共查询到20条相似文献,搜索用时 15 毫秒
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Rival-Gervier S Pantano T Viglietta C Maeder C Prince S Attal J Jolivet G Houdebine LM 《Transgenic research》2003,12(6):723-730
Previous studies have shown that the 5'HS4 DNaseI hypersensitive site of the chicken beta-globin locus is endowed with classic insulator activities: (i) it blocks the interaction between promoter and enhancers when it is inserted between them (ii) it confers expression of integrated foreign genes independent of their position in the chromatin. The aim of this present work was to determine whether the 5'HS4 element was able to stimulate the expression level and/or to increase the expression frequency of a luc+ reporter gene controlled by the rabbit WAP gene promoter. Two constructs with 5'HS4 insulator (p5'HS4-WAPluc) or without (pWAPluc) were introduced in mouse fertilised oocytes. All transgenic lines containing the 5'HS4 element (six lines) expressed the transgene whereas only two out of eight lines harbouring the pWAP-luc construct expressed the transgene to a significant level. Moreover, the mean level of expression was seven times higher in p5'HS4WAP-luc lines than in pWAP-luc lines. Even all these benefits on transgene expression, the 5'HS4 element did not confer a copy-dependent expression, did not decrease the ectopic expression of the reporter gene and did not decrease the variability of expression. Thus, the 5'HS4 element does not have all the properties of a perfect insulator on a construct containing the luc+ reporter gene controlled by the rabbit WAP promoter. 相似文献
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Charlotte B. Jendresen Hao Cui Xiao Zhang Israel Vlodavsky Lars N. G. Nilsson Jin-Ping Li 《The Journal of biological chemistry》2015,290(8):5053-5064
Heparan sulfate (HS) and HS proteoglycans (HSPGs) colocalize with amyloid-β (Aβ) deposits in Alzheimer disease brain and in Aβ precursor protein (AβPP) transgenic mouse models. Heparanase is an endoglycosidase that specifically degrades the unbranched glycosaminoglycan side chains of HSPGs. The aim of this study was to test the hypothesis that HS and HSPGs are active participators of Aβ pathogenesis in vivo. We therefore generated a double-transgenic mouse model overexpressing both human heparanase and human AβPP harboring the Swedish mutation (tgHpa*Swe). Overexpression of heparanase did not affect AβPP processing because the steady-state levels of Aβ1–40, Aβ1–42, and soluble AβPP β were the same in 2- to 3-month-old double-transgenic tgHpa*Swe and single-transgenic tgSwe mice. In contrast, the Congo red-positive amyloid burden was significantly lower in 15-month-old tgHpa*Swe brain than in tgSwe brain. Likewise, the Aβ burden, measured by Aβx-40 and Aβx-42 immunohistochemistry, was reduced significantly in tgHpa*Swe brain. The intensity of HS-stained plaques correlated with the Aβx-42 burden and was reduced in tgHpa*Swe mice. Moreover, the HS-like molecule heparin facilitated Aβ1–42-aggregation in an in vitro Thioflavin T assay. The findings suggest that HSPGs contribute to amyloid deposition in tgSwe mice by increasing Aβ fibril formation because heparanase-induced fragmentation of HS led to a reduced amyloid burden. Therefore, drugs interfering with Aβ-HSPG interactions might be a potential strategy for Alzheimer disease treatment. 相似文献
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《Cell communication & adhesion》2013,20(4):359-375
α4 integrins (α4β1 and α4β7) have been shown to mediate both cell-matrix adhesion to fibronectin and cell-cell adhesion to VCAM-1. These interactions have been suggested to contribute to hematopoiesis, lymphocyte homing, recruitment of inflammatory cells, neural crest cell migration and myogenesis. We report here the cloning of chicken α4 cDNA and its use to define the patterns of expression of α4 mRNA and protein in early chicken embryos (19–22 somite pairs), a stage at which neural crest cells can be examined at various points in their migration and somitic development and differentiation can also be observed at various stages. We observe widespread expression of both α4 mRNA and protein, although the patterns of steady state expression do not conform precisely. Many neural crest cells contain significant levels of α4 mRNA. Some neural crest cells express α4 protein but its expression is transient and/or limited to a subset of these cells. α4 is strongly expressed at both mRNA and protein levels by somitic cells and their derivatives in the sclerotome, dermatome and myotome and is also expressed in neural tube, otic placode, heart, gut endoderm and some other tissues. Comparison with the distributions of fibronectin shows that, although some α4 expression occurs in locations consistent with a role in cell-matrix adhesion to fibronectin, α4 is also expressed in other places where fibronectin is low or absent and a role for α4 in cell-cell interactions appears more likely. 相似文献
