Humic substances change during the co-composting process of municipal solid wastes and sewage sludge

The change of the degree of stability of compost during the composting process was a kind of guideline for our study. This stability was estimated by monitoring the chemical fractionation (extraction of humic and fulvic acids, and humin) during two cycles of composting. Change of humin (H), humic-like acid carbon (CHA) and fulvic-like acid carbon (CFA) fractions during the composting process of municipal solid wastes were investigated using two windrows W1 (100% of municipal solid wastes) and W2 (60% of municipal solid wastes and 40% of dried sewage sludge). Humin and fulvic acid fractions in the two windrows decreased since the start of composting process and tend to stabilize. At the end of composting process, humic acid fraction is more important in the windrow without sludge (W1) than the one with sludge (W2). The humification indexes used in this study showed that the humic-like acid carbon fraction production takes place largely during the phase of temperature increase (thermophilic phase), and it appeared very active in the windrow W2. At the end of composting process, the E₄/E₆ ratio value indicated that the compost of W1 is more mature than the compost of W2. The humification ratio (HR) allowed a correct estimation of compost organic matter stabilization level.     

Characterization of fresh yard trimmings for agricultural use

Direct application of yard trimmings to agricultural land can benefit soils and crop production, while providing an outlet for handling high volumes of materials at compost facilities. Variability in the composition of yard trimmings can make it difficult to determine appropriate application rates. Our objective was to characterize the chemical composition and variability of yard trimmings generated throughout the spring and summer season at facilities in the Puget Sound region of Washington State. Yard trimmings were sampled from four composting facilities on five dates between April and August 1999. One material contained mostly grass clippings and had higher mean total N (3.2%) than mixed grass and woody materials (1.5-2%). Mean C:N was lower in the grass-rich material (12:1 vs. 15 to 21:1), while mean ammonium concentrations were similar (0.18-0.28%). Variation among facilities was greater than variation over time. The amount of variation observed with other nutrients, pH, EC, or trace elements would not affect use of the yard trimmings in agriculture. Our results suggest that it is possible to characterize yard trimmings adequately for agricultural use.     

Bio-degradation of olive mill wastewater sludge by its co-composting with agricultural wastes

The use of maize straw (MS) or cotton waste (CW) as bulking agents in the composting of olive mill wastewater (OMW) sludge was compared by studying the organic matter (OM) mineralisation and humification processes during composting and the characteristics of the end products. Both composts were prepared in a pilot-plant using the Rutgers static-pile system. The use of CW instead of MS to compost OMW sludge extended both the thermophilic and bio-oxidative phases of the process, with higher degradation of polymers (mainly lignin and cellulose), a greater formation of nitrates, higher total nitrogen losses and a lower biological nitrogen fixation. The CW produced a compost with a more stabilised OM and more highly polymerised humic-like substances. In the pile with CW and OMW sludge, OM losses followed a first-order kinetic equation, due to OM degradation being greater at the beginning of the composting and remaining almost constant until the end of the process. However, in the pile with MS and OMW sludge this parameter followed a zero-order kinetic equation, since OM degraded throughout the process. The germination index indicated the reduction of phytotoxicity during composting.     

Heat inactivation of Salmonella spp. in fresh poultry compost by simulating early phase of composting process

