officinalis and Cynoglossum creticum containing up to 24577.05 μg/g and 14304.15 μg/g of total polyphenols were assumed to be highest, followed by Echium vulgare (from 6382.61 to 14114.33 μg/g), Onosma heterophylla (9463.97 μg/g) and Echium (4108.14 μg/g). 相似文献
6.
Ladiwala AR Perchiacca JM Fishman ZS Bhattacharya M Hickey AM Domigan BG Dordick JS Tessier PM 《Biotechnology and bioengineering》2012,109(7):1869-1874
Protein aggregation is a common problem during the purification and formulation of therapeutic proteins. Here we report that polyphenolic disaccharides are unusually effective at preventing protein aggregation. We find that two polyphenolic glycosides-naringin and rutin-endow diverse proteins with the ability to unfold without aggregating when heated, as well as the ability to refold without aggregating when cooled at low glycoside concentrations (<5 mM). This extreme solubilizing activity is a synergistic combination of the glycone and aglycone moieties, as combinations of polyphenols and sugars fail to suppress aggregation. Moreover, the activity of polyphenolic disaccharides is remarkably specific since their monosaccharide counterparts (as well as other common excipients such as arginine, trehalose, and cyclodextrin) fail to prevent aggregation at similar concentrations (<25 mM). We expect that polyphenolic disaccharides will be valuable additives for enhancing the solubility of proteins in applications plagued by protein aggregation. 相似文献
7.
C.T. Kumarappan E. Thilagam Subhash C. Mandal 《Saudi Journal of Biological Sciences》2012,19(3):349-355
In the present study antioxidant activities by (1,1-diphenyl-2-picrylhydrazyl radical (DPPH), hydrogen peroxide, hydroxyl radical inhibition, hemolysis by hydrogen peroxide assay, reducing power and total antioxidant activities of polyphenolic extract of Ichnocarpus frutescens leaves were investigated. The flavonoids and total polyphenolic contents of the extract were also determined using standard methods. Phytochemical analyses revealed the presence of flavonoids, polyphenols, anthocyanins and simple phenolic acids. The results of antioxidant activities of polyphenol extract obtained by different in vitro methods were varied depending on the method used. Nevertheless, polyphenol extract showed significant inhibitory activities in all in vitro reactive oxygen species scavenging, might be attributed due to the high level of polyphenolic compound. Also, these various antioxidant activities were compared to α-tocopherol and l-ascorbic acid as reference antioxidant compounds. These findings provide evidence that the polyphenolic extract of I. frutescens is a natural source of antioxidant against oxidative damage. 相似文献
8.
Laura Serrano 《Hydrobiologia》1992,229(1):43-50
The origin of allochthonous soluble polyphenols in the water of the temporary ponds of Doñana National Park during flood and heavy rainfall was examined. Soil and litter samples were found to leach large quantities of polyphenolic compounds. The concentration of these compounds showed a significant correlation with the organic content of the soil samples, but indicated no correlation with the litter samples. Rain simulation tests proved that fresh vegetation leached soluble polyphenolic compounds. Mediterranean scrub (Cystaceae) from dry poor-nutrient soils yielded the highest concentration (being furthest away from the ponds), whereas wetland (Ericaceae) and littoral vegetation (Juncaceae and Cyperaceae) rated second and third, respectively. The potential of the surrounding vegetation to leach high concentrations of polyphenolic compounds suggests that this is the main source of the rapid polyphenolic enrichment of these ponds during periods of heavy rain. 相似文献
9.
Ficus carica L. is one of the oldest trees belonging to the mulberry family. It has both nutritional and medicinal benefits, as it is a source of potential bioactive compounds. Fig leaves were collected at four maturation stages, to evaluate the variation in polyphenolic compounds, antioxidant activity, and different pigments level. Polyphenols were identified using HPLC–DAD. Different pigments level, total phenolic contents, and radical scavenging activity were quantified. The HPLC analysis revealed the presence of fourteen polyphenolic compounds in the fig leaves. Quercetin-3-glucoside, caftaric acid, quercetin-3, 7-diglucoside, and coumaroyl-hexose were the major phenolic compounds. Kaempferol-3-O-sophorotrioside, cichoric acid and sinapic acid glucoside were identified for the first time. Significant quantitative changes were observed during maturation, particularly in polyphenolic compounds. There were significant changes in pigments level, total phenolic contents, and radical scavenging activity. It was observed that fig leaves are a rich source of polyphenolic compounds during all stages of maturation. 相似文献
10.