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Arregui L Benítez JA Razgado LF Vergara P Segovia J 《Cellular and molecular neurobiology》2011,31(8):1229-1243
Huntington’s disease (HD) is a neurodegenerative disorder characterized by motor, cognitive, and psychiatric symptoms. The
most characteristic structural feature of this disease is neurodegeneration accompanied by gliosis in the striatum. BDNF has
been proposed to protect striatal neurons from degeneration, because it is an important survival factor for these neurons
from development to adulthood. Considering the extensive gliosis and the survival effects of BDNF, we constructed an adenovirus
to express a BDNF cDNA in astrocyte cells using a promoter of the glial fibrillary acidic protein gene. Cells stably transfected
in vitro with a BDNF cDNA driven by this promoter expressed BDNF and responded to external stimuli increasing BDNF production.
When the vector was applied into the striata of mice transgenic for HD, long-term expression of the transgene was observed,
associated with a delay of onset of the motor phenotype of the R6/2 HD transgenic mice. The present data indicate that the
striatal expression of BDNF is a potential adjuvant for the treatment of HD. 相似文献
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Michael G. Spelios John A. Olsen Lauren A. Kenna Eitan M. Akirav 《The Journal of biological chemistry》2015,290(24):15250-15259
The co-culturing of insulinoma and islet-derived endothelial cell (iEC) lines results in the spontaneous formation of free-floating pseudoislets (PIs). We previously showed that iEC-induced PIs display improved insulin expression and secretion in response to glucose stimulation. This improvement was associated with a de novo deposition of extracellular matrix (ECM) proteins by iECs in and around the PIs. Here, iEC-induced PIs were used to study the expression and posttranslational modification of the ECM receptor integrin β1. A wide array of integrin β subunits was detected in βTC3 and NIT-1 insulinomas as well as in primary islets, with integrin β1 mRNA and protein detected in all three cell types. Interestingly, the formation of iEC-induced PIs altered the glycosylation patterns of integrin β1, resulting in a higher molecular weight form of the receptor. This form was found in native pancreas but was completely absent in monolayer β-cells. Fluorescence-activated cell sorting analysis of monolayers and PIs revealed a higher expression of integrin β1 in PIs. Antibody-mediated blocking of integrin β1 led to alterations in β-cell morphology, reduced insulin gene expression, and enhanced glucose secretion under baseline conditions. These results suggest that iEC-induced PI formation may alter integrin β1 expression and posttranslational modification by enhancing glycosylation, thereby providing a more physiological culture system for studying integrin-ECM interactions in β cells. 相似文献
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Background
The eye lens is composed of fiber cells that are filled with α-, β- and γ-crystallins. The primary function of crystallins is to maintain the clarity of the lens through ordered interactions as well as through the chaperone-like function of α-crystallin. With aging, the chaperone function of α-crystallin decreases, with the concomitant accumulation of water-insoluble, light-scattering oligomers and crystallin-derived peptides. The role of crystallin-derived peptides in age-related lens protein aggregation and insolubilization is not understood.Methodology/Principal Findings
We found that αA-crystallin-derived peptide, 66 SDRDKFVIFLDVKHF 80, which accumulates in the aging lens, can inhibit the chaperone activity of α-crystallin and cause aggregation and precipitation of lens crystallins. Age-related change in the concentration of αA-(66-80) peptide was estimated by mass spectrometry. The interaction of the peptide with native crystallin was studied by multi-angle light scattering and fluorescence methods. High molar ratios of peptide-to-crystallin were favourable for aggregation and precipitation. Time-lapse recordings showed that, in the presence of αA-(66-80) peptide, α-crystallin aggregates and functions as a nucleus for protein aggregation, attracting aggregation of additional α-, β- and γ-crystallins. Additionally, the αA-(66-80) peptide shares the principal properties of amyloid peptides, such as β-sheet structure and fibril formation.Conclusions/Significance
These results suggest that crystallin-derived peptides such as αA-(66-80), generated in vivo, can induce age-related lens changes by disrupting the structure and organization of crystallins, leading to their insolubilization. The accumulation of such peptides in aging lenses may explain a novel mechanism for age-related crystallin aggregation and cataractogenesis. 相似文献11.