Aims: The purpose of this study was to determine the effect of moisture on thermal inactivation of Salmonella spp. in poultry litter under optimal composting conditions. Methods and Results: Thermal inactivation of Salmonella was studied in fresh poultry compost by simulating early phase of composting process. A mixture of three Salmonella serotypes grown in Tryptic soy broth with rifampin (TSB‐R) was inoculated in fresh compost with 40 or 50% moisture at a final concentration of c. 7 log CFU g^?1. The inoculated compost was kept in an environmental chamber which was programmed to rise from room temperature to target composting temperatures in 2 days. In poultry compost with optimal moisture content (50%), Salmonella spp. survived for 96, 72 and 24 h at 50, 55 and 60°C, respectively, as compared with 264, 144 and 72 h at 50, 55 and 60°C, respectively, in compost with suboptimal moisture (40%). Pathogen decline was faster during the come‐up time owing to higher ammonia volatilization. Conclusions: Our results demonstrated that Salmonella spp. survived longer in fresh poultry compost with suboptimal moisture of 40% than in compost with optimal moisture of 50% during thermophilic composting. High nitrogen content of the poultry compost is an additional factor contributing to Salmonella inactivation through ammonia volatilization during thermal exposure. Significance and Impact of the Study: This research validated the effectiveness of the current composting guidelines on Salmonella inactivation in fresh poultry compost. Both initial moisture level and ammonia volatilization are important factors affecting microbiological safety and quality of compost product.     

Organic matter transformations and kinetics during sewage sludge composting in a two-stage system

The use of different proportions of rape straw and grass as amendments in the composting of dewatered sewage sludge from a municipal wastewater treatment plant was tested in a two-stage system (first stage, an aerated bioreactor and second stage, a periodically turned windrow). The composition of feedstock affected the temperature and organic matter degradation in the bioreactor and the formation of humic substances, especially humic acids (HA), during compost maturation in the windrow. The total HA content (the sum of labile and stable HA) increased according to first-order kinetics, whereas labile HA content was constant and did not exceed 12% of total HA. Δlog K of 1.0–1.1 indicated that HA was of R-type, indicating a low degree of humification. Temperature during composting was the main factor affecting polymerization of fulvic acids to HA and confirmed the value of the degree of polymerization, which increased only when thermophilic conditions were obtained.     

Ash in composting of source-separated catering waste

Our earlier experiments in small composters (220 l) indicated the favourable effect of ash from co-incineration of sorted dry waste on the composting of catering waste. The aim of this new study was to clarify further, at a scale of 10 m3, the feasibility of using similar ash as an additive in composting. Source-separated catering waste was mixed with bulking agent (peat and wood chips) and fuel ash from a small (4 MW) district heating power plant. Three compost mixes (CM) were obtained: CM I with 0%, CM II with 10% and CM III with 20 wt.% of fuel ash. These three different mixes were composted in a 10-m3 drum composter as three parallel experiments for 2 weeks each, from January to April 2000. After drum composting, masses were placed according to mixing proportions in separate curing piles. The catering waste fed to the drum was cold, sometimes icy. Even then the temperature rapidly increased to over 50 degrees C. In CM III, the temperature rose as high as 80 degrees C, and after the first week of composting the temperature was about 20 degrees C higher in the CMs II and III than in the CM I. It also improved the oxygen concentrations at the feeding end of the drum and obviously prevented the formation of H2S. No odour problems arose during the composting. Addition of ash increased the heavy metal contents of the composting masses, but the compost was suitable for cultivation or green area construction. Ash clearly decreased the loss of total nitrogen in a time span of 2 years. The lower amounts of nitrogen mean that the amounts applied per hectare can be greater than for normal composts. Measured by mineralization, the breaking down of the organic matter was more rapid in the CM III than in the CM I. Humic acid increased steadily during first 12 months composting, from the initial 39 mg/g organic matter to 115 and 137 mg/g in CMs II and III. Measured by temperature, mineralization and humification the addition of ash appeared to boost the composting. Ash had also other beneficial effects on composting it improved the availability of oxygen in compost mass during the drum composting phase and reduced the formation of odorous gases, especially H2S.     

Transformation of rock phosphate during composting and the effect of humic acid

Summary Phosphorus from Mussoorie rock phosphate (MRP) was solubilized and transformed into available forms when MRP was incorporated during composting of organic wastes. Clusterbean and redgram utilized phosphorus efficiently from the phosphorus enriched compost containing 3.1% P when added in the soil of pH 7.6 to 7.8. The solubilization of phosphorus during composting has been attributed to the formation of humic substances.     

