黄瓜香等对调整腹水瘤小鼠肠道菌群并延长荷瘤生存时间的影响

目的研究黄瓜香等在调节肠道菌群和改善荷瘤小鼠生存质量,提高生存率方面的作用。方法用肝癌腹水瘤H22细胞株注射小鼠造荷瘤小鼠模型,然后口服黄瓜香提取液治疗。观察治疗前后菌群变化、腹水量、荷瘤生存时间等。结果中药组与阴性对照组比较,肠道菌群趋于平衡、腹水出现时间延迟、腹水量降低、荷瘤生存时间延长。结论黄瓜香等能调节小鼠肠道菌群,改善荷瘤小鼠生存质量、提高生存率。     

黄瓜香等山野菜对糖尿病大鼠脂质代谢及肠道正常菌群的影响

目的观察黄瓜香等对糖尿病大鼠脂质代谢的影响,并进一步探讨其调脂机制。方法选取糖尿病大鼠灌胃给予黄瓜香提取液120mg/（kg.d）,共8周,检测其空腹血脂变化,及其肠道菌群变化。结果（1）经黄瓜香提取物治疗后,治疗组较糖尿病组,甘油三脂、低密度脂蛋白和胆固醇明显降低（P〈0.01）;（2）糖尿病组肠道菌群较正常组有明显变化（P〈0.01）,尤其双歧杆菌、乳杆菌等明显减少,而治疗后肠道菌群得到调整。结论黄瓜香提取物可能通过扶植肠道菌群生长繁殖,对脂质代谢发挥作用,从而达到其调脂目的。     

黄瓜香水、醇提取液对肠道菌群的调整作用的比较

目的研究不同溶剂提取黄瓜香有效成分的作用效果,以期能更好提高药物的利用度。方法用盐酸林可霉素制造小鼠菌群失调模型,然后分别用黄瓜香的水、醇提取物治疗,比较治疗效果。结果醇提取物和水提取物均能调整肠道菌群平衡,与自然恢复组相比差异有统计学意义(P0.05),且醇提取物组效果要优于水提取物组(P0.05)。结论黄瓜香醇提取物是理想的微生态调节剂。     

蒲公英提取液调整小鼠肠道菌群并延长荷瘤生存时间的研究

目的研究蒲公英在调节肠道菌群和改善荷瘤小鼠生存质量提高生存率方面的作用。方法用肝癌腹水瘤H22细胞株注射小鼠造荷瘤小鼠模型,然后口服蒲公英提取液治疗。观察治疗前后菌群变化、腹水量和荷瘤生存时间等。结果蒲公英治疗组与阴性对照组比较,肠道菌群趋于平衡、腹水出现时间延迟、腹水量降低和荷瘤生存时间延长。结论蒲公英提取液能调节小鼠肠道菌群,改善荷瘤小鼠生存质量、提高生存率。     

黄瓜香联合顺铂治疗小鼠H22肝癌的研究

目的 观察黄瓜香联合顺铂对小鼠H22肝癌移植瘤生长的抑制作用.方法 将40只接种H22肝癌细胞的昆明小鼠随机分为4组,其腹腔分别注射生理盐水(对照组);黄瓜香组;顺铂组;黄瓜香+顺铂组(联合治疗组),观察小鼠的毒副反应及生存质量.实验19 d后,处死全部小鼠,剥离皮下肿瘤,称小鼠肿瘤重量,计算抑瘤率.结果 黄瓜香组的H22肝癌平均瘤重为(1.26 ±0.19)g,明显低于对照组的(1.89 ±0.56)g(P ＜0.01).联合治疗组的平均瘤重为(0.57 ±0.42)g,均明显低于黄瓜香组(P＜0.01)和顺铂组(P＜0.01);其抑瘤率达69.8％,明显高于黄瓜香组(x2=16.9875,P＜0.01)和顺铂组(x2=5.0602,P＜0.05).联合治疗组小鼠的毒副反应明显低于顺铂组,生存质量好于顺铂组;黄瓜香组与联合治疗组都能调节肠道菌群,扶植肠道中有益菌(乳酸杆菌和双歧杆菌)生长,抑制大肠埃希菌生长,与对照组比较差异具有统计学意义(P＜0.01).结论 黄瓜香与顺铂合用对小鼠H22肝癌移植瘤的生长具有协同抑制作用,能降低顺铂毒副反应,提高小鼠的生存质量.     

