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Early nitrogen‐deprivation responses in Arabidopsis roots reveal distinct differences on transcriptome and (phospho‐) proteome levels between nitrate and ammonium nutrition
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Jochen Menz Zhi Li Waltraud X. Schulze Uwe Ludewig 《The Plant journal : for cell and molecular biology》2016,88(5):717-734
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Devang Mehta Mina Ghahremani Maria Pérez-Fernández Maryalle Tan Pascal Schläpfer William C. Plaxton R. Glen Uhrig 《The Plant journal : for cell and molecular biology》2021,105(4):924-941
Phosphorus absorbed in the form of phosphate (H2PO4−) is an essential but limiting macronutrient for plant growth and agricultural productivity. A comprehensive understanding of how plants respond to phosphate starvation is essential for the development of more phosphate-efficient crops. Here we employed label-free proteomics and phosphoproteomics to quantify protein-level responses to 48 h of phosphate versus phosphite (H2PO3−) resupply to phosphate-deprived Arabidopsis thaliana suspension cells. Phosphite is similarly sensed, taken up and transported by plant cells as phosphate, but cannot be metabolized or used as a nutrient. Phosphite is thus a useful tool for differentiating between non-specific processes related to phosphate sensing and transport and specific responses to phosphorus nutrition. We found that responses to phosphate versus phosphite resupply occurred mainly at the level of protein phosphorylation, complemented by limited changes in protein abundance, primarily in protein translation, phosphate transport and scavenging, and central metabolism proteins. Altered phosphorylation of proteins involved in core processes such as translation, RNA splicing and kinase signaling was especially important. We also found differential phosphorylation in response to phosphate and phosphite in 69 proteins, including splicing factors, translation factors, the PHT1;4 phosphate transporter and the HAT1 histone acetyltransferase – potential phospho-switches signaling changes in phosphorus nutrition. Our study illuminates several new aspects of the phosphate starvation response and identifies important targets for further investigation and potential crop improvement. 相似文献
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Regulation of nitrate assimilation in cyanobacteria 总被引:1,自引:0,他引:1
Ohashi Y Shi W Takatani N Aichi M Maeda S Watanabe S Yoshikawa H Omata T 《Journal of experimental botany》2011,62(4):1411-1424
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L. J. Yu J. R. Wu Z. Z. Zheng C. C. Lin X. B. Zhan 《Applied Biochemistry and Microbiology》2011,47(5):487-493
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H M Lee E Flores K Forchhammer A Herrero N Tandeau De Marsac 《European journal of biochemistry》2000,267(2):591-600
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Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen 总被引:22,自引:0,他引:22
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Scheible WR Morcuende R Czechowski T Fritz C Osuna D Palacios-Rojas N Schindelasch D Thimm O Udvardi MK Stitt M 《Plant physiology》2004,136(1):2483-2499
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Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon-deprived Arabidopsis seedlings 总被引:9,自引:0,他引:9
Osuna D Usadel B Morcuende R Gibon Y Bläsing OE Höhne M Günter M Kamlage B Trethewey R Scheible WR Stitt M 《The Plant journal : for cell and molecular biology》2007,49(3):463-491
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A combined proteomic and isotope tracer approach was used to investigate the impact of supplying N as glycine to roots of Lolium perenne. Initially, ammonium nitrate was supplied to all plants, after which half received glycine as their sole N source, while the remainder continued to receive ammonium nitrate. Plants supplied with glycine acquired less N than those receiving the mineral source, resulting in reduced root nitrate concentrations. The amino acid complement of roots was also strongly affected by the form of N supplied, and 15N labelling indicated that the biochemical fate of acquired N in roots was dependent on the form of N available for uptake. Proteomic analysis of Lolium roots indicated that 6% of 627 root proteins resolved on 2D gels changed in abundance in response to the form of N applied. Multivariate analysis of protein abundance clearly discriminated the proteomes of L. perenne roots as a function of treatment applied. Seven affected proteins were identified (mostly by protein homology with sequenced species), including methionine adenosyltransferase, an enzyme involved in glycine metabolism. Although some changes in root amino acid and protein complements were due to responses to reduced N supply, both the distinct fate of 15N tracers and the abundances of identified proteins could be attributed specifically to the form of N available to roots. The results demonstrate the potential of targeted proteomic approaches to identify functioning of plants where more traditional methods cannot resolve multiple, co-incident biological interactions and element fluxes. 相似文献
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Immunological approach to the regulation of nitrate reductase in Monoraphidium braunii 总被引:3,自引:0,他引:3
The effects of different culture conditions on nitrate reductase activity and nitrate reductase protein from Monoraphidium braunii have been studied, using two different immunological techniques, rocket immunoelectrophoresis and an enzyme-linked immunosorbent assay, to determine nitrate reductase protein. The nitrogen sources ammonium and glutamine repressed nitrate reductase synthesis, while nitrite, alanine, and glutamate acted as derepressors. There was a four- to eightfold increase of nitrate reductase activity and a twofold increase of nitrate reductase protein under conditions of nitrogen starvation versus growth on nitrate. Nitrate reductase synthesis was repressed in darkness. However, when Monoraphidium was grown under heterotrophic conditions with glucose as the carbon and energy source, the synthesis of nitrate reductase was maintained. With ammonium or darkness, changes in nitrate reductase activity correlated fairly well with changes in nitrate reductase protein, indicating that in both cases loss of activity was due to repression and not to inactivation of the enzyme. Experiments using methionine sulfoximine, to inhibit ammonium assimilation, showed that ammonium per se and not a product of its metabolism was the corepressor of the enzyme. The appearance of nitrate reductase activity after transferring the cells to induction media was prevented by cycloheximide and by 6-methylpurine, although in this latter case the effect was observed only in cells preincubated with the inhibitor for 1 h before the induction period. 相似文献
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Integrated analysis of rice transcriptomic and metabolomic responses to elevated night temperatures identifies sensitivity‐ and tolerance‐related profiles
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Ulrike Glaubitz Xia Li Sandra Schaedel Alexander Erban Ronan Sulpice Joachim Kopka Dirk K. Hincha Ellen Zuther 《Plant, cell & environment》2017,40(1):121-137