Characterization of dairy cattle manure/wallboard paper compost mixture

The aim of this research was to evaluate the use of manufacturing wallboard paper scraps as an alternative bulking agent for dairy cattle manure composting. The characteristics of the composting process were studied based on the changes in physico-chemical parameters and final compost quality. Composting of dairy cattle manure with wallboard paper was performed in a 481-L cylindrical reactor with vacuum-type aeration. Rapid degradation of organic matter was observed during the thermophilic stage of composting due to high microbial activity. High temperature and alkaline pH conditions promoted intense ammonia emission during the early stage of composting. The number of mesophilic and thermophilic microorganisms were found to be affected by changes in temperature at different composting stages. The total nitrogen (N), phosphorus (P), potassium (K), and sodium (Na) concentrations of the mixture did not change significantly after 28days of composting. However, the presence of gypsum in the paper scraps increased the calcium content of the final compost. The wallboard paper had no phyto-inhibitory effects as shown by high germination index of final compost (GI=99%).     

Influence of green waste, biowaste and paper-cardboard initial ratios on organic matter transformations during composting

The influence of green waste, biowaste and paper-cardboard proportions in initial mixtures on organic matter (OM) evolution during composting in pilot-scale reactors was studied using respirometric procedure, humic substance extraction, crude fiber analysis and Fourier transform infrared spectroscopy. The stabilisation of OM during composting resulted from the degradation of easily biodegradable organic fraction as cellulose and hemicellulose, the relative increase of resistant compounds as lignin, the microbial synthesis of resistant biomolecules, and from humification processes. Little stabilisation of green waste OM during composting was observed, in relation with their large lignin content. With moderate contents of paper-cardboard in initial mixtures (20-40%), cellulose proportion remained favorable to fast OM stabilisation. Larger proportions of paper-cardboard (more than 50%) affected OM stabilisation, probably due to a lack of nitrogen. The influence of biowastes only appeared at the very beginning of composting, because of their large proportions of easily biodegradable OM.     

Characterization of natural organic matter (NOM) derived from sewage sludge compost. Part 1: chemical and spectroscopic properties

In this study changes in the properties of natural organic matter (NOM) were studied during composting of sewage sludge in a laboratory experiment using the pile method. Typical physicochemical parameters were measured during 53 days of composting including humic fractions. The effects of humification on the molecular properties of humic acids (HA) were investigated by 13C CP/MAS NMR spectroscopy. On the basis of chemical analyses, 53 days of composting sewage sludge with structural material can be divided into three phases: (i) domination of rapid decomposition of non-humic, easily biodegradable organic matter (two to three weeks), (ii) domination of organic matter humification and formation of polycondensed, humic-like substances (the next two weeks), (iii) stabilization of transformed organic material and weak microbial activity. Spectroscopic characterization (13C NMR) of compost humic acids reveals changes in their structures during maturation. The changes are highly correlated with the processes taking place in bulk compost.     

Methods to improve the composting process of the solid fraction of dairy cattle slurry

Cattle slurry solid fraction (SF) with different dry matter (DM) contents was collected from two dairy farms and composted in static and turned piles, with different sizes and cover types, to investigate the effects of pile conditions on the physical and chemical changes in SF during composting and to identify approaches to improve final compost quality. Thermophilic temperatures were attained soon after separation of SF, but the temperature of piles covered with polyethylene did not increase above 60 degrees C. The rate of organic matter (OM) mineralisation increased for turned piles in comparison to static piles, but the maximum amount of mineralisable OM (630-675gkg(-1)) was similar for all pile treatments. The C/N ratio declined from over 36 to a value of 14 towards the end of composting, indicating an advanced degree of OM stabilisation. Mature compost was obtained from raw SF feedstock as indicated by the low compost temperature, low C/N ratio, and low content of NH(4)(+) combined with increased concentrations of NO(3)(-). The efficiency of the composting process was improved and NH(3)-N losses were minimized by increasing DM content of the SF, reducing the frequency of pile turning and managing compost piles without an impermeable cover.     