Zinc protoporphyrin (ZnPP) has been found to have anticancer activity both in vitro and in vivo. We have recently demonstrated that ZnPP diminishes β-catenin protein expression in cancer cells. The present study examined the cellular mechanisms that mediate ZnPP’s suppression of β-catenin expression. We demonstrate that ZnPP induces a rapid degradation of the β-catenin protein in cancer cells, which is accompanied by a significant inhibition of proteasome activity, suggesting that proteasome degradation does not directly account for the suppression. The possibility that ZnPP induces β-catenin exportation was rejected by the observation that there was no detectable β-catenin protein in the conditioned medium after ZnPP treatment of cancer cells. Further experimentation demonstrated that ZnPP induces lysosome membrane permeabilization, which was reversed by pretreatment with a protein transportation inhibitor cocktail containing Brefeldin A (BFA) and Monensin. More significantly, pretreatment of cancer cells with BFA and Monensin attenuated the ZnPP-induced suppression of β-catenin expression in a concentration- and time-dependent manner, indicating that the lysosome protein degradation pathway is likely involved in the ZnPP-induced suppression of β-catenin expression. Whether there is cross-talk between the ubiquitin-proteasome system and the lysosome pathway that may account for ZnPP-induced β-catenin protein degradation is currently unknown. These findings provide a novel mechanism of ZnPP’s anticancer action and reveal a potential new strategy for targeting the β-catenin Wnt signaling pathway for cancer therapy. 相似文献
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Marcus Dorner Franziska Zucol Davide Alessi Stephan K. Haerle Walter Bossart Markus Weber Rahel Byland Michele Bernasconi Christoph Berger Sharof Tugizov Roberto F. Speck David Nadal 《Journal of virology》2010,84(13):6667-6677
Epstein-Barr virus (EBV) uses nasal mucosa-associated lymphoid tissue (NALT) as a portal of entry to establish life-long persistence in memory B cells. We previously showed that naïve and memory B cells from NALT are equally susceptible to EBV infection. Here we show that memory B cells from NALT are significantly more susceptible to EBV infection than those from remote lymphatic organs. We identify β1 integrin, which is expressed the most by naïve B cells of distinct lymphoid origin and by memory B cells from NALT, as a mediator of increased susceptibility to infection by EBV. Furthermore, we show that BMRF-2-β1 integrin interaction and the downstream signal transduction pathway are critical for postbinding events. An increase of β1 integrin expression in peripheral blood memory B cells provoked by CD40 stimulation plus B-cell receptor cross-linking increased the susceptibility of non-NALT memory B cells to EBV infection. Thus, EBV seems to utilize the increased activation status of memory B cells residing in the NALT to establish and ensure persistence.Epstein-Barr virus (EBV) is a ubiquitous human gammaherpesvirus that is transmitted via saliva and infects more than 90% of the world''s population (21). Much of EBV''s medical importance relates to its association with B-cell malignancies, including Burkitt''s lymphoma, Hodgkin''s lymphoma, and posttransplant lymphoproliferative disease (21). The oncogenic potential of EBV is clearly illustrated by its unique capability to transform B cells in vitro (21).In the current paradigm, EBV infects naïve B cells in tonsils in vivo (32). EBV is present mainly as a latent virus; upon infection, EBV expresses distinct patterns of its latency genes depending upon distinct B-cell differentiation stages, varying from expression of all 10 known EBV latency genes in naïve B cells to the complete absence of EBV mRNA expression in resting memory B cells. This has led to the model that EBV, by virtue of expression of its latency genes, provides cell survival signals in naïve B cells (32). In particular, recent data suggest that EBV expedites the antigen-driven somatic hypermutation and selection of B cells taking place in germinal centers (GC) (26). Chaganti et al. challenged the current paradigm by showing for patients with primary EBV infection that EBV avoids GC transit and directly infects memory B cells (6). This report is consistent with in vitro experiments showing that EBV is able to infect memory B cells (9, 10), in addition to the well-accepted susceptibility of naïve and GC B