Stimulation of immunogenesis by neurotensin, pentagastrin and thymopentin and ways of its realization

In experiments on mice and in vitro the influence of neurotensin pentagastrin and thymopentin on the immune response, the phagocytosis of staphylococcus aureus by polymorphoneutrophil leucocytes and enzymatic activity of these cells by NBT-test were investigated. It was shown that neurotensin and thymopentin increase enzymatic and phagocytic function of polymorphoneutrophil leucocytes. Pentagastrin, as well as thymopentin stimulates the immune response, enzymatic but not phagocytic function of polymorphoneutrophil leucocytes. Immunostimulating effect of the studied peptides was realized by facility differentiation of mouse bone marrow cells into T-lymphocytes and by the interaction of the peptides with T-cells.     

Stimulation of antibody formation by bacterial lipopolysaccharide. The influence of repeated doses of lipopolysaccharide on antibody formation

1. (1)
   Липополисахарид стимулирует образование антител даже при 8-кратном введении в сочетании с частично очищенным дифтерийным анатоксином.     
   
   

Persistense of mono- and associated cultures of conditionally pathogenic enterobacteria

The comparative study of adhesive, hemolytic, DNA-ase, lecithinase, antilysozymic, anticomplementary activities of mono- and associated cultures of 57 Enterobacter spp., 61 Citrobacter spp. and 55 Serratia spp. strains, isolated from patients with pyoinflammatory, intestinal and urological diseases is carried out. Different variations of cocultivated bacteria including Enterobacter and Citrobacter, Enterobacter and Serratia, Citrobacter and Serratia are used. It was shown, that cocultivated Enterobacter, Citrobacter and Serratia bacteria increased the persistent properties of mixt cultures.     

attempts to influence antibody formation and resistance to infection in newborn animals by vitamin b12

Авторы попытались п овлиять иммунологи ческое с остояние мо лодых животных, кото рые обычно не образу ют антитела в ответ н а отправление антиг ена за несколько нед ель после рождения. Администрация вита мина B (in12), для молодые кр олики и щенков после рождения и беременн ых женщин на последн ем дней беременност и не влияет времени появления первых ан тител в качестве по с равнению с контроле м. Морфологический с озревание mesenchymal тканей было стимулировать, однако. Администрац ия витамина B (in12) влияет уровень пассивно пе редаются антитела в молодых животных, ес ли они присутствова ли в родном сыворот ки. Администрация вита мина B (in12), для взрослые кролики, не влияют на антитела синтез по с равнению с контроле м. Хотя администрация витамина B(in12) молодых к рыс, не ускорить разв итие антител, он выро с их устойчивость к и нфекциям производс тва ви (itBacillus anthracis). Результаты обсужда ются с аспект взаимо отношений созреван ие общих физиологич еских и метаболичес ких процессов у моло дых животных к разви тию от конкретных им мунную реакцию, Я. д. фо рмирование антител.     

Molecular mechanisms of relationships between the host organism and pathogenic enterobacteria

Modern data on the molecular mechanisms of relationships between the host organism and the pathogenic representatives of the family Enterobacteriaceae in the host-parasite system are presented. The process of cytokine and eicosanoid regulation of the immune process of the host in the norm and pathology states are analyzed. The examples of the mechanisms of immune suppression, false antigenic stimulation and the mimicry of pathogens are given.     

Attempts to influence antibody formation by tetracycline

Эффект тетрациклина на антитела образования был изучен в экспериментах на которой различные типы антигена, в адъюванта, и инактивированные приостановления Управление по внутримышечно кролики.

1. (1)
   В кроликов иммунизированных с в жидкий парафин, антитело формирование была в депрессии ежедневных внутримышечных администрации 25. тетрациклин за одиннадцать дней день иммунизации. Тетрациклин Кроме того, заметно снижение интенсивности от задержки с аллергической реакцией на администрации.     
   
   

Characterization of two different endo-alpha-N-acetylgalactosaminidases from probiotic and pathogenic enterobacteria, Bifidobacterium longum and Clostridium perfringens

Endo-alpha-N-acetylgalactosaminidase (endo-alpha-GalNAc-ase) catalyzes the hydrolysis of the O-glycosidic bond between alpha-GalNAc at the reducing end of mucin-type sugar chains and serine/threonine of proteins to release oligosaccharides. Previously, we identified the gene engBF encoding endo-alpha-GalNAc-ase from Bifidobacterium longum, which specifically released the disaccharide Gal beta 1-3GalNAc (Fujita K, Oura F, Nagamine N, Katayama T, Hiratake J, Sakata K, Kumagai H, Yamamoto K. 2005. Identification and molecular cloning of a novel glycoside hydrolase family of core 1 type O-glycan-specific endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum. J Biol Chem. 280:37415-37422). Here we cloned a similar gene named engCP from Clostridium perfringens, a pathogenic enterobacterium, and characterized the gene product EngCP. Detailed analyses on substrate specificities of EngCP and EngBF using a series of p-nitrophenyl-alpha-glycosides chemically synthesized by the di-tert-butylsilylene-directed method revealed that both enzymes released Hex/HexNAc beta 1-3GalNAc (Hex = Gal or Glc). EngCP could also release the core 2 trisaccharide Gal beta 1-3(GlcNAc beta 1-6)GalNAc, core 8 disaccharide Gal alpha 1-3GalNAc, and monosaccharide GalNAc. Our results suggest that EngCP possesses broader substrate specificity than EngBF. Actions of the two enzymes on native glycoproteins and cell surface glycoproteins were also investigated.