Composting of fish offal and biosolids in northwestern Patagonia.

Composting of fish processing wastes and biosolids with wood by-products and yard trimmings was conducted during the summer of 1996 and winter of 1997 in NW Patagonia using: (i) static piles for fish offal and (ii) turning piles for biosolids. Fish offal was mixed with sawdust + wood shavings (FOC) at 3:1 ratio by weight and biosolids with wood shavings (BCw) and yard trimmings (BCt) at 1:1 ratio by volume. Samples were taken at six dates during the composting period and analyzed to determine the factors that predict compost maturity. Composting of biosolids was affected by the type of bulking agent during winter. Thermophilic temperatures > or = 55 degrees C were sustained long enough to satisfy the USEPA requirements for processes to further reduce pathogens (PFRP) in FOC and BCt, and for processes to significantly reduce pathogens (PSRP) in summer BCw, while in winter BCw temperatures were lower than those recommended for effective pathogen reduction. However, coliform fecal content in all BC treatments was less than 10 most probable number (MPN) g(-1) dry sample at the end of the process. The ratio of water soluble carbon (WSC) to total nitrogen (TN) appeared to be a more adequate index to predict compost maturity than the ratio of total organic carbon to nitrogen.     

Effect of the raw materials and mixing ratio of composted wastes on the dynamic of organic matter stabilization and nitrogen availability in composts of Sub-Saharan Africa

The effect of raw materials and their proportions in initial mixtures on organic matter (OM) stabilization and nitrogen (N) availability during pit composting in Sub-Saharan Africa was assessed using biochemical fractionation and laboratory incubations to characterize composts sampled throughout the composting process. Stabilization of OM occurred more rapidly in mixtures with slaughter-house wastes, it was progressive in mixture with household refuses while tree leaves compost remained unstable. Carbon mineralization from compost samples was positively correlated to water soluble and hemicellulose-like organic fractions. Mixtures containing large proportions of household refuses reached the highest stability and total N but available N remained weak. Slaughter-house wastes in the initial mixtures made possible to reach good OM stabilization and the largest N availability. The nature of initial mixing influenced composting parameters, OM stabilization and N availability. It is suggested mixing household refuses and slaughter-house wastes with tree leaves to reach better amending and fertilizer qualities of composts.     

Dynamics of pruning waste and spent horse litter co-composting as determined by chemical parameters

Co-composting of pruning waste and horse manure was monitored by different parameters. A windrow composting pile, having the dimensions 2.5m (height) x 30m (length) was established. The maturation of pruning waste and horse manure compost was accompanied by a decline in NH(4)(+)-N concentration, water soluble C and an increase in NO(3)(-)-N content. Organic matter (OM) content during composting followed a first-order kinetic equation. This result was in agreement with the microbiological activity measured by the CO(2) respiration during the process. The correlation at a high level of probability found between the OM loss and CO(2) evolution showed that both parameters could be used to indicate the degree of OM degradation that is the maturity and stability phases of the compost studied. Humification parameters data from the organic matter fractionation did not show a clear tendency during the composting time, suggesting that these parameters are not suitable for evaluating the dynamics of the process.     

Novel static composting method for bioremediation of olive mill waste

This paper presents results obtained on the evaluation of static composting process aimed at bioremediation of the hazardous solid olive mill waste (OMW). The static composting process carried out in gas-permeable polyethylene bags followed the fluctuating temperature and oxygen profiles similar to those seen in aerated composting systems. Static composting resulted in apparent increases and decreases in values for total nitrogen and C:N ratios respectively during the process. The amount of nitrogen (>3%) in the composting end product was in agreement with the Italian legislation (Decreto Legislativo 29 aprile 2010, n. 75) specification for nitrogen fertilizer. A gradual decrease in polyphenols during the storage of compost resulted in a non-phytotoxic composted organic matter high in humic substances. Different respirometric tests also stated high biological stability of the end compost product.     

