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R A Young 《The Journal of biological chemistry》1979,254(24):12725-12731
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Evidence for an internal promoter in the Escherichia coli threonine operon. 总被引:4,自引:1,他引:4 下载免费PDF全文
Auxotrophic mutants of Halobacterium volcanii generated by chemical mutagenesis were used to demonstrate a native genetic transfer system in this extremely halophilic member of the class Archaeobacteria. 相似文献
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Evidence of a secondary promoter for the guaA gene within the guaB gene was obtained by using lambdapguaA transducing phage. The technique is generally applicable to distinguish a promoter present within a bacterial deoxyribonucleic acid segment, which has replaced the lambda b2 region of transducing phage, from the phage pI promoter. 相似文献
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Nakata Kotoko; Kanehisa Minoru; Maizel Jacob V. Jr 《Bioinformatics (Oxford, England)》1988,4(3):367-371
We have previously developed a general method based on the statisticaltechnique of discriminant analysis to predict splice junctionsin eukaryotic mRNA sequences [Nakata, K., Kanehisa, M. and DeLisi,C. (1985) Nucleic Acids Res., 13, 53275340]. In orderto evaluate further applicability of this method, we now analyzethe promoter region of Escherichia coli sequences. The attributesused for discrimination include the accuracy of consensus sequencepatterns measured by the perceptron algorithm, the thermal stabilitymap, the base composition and the Calladine-Dickerson rulesfor helical twist angle, roll angle, torsion angle and propellertwist angle. When applied to selected E. coli sequences in theGenBank database, the method correctly identifies 75 % of thetrue promoter regions.
Received on May 15, 1987; accepted on April 17, 1988 相似文献
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J D Hopkins 《Journal of molecular biology》1974,87(4):715-724
The isolation and genetic characterization of a number of mutations that are located in the promoter region of the lac2 operon are described. These mutations have reduced levels of lac operon expression in a wild, type (crp+cya+) genetic background. Three of the mutations also have lower levels of lac operon expression than lacP+ in a crp?cya? genetic background, that is in the absence of the catabolite activator protein and 3′,5′-adenosine cyclic monophosphate. These three mutations are located nearest to the lac operator. They define a second essential site in the promoter region. 相似文献