Alessio Innocenti Ilhami Gülçin Andrea Scozzafava Claudiu T. Supuran 《Bioorganic & medicinal chemistry letters》2010,20(17):5050-5053
A series of polyphenolic derivatives, including resveratrol, dobutamine, curcumin, catechin and silymarine were investigated for the inhibition of all the catalytically active mammalian isozymes of the metalloprotein carbonic anhydrase (CA, EC 4.2.1.1), that is, CA I–CA XV. These polyphenols effectively inhibited CAs, with KIs in the range of 380 nM–12.02 μM. The various isozymes showed quite diverse inhibition profiles with these compounds, which possess scaffolds not present in other investigated CA inhibitors (CAIs). These data may lead to drug design campaigns of effective CAIs possessing a diverse inhibition mechanism compared to sulfonamide/sulfamate inhibitors, based on such less investigated scaffolds. 相似文献
11.
Antimutagenic properties of a polyphenol-enriched extract derived from sesame-seed perisperm 总被引:1,自引:0,他引:1
A polyphenolic mixture derived from sesame-seed perisperm (SSP) strongly reduced the mutagenicity of hydrogen peroxide (H(2)O(2)), sodium azide (NaN(3)), and benzo[a]pyrene (BaP) in strains TA100 and/or TA98 of Salmonella typhimurium. It exhibited desmutagenic activity against H(2)O(2), BaP in TA98 and/or TA100 and biomutagenic activity (apparently by affecting the DNA-repair system) against NaN(3) in strain TA100. According to in vitro experiments the polyphenolic mixture inhibited the activity of the CYP1A1 (EROD) enzyme responsible for the activation of BaP in the Ames' test, as well as that of the cytosolic enzyme GST. A cytosolic fraction from liver of male Wistar rats treated with either 20% SSP in the food, or 3mg or 6 mg of polyphenolic mixture/20 g food/day for a time period of 8 weeks reduced the mutagenic potential of BaP in strains TA100 and TA98, with the cytosolic fraction from rats treated with SSP causing the strongest reduction. Furthermore, a microsomal fraction from the 20% SSP-treated rats inhibited the mutagenicity of BaP in strains TA100 (26.3%) and TA98 (23%). In contrast, a microsomal fraction from rats treated with 3mg of polyphenolic mixture stimulated the mutagenicity of BaP in TA100 but reduced it in TA98, while for the microsomal fraction from rats treated with 6 mg of polyphenolic mixture, these effects on TA100 and TA98 were reversed. 相似文献
12.
Usha Singh Ajit Kumar Rajesh Sinha Sushma Manral Shvetambri Arora Sant Ram Rakesh K. Mishra Prachi Gupta Surendra K. Bansal Ashok K. Prasad Shyam Biswal Virinder S. Parmar Hanumantharao G. Raj 《Chemico-biological interactions》2010,185(3):263-270
Polyphenols, coumarin (1,2-benzopyrone) and chromone (1,4-benzopyrone), are naturally occurring constituent of variety of plant species. They have attracted immense interest because of their diverse pharmacological activities. Not much was known about biological activities of acetyl derivative (polyphenolic acetates) of parent polyphenols. In previous investigations, we have conclusively established calreticulin transacetylase catalyzed activation of endothelial nitric oxide synthase (eNOS) by polyphenolic acetates. In the present work, calreticulin transacetylase of human peripheral blood mononuclear cells was characterized with respect to specificity for various polyphenolic acetates and its role in the activation of TNF-α induced nitric oxide synthase (iNOS). Peripheral blood mononuclear cells incubated with a model polyphenolic acetate, 7,8-diacetoxy-4-methylcoumarin (DAMC), along with l-arginine caused activation of NOS. The incubation of peripheral blood mononuclear cells with TNF-α and DAMC resulted in increased production of NO as compared to TNF-α alone. This increased NO production was attenuated by l-Nω-nitro-l-arginine methyl ester (l-NAME), a well known non-specific inhibitor of NOS, and 1400W (N-[3-(aminomethyl) benzyl] acetamidine), a specific inhibitor of human iNOS. These results substantiate the CRTAase catalyzed activation of iNOS. Further, expression of NOS isoforms by semi-quantitative PCR and real-time RT-PCR confirms the preponderance of iNOS in TNF-α treated peripheral blood mononuclear cells over the untreated one. It was also observed that polyphenolic acetates inhibit TNF-α mediated release of IL-6 from peripheral blood mononuclear cells. 相似文献
13.
