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1.
Summary The bark texture of Melia azedarach L. changes from smooth to furrowed as trees age. In trees that were cut down, those with smooth bark sprouted below the cut from suppressed buds; trees with thick, furrowed bark sprouted at the edge of the cut surface from adventitious buds. The trees that had thin, furrowed bark sprouted mainly at the edge of the cut from adventitious buds, but sometimes also from suppressed buds in cracks. The relationship between sprouting pattern and tree architecture are discussed. 相似文献
2.
为探究小檗科植物八角莲组织培养的器官发生方式,该研究以八角莲离体叶片、叶柄在MS培养基上诱导产生的愈伤组织、不定芽、不定根为对象,用连续石蜡切片技术分析八角莲组织培养的器官发生途径。结果表明:八角莲愈伤组织形成的解剖学特征是靠近表皮的薄壁细胞经激素刺激恢复分裂能力,继续培养形成拟分生组织。拟分生组织可形成许多分化中心。通过对八角莲组织培养产生的不定芽细胞组织学观察发现芽原基起源于愈伤组织外侧的几层薄壁细胞,芽原基背离愈伤组织中央生长形成不定芽,故八角莲脱分化形成的芽起源方式为外起源。而八角莲的根原基起源于组织深处髓部薄壁细胞和部分维管形成层细胞,进而形成类似球形或楔形并朝韧皮部突起的根原基轮廓,根原基继续发育会突破表皮生成不定根,起源方式为内起源。八角莲离体再生途径为器官发生型,在组培苗生长过程中先诱导形成不定芽,再诱导形成不定根,在愈伤组织上形成维管组织将不定芽和不定根连接成完整植株。 相似文献
3.
Resting vegetative buds of Picea abies collected from phytotron-grown rooted cuttings of 24-year-old trees or a 12-year-old hedge were tested for their capacity to form adventitious buds after various cytokinin treatments. The most effective method for obtaining a high yield of adventitious buds within 8 weeks was to pulse treat the buds in 250 M BA for 3 h and then culture them on medium containing 5 M each of BA and kinetin for 1 week. The developmental pattern for adventitious bud production, with the formation of 10 to 20 adventitious buds per bud, was similar for all tested genotypes, although the number of buds giving rise to adventitious buds varied significantly. The capability of some clones to form adventitious buds was correlated to endogenous cytokinin content. The clone which contained most endogenous cytokinin in its resting bud had the highest potential for adventitious bud formation. 相似文献
4.
W. H. Weidlich 《American journal of botany》1976,63(10):1365-1379
The anatomy and organization of the stem vascular system was analyzed in representative taxa of Nymphaea (subgenera Anecphya, Lotos, and Brachyceras). The stem vascular system consists of a series of concentric axial stem bundles from which traces to lateral organs depart. At the node each leaf is supplied with a median and two lateral leaf traces. At the same level a root trace supplies vascular tissue to adventitious roots borne on the leaf base. Flowers and vegetative buds occupy leaf sites in the genetic spiral and in the parastichies seen on the stem exterior. Certain leaves have flowers related to them spatially and by vascular association. Flowers (and similarly vegetative buds) are vascularized by a peduncle trace that arises from a peduncle fusion bundle located in the pith. The peduncle fusion bundle is formed by the fusion of vascular tissue derived from axial stem bundles that supply traces to certain leaves. The organization of the vascular system in the investigated taxa of Nymphaea is unique to angiosperms but similar to other subgenera of Nymphaea. 相似文献
5.
Summary Microcuttings ofFraxinus excelsior, sampled from adult trees during the period of cell cycle blockage of bud in G0–1, developed long rejuvenated sprouts on the Woody Plant Medium (WPM) with benzylaminopurine (BAP) (4.0 mg/l) and indolebutyric acid (IBA) (0.03 mg/l). These sprouts had the ability to enter a resting period, building dormant-like buds when maintained on the original WPM. Sprouts developed from subcultures also entered a resting period without any transfer. Comparison of in nature buds in active growth and dormancy with buds of growing sprouts and in vitro dormant-like buds revealed similarity in behaviour at the shoot apical level. In particular, in dormant-like buds in a constant environment, shoot apical functioning was suppressed while the cell cycle of the shoot apex was blocked at the G0–1 phase, like in nature dormant buds.Abbreviations a.u.
arbitrary unit
- BAP
benzylaminopurine
- CF
cumulative frequency
- d1, d2
diameters of the shoot apex
- IBA
indolebutyric acid
- Pn
last opposite primordia of range n
- WPM
woody plant medium 相似文献
6.
