共查询到20条相似文献,搜索用时 15 毫秒
1.
Transmembrane 22Na fluxes across bimolecular lipid membranes are measured under two different experimental conditions: (a) the pH is the same in the two bulk aqueous solutions on either side of the membrane while the concentrations of Na+ are different; (b) the concentrations of Na+ are identical but pH of the two solutions are different. In this latter case, the transport of Na+ occurs in the opposite direction to the difference of the proton concentration. In both cases, the electrical charge flux is negligible. A transport model is proposed to account for the experimental data. 相似文献
2.
Ouabain switches Na+ transport to Na+/Na+ equivalent exchange in normal and apoptotic lymphoid cells
A. A. Rubashkin 《Biophysics》2013,58(5):660-663
A theory of change of the ionic fluxes in the lymphoid cells in their transition from normal to apoptosis we have developed previously is applied to the analysis of Na+/Na+ exchange fluxes in human lymphoid cells U937 exposed to ouabain. We solve a system of equations describing changes in the intracellular concentrations of Na+, K+ and Cl?, membrane potential and cell volume. It is shown that the Na+ influx (I Na/Na) and output flux through the Na+/Na+ tract increased 4 times in 8 h after disconnecting Na+/K+-ATPase for normal cell U937. These fluxes increased 2.6 times for apoptotic cells. The value of I Na/Na after 8 h off pump by ouabain is 97% of the total Na+ input for both cell types. It is concluded that ouabain not only inhibits the Na+/K+-ATPase, but also increases Na+ exchange fluxes through the Na+/Na+ tract, thereby switching sodium transport across the membrane of lymphoid cells to Na+/Na+ equivalent exchange. 相似文献
3.
A mathematical model for amino acid uptake by membrane vesicles is described which includes two components, a Na+ dependent and a Na+ independent system. Uptake in the model is a function of both initial external Na+ and amino acid concentrations. Sodium dependence of amino acid transport in the model is manifested by changing affinity constants for amino acid uptake under different Na+ concentrations. The differing affinities for influx and efflux caused by increasing internal Na+ concentrations with time during transport incubations result in an “overshoot” for amino acid accumulation. For inwardly directed Na+ gradients, the model predicts the dependence of the occurrence of the overshoot on initial external substrate concentration and the dependence of the height of the overshoot on initial external Na+ concentration. This model has been used to describe experimental data on proline uptake by rat renal brushborder membrane vesicles. 相似文献
4.
Laura?J. Mares Alvaro Garcia Helge?H. Rasmussen Flemming Cornelius Yasser?A. Mahmmoud Joshua?R. Berlin Bogdan Lev Toby?W. Allen Ronald?J. Clarke 《Biophysical journal》2014,107(6):1352-1363
The charge-transporting activity of the Na+,K+-ATPase depends on its surrounding electric field. To isolate which steps of the enzyme’s reaction cycle involve charge movement, we have investigated the response of the voltage-sensitive fluorescent probe RH421 to interaction of the protein with BTEA (benzyltriethylammonium), which binds from the extracellular medium to the Na+,K+-ATPase’s transport sites in competition with Na+ and K+, but is not occluded within the protein. We find that only the occludable ions Na+, K+, Rb+, and Cs+ cause a drop in RH421 fluorescence. We conclude that RH421 detects intramembrane electric field strength changes arising from charge transport associated with conformational changes occluding the transported ions within the protein, not the electric fields of the bound ions themselves. This appears at first to conflict with electrophysiological studies suggesting extracellular Na+ or K+ binding in a high field access channel is a major electrogenic reaction of the Na+,K+-ATPase. All results can be explained consistently if ion occlusion involves local deformations in the lipid membrane surrounding the protein occurring simultaneously with conformational changes necessary for ion occlusion. The most likely origin of the RH421 fluorescence response is a change in membrane dipole potential caused by membrane deformation. 相似文献
5.