Isolation of DNA from plants high in polyphenolics 总被引:27,自引:3,他引:24
DNA suitable for digestion with restriction endonucleases has been isolated fromTheobroma cacao, a plant high in polyphenolic compounds, and several other plant taxa using the method presented in this paper. The method
relies on concentrating nuclei away from most of the cytoplasmic components and inhibiting the formation of oxidized polyphenolic
compounds and their interaction with the DNA fraction in the remaining steps. 相似文献
14.
《Saudi Journal of Biological Sciences》2023,30(2):103553
The wealth of epidemiological evidence in the scientific world underscores the possibility that a plant-based diet can reduce the prevalence of common diseases such as diabetes, cardiovascular disease, cancer, and stroke. The therapeutic effects of plant sources are partly explained by phenolic secondary metabolites or polyphenolic compounds. Therefore, polyphenolic compounds, which are widely distributed in plants, are of great interest for the development of effective specific drugs with antioxidant and anti-inflammatory effects. Moreover, polyphenol compounds have no harmful effects due to their natural biocompatibility and safety. Numerous studies have highlighted the potential of some industrial food wastes from plant material processing, including apple peels and mashed potatoes, grape skins, tomato and carrot peels, pomegranate peels and seeds, and many others. These byproducts are considered low-cost sources of natural biological compounds, including antioxidants, which have beneficial effects on human health.The polyphenol complex of pomegranate peel (Punica granatum L.), which makes up half of the pomegranate fruit, has more pronounced antioxidant and anti-inflammatory properties than other parts. And the most important active components of pomegranate peel, which are found only in this plant, are punicalagin, followed by ellagic acid and gallic acid. It is known that these polyphenolic compounds of pomegranate peel have the most pronounced therapeutic effect. Several studies have shown the protective effect of ellagic acid, punicalagin, against oxidative stress damage caused by free radicals. The potential of pomegranate peel as an antioxidant and therapeutic component in various biological systems is high, according to scientific sources.However, despite extensive research in recent years, a review of sources has shown that there is insufficient evidence to support the therapeutic effects of polyphenolic compounds from pomegranate peels. The role of pomegranate peel polyphenolic compounds, including flavonoids, as antioxidants in various biological systems also requires further research. Of particular importance are the mechanisms by which antioxidants influence the cellular response against oxidative stress. The purpose of this review was to report our current knowledge of plant polyphenolic compounds and their classification, and to evaluate the potential of phenolic compounds from pomegranate peels with significant antioxidant and therapeutic effects. 相似文献
15.
Forty-two samples of Sideritis species (Sideritis scardica, Sideritis raeseri, Sideritis syriaca, Sideritis taurica and Sideritis lanata) from the Balkan Peninsula were evaluated for their polyphenolic profiles in order to establish a correlation between the taxonomy, geographical location and nature and content of phenolic compounds.Eight compounds were detected in all analyzed Sideritis samples: 5-caffeoylquinic acid, lavandulifolioside, verbascoside, isoscutellarein 7-O-allosyl(1→2)glucoside, hypolaetin 7-O-[6‴-O-acetyl]-allosyl(1→2)glucoside, isoscutellarein 7-O-[6‴-O-acetyl]-allosyl(1→2)glucoside, 3′-O-methylhypolaetin 7-O-[6‴-O-acetyl]-allosyl(1→2)glucoside and 4′-O-methylhypolaetin 7-O-[6‴-O-acetyl]-allosyl-(1→2)-[6″-O-acetyl]-glucoside). They present from 50 to 80% of total phenolic content in S. scardica, S. raeseri, S. syriaca and S. taurica and up to 90% in S. lanata and the similarity of their polyphenolic profiles implies that they are systematically very closely related.Based on their polyphenolic patterns, very good differentiation between the samples of S. lanata (sect. Hesiodia) and S. scardica, S. syriaca and S. raeseri (sect. Empedoclia) was observed, that is mainly due to the very high content of 5-cafeoylquinic acid and very low amount of phenylethanoid glycosides and flavonoid glycosides.The obtained results demonstrate considerable degree of similarity between S. scardica, S. raeseri and Bulgarian S. syriaca that give contribution to the dilemma that Bulgarian S. syriaca is very similar to Turkish S. taurica and suggest further verification of its taxonomic status. 相似文献
16.
The aim of the study was to determine changes incurred by polyphenolic compounds from selected fruits in the lipid phase of
the erythrocyte membrane, in liposomes formed of erythrocyte lipids and phosphatidylcholine liposomes. In particular, the
effect of extracts from apple, chokeberry, and strawberry on the red blood cell morphology, on packing order in the lipid
hydrophilic phase, on fluidity of the hydrophobic phase, as well as on the temperature of phase transition in DPPC liposomes
was studied. In the erythrocyte population, the proportions of echinocytes increased due to incorporation of polyphenolic
compounds. Fluorimetry with a laurdan probe indicated increased packing density in the hydrophilic phase of the membrane in
presence of polyphenolic extracts, the highest effect being observed for the apple extract. Using the fluorescence probes
DPH and TMA-DPH, no effect was noted inside the hydrophobic phase of the membrane, as the lipid bilayer fluidity was not modified.
