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1.
Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation 总被引:5,自引:0,他引:5
Barbara Demmig-Adams William W. Adams III David H. Barker Barry A. Logan David R. Bowling Amy S. Verhoeven 《Physiologia plantarum》1996,98(2):253-264
In the present study we explored the possibility of assessing the allocation of photons absorbed by photosystem II (PSII) antennae to thermal energy dissipation and photosynthetic electron transport in leaves of several plant species under field conditions. Changes in chlorophyll fluorescence parameters were determined in situ over the course of an entire day in the field in sun-exposed leaves of two species with different maximal rates of photosynthesis, Helianthus annuus (sunflower) and Vinca major. Leaves of Vinca minor (periwinkle) growing in a deeply shaded location were also monitored. We propose using diurnal changes in the efficiency of open PSII centers (F′v/F′m) in these sun and shade leaves to (a) assess diurnal changes in the allocation of absorbed light to photochemistry and thermal energy dissipation and, furthermore, (b) make an estimate of changes in the rate of thermal energy dissipation, an analogous expression to the rate of photochemistry. The fraction of light absorbed in PSII antennae that is dissipated thermally (D) is proposed to be estimated from D = 1-F′v/F′m, in analogy to the widely used estimation of the fraction of light absorbed in PSII antennae (P) that is utilized in PSII photochemistry from P = F′v/F′m× qP (where qP is the coefficient for photochemical quenching; Genty, B., Briantais, J.-M. & Baker, N. R. 1989. Biochim. Biophys. Acta 990: 87-92). The rate of thermal dissipation is consequently given by D × PFD (photon flux density), again in analogy to the rate of photochemistry P × PFD, both assuming a matching behavior of photosystems I and II. Characterization of energy dissipation from the efficiency of open PSII centers allows an assessment from a single set of measurements at any time of day; this is particularly useful under field conditions where the fully relaxed reference values of variable or maximal fluorescence needed for the computation of nonphotochemical quenching may not be available. The usefulness of the assessment described above is compared with other currently used parameters to quantify nonphotochemical and photochemical chlorophyll fluorescence quenching. 相似文献
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Profiles of chlorophyll fluorescence were measured in spinach leaves irradiated with monochromatic light. The characteristics of the profiles within the mesophyll were determined by the optical properties of the leaf tissue and the spectral quality of the actinic light. When leaves were infiltrated with 10?4M DCMU [3‐(3,4‐dichlorophenyl)‐1, 1‐dimethyl‐urea] or water, treatments that minimized light scattering, irradiation with 2000 μmol m?2 s?1 green light produced broad Gaussian‐shaped fluorescence profiles that spanned most of the mesophyll. Profiles for chlorophyll fluorescence in the red (680 ± 16 nm) and far red (λ > 710 nm) were similar except that there was elevated red fluorescence near the adaxial leaf surface relative to far red fluorescence. Fluorescence profiles were narrower in non‐infiltrated leaf samples where light scattering increased the light gradient. The fluorescence profile was broader when the leaf was irradiated on its adaxial versus abaxial surface due to the contrasting optical properties of the palisade and spongy mesophyll. Irradiation with blue, red and green monochromatic light produced profiles that peaked 50, 100 and 150 μm, respectively, beneath the irradiated surface. These results are consistent with previous measurements of the light gradient in spinach and they agree qualitatively with measurements of carbon fixation under monochromatic blue, red and green light. These results suggest that chlorophyll fluorescence profiles may be used to estimate the distribution of quanta that are absorbed within the leaf for photosynthesis. 相似文献
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Photosynthesis and photoinhibition in leaves of chlorophyll b-less barley in relation to absorbed light 总被引:3,自引:0,他引:3
The response of photosynthesis to absorbed light by intact leaves of wild-type ( Hordeum vulgare L. cv. Gunilla) and chlorophyll b -less barley ( H. vulgare L. cv. Dornaria, chlorina-f22800 ) was measured in a light integrating sphere. Up to the section where the light response curve bends most sharply the responses of the b -less and wild-type barley were similar but not identical. Average quantum yield and convexity for the mutant light response curves were 0.89 and 0.90, respectively, times those of the wild-type barley. The maximum quantum yield for PSII photochemistry was also 10% lower as indicated by fluorescence induction kinetics (Fv /Fm ). Just above the region where the light curve bends most sharply, photosynthesis decreased with time in the mutant but not in the wild-type barley. This decrease was associated with a decrease in Fv /Fm indicating photoinhibition of PSII. This photoinhibition occurred in the same region of the light response curve where zeaxanthin formation occurs. Zeaxanthin formation occurred in both the chlorophyll b -less and wild-type leaves. However, the epoxidation state was lower in the mutant than in the wild-type barley. The results indicate that chlorophyll b -less mutants will have reduced photosynthetic production as a result of an increased sensitivity to photoinhibition and possibly a lowered quantum yield and convexity in the absence of photoinhibition. 相似文献