cells to EBV.Irrespective of which B-cell subset is the primary target of EBV, its propagation within the host is linked to proliferation of infected B cells, which deliver latent EBV to daughter cells, or, more rarely, to switching of EBV to lytic infection (21). The latter process can eventually be triggered by the differentiation of infected memory B cells into plasma cells and results in the release of virions that may subsequently infect new B cells (17). Importantly, transmission of EBV to naïve hosts is thought to occur via droplets loaded with virions (21). Thus, lytic replication of EBV takes place best in nasal mucosa-associated lymphoid tissue (NALT), which will release EBV into the saliva, generating infectious droplets. Therefore, the NALT is the point of EBV transmission, i.e., the portal of entry of EBV as well as a shedding organ for further transmission (21).The attachment of EBV to B cells is mediated by the direct interaction of EBV glycoprotein gp350/220 with cellular CD21, initiating receptor-mediated endocytosis. After binding to CD21, EBV gp42 can interact with host HLA class II molecules, leading to a conformational change in the viral glycoproteins and triggering fusion with the host cell membrane (12, 28). Nevertheless, experimental data suggest that CD21 and HLA class II molecules are dispensable for the infection of B cells (14). Notably, in polarized oropharyngeal epithelial cells, which lack CD21, interactions between β1 integrin and the EBV glycoprotein BMRF-2 via its Arg-Gly-Asp (RGD) motif are critical for infection (34, 38, 39). The role of β1 integrin in mediating EBV infection of memory B cells from NALT or non-NALT is unknown.We recently demonstrated that tonsillar memory B cells are much more susceptible to EBV infection than those from the peripheral blood, originating from various lymphoid tissues (9). Thus, tonsillar memory B cells seem to express properties which render them more susceptible to EBV infection than their counterparts of other lymphatic origin.Here we hypothesized that memory B cells from the NALT exhibit specific properties rendering them highly susceptible to EBV infection. Indeed, in this work, we found that memory B cells from the NALT are distinguishable from memory B cells of other lymphoid tissue by their β1 integrin expression levels, and thus their activation status, and that this higher expression level is a critical factor in their greater susceptibility to EBV infection. 相似文献
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Eric A. Tanifum Indrani Dasgupta Mayank Srivastava Rohan C. Bhavane Li Sun John Berridge Hoda Pourgarzham Rashmi Kamath Gabriela Espinosa Stephen C. Cook Jason L. Eriksen Ananth Annapragada 《PloS one》2012,7(10)
Extracellular amyloid-β (Aβ) plaques and intracellular neurofibrillary tangles constitute the major neuropathological hallmarks of Alzheimer’s disease (AD). It is now apparent that parenchymal Aβ plaque deposition precedes behavioral signs of disease by several years. The development of agents that can target these plaques may be useful as diagnostic or therapeutic tools. In this study, we synthesized an Aβ-targeted lipid conjugate, incorporated it in stealth liposomal nanoparticles and tested their ability to bind amyloid plaque deposits in an AD mouse model. The results show that the particles maintain binding profiles to synthetic Aβ aggregates comparable to the free ligand, and selectively bind Aβ plaque deposits in brain tissue sections of an AD mouse model (APP/PSEN1 transgenic mice) with high efficiency. When administered intravenously, these long circulating nanoparticles appear to cross the blood-brain barrier and bind to Aβ plaque deposits, labeling parenchymal amyloid deposits and vascular amyloid characteristic of cerebral amyloid angiopathy. 相似文献
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Mutations in presenilin 1 (PS-1) are associated with most early-onset familiar Alzheimer’s disease (AD). Previous studies
have demonstrated that PS-1 mutations enhance the production of beta-amyloid (Aβ). In this study, we further examined the
in vivo effects of PS-1 mutation on tau and synapse protein markers. The data showed that the phosphorylation of tau at Ser396,
Ser404, Thr231 and Tau-1 (Ser198/199/202) epitopes was significantly increased in hippocampus of the aged (twenty-one and
a half-month-old) transgenic mice expressing PS-1 (L235P) compared to that of the age-matched wild-type littermates (WTs).
