共查询到20条相似文献,搜索用时 15 毫秒
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Zhao-Jun Wei Miao Yu Shun-Ming Tang Yong-Zhu Yi Gui-Yun Hong Shao-Tong Jiang 《Molecular biology reports》2011,38(2):1121-1127
Prothoracicotropic hormone (PTTH) is one of key players in regulation of insect growth, molting, metamorphosis, diapause,
and is expressed specifically in the two pairs of lateral PTTH-producing neurosecretory cells in the brain. Analysis of cis-regulatory elements of the PTTH promoter might elucidate the regulatory mechanism controlling PTTH expression. In this study,
the PTTH gene promoter of Bombyx mori (Bom-PTTH) was cloned and sequenced. The cis-regulatory elements in Bom-PTTH gene promoter were predicted using Matinspector software, including myocyte-specific enhancer
factor 2, pre-B-cell leukemia homeobox 1, TATA box, etc. Transient transfection assays using a series of fragments linked
to the luciferase reporter gene indicated that the fragment spanning −110 to +33 bp of the Bom-PTTH promoter showed high ability
to support reporter gene expression, but the region of +34 to +192 bp and −512 to −111 bp repressed the promoter activity
in the BmN and Bm5 cell lines. Electrophoretic mobility shift assays demonstrated that the nuclear protein could specifically
bind to the region spanning −124 to −6 bp of the Bom-PTTH promoter. Furthermore, we observed that the nuclear protein could
specifically bind to the −59 to −30 bp region of the Bom-PTTH promoter. A classical TATA box, TATATAA, localized at positions
−47 to −41 bp, which is a potential site for interaction with TATA box binding protein (TBP). Mutation of this TATA box resulted
in no distinct binding band. Taken together, TATA box was involved in regulation of PTTH gene expression in B. mori. 相似文献
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SBgLR (Solanum tuberosum genomic lysine-rich) is a pollen-specific gene cloned from potato (Solanum tuberosum L.). The region from −269 to −9 (The A of translation start site “ATG” as +1) of the SBgLR promoter was identified as critical for gene specific expression in pollen grains. Sequence analysis indicates a palindromic
sequence “TTTCTATTATAATAGAAA” in the −227 to −209 region, in which two pollen-specific motifs TTTCT and AGAAA surround a unique
putative TATA box. Moreover, nine putative pollen-specific motifs are located in the region between the TATA box and ATG.
We placed the −227 to −9 region (reserving the palindrome) and the −222 to −9 region (breaking the palindrome) downstream
of the CaMV35S enhancer, respectively, to construct two fusion promoters. Histochemical assays in transgenic plants demonstrated that the
region from −222 to −9 is necessary and sufficient for pollen-specific expression of the uidA gene. However, the region of −227 to −9 is incapable of driving GUS expression in pollen grains and parts of vegetative tissues.
A series of 5′ deletions from −269 to −9 of SBgLR promoter were constructed. A transient expression assay indicated that the region from the −227 to −9 suppressed gfp gene expression in pollen, and a positive regulatory element was present in the region of −253 to −227. The function of the
palindromic sequence as a repressor inhibiting gene expression in pollen was further confirmed by the mutated promoter, breaking
the palindrome by substituting its 3′-flanking five base pairs, which resumes the reporter gene expression in mature pollen. 相似文献
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K Nawa T Nakamura A Kumatori C Noda A Ichihara 《The Journal of biological chemistry》1986,261(36):16883-16888
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Xin-Wen Hu Si-Xin Liu Jian-Chun Guo Ji-Tao Li Rui-Jun Duan Shao-Ping Fu 《Functional & integrative genomics》2009,9(3):351-361
Mabinlin II is one of the major sweet proteins stored in the seeds of Capparis masaikai Lévl. Its promoter region (779 bp) located 5′ upstream of the mabinlin II gene has been isolated and named as MBL-779 (GenBank
accession number, EU014073). This promoter contains two typical TATA box regions and a series of motifs related to seed-specific
promoters, such as ACGT motifs, RY motif, napin motif, and G box. The MBL-779 promoter drove GUS gene to transiently express in the embryos of bean, maize, and rice seeds or to constantly express in the embryos and anthers
of the transgenic Arabidopsis. The MBL-779 promoter regulated gene expression from approximately the 12th day and peaked on approximately the 16th day
after flowering in Arabidopsis. The −300-bp promoter region is a minimal sequence required to functionally regulate gene expression. The CAATs at −325 to
−322 bp and −419 to −416 bp and the region at −485 to −770 bp play a role in the quantitative regulation of gene expression.
The RY motif, CATGAC, at −117 to −112 bp and the ACGT within the G box (CACGTG) at −126 to −123 bp positively regulate gene
expression.
X.-W. Hu and S.-X. Liu have the same contribution as first author. 相似文献
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Knutson A Castaño E Oelgeschläger T Roeder RG Westin G 《The Journal of biological chemistry》2000,275(19):14190-14197