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Rapid light curves: A new fluorescence method to assess the state of the photosynthetic apparatus 总被引:35,自引:4,他引:35
Photosynthetic electron transport rates (ETR), calculated from chlorophyll fluorescence parameters, were compared in long term light and dark adapted as well as photoinhibited Pisum sativum leaves using a novel chlorophyll fluorescence method and a new instrument: rapid light curves (RLC) generated with the MINI-PAM. RLCs are plots of ETRs versus actinic irradiances applied for 10 s. Large changes in maximum electron transport rates (ETRmax) were observed when leaves were shifted from dark to moderate light, or from dark to photoinhibitory light and vice versa. Maximum ETRs were very low following long term dark adaptation, but increased to maximum levels within 8 to 15 minutes of illumination. It took more than 3 hours, however, to return irradiance-exposed leaves to the fully dark adapted state. Quenching analysis of RLCs revealed large qE development in long-term dark adapted leaves accounting for the low ETRs. Leaves photoinhibited for 3 hours had similarly reduced ETRs. In these leaves, however, qI was largely responsible for this reduction. Actinic irradiance exposures and saturating flashes affected leaves with different irradiance histories differently. 相似文献
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Photosynthetic rates and partitioning of absorbed light energy in photoinhibited leaves 总被引:9,自引:0,他引:9
Parameters for the evaluation of the effects of photoinhibition on photosynthetic carbon gain were studied in Chenopodium album leaves. The light-response curve of photosynthetic rate was determined at 36 Pa CO2 partial pressure and fitted by a non-rectangular hyperbola. Both the initial slope of the curve and the light-saturated rate decreased in photoinhibited leaves, although the decrease in the latter was small. The convexity of the curve was also smaller in photoinhibited leaves. The capacities of ribulose-1,5-bisphosphate carboxylation ( V cmax ) and electron transport ( J max ) were estimated from the CO2 -response curves. V cmax and J max decreased similarly with increasing photoinhibition. Energy partitioning in photosystem II (PSII) was estimated using chlorophyll fluorescence parameters. The fraction of energy that was consumed by photochemistry decreased with increasing photoinhibition. However, an increase in inactive PSII, decreasing energy partitioning to active PSII, relaxed the excitation pressure in PSII, and led to a reduction in the fraction of excess energy that was neither consumed by photochemistry nor dissipated as heat. 相似文献
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The quenching action of dibromothymoquinone on fluorescence and on primary photochemistry was examined in chloroplasts at ?196 °C. Both the initial (F0) and final (FM) levels of fluorescence as well as the fluorescence of variable yield (Fv = FM ? F0) were quenched at ?196 °C to a degree which depended on the concentration of dibromothymoquinone added prior to freezing. The initial rate of photoreduction of C-550 at — 196 °C, which was assumed to be proportional to maximum yield for primary photochemistry, ?Po, was also decreased in the presence of dibromothymoquinone. Simple theory predicts that the ratio should equal ?Po. Excellent agreement was found in a comparison of relative values of ?Po with relative values of at various degrees of quenching by dibromothymoquinone. These results are taken to indicate that F0 and FV are the same type of fluorescence, both emanating from the bulk chlorophyll of Photosystem II.Dibromothymoquinone appears to create quenching centers in the bulk chlorophyll of Photosystem II which compete with the reaction centers for excitation energy. The rate constant for the quenching of excitation energy by dibromothymoquinone is directly proportional to the concentration of the quencher. Rate constants for the de-excitation of excited chlorophyll molecules by fluorescence, kF, by nonradiative decay processes, kD, by photochemistry, kP, and by the specific quenching of dibromothymoquinone, kQ, were calculated assuming the absolute yield of fluorescence at F0 to be either 0.02 or 0.05. 相似文献