The polyphenolic extracts slightly lowered the phase transition temperature of phosphatidylcholine liposomes. The studies
have shown that the phenolic compounds contained in the extracts incorporate into the outer region of the erythrocyte membrane,
affecting its shape and lipid packing order, which is reflected in the increasing number of echinocytes. The compounds also
penetrate the outer part of the external lipid layer of liposomes formed of natural and DPPC lipids, changing its packing
order. 相似文献
17.
18.
T. G. WATSON 《Journal of applied microbiology》1975,38(2):133-142
A birdproof sorghum cultivar studied, contained substances inhibitory to the growth and fermentation of Lactobacillus leichmannii and Saccharomyces cerevisiae . The bacterial inhibitor is located in the pericarp fraction and is probably of a polyphenolic nature. Inhibition is through the energy metabolism of the bacteria and is completely relieved by the tannin complexing agents PVP and Triton X205. The yeast inhibitor occurs only in malted sorghum and is not polyphenolic in nature. Inhibition may be relieved partially by prior conversion of malt suspensions before introduction of the yeast. 相似文献
19.
In general, epiphytes have detrimental effects on the growth of their basiphytes due to competition for light and nutrients. Therefore, basiphyte species must expend energy suppressing epiphytes. Some studies suggest that phlorotannins, i.e. brown algal polyphenols, prevent colonization by epiphytes, whereas others question their allelopathic function because there is not necessarily a negative correlation between epiphyte abundance and the phlorotannin content of the basiphyte algae. Various phlorotannin components are found in brown algal species, thus we hypothesized that the antifouling activities of polyphenolic compounds may differ and that the analysis of phlorotannin profiles could be useful for estimating their ecological functions. We surveyed the epiphyte richness in the apical portions of 373 thalli from 15 sargassacean species, demonstrating that the variation and abundance of epiphyte species differed remarkably among the basiphyte species. However, there was a weak negative correlation between the density and total phlorotannin content of the basiphyte algae in only one of the 18 epiphyte species. The interspecific differences in the phlorotannin profile were characterized by quantitative 1H nuclear magnetic resonance spectroscopy (qNMR), and four major groups were categorized based on cluster and principal component analyses of polyphenolic signals in the qNMR spectra. The epiphyte Neosiphonia harveyi was more abundant on Sargassum hemiphyllum, S. patens and S. piluliferum than on other basiphyte species, and these three species were similar according to the cluster analysis. These results suggest that some phlorotannin components may be more effective for antifouling; thus interspecific differences in the phlorotannin profile could affect the variation and abundance of epiphytes. 相似文献
20.
Despoina Lazarou Raphael Grougnet Athanasios Papadopoulos 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2007,634(1-2):163-171
A polyphenolic mixture derived from sesame-seed perisperm (SSP) strongly reduced the mutagenicity of hydrogen peroxide (H2O2), sodium azide (NaN3), and benzo[a]pyrene (BaP) in strains TA100 and/or TA98 of Salmonella typhimurium. It exhibited desmutagenic activity against H2O2, BaP in TA98 and/or TA100 and biomutagenic activity (apparently by affecting the DNA-repair system) against NaN3 in strain TA100. According to in vitro experiments the polyphenolic mixture inhibited the activity of the CYP1A1 (EROD) enzyme responsible for the activation of BaP in the Ames’ test, as well as that of the cytosolic enzyme GST.A cytosolic fraction from liver of male Wistar rats treated with either 20% SSP in the food, or 3 mg or 6 mg of polyphenolic mixture/20 g food/day for a time period of 8 weeks reduced the mutagenic potential of BaP in strains TA100 and TA98, with the cytosolic fraction from rats treated with SSP causing the strongest reduction. Furthermore, a microsomal fraction from the 20% SSP-treated rats inhibited the mutagenicity of BaP in strains TA100 (26.3%) and TA98 (23%). In contrast, a microsomal fraction from rats treated with 3 mg of polyphenolic mixture stimulated the mutagenicity of BaP in TA100 but reduced it in TA98, while for the microsomal fraction from rats treated with 6 mg of polyphenolic mixture, these effects on TA100 and TA98 were reversed. 相似文献