共查询到20条相似文献,搜索用时 15 毫秒
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Downstream E-box-mediated regulation of the human telomerase reverse transcriptase (hTERT) gene transcription: evidence for an endogenous mechanism of transcriptional repression 总被引:26,自引:0,他引:26 下载免费PDF全文
Horikawa I Cable PL Mazur SJ Appella E Afshari CA Barrett JC 《Molecular biology of the cell》2002,13(8):2585-2597
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Yerra Koteswara Rao Te-Yu Kao Ming-Fang Wu Jiunn-Liang Ko Yew-Min Tzeng 《Bioorganic & medicinal chemistry》2010,18(19):6987-6994
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Fujimoto K Kyo S Takakura M Kanaya T Kitagawa Y Itoh H Takahashi M Inoue M 《Nucleic acids research》2000,28(13):2557-2562
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Adenovirus-mediated suicide gene therapy using the human telomerase catalytic subunit (hTERT) gene promoter induced apoptosis of ovarian cancer cell line 总被引:20,自引:0,他引:20
Song JS Kim HP Yoon WS Lee KW Kim MH Kim KT Kim HS Kim YT 《Bioscience, biotechnology, and biochemistry》2003,67(11):2344-2350
Telomerase is a ribonucleoprotein complex the function of which is to add telomeric repeats (TTAGGG)(n) to chromosomal ends, and it is known to play an important role in cellular immortalization. Telomerase is highly active in most tumor cells, yet not in normal cells. As such, it may have possible applications in cancer gene therapy. Telomerase consists of two essential components, telomerase RNA template (hTR) and catalytic subunit (hTERT). hTERT is expressed only in cells and tissues positive for telomerase activity, i.e., tumor and fetal cells. We here tested the possibility of the utilization of the hTERT promoter in targeted cancer gene therapy. We cloned the hTERT promoter in the replace of the CMV promoter and sub-cloned HSV-TK gene to be controlled by hTERT gene promoter in adenovirus shuttle plasmid. Then we constructed recombinant adenovirus Ad-hT-TK, and infected them into normal and human gynecological cancer cell lines. Through these experiments, we identified the selective tumor specific cell death by Ad-hT-TK. Furthermore, FACS analysis and TUNEL assay suggests that the reduced viability is mediated through the induction of apoptosis, indicating that this approach may be a useful method for suppressing cancer growth in targeted cancer gene therapy. These results show that Ad-hT-TK could be used for gynecological cancer gene therapy. 相似文献
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Calcium-mediated telomerase activity in ovarian epithelial cells 总被引:4,自引:0,他引:4
Alfonso-De Matte MY Moses-Soto H Kruk PA 《Archives of biochemistry and biophysics》2002,399(2):239-244
Though the potential of telomerase as an anti-cancer target is evident, information about regulation of telomerase remains fragmentary. In the present study, we examined the role of calcium, an essential cellular signaling molecule, in the regulation of telomerase. We found that calcium induced de novo telomerase activity in telomerase-negative ovarian surface epithelial (OSE) cell lines but not in primary cultures of OSE. In addition, we showed that calcium elevated endogenous telomerase levels in a telomerase-positive ovarian cancer cell line. The use of calcium channel blockers or calcium chelators inhibited this calcium-mediated induction of telomerase activity. Furthermore, cadmium and chromium appeared to cause a moderate induction of telomerase activity while several other metal salts did not. Our data provide the first example of calcium-induced telomerase activity in human cell lines, provide a novel avenue for possible intervention of telomerase, and permit development of therapeutic agents for adjunctive chemotherapy. 相似文献