Concurrently, a significant decrease in the phosphorylation of glycogen synthase kinase (GSK)-3β at Ser9 was observed. The
above changes were not observed in the young transgenic mice (6–8 months old). No significant changes in the levels of cyclin-dependent
kinase (CDK)-5, its co-activator p35, and phosphorylation of protein phosphatase (PP)-2A catalytic subunit at Tyrosine 307
(Y307), a crucial site regulating the activity of PP-2A, were observed both in the young and aged transgenic mice compared
to that of WTs. Furthermore, we also observed that the levels of presynaptic synaptophysin were significantly decreased but
postsynaptic density protein (PSD)-95 were not significantly altered in hippocampus of the aged transgenic mice. No significant
changes of synaptophysin or PSD-95 were observed in the brains of the young transgenic mice. Our data indicate that the L235P
PS-1 mutation can induce Alzheimer-like tau hyperphosphorylation and synaptic protein loss, as well as increased production
of Aβ. 相似文献
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Lan Zhang Cong Shen Jin Chu Ruyi Zhang Yali Li Lin Li 《International journal of biological sciences》2014,10(2):181-191
Objective: The purpose of this study was to investigate the effects and pharmacological mechanisms of icariin, which is the main component in the traditional Chinese herb Epimedium, on β-amyloid (Aβ) production in an amyloid precursor protein (APP) transgenic (Tg) mouse model of Alzheimer''s disease (AD).Methods: APPV717I Tg mice were randomly divided into a model group and icariin-treated (30 and 100 μmol/kg per day) groups. Learning-memory abilities were determined by Morris water maze and object recognition tests. Aβ contents were measured by enzyme-linked immunosorbent assays and immunohistochemistry. Amyloid plaques were detected by Congo red staining and Bielschowsky silver staining. The levels of expression of APP and β-site APP-cleaving enzyme 1 (BACE-1) were measured by western blotting and immunohistochemistry.Results: Ten-month-old Tg mice showed obvious learning-memory impairments, and significant increases in Aβ contents, amyloid plaques, and APP and BACE-1 levels in the hippocampus. The intragastric administration of icariin to Tg mice for 6 months (from 4 to 10 months of age) improved the learning-memory abilities and significantly decreased the Aβ contents, amyloid plaques, and APP and BACE-1 levels in the hippocampus.Conclusion: Icariin reduced the Aβ burden and amyloid plaque deposition in the hippocampus of APP transgenic mice by decreasing the APP and BACE-1 levels. These novel findings suggest that icariin may be a promising treatment in patients with AD. 相似文献
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Smita Zaheer Ramasamy Thangavel Yanghong Wu Mohammad Moshahid Khan Duraisamy Kempuraj Asgar Zaheer 《Neurochemical research》2013,38(1):218-225
We previously demonstrated that glia maturation factor (GMF), a brain specific protein, isolated, sequenced and cloned in our laboratory, induce expression of proinflammatory cytokines and chemokines in the central nervous system. We also reported that the up-regulation of GMF in astrocytes leads to the destruction of neurons suggesting a novel pathway of GMF-mediated cytotoxicity of brain cells, and implicated its involvement in the pathogenesis of inflammatory neurodegenerative diseases. In the present study, we examined the expressions of GMF in triple-transgenic Alzheimer’s disease (3xTg-AD) mice. Our results show a 13-fold up-regulation of GMF and 8–12-fold up-regulation of proinflammatory cytokines tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), IL-1β, interferon gamma (IFN-γ), and chemokine (C–C motif) ligand 2 (CCL2) and C–X–C motif chemokine 10 (CXCL10/IP-10) mRNA as determined by quantitative real-time RT-PCR in the brain of 3xTg-AD mice as compared to non-transgenic (Non-Tg) mice. In conclusion, the increase in GMF and cytokine/chemokine expression was correlated with reactive glial fibrillary acidic protein positive astrocytes and ionized calcium binding adaptor molecule 1 (Iba-1)-positive microglia in 3xTg-AD mice. 相似文献
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Rizwan S. Akhtar Barbara J. Klocke Andreas Strasser Kevin A. Roth 《The journal of histochemistry and cytochemistry》2008,56(10):921-927