Tree and stand level variables affecting the species richness, cover and composition of epiphytic lichens on temperate broad-leaved
trees (Fraxinus excelsior, Quercus robur, Tilia cordata, Ulmus glabra, and U. laevis) were analysed in floodplain forest stands in Estonia. The effect of tree species, substrate characteristics, and stand and
regional variables were tested by partial canonical correspondence analysis (pCCA) and by general linear mixed models (GLMM).
The most pronounced factors affecting the species richness, cover and composition of epiphytic lichens are acidity of tree
bark, bryophyte cover and circumference of tree stems. Stand level characteristics have less effects on the species richness
of epiphytic lichens, however, lichen cover and composition was influenced by stand age and light availability. The boreo-nemoral
floodplain forests represent valuable habitats for epiphytic lichens. As substrate-related factors influence the species diversity
of lichens on temperate broad-leaved trees differently, it is important to consider the effect of each tree species in biodiversity
and conservation studies of lichens.
Nomenclature Randlane et al. (2007) for lichens; Leht (2007) for vascular plants. 相似文献
7.
Damage to the buds of the common ash (Fraxinus excelsior L.) by larvae of the ash bud moth Prays fraxinella Bjerk. was investigated in trees growing in the presence, or absence, of an adjacent ditch. The presence of a ditch was correlated with increased damage levels due to bud moth. Saplings were used in an experiment to compare the effects of artificial drought and root damage with those of ditches. Both root damage and drought elicited analogous responses to those observed in trees growing near a ditch, being associated with elevated levels of bud moth exploitation and reduced growth of shoots. There exists a documented link between ditches, root disturbance, and the dieback of hedgerow ash trees in Great Britain; the response of P. fraxinella to environmental disturbances associated with dieback is hypothesised to be evidence of the possible role of moth damage as a causal factor in dieback. 相似文献
8.
We tested which factors explain the distribution pattern of the epiphytic moss Neckera pennata on three spatial scales using the framework of generalized linear models. First, we tested which factors explained its occurrence in forest stands in a 2500 ha landscape. At this scale, we also tested the effect of the historic landscape structure. We recorded its occurrence in all suitable stands. The occurrence probability increased with increasing present quantity of Acer platanoides, and with increasing present and past quantity of Fraxinus excelsior. The probability also increased with increasing connectivity to occupied stands. However, the connectivity to stands present in 1977 (recorded from infra‐red aerial photographs) explained more of the variation. This suggests that the regional metapopulation size of N. pennata has decreased during the past decades, and that its present distribution pattern reflects the age of the remaining stands, and the distribution of past dispersal sources in the landscape. Second, we tested which factors explained the occurrence and abundance on individual trees in three forest stands. Neckera pennata mainly occurred on Acer and Fraxinus stems. The most important variable in explaining occurrence probability was connectivity to surrounding occupied trees, which probably reflects the restricted dispersal range in this species. The abundance on occupied trees was also explained by this variable. The occurrence probability and abundance also increased with increasing tree diameter, probably reflecting the time that a tree has been available for colonization and the time since colonization, respectively. The occurrence probability and abundance furthermore decreased on strongly leaning (and deteriorating) trees. The occurrence probability increased with increasing bark roughness, probably reflecting increasing suitability regarding bark chemistry and moisture. Third, we tested its vertical distribution on occupied trees. The main distribution was below 1.6 m. 相似文献
9.