黄瓜香等中草药对寻常型痤疮治疗效果的观察

目的应用黄瓜香等中草药作为微生态调节剂治疗青少年寻常型痤疮。方法通过比较治疗前后痤疮患者面部皮肤痤疮丙酸杆菌和表皮葡萄球菌数量以及面部皮疹的数量来判断黄瓜香等中草药治疗痤疮的疗效。结果黄瓜香抑制了痤疮丙酸杆菌和表皮葡萄球菌生长（P〈0．05）;减少了皮疹数量（P〈0．05）,使皮疹不再出现。结论黄瓜香等能够治疗青少年寻常型痤疮。     

分叉双歧杆菌对小鼠腹水瘤的抑制作用

本文观察了分叉双歧杆菌对小鼠腹腔移植的淋巴细胞腹水瘤(SRS)的抑制作用。结果发现,分叉双歧杆菌在SRS瘤细胞移植前或移植后应用,均能明显抑制瘤细胞的生长,特别在移植后应用,抗瘤效果更显著。主要表现为荷瘤小鼠存活时间延长、存活率提高。将该菌预先进行处理或不处理后加入体外培养的SRS瘤细胞中,发现该菌对离体的瘤细胞生长也有直接抑制作用。     

微生态调节剂黄瓜香治疗细菌性阴道炎疗效观察

目的 观察微生态调节剂黄瓜香治疗细菌性阴道炎的疗效.方法 用黄瓜香提取液治疗细菌性阴道炎,治疗前后分别进行测阴道分泌物pH、做线索细胞和胺试验检查,进行总有效率评定.结果 经2种药物治疗后所有患者临床症状均明显减轻,治疗前后自身比较差异有显著性(P<0.01); 83%以上的患者呈现线索细胞转(-)、胺试验转(-) ,超过一半的患者阴道分泌物pH低于4.5;临床治疗总有效率分别为76.66%和79.4%%.2种药物组间比较治疗效果差异无显著性(P>0.05).结论 阴道补充黄瓜香与使用甲硝唑治疗产生了相似的效果,有可能成为治疗细菌性阴道炎的新药.     

胃癌患者化疗前后口腔菌群与肠道菌群变化特征

目的 研究胃癌患者化疗前后口腔菌群与肠道菌群的变化特征,为该类患者的治疗提供参考.方法 选取2017年2月至2020年2月于我院接受治疗的96例胃癌患者为观察组,另外选取同期我院健康体检者90例为对照组,收集两组对象唾液样本和粪便样本,其中观察组患者需收集化疗前及化疗后两个时间点的样本.采用16SrRNA基因高通量测序...     

化疗安口服液对白血病患者化疗后免疫功能及肠道菌群影响的研究

目的 化疗安口服液扶植肠道正常菌群生长,可减轻化疗药副作用,提高白血病化疗患者免疫力。方法 按常规方法筛选出具有扶植肠道正常菌群生长,恢复免疫功能的最佳中药;将选择病例随机分为化疗安口服液治疗组、常规化疗组,观察其化疗前、化疗后10d肠道常驻菌（肠杆菌、肠球菌、双歧杆菌和乳杆菌）指标,测定T细胞亚群（CD3、CD4、CD8和CD4／CD8）,免疫球蛋白（IgG、IgM和IgA）指标。结果 各项指标同单纯化疗组相比,差异均有极显著性（P〈0．01）。结论 中药化疗安口服液具有促进双歧杆菌与乳酸杆菌等有益于机体的优势菌的生长,抑制肠杆菌和肠球菌生长的作用,并具有减少化疗患者合并症,提高免疫功能。     