Laura J. Mares Alvaro Garcia Helge H. Rasmussen Flemming Cornelius Yasser A. Mahmmoud Joshua R. Berlin Bogdan Lev Toby W. Allen Ronald J. Clarke 《Biophysical journal》2014
The charge-transporting activity of the Na+,K+-ATPase depends on its surrounding electric field. To isolate which steps of the enzyme’s reaction cycle involve charge movement, we have investigated the response of the voltage-sensitive fluorescent probe RH421 to interaction of the protein with BTEA (benzyltriethylammonium), which binds from the extracellular medium to the Na+,K+-ATPase’s transport sites in competition with Na+ and K+, but is not occluded within the protein. We find that only the occludable ions Na+, K+, Rb+, and Cs+ cause a drop in RH421 fluorescence. We conclude that RH421 detects intramembrane electric field strength changes arising from charge transport associated with conformational changes occluding the transported ions within the protein, not the electric fields of the bound ions themselves. This appears at first to conflict with electrophysiological studies suggesting extracellular Na+ or K+ binding in a high field access channel is a major electrogenic reaction of the Na+,K+-ATPase. All results can be explained consistently if ion occlusion involves local deformations in the lipid membrane surrounding the protein occurring simultaneously with conformational changes necessary for ion occlusion. The most likely origin of the RH421 fluorescence response is a change in membrane dipole potential caused by membrane deformation. 相似文献
6.
Immunological assays and transport measurements in apical membrane vesicles revealed that the apical membrane of rat kidney cortex and medulla presents OCTN2 and OCTN3 proteins and transports L ‐[3H]‐carnitine in a Na+‐dependent and ‐independent manner. OCTN2 mediates the Na+/L ‐carnitine transport activity measured in medulla because (i) the transport showed the same characteristics as the cortical Na+/L ‐carnitine transporter and (ii) the medulla expressed OCTN2 mRNA and protein. The Na+‐independent L ‐carnitine transport activity appears to be mediated by both OCTN2 and OCTN3 since: (i) Na+‐independent L ‐carnitine uptake was inhibited by both, anti‐OCTN2 and anti‐OCTN3 antibodies, (ii) kinetics studies revealed the involvement of a high‐ and a low‐affinity transport systems, and (iii) Western and immunohistochemistry studies revealed that OCTN3 protein is located at the apical membrane of the kidney epithelia. The Na+‐independent L ‐carnitine uptake exhibited trans‐stimulation by intravesicular L ‐carnitine or betaine. This trans‐stimulation was inhibited by anti‐OCTN3 antibody, but not by anti‐OCTN2 antibody, indicating that OCTN3 can function as an L ‐carnitine/organic compound exchanger. This is the first report showing a functional apical OCTN2 in the renal medulla and a functional apical OCTN3 in both renal cortex and medulla. J. Cell. Physiol. 223: 451–459, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
7.
《Channels (Austin, Tex.)》2013,7(5):346-357
Na+-coupled phosphate cotransporters of the SLC34 gene family catalyze the movement of inorganic phosphate (Pi) across epithelia by using the free energy of the downhill electrochemical Na+ gradient across the luminal membrane. Electrogenic (NaPi-IIa/b) and electroneutral (NaPi-IIc) isoforms prefer divalent Pi and show strict Na+:Pi stoichiometries of 3:1 and 2:1, respectively. For electrogenic cotransport, one charge is translocated per transport cycle. When NaPi-IIa or NaPi-IIb are expressed in Xenopus oocytes, application of the Pi transport inhibitor phosphonoformic acid (PFA) blocks a leak current that is not detectable in the electroneutral isoform. In this review, we present the experimental evidence that this transport-independent leak originates from a Na+-dependent uniport carrier mode intrinsic to NaPi-IIa/b isoforms. Our findings, based on the characteristics of the PFA-inhibitable leak measured from wild-type and mutant constructs, can be incorporated into an alternating access class model in which the leak and cotransport modes are mutually exclusive and share common kinetic partial reactions. 相似文献
8.
