‘Ins’ and ‘outs’ of triple therapy

Chronic oral anticoagulant treatment is obligatory in patients (class I) with mechanical heart valves and in patients with atrial fibrillation with CHADS2 score >1. When these patients undergo percutaneous coronary intervention with placement of a stent, there is also an indication for treatment with aspirin and clopidogrel. Unfortunately, triple therapy is known to increase the bleeding risk. For this group of patients, the bottom line is to find the ideal therapy in patients with indications for both chronic anticoagulation therapy and percutaneous intervention to prevent thromboembolic complications such as stent thrombosis without increasing the risk of bleeding. (Neth Heart J 2010;18:444-50.)     

Special Issue on‘‘Metagenomics of Marine Environments’’

<正>We are pleased to announce a special issue on‘‘Metagenomics of Marine Environments’’of the journal Genomics,ProteomicsBioinformatics(GPB),aiming to provide a platform for high-quality papers focusing on the topic and we invite submissions for this special issue(to be published in the fall of 2015).With most microbes being difficult or impossible to culture     

Structural and Narrative Reconstruction of Rural Residents’ Representations of ‘Nature’, ‘Wildlife’, and ‘Landscape’

The objective of this paper is the structural and narrative reconstruction of representations of ‘nature’, ‘wildlife’ and ‘landscape’, held by rural residents of the Dadia Forest Reserve. Data collection involved in-depth interviews. Employing a social representations’ approach, we recovered representational elements that are expected in the case of rural belief systems, such as negative dispositions towards wolves and foxes, as well as elements of an urban adherence, such as nature’s independence. Representational elements refer to visual aspects of the countryside, which seem compatible with the figurative nucleus of the rural idyll. Concerning ‘wildlife’, residents focused on vultures, which comprise the main tourist attraction of the reserve. Scientific knowledge adds to the complexity of the narrative schema, which corresponds to the representation of ‘wildlife’. Interviewees perceived the rural landscape as an interface between the natural and the human-conditioned environment. Our study shows that interviewees make no reference to environmental conservation or quality of life issues, as it could be expected according to relatively wide definitions of the term ‘environmentalism’. Environmental messages reinforced by ecotourism development seem to be recalled primarily in terms of their compatibility with the perceived economic benefit of local people. Despite ecotourism development, representational elements that diverge from a tourist version of ‘nature’, ‘wildlife’ and ‘landscape’ were not pronounced within rural belief-systems. Further interventions within the study area are needed, in order to address a variety of topics under the environmental conservation discourse and raise the environmental awareness of rural residents.     

Simulation of ‘hitch-hiking’ genealogies

An ancestral influence graph is derived, an analogue of the coalescent and a composite of Griffiths' (1991) two-locus ancestral graph and Krone and Neuhauser's (1997) ancestral selection graph. This generalizes their use of branching-coalescing random graphs so as to incorporate both selection and recombination into gene genealogies. Qualitative understanding of a ‘hitch-hiking’ effect on genealogies is pursued via diagrammatic representation of the genealogical process in a two-locus, two-allele haploid model. Extending the simulation technique of Griffiths and Tavaré (1996), computational estimation of expected times to the most recent common ancestor of samples of n genes under recombination and selection in two-locus, two-allele haploid and diploid models are presented. Such times are conditional on sample configuration. Monte Carlo simulations show that ‘hitch-hiking’ is a subtle effect that alters the conditional expected depth of the genealogy at the linked neutral locus depending on a mutation-selection-recombination balance. Received: 21 July 2000 / Published online: 5 December 2000     

The spectre of ‘spurious’ correlations

Summary Ecologists often standardize data through the use of ratios and indices. Such measures are employed generally to remove a size effect induced by some relatively uniteresting variable. The implications of using the resultant data in correlation and regression analyses are poorly recognized. We show that ratios and indices often provide surprising and spurious results due to their unusual properties. As a solution, we advocate the use of randomization tests to evaluate hypotheses confounded by spurious correlations. In addition, we emphasize that identifying the appropriate null correlation is of utmost importance when statistically evaluating ratios, although this issue is frequently ignored.     

The ‘curse’ of Westermarck 1

This article consists of a critical application of recent theory of nationalism to the unusual case of Mauritius. It is a poly‐ethnic and poly‐religious society whose inhabitants neither share a mythical distant past nor consider themselves culturally identical, but where nation‐building has nevertheless been moderately successful since the late 1960s. Through an examination of the processes of institutional and cultural nation‐building in the island, it is argued that the current Eurocentric view of the nation (notably as a culturally homogeneous imagined community) must be modified to fit cases like this one. Thus, the value of nationalism and nationhood as comparative concepts is questioned.     