Primordial shoot explants excised from buds of one Larix decidua tree, about 30 years old, produced more adventitious buds, elongating into shoots, when grown on half strength Litvay medium than when grown on other basal media. Thidiazuron and N6-benzyladenine (BA) were equally effective in adventitious bud induction. In a comparative study of 30-year-old L. decidua, L. leptolepis, L. eurolepis, and L. laricina trees, explants from L. eurolepis and L. decidua produced a high number of cultures with adventitious buds that elongated into shoots; those from L. leptolepis were less productive, and those from L. laricina failed to form adventitious buds. The highest response was obtained with material collected in August and September, and in March and April; the lowest response occurred in explants from the October collection. 相似文献
10.
Matthias Dolek Anja Freese-Hager Adi Geyer Emilio Balletto Simona Bonelli 《Journal of Insect Conservation》2013,17(2):357-366
Globally, Euphydryas maturna is a polyphagous butterfly species. At our study sites in Italy and Germany however, the plant used for egg-laying was almost exclusively Fraxinus excelsior. Nevertheless, in Germany, two egg-batches were found on Ligustrum vulgare and one on Viburnum opulus. Females lay their egg-batches at low heights and mostly on small trees (but not <1 m). At the end of the flight season, small egg-batches are laid and we conclude that these are late batches laid by “old females”. Egg-batches and pre-diapause larval webs are often clustered on certain trees and even leaves. This was shown to be a result of female behaviour and not of habitat quality, since the trees chosen were different in subsequent years. Individual females may return for laying further egg-batches to the place of the first egg-deposition and other females follow, since the survival of pre-diapause larvae is higher when more larval webs are on one tree. A further possible advantage during hibernation is discussed. In Italy, post-hibernation larvae form two distinct cohorts: larvae feeding on herbaceous plants (Plantago major, P. minor, Veronica hederifolia), and larvae feeding on Fraxinus excelsior. Although the latter group starts feeding later and pupates later, it reaches the same pupation weight. At our German site, post-hibernation larvae were found almost exclusively on F. excelsior. In fact, we observed larvae searching for food on F. excelsior while the buds of this tree were still closed. We also found larvae searching for food in the herbal layer without finding suitable plants. In some areas, larvae have to wait for the buds of the ash tree to open. Our data suggest that conservation strategies for E. maturna must be site specific according to food plant use of pre- and post-hibernation larvae and habitat type. 相似文献
11.
Summary Explants derived from adventitious buds, rhizomes, stems, and leaves of a medicinal plant, Polygonatum cyrtonema, were studied for plantlet regeneration, and only adventitious bud explants were able to be regenerated into plantlets. Regeneration
was also accompanied by the formation of rhizome-like tissue, the medicinal portion of the plant. The optimum hormone combination
for plantlet regenertion was 4.44 μM benzyladenine plus 2.26 μM 2,4-dichlorophenoxyacetic acid, at which new adventitious buds were obtained from 96.6% of the adventitious bud explants,
with an average of 5.2 buds per explant. The best medium for root induction was half-strength Murashige and Skoog medium with
4.57 μM α-naphthaleneacetic acid, as 92% of regenerated buds rooted. Regenerated plantlets were successfully transferred to a greenhouse
with 86% survival. Histological observation indicated that new adventitious buds originated from the superficial meristematic
cell cluster of the granular callus induced from adventitious bud explants via organogenesis. 相似文献
12.
Resting vegetative buds of Norway spruce, Picea abies (L.) Karst., were induced to form adventitious bud primordia when cultured on medium -containing cytokinin. After transfer of the induced buds to medium lacking cytokinin, adventitious buds developed. The adventitious buds arose from meristems formed de novo in the needle primordia. No differences were found in the ability to form adventitious buds among buds collected from trees ranging from 5–50 years old. 相似文献
13.
Peter Del Tredici 《American journal of botany》1992,79(5):522-530
This study describes the origin and early development of a distinct organ of clonal regeneration in Ginkgo biloba, the basal chichi. These aggregates of suppressed shoot buds originate from superficial meristems located in the cotyledonary axils of all Ginkgo seedlings as part of their normal ontogeny. Within 6 wk of germination these buds become embedded in the cortex of the stem, and their subsequent growth and development occurs below the surface of the bark. When stimulated by some traumatic event that damages the seedling axis, one of these embedded cotyledonary buds usually grows down from the trunk to form a woody, rhizomelike basal chichi which, under appropriate conditions, is capable of generating both aerial shoots and adventitious roots. Vegetative regeneration by means of basal chichi has not only contributed to the long-term persistence of G. biloba in the forests of China, but may also have played a role in the remarkable survival of the genus since the Cretaceous. 相似文献
14.