纳米中药对S180荷瘤小鼠肠道菌群影响的研究及其防治作用

目的研究纳米中药对S180荷瘤小鼠肠道菌群的影响及其抑制作用。方法将S180荷瘤瘤株以2×105/(0.2 m l.鼠)用注射器接种于小鼠右前肢腋下,建立实体瘤模型,肿瘤发生率为100%。观察小鼠的一般状态,用梯度稀释法和培养法测定小鼠4种肠道正常菌群,即乳酸杆菌、双歧杆菌、肠球菌、肠杆菌。用电子天平称瘤重并计算瘤抑制率。病理行HE(苏木精-伊红)染色,在光学显微镜下观察。结果与模型组比较,纳米中药组双歧杆菌和乳酸杆菌数量明显升高,肠球菌和肠杆菌数量明显减少,肿瘤坏死因子数量增加,抑瘤率达64%,病理显示肿瘤组织间坏死灶明显,有大量的炎性细胞浸润。结论纳米中药提高机体免疫屏障功能,增强药物的靶向性,扶植机体正常菌群的生长,实现抗肿瘤的目的。     

双歧杆菌三联活菌胶囊对进展期胃癌术后化疗患者化疗副作用及肠道菌群的影响

目的 探讨口服双歧杆菌三联活菌胶囊（BTVC）对进展期胃癌术后化疗患者的化疗副作用及肠道菌群影响。方法 选取铜陵市人民医院2016年2月至2017年1月收治的根治性胃癌术后首次化疗患者为研究对象,随机分成奥沙利铂+替吉奥联合化疗方案组32例（对照组）,奥沙利铂+卡培他滨+BTVC联合治疗组26例（观察组）。检测两组患者肠道菌群变化及记录患者不良反应情况。结果 治疗后对照组中白细胞＜3×109/L、粒细胞＜2×109/L、血红蛋白＜110 g/L、血小板＜100×109/L、肝功能损害、周围神经炎、恶心/呕吐、腹泻及Ⅲ/Ⅳ级不良反应发生例数均多于观察组（P＜0.05）。治疗前两组患者肠道各菌群数量差异无统计学意义（P＞0.05）;治疗后对照组患者乳杆菌、双歧杆菌数量及杆/球比明显少于观察组（P＜0.01）,而肠球菌、大肠埃希菌数量明显多于观察组（P＜0.01）。治疗后对照组患者乳杆菌、双歧杆菌数量及杆/球比明显少于治疗前（P＜0.01）,而肠球菌、大肠埃希菌数量多于治疗前（P＜0.05）。治疗后观察组患者乳杆菌、双歧杆菌数量及杆/球比多于治疗前（P＜0.05）;而肠球菌、大肠埃希菌数量与治疗前差异无统计学意义（P＞0.05）。结论 BTVC对胃癌术后化疗副作用有预防作用,并可调节患者肠道菌群。     

Acanthopanax senticosus total flavonoids alleviate lipopolysaccharide-induced intestinal inflammation and modulate the gut microbiota in mice

Here, we study the therapeutic effect of Acanthopanax senticosus total flavonoids (ASTFs) using a mouse intestinal inflammation model. The inflammation model used in the present study was developed through lipopolysaccharide (LPS) treatment of mice. The experimental mice were divided into a control group, model group (10 mg/kg LPS), dexamethasone group (1 mg/kg DEX) and ASTF low-, medium- and high-dosage groups (200, 400 and 800 mg/kg, respectively). The morphological and structural changes in the ileum, jejunum and duodenum were observed using HE staining. The number of intestinal goblet cells (GCs) was calculated based on PAS staining. The contents of interleukin (IL)-1β, IL-6, prostaglandin E2 (PGE2) and tumor necrosis factor α (TNF-α) were determined using enzyme-linked immunosorbent assay (ELISA) and the related mRNA expression level were measured by RT-PCR. The protein expression levels of Toll-like receptor 4 (TLR4), MyD88, p65 and p-p65 were measured using Western blotting. In addition, the 16S rRNA sequences of bacterial taxa were amplified and analyzed to assess changes in the intestinal microbes of LPS-induced mice and also in response to regulation by ASTF. Following intervention with ASTF, different therapeutic effects were shown according to the various dosages tested, all of which resulted in improved intestinal morphology and an increased number of intestinal GCs, while the contents of IL-1β, IL-6, PGE2 and TNF-α and the related mRNA expression level were significantly reduced. The TLR4, MyD88 and p-p65/p-65 protein expression levels were also significantly reduced. In addition, 16S rRNA sequencing results show that LPS disrupts the structure of mouse gut microbes, though we observed that normal microbial status can be restored through ASTF intervention.     