B. Blazer-Yost M. Geheb A. Preston J. Handler M. Cox 《Biochimica et Biophysica Acta (BBA)/General Subjects》1982,719(1):158-161
Similar aldosterone-induced proteins have been demonstrated in two renal epithelia, the urinary bladder of the toad, Bufo marinus, and epithelia formed by cells of the A6 line derived from the kidney of the toad, Xenopus laevis. The proteins are induced along with the stimulation of Na+ transport but their synthesis is not dependent on Na+ transport per se. In view of the similar characteristics of the aldosterone-induced proteins in these two different epithelia, we suggest that they may have an important role in aldosterone-induced Na+ transport. 相似文献
9.
Teresita Padilla-Benavides María L. Roldán Isabel Larre David Flores-Benitez Nicolas Villegas-Sepúlveda Ruben G. Contreras Marcelino Cereijido Liora Shoshani 《Molecular biology of the cell》2010,21(13):2217-2225
The very existence of higher metazoans depends on the vectorial transport of substances across epithelia. A crucial element of this transport is the membrane enzyme Na+,K+-ATPase. Not only is this enzyme distributed in a polarized manner in a restricted domain of the plasma membrane but also it creates the ionic gradients that drive the net movement of glucose, amino acids, and ions across the entire epithelium. In a previous work, we have shown that Na+,K+-ATPase polarity depends on interactions between the β subunits of Na+,K+-ATPases located on neighboring cells and that these interactions anchor the entire enzyme at the borders of the intercellular space. In the present study, we used fluorescence resonance energy transfer and coprecipitation methods to demonstrate that these β subunits have sufficient proximity and affinity to permit a direct interaction, without requiring any additional extracellular molecules to span the distance. 相似文献
10.
The aim of this study was to clarify the mechanism of isotonic fluid transport in frog skin glands. Stationary ion secretion
by the glands was studied by measuring unidirectional fluxes of 24Na+, 42K+, and carrier-free 134Cs+ in paired frog skins bathed on both sides with Ringer's solution, and with 10−5
m noradrenaline on the inside and 10−4
m amiloride on the outside. At transepithelial thermodynamic equilibrium conditions, the 134Cs+ flux ratio, J
out
Cs/J
in
Cs, varied in seven pairs of preparations from 6 to 36. Since carrier-free 134Cs+ entering the cells is irreversibly trapped in the cellular compartment (Ussing & Lind, 1996), the transepithelial net flux
of 134Cs+ indicates that a paracellular flow of water is dragging 134Cs+ in the direction from the serosal- to outside solution. From the measured flux ratios it was calculated that the force driving
the secretory flux of Cs+ varied from 30 to 61 mV among preparations. In the same experiments unidirectional Na+ fluxes were measured as well, and it was found that also Na+ was subjected to secretion. The ratio of unidirectional Na+ fluxes, however, was significantly smaller than would be predicted if the two ions were both flowing along the paracellular
route dragged by the flow of water. This result indicates that Na+ and Cs+ do not take the same pathway through the glands. The flux ratio of unidirectional K+ fluxes indicated active secretion of K+. The time it takes for steady-state K+ fluxes to be established was significantly longer than that of the simultaneously measured Cs+ fluxes. These results allow the conclusion that — in addition to being transported between cells — K+ is submitted to active transport along a cellular pathway.Based on the recirculation theory, we propose a new model which
accounts for stationary Na+, K+, Cl− and water secretion under thermodynamic equilibrium conditions. The new features of the model, as compared to the classical
Silva-model for the shark-rectal gland, are: (i) the sodium pumps in the activated gland transport Na+ into the lateral intercellular space only. (ii) A barrier at the level of the basement membrane prevents the major fraction
of Na+ entering the lateral space from returning to the serosal bath. Thus, Na+ is secreted into the outside bath. It has to be assumed then that the Na+ permeability of the basement membrane barrier (P
BM
Na) is smaller than the Na+ permeability of the junctional membrane (P
JM
Na), i.e., P
JM
Na/P
BM
Na > 1. The secretory paracellular flow of water further requires that the Na+ reflection coefficients (σNa) of the two barriers are governed by the conditions, σBM
Na > 0, and σBM
Na > σJM
Na. (iii) Na+ channels are located in the apical membrane of the activated gland cells, so that a fraction of the Na+ outflux appearing downstream the lateral intercellular space is recirculated by the gland cells. Based on measured unidirectional
fluxes, a set of equations is developed from which we estimate the ion fluxes flowing through major pathways during stationary
secretion. It is shown that 80% of the sodium ions flowing downstream the lateral intercellular space is recycled by the gland
cells. Our calculations also indicate that under the conditions prevailing in the present experiments 1.8 ATP molecule would
be hydrolyzed for every Na+ secreted to the outside bath.