Changes in physical,chemical and microbial parameters during the composting of municipal sewage sludge

Changes in physical, chemical and microbial parameters were investigated during the composting of municipal sewage sludge. Raw sewage sludge (30% dry matter) was mixed with compost from sewage sludge (85% dry matter) in 3:1 ratio (v/v). The mixture was divided into 4 windrows which were composted under the same conditions except the turning factor. The turning was every 7, 10, 15 days and according to the temperature which must be (55–65°C) for windrow 1 (W1), windrow 2 (W2), windrow 3 (W3) and windrow 4 (W4), respectively. Water was added to adjust the moisture content (40–60%). The composting process consisted of 2 periods; fermentation (12 weeks) and maturation (4 weeks). The results showed that the temperature reached the maximum after 12 weeks for W1 and 11 weeks for W2, W3 and W4 and then decreased. The final compost was nearly odourless and black, especially in case of W4. The general trend indicates a decrease in organic matter, organic carbon and nitrogen (N), whereas ash, potassium (K) and phosphorus (P) increased and consequently C/K and C/P ratios decreased. There was a slight increase in C/N ratio. The pH increased and then decreased to near neutrality at the end. The mesophilic bacteria increased during the fermentation period and decreased after that, whereas the thermophilic ones increased with increasing of temperature, decreased after 2 weeks and increased again during the fermentation period and then decreased. The mesophilic and thermophilic fungi were present during the first week and disappeared after that. The final compost was pathogens-free as indicated by the counts of coliforms and Salmonella.     

Temporal change of composition and potential activity of the thermophilic archaeal community during the composting of organic material

To date, composting has been regarded as an aerobic process but it has been shown that composting piles are often sources of atmospheric methane. In order to gain a more comprehensive view on the diversity of methanogenic Archaea in compost, gas chromatographical methods and molecular cloning were used to study relationships of thermophilic archaeal communities and changes in methane production potential during compost maturation. According to the thermophilic methane production potential, wide differences could be detected between differently aged compost materials. In material derived from 3- and 4-week-old piles, low and no thermophilic methane production potential, respectively, was observed at 50 degrees C. Material from a 6-week-old pile showed the maximum methane production. With compost maturation, the production slowly decreased again with 6 weeks, 8 weeks, and mature compost showing an optimum methane production potential at 60 degrees C. At 70 degrees C, only 6-week-old material showed a comparable high production of methane. The 16S rRNA-based phylogenetic surveys revealed an increase of archaeal diversity with compost maturation. In the 6-week-old material, 86% of the sequences in the archaeal 16S rRNA library had the highest sequence similarities to Methanothermobacter spp. and the remaining 14% of the clones were related to Methanosarcina thermophila. Quantification of methanogens in 6-week-old material, on the basis of the methane production rate, resulted in values of about 2x10(7) cells per gram fresh weight. In 8-week-old and mature compost material, the proportion of sequences similar to Methanothermobacter spp. decreased to 34% and 0%, respectively. The mature compost material showed the highest variation in identified sequences, although 33% could be assigned to as yet uncultured Archaea (e.g. Rice cluster I, III, and IV). Our results indicate that compost harbours a diverse community of thermophilic methanogens, with changing composition during the maturation process, presumably due to altered pile conditions. Likewise, compost may act as a potential carrier for thermophilic methanogens in temperate soils because it is widely used as a soil amendment.     