The flowering patterns of Ulmus pumila and Fraxinus excelsior were studied during the 1990 growing season in order to investigate their pollen dispersion curves. The use of airborne pollen records as predictors of flowering of allergenic trees was evaluated. A sampling method to describe quantitative flowering phenophases was applied. A Burkard trap recorded airborne pollen in the city of Mar del Plata. The phenological sampling method showed that floral phenophase development is not always synchronous between trees. The variability registered was larger for F. excelsior than for U. pumila. A delay between flowering and the airborne pollen recorded was not noticed for Ulmus. The greatest amount of Ulmus pollen came from the local vegetation, mainly from U. pumila. Fraxinus pollen was recorded for a longer period after the F. excelsior flowering season the ended. Fraxinus pollen income came from F. excelsior but also from F. americana and F. excelsior var. aurea that flower later. An immediate decrease in airborne concentration was noticed for both pollen types following rain. When relatively strong rainfall was registered a decrease in the number of open flowers occurred only in F. excelsior. 相似文献
15.
P. Guicherd J. P. Peltier E. Gout R. Bligny G. Marigo 《Trees - Structure and Function》1997,11(3):155-161
In leaves of Fraxinus excelsior L., malate and mannitol were characterized by 13C NMR spectroscopy and enzymatic specific assays as the major constituents of a soluble carbon fraction involved in an osmotic
adjustment. During a summer drought where predawn leaf water potential of adult trees growing in a mesoxerophilic stand fell
to – 4 MPa in August, malate and mannitol leaf contents increased by a factor of 1.8 and 2.2 respectively, compared to control
trees growing on a flood plain. This drought stress led to concentrations as high as 280 mM and 600 mM for mannitol and malate,
respectively. The effects of gradually developing water deficit were also studied in a semi-controlled environment in 3-year-old
seedlings. When predawn leaf water potential reached -6 MPa, leaves displayed a low turgor pressure but stomatal conductance
was still measurable. Malate and mannitol were also the main osmoticum involved. After rewatering, gas exchange capacities
were largely restored. Altogether, these results show that the strong water-stress tolerance of Fraxinus excelsior is in part related to an accumulation of malate and mannitol.
Received: 3 January 1996 / Accepted: 19 March 1996 相似文献
16.
William Sanders 《American journal of botany》1989,76(5):666-678
The thallus of the lichen Ramalina menziesii Tayl. is comprised of subunits which resemble planar nets. The nets arise upon older nets as buds which develop perforations and expand. Microphotographic study of thallus development in the field shows several features which differ from a recently proposed model of development for this species. New perforations develop continually in new tissue produced at the apical margin of the net. The apical margin is inrolled, and may furcate. This type of branching is developmentally distinct from the formation of new nets from buds borne upon expanded parts of the net. Anatomical study of net apices appears to support assertions that programmed tissue necrosis contributes to perforation formation at sites where gaps are present in the algal layer. 相似文献
17.
Peter Marmann Renate Wendler Peter Millard H. Heilmeier 《Trees - Structure and Function》1997,11(5):298-305
Storage and remobilization of nitrogen (N) were studied in ash trees (Fraxinus excelsior) under both field and greenhouse conditions. Experiments in the greenhouse providing 15N labelled fertilizer to the trees showed that the major quantity of N remobilized during subsequent spring was from the roots,
and only a small amount from the stem. This corresponded with a loss of soluble N (proteins and low-molecular-weight compounds)
from both roots and stem. On the two field sites, which differed in water availability, there was a decrease in bark N content
during leaf growth, but on the dry site net N export from the bark was sustained throughout the whole vegetation period. Remobilized
N was derived from soluble proteins and low-molecular-weight compounds on the moist site, which was demonstrated by the seasonal
dynamics of a 56 kDa polypeptide in bark and wood. On the dry site, lower contents of soluble proteins were associated with
smaller amounts of N remobilized compared to the moist site. Uptake studies of 15N labelled fertilizer indicated a higher contribution of current uptake to leaf N increment during spring at the dry site
compared to the moist site. Differential N availability during the season had a decisive effect on the nitrogen storage dynamics
at the two sites. Thus the influence of current N supply on N remobilization and storage as found in the greenhouse-grown
plants could be verified under field conditions.