Received: 30 January 1996/Revised: 12 March 1996 相似文献
11.
R. López-Vancell G. Beaty E. Stefani E. E. Rodríguez-Boulan M. Cereijido 《The Journal of membrane biology》1984,81(3):171-180
Summary MDCK cells (epithelioid line derived from the kidney of a normal dog) form monolayers which retain the properties of transporting epithelia. In these cells viruses bud asymmetrically: influenza from the apical, and vesicular stomatitis (VSV) from the basolateral membrane (E. Rodríguez-Boulán and D. D. Sabatini,Proc. Natl. Acad. Sci. USA
75: 5071–5075, 1978; E. Rodríguez-Boulán and M. Pendergast,Cell
20: 45–54, 1980). In the present study, we analyzed whether these viruses affect specific ion-translocating mechanisms located in the plasma membrane. We studied the effect of infection on membrane and transepithelial conductance, passive and active unidirectional fluxes of Na+ and K+, intracellular potentials, cellular content of Na+ and K+, and formation of blisters which, in these preparations, are due to the vectorial transport of fluid. Two main observations are derived from these studies. First, infection with VSV caused an increase in transepithelial electrical conductance, due to the opening of tight junctions, 5 to 6 hr after the start of infection, coincident with the accumulation of envelope protein in the cell surface and with the rise in the curve of virus budding. Infection with influenza, on the other hand, increased the transepithelial conductance only late in the infection (12 to 14 hr) when virus production has already stopped. Second, viruses did affect membrane permeability. Yet, the changes observed may not be ascribed to a perturbation of the specific translocating mechanisms for Na+ and K+ which operate in the same region of the plasma membrane that the viruses use to penetrate and leave MDCK cells. The methods used in the present study are not suitable to decide whether the nonspecific changes in permeability elicited by the viruses occur over the whole cell membrane or are restricted to a given region. 相似文献
12.
The balance of K+, Na+, and Cl− fluxes across the cell membrane with the Na+/K+ pump, ion channels, and Na+K+2Cl− (NKCC) and Na+-Cl− (NC) cotransport was calculated to determine the mechanism of cell shrinkage in apoptosis. It is shown that all unidirectional
K+, Na+, and Cl− fluxes; the ion channel permeability; and the membrane potential can be found using the principle of the flux balance if
the following experimental data are known: K+, Na+, and Cl− concentrations in cell water; total Cl− flux; total K+ influx; and the ouabain-inhibited pump component of the Rb+(K+) influx. The change in different ionic pathways during apoptosis was estimated by calculations based on the data reported
in the preceded paper (Yurinskaya et al., 2010). It is found that cell shrinkage and the shift in ion balance in U937 cells
induced to apoptosis with 1 μM staurosporine occur due to the coupling of reduced pump activity with a decrease in the integral
permeability of Na+ channels, whereas K+ and Cl− channel permeability remains almost unchanged. Calculations show that only a small part of the total fluxes of K+, Na+, and Cl− account for the fluxes mediated by NKCC and NC cotransporters. Despite the importance of cotransport fluxes for maintaining
the nonequilibrium steady-state distribution of Cl−, they cannot play a significant role in apoptotic cell shrinkage because of their minority and cannot be revealed by inhibitors. 相似文献
13.