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Domagoj ?imi? Hrvoje Lepedu? Vlatka Jurkovi? Jasenka Antunovi? Vera Cesar 《Acta Botanica Sinica》2014,(7)
Chlorophyll fluorescence transient from initial to maximum fluorescence(P step) throughout two intermediate steps(J and I)(JIP‐test) is considered a reliable early quantitative indicator of stress in plants. The JIP‐test is particularly useful for crop plants when applied in variable field environments. The aim of the present study was to conduct a quantitative trait loci(QTL) analysis for nine JIP‐test parameters in maize during flowering in four field environments differing in weather conditions. QTL analysis and identification of putative candidate genes might help to explain the genetic relationship between photosynthesis and different field scenarios in maize plants. The JIP‐test parameters were analyzed in the intermated B73 Mo17(IBM) maize population of 205 recombinant inbred lines. A set of 2,178 molecular markers across the whole maize genome was used for QTL analysis revealing 10 significant QTLs for seven JIP‐test parameters, of which five were co‐localized when combinedover the four environments indicating polygenic inheritance and pleiotropy. Our results demonstrate that QTL analysis of chlorophyll fluorescence parameters was capable of detecting one pleiotropic locus on chromosome 7, coinciding with the gene gst23 that may be associated with efficient photosynthesis under different field scenarios. 相似文献
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The chlorophyll a fluorescence in Chlorella pyrenoidosa can be enhanced by 4–9% if the excitation light beam is parallel to an external magnetic field or decreased by 4–9% if the light beam is oriented perpendicular to a magnetic field of about 16 kgauss or more. These effects cannot be explained in terms of the small changes in light absorption which are also observed. It is suggested that these observations are due to a reorientation of pigment molecules in the magnetic field. 相似文献
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Despite the recognized importance of the multi-scale spatio-temporal organization of proteins, most computational tools can only access a limited spectrum of time and spatial scales, thereby ignoring the effects on protein behavior of the intricate coupling between the different scales. Starting from a physico-chemical atomistic network of interactions that encodes the structure of the protein, we introduce a methodology based on multi-scale graph partitioning that can uncover partitions and levels of organization of proteins that span the whole range of scales, revealing biological features occurring at different levels of organization and tracking their effect across scales. Additionally, we introduce a measure of robustness to quantify the relevance of the partitions through the generation of biochemically-motivated surrogate random graph models. We apply the method to four distinct conformations of myosin tail interacting protein, a protein from the molecular motor of the malaria parasite, and study properties that have been experimentally addressed such as the closing mechanism, the presence of conserved clusters, and the identification through computational mutational analysis of key residues for binding. 相似文献
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《Journal of microbiological methods》1987,6(4):199-210
An IBM-PC based software system to quantify images of biological sytems is presented. To illustrate the potential of the imaging system described, UV light-induced chlorophyll fluorescence of Chlorella vulgaris cells was monitored using a video camera interfaced to a microscope. The photoinhibitor, DCMU (30 μM) inhibited the UV-induced fluorescence decay in heterotrophically grown cells but not in autotrophically grown cells. Algal cells were also incubated in various concentrations of ascorbic acid (500, 250, 100, 50, 10 and 0 mM) prior to UV light exposure. In the presence of 500, 250 and 100 mM ascorbic acid, the decay of chlorophyll fluorescence was significantly delayed in both heterotrophically and autotrophically grown cells. Heterotrophically grown cells were more sensitive to ascorbic acid than autotrophically grown cells since 10 nM ascorbic acid delayed fluorescence decay in heterotrophic cultures. 相似文献
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A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts. 总被引:22,自引:0,他引:22