Received: 28 July 1995 / Accepted: 17 July 1996 相似文献
18.
Summary Concentrations of AMP, ADP, ATP, and inorganic phosphate (Pi) were determined in buds of five deciduous tree species (Acer pseudoplatanus, Alnus glutinosa, Fagus sylvatica, Fraxinus excelsior, Quercus robur) during spring reactivation from February to the middle of May. In closed buds of diffuse-porous wood trees (Acer, Alnus, Fagus), the content of adenine nucleotides (AdN) increased temporarily between the middle of February and the middle of March. The main increase of AdN concentration appeared either when buds became swollen (Fraxinus, Fagus, Quercus), or at the time of bud-break (Acer, Alnus). Pi content in general decreased during the course of reactivation. It was almost zero in buds of Quercus at bud-break and afterwards, but in Fraxinus Pi concentration rose when bud-break took place. The extremely low AdN content in Quercus buds is contrasted by a steep increase in AdN content in Fraxinus following bud-break. The decrease of AdN content in emerging leaves of Quercus and Fagus could be related to the high age of these trees.Abbreviations
AdN
adenine nucleotide(s)
-
DTT
dithiothreitol
-
EDTA
ethylenediaminetetraacetic acid
-
PEP
phosphoenolpyruvate
-
Pi
orthophosphate
Supported by Deutsche Forschungsgemeinschaft and by Bundesministerium für Forschung und Technologie, Federal Republic of Germany. 相似文献
19.
G. Jeandroz F. Faivre-Rampant A. Pugin J. Bousquet A. Bervillé 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,91(6-7):885-892
The ribosomal DNA repeat units of two closely related species of the genus Fraxinus, F. excelsior and F. oxyphylla, were characterized. The physical maps were constructed from DNA digested with BamHI, EcoRI, EcoRV and SacI, and hybridized with three heterologous probes. The presence or the absence of an EcoRV restriction site in the 18s RNA gene characterizes two ribosomal DNA unit types found in both species and which coexist in all individuals. A third unit type appeared unique to all individuals of F. oxyphylla. It carries an EcoRI site in the intergenic spacer. Each type of unit displayed length variations. The rDNA unit length of F. excelsior and F. oxyphylla was determined with EcoRV restriction. It varied between 11kb and 14.5kb in F. excelsior and between 11.8kb to 13.8kb in F. oxyphylla. Using SacI restriction, at least ten spacer length variants were observed in F. excelsior, for which a detailed analysis was conducted. Each individual carries 2–4 length variants which vary by a 0.3-kb step multiple. This length variation was assigned to the intergenic spacer. By using the entire rDNA unit of flax as probe in combination with EcoRI restriction, each species can be unambiguously discriminated. The species-specific banding pattern was used to compare trees from a zone of sympatry between the two species. In some cases, a conflicting classification was obtained from morphological analysis and the use of the species-specific rDNA polymorphism. Implications for the genetic management of both species are discussed. 相似文献
20.
Yoshihito Hongo 《Journal of Ethology》2006,24(3):201-204
The Japanese horned beetle, Trypoxylus dichotomus septentrionalis, usually aggregates at the wounds caused by boring insects on Quercus oak trees to feed on exuding sap. Here, a unique behavior of the beetle, which enables it to independently obtain sap without any activities of other insects, is reported. Beetles carved intact bark of Fraxinus griffithii, where no sap was exuding, using clypeus. As a result of carving behavior, bark was stripped, and sap exuded from these scars temporarily, which beetles immediately began to lick. A digital video image relating to the article is available at (). 相似文献