Poetic transformations of yolmo ‘sadness’

One way in which Yolmo Sherpa of Helambu, Nepal, come to terms with loss is to sing of it. The paper seeks to show how one Yolmo song of sadness works to express, evoke and assuage sentiments of funerary grief. Yet to best explore the transformative poetics of the song, we must trace its semantic links to other songs and situations, and so develop a contextual understanding of the experiential contours of Yolmo sadness, the local ethos of emotional avoidance and restraint, and the sociopolitical nature of emotional distress in the Helambu valley. The findings of this analysis lead the author to argue, in contrast with recent ethnographies which treat discourses on emotions as rhetorical strategies rather than as reflections of personal or communal experience, that we need an integrative approach which focuses on the relationship between language and experience, politics and felt emotion.     

Implementation of a ‘standard’ LIMS

This article is an extract of a presentation given at the LIMS conference in the Hague June 4, 1997 [S.M. Fransen et al., Presentation given at the conference LIMS 97, June 4 1997 in the Hague.]. It describes the project steps for the LIMS project in the Danish company Novo Nordisk, with focus on vendor evaluation, validation and implementation of a ‘Standard’ LIMS software. The main project steps are described and experiences gained are discussed.     

Microbiology of the fermentation of ‘iru pete’ and ‘iru woro’ obtained from local producers

Summary The microorganisms responsible for the fermentation of iru, obtained from local producers, were isolated and identified. Only bacteria were involved in the fermentation; the species areMicrococcus luteus, Staphylococcus aureus, Bacillus subtilis, Lactobacillus plantarum, Citrobacter freundii, Lactobacillus salivarius andStreptococcus pyogenes. Microbial population, temperature, pH and moisture content increased with increase in period of fermentation. A progressive in titratable acidity accompanied the fermentation.

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Resumen Se aislaron e identificaron los microorganismos responsables de la fermentación de iru obtenido de productores locales. En el proceso de fermentación unicamente estaban implicadas bacterias que se identificaron como:Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis, Lactobacillus plantarum, Citrobacter freundii, Lactobacillus salivarius yStreptococcus pyogenes. La población microbiana, la temperatura, el pH y el contenido en humedad se incrementaron al aumentar el periodo de fermentación. Se observó un decremento progresivo del título de acidez a lo largo de la fermentación.

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Résumé Les microorganismes responsables de la fermentation des iru obtenus de producteurs locaux ont été isolés et identifiés. Seules des bactéries sont impliquées dans la fermentation. Les espèces sontMicrococcus luteus, Staphylococcus aureus, Bacillus subtilis, Lactobacillus plantarum, Citrobacter freundii, Lactobacillus salivarius etStreptococcus pyogenes. La population microbienne, la température, le pH et la teneur en eau agumentent avec l'augmentation du temps de fermentation. Une baisse progressive de l'acidité titrable accompagne la fermentation.

     

‘Conservative’ and ‘variable’ clusters of Alu-family DNA repeats in human chromosomes

The distribution of Alu-family DNA repeats (AFRs) in chromosomes of phytohaemagglutinin-stimulated peripheral blood lymphocytes of four normal donors and non-stimulated bone marrow cells of four patients with acute leukemia (ALL and ANLL) was studied by in situ hybridization using DNA of recombinant phage lambda containing multiple inserts of AFR as a probe. Over some chromosome bands (14cen, 16p13, 16cen) from normal donors and from leukemic patients clusters of silver grains were detected. Over other three bands (3q26, 8p11-p12 and 14q24) the clusters were found only in chromosomes from the four acute leukemia patients, and were absent from chromosomes of healthy donors. The results suggest non-random long-range distribution of AFRs in human chromosomes, and somatic variations in the distribution of the repeats.     

‘Hip to be Irish’: ethnicity and Bourdieu's ‘Forms of Capital’

A ‘new Irish’ American ethnicity surfaced in the 1980s – according to a number of scholars and journalists – and is comprised of what some consider to be more ‘authentic’ and ‘traditional’ Irish cultural attributes. Defining authentic and traditional Irishness is complicated, however, by the recent influx and highly visible forms of economic capital and media attention stemming from the Celtic Tiger – the surge of economic prosperity that began in Ireland in the 1990s – and the commercializing of Irish music and dance – such as Riverdance. In this paper, I propose, through my study involving Irish language enthusiasts, that this ‘new’ form of Irish ethnicity is more the result of rather than the reason for this surge in popularity. I use Pierre Bourdieu's ‘Forms of Capital’ in questioning the ‘new Irish’ American ethnicity, arguing instead that a deeply seated, less-visible reservoir of cultural and social capital form the basis for this perceived contemporary ethnicity.     