两种方式接种肝癌模型的比较

目的观察直接注入法和瘤块种植法制作Wistar大鼠肝癌模型的差异。方法采用大鼠含有Walker-256肿瘤细胞的腹水离心洗涤后接种于另一组大鼠的后腿后外侧皮下,待肿瘤长到直径约为1．0em,取下肿瘤并切成1．0-2．0mm^3大小。然后将瘤块接种于15只大鼠的肝叶上;另一组（15只）按上述方式将癌性腹水接种于正常大鼠的肝叶上;两组均在第7天后采用CT和开腹后游标卡尺分别测量种植性肝癌的直径。结果腹水直接注射法与瘤块种植法的肿瘤成瘤率分别为：86．7％和80％,两者并不具有统计学意义（P〉0．05）。第7天时,直接注射法所形成肿瘤的直径大于瘤块种植法的肿瘤（P〈0．05）。但瘤块法所引起的腹腔转移的可能性要少于腹水直接种法。结论直接注射法和瘤块种植法制作大鼠肝癌模型均可以满足临床实验研究的需要,但直接注射法的成瘤时间短,腹腔转移可能性也大;瘤块种植法成瘤时间略长,但腹腔转移可能性少于直接法。     

A side effect of chlorthiamid and dichlobenil herbicides

2,6-Dichlorobenzamide (BAM) induced leaf margin chlorosis (LMC) on the leaves of kale seedlings and apple trees when applied to the roots. The leaf symptoms were similar to those sometimes seen after use of the herbicides chlorthiamid and dichlobenil. BAM was deposited mainly in the margin of the leaf to which BAM was transported via the transpiration stream. BAM appeared to be the causative agent of chlorosis although hydroxy derivatives of BAM were also present in the leaf. Factors possibly responsible for the variations in the occurrence and in the intensity of LMC are discussed.     

肠道菌群与疾病发生及中草药调节和治疗作用的研究概述

肠道正常菌群及微生态稳定对人体极具重要性,中草药对肠道菌群具有调节作用.本文综述了人体的肠道正常菌群与疾病产生和发展的关系,结合中草药对肠道菌群调节作用的研究,探讨中药调节肠道菌群与其发挥疾病防治效果之间的相关性,以期为中草药的临床应用及药理学研究提供一定的参考依据.     

Succession of microfungal flora on Rodgersia podophylla plants at the forest side of Cryptomeria plantation

Microfungal flora on aerial parts of a forest floor plant, Rodgersia podophylla, were studied at the forest side of a Japanese cedar plantation. From May to October, Nigrospora spp. were dominantly isolated from leaves, whereas Acremonium spp. and Fusarium spp. were dominant on stems, suggesting that the aerial part of the plants about 1 m height can offer two different habitats for these dominant fungi. In September and October, we could easily discern different types of tissue on the withering leaves, i.e., brown (necrotic lesion), yellowish (border tissue between brown and green areas), and green (healthy tissue). Nigrospora spp. and Pestalotiopsis spp. were continuously isolated on the brown area as well as on green and yellowish areas. Pathogenicity of N. sacchari and P. neglecta on potted plant leaves was confirmed by inoculation. From these, the fungi of these two genera seemed to have changed from quiescent to pathogenic with leaf senescence. Sporulations by fungi of the two genera were recognized on overwintered stems. These fungi may overwinter in stems that are slow to decompose, and seem to go over to the leaves in the following spring. Thus, they could be candidates for parasites that may play an important role in decomposition of the plant.