Mineralization of 2,4-dichlorophenoxyacetic acid (2,4-D) in soil inoculated with Pseudomonas cepacia DBO1(pRO101), Alcaligenes eutrophus AEO106(pRO101) and Alcaligenes eutrophus JMP134(pJP4): effects of inoculation level and substrate concentration

Mineralization of 2,4-dichlorophenoxyacetic acid (2,4-D) by two Alcaligenes eutrophus strains and one Pseudomonas cepacia strain containing the 2,4-D degrading plasmids pJP4 or pRO101 (=pJP4::Tn1721) was tested in 50 g (wet wt) samples of non-sterile soil. Mineralization was measured as ¹⁴C-CO₂evolved during degradation of uniformly-ring-labelled ¹⁴C-2,4-D. When the strains were inoculated to a level of approximately 10⁸ CFU/g soil, between 20 and 45% of the added 2,4-D (0.05 ppm, 10 ppm or 500 ppm) was mineralized within 72 h. Mineralization of 0.05 ppm and 10 ppm, 2,4-D by the two A. eutrophus strains was identical and rapid whereas mineralization by P. cepacia DBO1(pRO101) occurred more slowly. In contrast, mineralization of 500 ppm 2,4-D by the two A. eutrophus strains was very slow whereas mineralization by P. cepacia DBO1 was more rapid. Comparison of 2,4-D mineralization at different levels of inoculation with P. cepacia DBO1(pRO101) (6×10⁴, 6×10⁶ and 1×10⁸ CFU/g soil) revealed that the maximum mineralization rate was reached earlier with the high inoculation levels than with the low level. The kinetics of mineralization were evaluated by nonlinear regression analysis using five different models. The linear or the logarithmic form of a three-half-order model were found to be the most appropriate models for describing 2,4-D mineralization in soil. In the cases in which the logarithmic form of the three-half-order model was the most appropriate model we found, in accordance with the assumptions of the model, a significant growth of the inoculated strains.Abbreviations 2,4-D 2,4-dichlorophenoxyacetic acid - CFU colony forming units - PTYG peptone, tryptone, yeast & glucose - DPM disintegrations per minute     

Isolation of thermophilic ammonium-tolerant bacterium and its application to reduce ammonia emission during composting of animal wastes

A thermophilic bacterium, strain TAT105, was isolated from compost made of animal wastes. TAT105 had high tolerance to ammonium nitrogen up to 1200 mM, and highly assimilated nitrogen during the growth on swine feces. The strain was classified into Bacillus, close to Bacillus pallidus. To evaluate the effect of adding TAT105 to ammonia (NH3) emission during the composting process of animal wastes, laboratory scale composting was done. NH3 emission tended to be lower and nitrogen loss was smaller in the TAT105-added material than in the control material to which TAT105 was not added. Thermophilic ammonium-tolerant bacteria in the TAT105-added material increased to about 8x10(9) CFU/g of dry matter on the average during the tests, and most of them were judged to be TAT105 from morphological colony discrimination. These results suggested the possibility of reducing NH3 emission from composting of animal wastes by adding TAT105.     

UV spectroscopy: a tool for monitoring humification and for proposing an index of the maturity of compost

Composting organic matter is an interesting way to valorize waste. Compost is a product obtained after a humification process. The humification of organic matter during composting was studied by the quantification and monitoring of the evolution of humic substances. The final objective was to be able to evaluate the state of humification based on a spectrum of total humic substances using the method of UV spectral deconvolution. This study presented a new index obtained by UV spectroscopy using the deconvolution of an unknown spectrum of compost with 3 reference spectra. This index was compared to the maturity indices usually used, such as total extraction of humic substances (rate of extraction), IP (index of polymerization) or the humic acid to fulvic acid ratio. This new parameter, which was easy and quick to determine, gave precise information about the quality of the compost. It made it possible to disregard the values of aberrant concentrations caused by the classical protocol of extraction. Compared to the humic acid to fulvic acid ratio, the new index obtained by UV spectral deconvolution showed more representative results: the humic acid to fulvic acid ratio for an apparently non-mature compost was abnormally high, suggesting that the compost was mature, whereas the UV index proposed showed that the compost was really young and not yet humified.