A detailed study of the photo-induced decline in chlorophyll a fluorescence intensity (Kautsky phenomenon) in coupled isolated chloroplasts from a high level (P) to a low stationary level (S) is presented. 1. A linear relationship between P leads to S quenching and intrathylakoid H+ concentration was found. When the light-induced proton gradient was abolished by uncoupling, the fluorescence emission at room temperature was lowered proportionally to increased H+ concentration in the medium. 2. Fluorescence spectra at -196 degrees C of samples frozen at the P and S states showed no significant differences in the Photosystem I/Photosystem II ratio of fluorescence emission. Furthermore, freezing to -196 degrees C reversed the P leads to S quenching. This indicates that the P leads to S quenching is not related to an increase of spillover of excitation energy from Photosystem II to Photosystem I. 3. When Mg2+ was added to thylakoids suspended in a medium free of divalent cations, the inhibition of spillover required lower Mg2+ concentrations (half saturation at 0.6 mM). Increased proton concentration in the medium also inhibited spillover. 4. The results are interpreted in terms of two sites of Mg2+ and H+ effects on excitation deactivation in Photosystem II. One site is located on the outer face of the thylakoid membrane; action of both Mg2+ and H+ at this side diminishes spillover. The second site is located on the inner face of the membrane; as Mg2+ is displaced there by protons, a non-photochemical quenching of Photosystem II fluorescence is induced, which is manifested by the P leads to S decline. 相似文献
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Chlorophyll-a contained in the peel of Granny Smith apples emits fluorescence upon excitation with blue light. The observed emission, collected by an external detector and corrected by its spectral response, is still distorted by light reabsorption processes taking place in the fruit skin and differs appreciably from the true spectral distribution of fluorescence emerging from chlorophyll molecules in the biological tissue. Reabsorption processes particularly affect the ratio of fluorescence intensities at 680 nm and at 730 nm. A model to obtain the correct spectral distribution of the emission, from the experimental fluorescence recorded at a fluorometer detector and corrected for the detector spectral sensitivity, is developed in the present work. Measurements of the whole fruit reflectance, the peel transmittance and the flesh reflectance allow the calculation of the reabsorption-corrected spectra. The model is validated by comparing the corrected emission spectra with that obtained for a thin layer of apple-peel-chloroplasts, where no reabsorption takes place. It is recommended to correct distortions in emission spectra of intact fruits due to light reabsorption effects whenever a correlation between the physiological state of the fruit and its fluorescence spectra is investigated. 相似文献
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Chilling tolerance in eight taxa was estimated from field and controlled environment observations and compared to tolerance estimated using a variety of techniques. The controlled environment observations suggested that the eight taxa could be ranked from chilling tolerant to chilling sensitive in the order: pea ( Pisum sativum L. cv. Greenfeast), Passiflora edulis Sims., Passiflora ligularis Juss. and Passiflora quadrangularis L., pepino ( Solanum muricatum L.) cultivars Comeraya, Suma, Miski, and mungbean [ Vigna radiata (L.) R. Wilcz]; although the relationship between the passionfruit as a group and the pepinos was unclear.
The change in the variable component of chlorophyll fluorescence (FR ) with time near 0°C in darkness was the most reliable method of ranking the plants according to relative chilling tolerance. It was also sufficiently sensitive to discriminate clearly between the closely related pepino cultivars. The Passiflora species and pea were not susceptible to short term reductions in FR , with or without a 20 min exposure to light. Exposure to light at temperatures near 0°C emphasised the reductions in FR in the more sensitive species. Pea was the only species capable of recovering a measurable FR after a 60 min exposure to white light.
Measurement of electrolyte leakage and ethylene evolution from leaf disks after a low temperature treatment could allow discrimination between closely related varieties, but not between genera. Catalase activity was reduced in all taxa in response to low temperature. However, both initial catalase levels and relative response to dark treatment at 20°C enabled the ranking of plants within the closely related subgroups according to susceptibility to chill injury.
No one method clearly distinguished chilling sensitivity over all taxa. 相似文献
The change in the variable component of chlorophyll fluorescence (F
Measurement of electrolyte leakage and ethylene evolution from leaf disks after a low temperature treatment could allow discrimination between closely related varieties, but not between genera. Catalase activity was reduced in all taxa in response to low temperature. However, both initial catalase levels and relative response to dark treatment at 20°C enabled the ranking of plants within the closely related subgroups according to susceptibility to chill injury.
No one method clearly distinguished chilling sensitivity over all taxa. 相似文献