The ‘division of labour’ model of eye evolution

The ‘division of labour’ model of eye evolution is elaborated here. We propose that the evolution of complex, multicellular animal eyes started from a single, multi-functional cell type that existed in metazoan ancestors. This ancient cell type had at least three functions: light detection via a photoreceptive organelle, light shading by means of pigment granules and steering through locomotor cilia. Located around the circumference of swimming ciliated zooplankton larvae, these ancient cells were able to mediate phototaxis in the absence of a nervous system. This precursor then diversified, by cell-type functional segregation, into sister cell types that specialized in different subfunctions, evolving into separate photoreceptor cells, shading pigment cells (SPCs) or ciliated locomotor cells. Photoreceptor sensory cells and ciliated locomotor cells remained interconnected by newly evolving axons, giving rise to an early axonal circuit. In some evolutionary lines, residual functions prevailed in the specialized cell types that mirror the ancient multi-functionality, for instance, SPCs expressing an opsin as well as possessing rhabdomer-like microvilli, vestigial cilia and an axon. Functional segregation of cell types in eye evolution also explains the emergence of more elaborate photosensory–motor axonal circuits, with interneurons relaying the visual information.     

‘Kumanasamuha’ a new genus of dematiaceae

Summary Kumanasamuha a new genus of Dematiaceae is described, collected growing as saprophyte on unidentified wood, from Pakhal forest, withK. sundara as type.     

Stable propagation of ‘selfish’ genetic elements

Extrachromosomal or chromosomally integrated genetic elements are common among prokaryotic and eukaryotic cells. These elements exhibit a variety of ‘selfish’ strategies to ensure their replication and propagation during the growth of their host cells. To establish long-term persistence, they have to moderate the degree of selfishness so as not to imperil the fitness of their hosts. Earlier genetic and biochemical studies together with more recent cell biological investigations have revealed details of the partitioning mechanisms employed by low copy bacterial plasmids. At least some bacterial chromosomes also appear to rely on similar mechanisms for their own segregation. The 2 μm plasmid ofSaccharomyces cerevisiae and related yeast plasmids provide models for optimized eukaryotic selfish DNA elements. Selfish DNA elements exploit the genetic endowments of their hosts without imposing an undue metabolic burden on them. The partitioning systems of these plasmids appear to make use of a molecular trick by which the plasmids feed into the segregation pathway established for the host chromosomes.     

100 Years of Research on ‘Turbellaria’

Comprehensive research on the ‘Turbellaria’ – or what are known now as the free-living platyhelminths – can mark its beginnings with the first monographic treatises on the group, foremost those by v. Graff, Lang, Wilhelmi and Benham. A major center for turbellarian research grew up around Graff in Graz, Austria, from 1884 through the first quarter of the 20th century; among its members were Böhmig, A. Meixner, J. Meixner, Reisinger and Steinböck. Other contributors to the major research effort in the later years of the 19th and early part of the 20th century were scattered in Germany, Italy, France, Scandinavia, Russia, Japan and the United States and included Hofsten, Steinmann, Wilhelmi, Sabussov, Beauchamp, Bresslau, Bock, Kenk, Ijima and Kaburaki. The attention to detail and accuracy of presentation by these early workers in Graz and elsewhere has made their contributions of long-lasting value. Between 1910 and 1960, modern research whose impact is still widely felt today took form. It includes studies in systematics (Luther, Karling, Hyman, Meixner, Beauchamp, Ijima, Ivanov, Mamkaev, Kenk, Papi, Riedl, Marcus, Ax), development (Bresslau , Reisinger, Steinböck), physiology (Hyman, Westblad), and genetics (Benazzi). Electron microscopy had a profound impact on morphological study starting in the 1970's, and the data it provided led to a new phylogenetic system of the Platyhelminthes as a whole, emphasizing the paraphyletic nature of the ‘Turbellaria’ within it. Systematic biology can be expected to make further advances by combining molecular methods with high-quality traditional taxonomic ones, still essential to this field. Other methodological advances spurring turbellarian research today include immunocytochemistry, molecular biology of nucleic acids, karyology and computational methods. Better understanding of nervous systems and muscle systems should come through studies in functional biology, immunocytochemistry, fluorescence microscopy and molecular biology. Understanding the early evolution of the brain as a control center in the nervous system will be especially interesting. Studies of structural and regulatory proteins as well as the newer microscopical techniques should provide insight into the regulation of cell proliferation, tissue growth, and embryonic development. Ecology and behavior are areas in which research efforts should be intensified. Culturing and the new techniques in molecular biology, microscopy and computational methods now allow the collection of quantitative, experimental data in these fields. Current karyological and biogeographical studies as well as work on the evolution of sex in hermaphrodites are encouraging signs that we might soon better understand many aspects of reproduction and of interactions of free-living platyhelminths in their ecosystems. Turbellarians remain important research subjects for reasons that inspired interest by even the earliest workers, the probability that they are the most primitive living representatives of the Bilateria.