Wolfram Nagel 《生物化学与生物物理学报:生物膜》1979,552(2):346-357
The effect of Ba2+ (0.5 mM, corial side) upon the transport characteristics of the frog skin epithelium was investigated. It was observed that Ba2+ decreased the conductance of the preferably K+-permeable basolateral border to less than 30% of its control value. Furthermore, Ba2+ abolished the K+ electrode-like behaviour, existing at the basolateral membrane under conditions of zero transcellular current flow, for [K+] below 10–15 mM. Effects upon other parameters of transepithelial transport (electromotive forces and resistance of outer or basolateral border and shunt pathway, respectively) were small and might represent secondary events. It is concluded that Ba2+ inhibits passive fluxes of K+ across basolateral membranes of tight, Na+ transporting epithelia, similar to its influence upon membranes of nonpolar cells. 相似文献
14.
Summary Thein vitro effect of ethanol on membrane structure and transport properties was studied in isolated renal brush border membrane vesicles.31P-NMR studies showed a dose-dependent increase in the quantity of an isotropic, possibly inverted-micellar component of the renal brush-border membrane as a result of treatment with ethanol. Such structures have been shown to be instrumental in the translocation of material across membrane bilayers. A23Na-NMR study of Na+ exchange in artificial phosphatidylcholine liposomes indicated that ethanol (0.1%) was capable of rending the otherwise inert vesicles permeable to sodium, supporting the idea that ethanol may exert its action via a direct effect on the structure of the phospholipid bilayer. In the isolated renal brush-border membrane vesicles, like in the artificial liposomes, amiloride-insensitive pathways of Na+ transport were shown to be markedly activated by ethanol. These results were consistent with the inhibitory effect ethanol had on Na+ gradient-dependent transport systems such as the Na+ gradient-dependentd-glucose transport and Na+/H+ exchange. In conclusion, our results indicate that ethanol exerts its effect on the renal brush-border membrane by causing a structural change in the phospholipid bilayer which activates sodium intake. The inhibitory effect of ethanol on glucose uptake and Na+/H+ exchange is secondary, as a result of the dissipation of the energy-producing Na+ gradient. 相似文献
15.
Free-flow electrophoresis was used to separate microvilli from the lateral basal plasma membrane of the epithelial cells from rat small intestine. The activities of the marker enzyme for the microvillus membrane, i.e. alkaline phosphatase (EC 3.1.31), was clearly separated from the marker for the lateral-basal plasma membrane, i.e. the (Na+, K+)-ATPase (EC 3.6.1.3). A microvillus membrane fraction was obtained with a high specific activity of alkaline phosphatase (an 8-fold enrichement over the starting homogenate). The lateral-basal plasma membrane fraction contained (Na+, K+)-ATPase (5-fold over homogenate) with some alkaline phosphatase (2-fold over homogenate).Glucose transport was studied in both membrane fractions. The uptake of d-glucose was much faster than that of l-glucose in either plasma membrane, d-Glucose uptake could be accounted for completely by its transport into an osmotically active space. Interestingly, the characteristics of the glucose transport of the microvillus membrane were different from those of the lateral-basal plasma membrane. In particular: Na+ stimulated the d-glucose transport by the microvillus membrane, but not by the lateral-basal plasma membrane. In addition, the glucose transport of the microvillus membrane was much more sensitive to phlorizin inhibition than that of the lateral-basal plasma membrane.These experiments thus provide evidence not only for an asymmetrical distribution of the enzymes, but also for differences in the transport properties with respect to glucose between the two types of plasma membrane of the intestinal epithelial cell. 相似文献
16.
