共查询到20条相似文献,搜索用时 15 毫秒
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GATA-4 and GATA-5 transcription factor genes and potential downstream antitumor target genes are epigenetically silenced in colorectal and gastric cancer 总被引:7,自引:0,他引:7 下载免费PDF全文
Akiyama Y Watkins N Suzuki H Jair KW van Engeland M Esteller M Sakai H Ren CY Yuasa Y Herman JG Baylin SB 《Molecular and cellular biology》2003,23(23):8429-8439
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The roles of GATA-4, -5 and -6 in vertebrate heart development 总被引:15,自引:0,他引:15
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Nakagawa R Sakai Y Takashima A Terada T Kobayashi A Maeda M 《Biochemical and biophysical research communications》2001,283(2):412-416
A nuclear extract of the mouse I-10 Leydig tumor cell line was analyzed by gel mobility shift assay with a combination of antibodies for various mammalian GATA proteins. Antibodies for GATA-4 caused a super-shift of the DNA-protein complex, which is formed through GATA-4 binding to an oligonucleotide with a typical GATA motif, while ones for GATA-1, GATA-2, GATA-3, and GATA-6 did not. These results indicated that I-10 cells express GATA-4 protein. Western blotting analysis of cellular proteins also demonstrated the presence of GATA-4 protein, the size of which corresponds to that of the rat orthologous protein transiently expressed in Cos-1 cells. A significant level of GATA-4 expression in I-10 cells would be advantageous for studying the roles of this protein, especially in view of gonadal function. We further examined the binding site preference of GATA-4 expressed in I-10 cells. GATA-4 showed broad sequence specificity similar to GATA-6, the order of binding core site preference being GATA > GATT > GATC, and adenine was favored on both sides of the core for strong binding. Thus the conserved zinc finger domain of GATA proteins is suggested to contribute to the binding sequence preference. GATA-4 expressed in I-10 cells was not susceptible to proteolysis coupled with cAMP signaling. 相似文献
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Hey basic helix-loop-helix transcription factors are repressors of GATA4 and GATA6 and restrict expression of the GATA target gene ANF in fetal hearts 总被引:4,自引:0,他引:4 下载免费PDF全文
Fischer A Klattig J Kneitz B Diez H Maier M Holtmann B Englert C Gessler M 《Molecular and cellular biology》2005,25(20):8960-8970
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