Members of the Bcl-2 family include pro- and antiapoptotic proteins that regulate programmed cell death of developing tissues and death in response to cellular damage. In developing mice, the antiapoptotic Bcl-xL is necessary for survival of neural and hematopoietic cells, and consequently, bcl-x–deficient mice die around Day 13.5 of embryogenesis. Furthermore, adult bcl-x+/− heterozygous male mice have reduced fertility because of testicular degeneration. Bax, a multi-BH (Bcl-2 homology) domain proapoptotic member of the Bcl-2 family, is regulated by Bcl-xL and is required for the neuropathological abnormalities seen in bcl-x–deficient embryos. The BH3 domain only subgroup of the Bcl-2 family includes proapoptotic members that are essential for the initiation of apoptotic signaling. In this study, we investigated the role for Bim, a BH3 domain only protein, in the embryonic lethality and increased developmental cell death in bcl-x–deficient animals and the perturbed testicular function in bcl-x+/− adults. Our studies show that bim deficiency attenuates hematopoietic cell death in the fetal liver of bcl-x–deficient animals, indicating that Bim contributes to programmed cell death in this cell population. In addition, we found that testicular degeneration of adult bcl-x+/− males was rescued by concomitant Bim deficiency. However, concomitant Bim deficiency had no effect on the embryonic lethality and widespread nervous system abnormalities caused by bcl-x deficiency. Our work identifies Bim as an important regulator of bcl-x deficiency–induced cell death during hematopoiesis and testicular development. (J Histochem Cytochem 56:921–927, 2008) 相似文献
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Qian Liu Yao JinKun Wang Xiang-Xue MengYang Yang Zhuo YangYong-Shan Zhao Ming-Yi ZhaoJing-Hai Zhang 《Comptes rendus biologies》2014,337(2):73-77
The voltage-gated sodium channel (VGSC) is a complex, which is composed of one pore-forming α subunit and at least one β subunit. Up to now, five β subunits are known: β1/β1A, β1B, β2, β3, and β4, encoded by four genes (SCN1B∼SCN4B). It is critical to have a deep understanding of the interaction between β1 and β3 subunits, two subunits which frequently appear in many diseases concurrently. In this study, we had screened out the new template of β1 subunit for homology modelling, which shares higher similarity to β3. Docking studies of the β1 and β3 homology model were conducted, and likely β1 and β3 binding loci were investigated. The results revealed that β1–β3 is more likely to form a di-polymer than β1–β1 based on molecular interaction analysis, including potential energy analysis, Van der Waals (VDW) energy analysis and electrostatic energy analysis, and in addition, consideration of the hydrogen bonds and hydrophobic contacts that are involved. Based on these analyses, the residues His122 and Lys140 of β1 and Glu 66, Asn 131, Asp 118, Glu 120, Glu133, Asn135, Ser 137 of β3 were predicted to play a functional role. 相似文献
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Chunli Shao Michael C. Lawrence Melanie H. Cobb 《The Journal of biological chemistry》2010,285(26):19710-19719
Apoptosis contributes to immune-mediated pancreatic β cell destruction in type I diabetes. Exposure of β cells to interleukin-1β (IL-1β) causes endoplasmic reticulum stress and activates proapoptotic networks. Here, we show that nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways regulate the expression of CCAAT/enhancer-binding protein homologous protein (CHOP), which mediates endoplasmic reticulum stress-induced apoptosis. Both CHOP mRNA and protein increase in β cells treated with IL-1β. In addition, prolonged exposure to high glucose further increases IL-1β-triggered CHOP expression. IL-1β also causes increased expression of C/EBP-β and a reduction of MafA, NFATc2, and Pdx-1 expression in β cells. Inhibition of the NF-κB and MAPK signaling pathways differentially attenuates CHOP expression. Knocking down CHOP by RNA interference protects β cells from IL-1β-induced apoptosis. These studies provide direct mechanistic links between cytokine-induced signaling pathways and CHOP-mediated apoptosis of β cells. 相似文献