V. E. Yurinskaya A. V. Moshkov T. S. Goryachaya A. A. Vereninov 《Cell and Tissue Biology》2014,8(1):80-90
Lithium transport across the cell membrane is interesting in the light of general cell physiology and because of its alteration during numerous human diseases. The mechanism of Li+ transfer has been studied mainly in erythrocytes with a slow kinetics of ion exchange and therefore under the unbalanced ion distribution. Proliferating cultured cells with a rapid ion exchange have not been used practically in study of Li+ transport. In the present paper, the kinetics of Li+ uptake and exit, as well as its balanced distribution across the plasma membrane of U937 cells, were studied at minimal external Li+ concentrations and after the whole replacement of external Na+ for Li+. It is found that a balanced Li+ distribution attained at a high rate similar to that for Na+ and Cl? and that Li+/Na+ discrimination under balanced ion distribution at 1–10 mM external Li+ stays on 3 and drops to 1 following Na, K-ATPase pump blocking by ouabain. About 80% of the total Li+ flux across the plasma membrane under the balanced Li+ distribution at 5 mM external Li+ accounts for the equivalent Li+/Li+ exchange. The majority of the Li+ flux into the cell down the electrochemical gradient is a flux through channels and its small part may account for the NC and NKCC cotransport influxes. The downhill Li+ influxes are balanced by the uphill Li+ efflux involved in Li+/Na+ exchange. The Na+ flux involved in the countertransport with the Li+ accounts for about 0.5% of the total Na+ flux across the plasma membrane. The study of Li+ transport is an important approach to understanding the mechanism of the equivalent Li+/Li+/Na+/Na+ exchange, because no blockers of this mode of ion transfer are known and it cannot be revealed by electrophysiological methods. Cells cultured in the medium where Na+ is replaced for Li+ are recommended as an object for studying cells without the Na,K-ATPase pump and with very low intracellular Na+ and K+ concentration. 相似文献
17.
The effect of short circuit current on the unidirectional fluxes of ions transported across tight and leaky epithelia was investigated. It was found that short circuiting of the frog gastric mucosa (classified as a tight epithelium) caused a decrease of the passive JmsC1 and a significant increase of the net Cl? secretion. However, no significant change of H+ secretory rate was observed. On the other hand, short circuiting of the mouse intestine (a known leaky membrane) caused a simultaneous increase of both Jms and Jsm fluxes of Na+ while the net fluxes of Na+ and Cl? remained unchanged. Also, short circuiting did not change the water permeability of the mouse intestine. To explain some of these results a theoretical model is presented to demonstrate that while short circuiting can block the passive ionic movement, it will cause an increase in the energy consumption of the system and introduce certain important changes in the ionic barriers and e.m.fs. The simultaneous increase in the unidirectional fluxes of Na+ under short circuit conditions can best be explained by a decrease in the polarized nature of the transepithelial shunt, thereby increasing the diffusion coefficient of the ion(s). Such an increase is specially favorable to the Na+ rather than an anion. 相似文献
18.
To prevent sodium toxicity in plants, Na+ is excluded from the cytosol to the apoplast or the vacuole by Na+/H+ antiporters. The secondary active transport of Na+ to apoplast against its electrochemical gradient is driven by plasma membrane H+-ATPases that hydrolyze ATP and pump H+ across the plasma membrane. Current methods to determine Na+ flux rely either on the use of Na-isotopes (22Na) which require special working permission or sophisticated equipment or on indirect methods estimating changes in the H+ gradient due to H+-ATPase in the presence or absence of Na+ by pH-sensitive probes. To date, there are no methods that can directly quantify H+-ATPase-dependent Na+ transport in plasma membrane vesicles. We developed a method to measure bidirectional H+-ATPase-dependent Na+ transport in isolated membrane vesicle systems using atomic absorption spectrometry (AAS). The experiments were performed using plasma membrane-enriched vesicles isolated by aqueous two-phase partitioning from leaves of Populus tomentosa. Since most of the plasma membrane vesicles have a sealed right-side-out orientation after repeated aqueous two-phase partitioning, the ATP-binding sites of H+-ATPases are exposed towards inner side. Leaky vesicles were preloaded with Na+ sealed for the study of H+-ATPase-dependent Na+ transport. Our data implicate that Na+ movement across vesicle membranes is highly dependent on H+-ATPase activity requiring ATP and Mg2+ and displays optimum rates of 2.50 μM Na+ mg− 1 membrane protein min− 1 at pH 6.5 and 25 °C. In this study, for the first time, we establish new protocols for the preparation of sealed preloaded right-side-out vesicles for the study of H+-ATPase-dependent Na+ transport. The results demonstrate that the Na+ content of various types of plasma membrane vesicle can be directly quantified by AAS, and the results measured using AAS method were consistent with those determined by the previous established fluorescence probe method. The method is a convenient system for the study of bidirectional H+-ATPase-dependent Na+ transport with membrane vesicles. 相似文献
19.
Summary The unidirectional Na+, Cl–, and urea fluxes across isolated opercular epithelia from seawater-adaptedFundulus heteroclitus were measured under different experimental conditions. The mean Na+, Cl–, and urea permeabilities were 9.30×10–6 cm·sec–1, 1.24×10–6 cm·sec–1, and 5.05×10–7 cm·sec–1, respectively. The responses of the unidirectional Na+ fluxes and the Cl– influx (mucosa to serosa) to voltage clamping were characteristic of passively moving ions traversing only one rate-limiting barrier. The Na+ conductance varied linearly with, and comprised a mean 54% of, the total tissue ionic conductance. The Cl– influx and the urea fluxes were independent of the tissue conductance. Triaminopyrimidine (TAP) reduced the Na+ fluxes and tissue conductance over 70%, while having no effect on the Cl– influx or urea fluxes. Mucosal Na+ substitution reduced the Na+ permeability 60% and the tissue conductance 76%, but had no effect on the Cl– influx or the urea fluxes. Both the Na+ and Cl– influxes were unaffected by respective serosal substitutions, indicating the lack of any Na+/Na+ and Cl–/Cl– exchange diffusion.The results suggest that the unidirectional Na+ fluxes are simple passive fluxes proceeding extracellularly (i.e., movement through a cation-selective paracellular shunt). This pathway is dependent on mucosal (external) Na+, independent of serosal (internal) Na+, and may be distinct from the transepithelial Cl– and urea pathways. 相似文献
20.
Massaki Ando 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1985,155(3):311-317
Summary Simultaneous measurements of net ion and water fluxes were made in the stripped intestine of the seawater eel, and the relationship between Na+, K+, Cl– and water transport were examined in the presence of mucosal KCl and serosal NaCl Ringer (standard condition). When Cl– was removed from both sides of the intestine, net K+ flux from mucosa to serosa was reduced, accompanied by complete blockage of water absorption. Since it has been shown that net Cl– and water fluxes depend on K+ transport under the standard condition (Ando 1983), the interdependence of K+ and Cl– transport suggests the existence of a coupled KCl transport system, while the parallelism between the net Cl– and water fluxes suggests that water absorption is linked to the coupled KCl transport. The coupled KCl and water transport were inhibited by treatment with ouabain or with Na+-free Ringer solutions, suggesting the existence of a Na+-dependent KCl transport system and linkage of water absorption to the coupled Na+–K+–Cl– transport. Since ouabain blocked the active Na+–K+–Cl– transport almost completely, the permeability coefficients for K+ and Na+ through the paracellular shunt pathway were estimated as PK=0.076 and PNa=0.058 cm/h, and PCl was calculated as 0.005 cm/h. Although Na+-independent K+ and Cltt- fluxes were observed again in the present study, these fluxes were not inhibited by CN–, ouabain or diuretics, and evoked even after blocking the Na+–K+–Cl– transport completely with ouabain. These results indicate that the Na+-independent K+ and Cl– fluxes are distinct from the active Na+–K+–Cl– transport and are not themselves active. 相似文献