The Distribution of Nine Elements in Shoots of Lupinus albus L. and Lupinus angustifolius L. Compared with that of Silicon as a Measure of Passive Transport

The Ca, Mg, K, Na, P, Fe, Mn, Zn, Cu and opaline-Si contentsof leaves, stems and inflorescences from each order of shootaxis was determined in Lupinus albus L. and Lupinus angustifoliusL. The distribution of Si was used as a base for passive transportin the transpiration stream. All of the elements investigatedwere redistributed among the leaves, stems and inflorescencesof either L. albus or L. angustifolius. Most of the elementsinvestigated were enriched in the inflorescences and depletedin either the leaves or the stems. Sodium was enriched in thestems whereas Ca and Mn were redistributed only in L. albus.The pattern of element redistribution was similar in each ofthe lateral shoot axes except for the youngest. For the elementsenriched in the inflorescences, more than half was suppliedby redistribution. Calcium redistribution was similar to thatfor K, which is regarded as phloem mobile, but a mechanism forCa redistribution is uncertain. Lupinus albus L., Lupinus angustifolius L., mineral transport, leaves, inflorescences, transpiration, silicon, calcium     

Variation in the growth of lupin species and genotypes on alkaline soil

Commercial lupins grow poorly on alkaline and neutral fine-textured soils. Genotypic variation exists among lupins. The present study compared the growth of 13 lupin genotypes, including introduced cultivars and wild types, in an alkaline loamy soil and an acid loamy soil.Plants grown in the alkaline and acid soils did not show obvious symptoms of iron deficiency at any stage. There was however a large variation of shoot fresh weight among genotypes in response to the alkaline soil with L. atlanticus and L. pilosus being more tolerant than L. luteus, L. cosentinii, L. albus and L. angustifolius. Some variation also existed among genotypes of L. angustifolius. In addition, root growth was retarded on the alkaline soil except for L. atlanticus, L. pilosus P20955 and L. albus Kiev mutant. In the alkaline soil, root growth at week 2 correlated well with the shoot fresh weight at week 12. The results suggest that early root elongation may be useful for screening tolerant genotypes for alkaline soils.     

Density Studies on Lupins. II. Components of Seed Yield

Components of seed yield of cv. Ultra (Lupinus albus L.) andcv. Unicrop (L. angustifolius L.) were measured when grown atthree densities. The low density (10 plants m^–2) Unicropyield (34 g seed per plant) was 1.8 times that of Ultra as ithad more branches, pods and seeds per pod. Ultra seeds (310mg per seed) were heavier than Unicrop seeds (180 mg). The branchingpattern of Ultra was less dependent on plant density, henceat 93 plants m^–2 it gave a higher per plant yield (7.4vs 6.4 g) than Unicrop at lower densities (83 plants m^–2).Density had most influence on pod formation and only small effectson seeds per pod and seed weight. Yield components on the main-steminflorescence were influenced less by density than componentson branch inflorescences. Later formed, higher order generationsof inflorescences were most affected by increased inter- andintra-plant competition. Pod numbers on the main-stem were similarfor both species. Pods formed at higher flower nodes in Unicrop,but the lower flower nodes were less fertile than those in Ultra.Node position of flowers had no influence on seed set in main-stemUnicrop pods, but pods from higher nodes in Ultra formed fewerseeds. Seed weights in Unicrop were similar among main-stemnodes but in Ultra seed weights tended to increase at highernodes. Lupinus spp, lupins, seed yield, planting density     

Manganese Nutrition of Lupinus spp. Especially in Relation to Developing Seeds

Patterns of transport and accumulation of manganese were studiedin Lupinus albus L. and Lupinus angustifolius L. in a wide rangeof availability levels in the rooting medium. The recently described‘split seed’ disorder, involving discolouration,splitting, and deformity of seeds, was reproduced in sand cultureusing critically low levels of manganese. The disorder was preventedby maintaining adequate manganese in the medium and its incidencein field and glasshouse was quantitatively related to the managneselevel in seed and fruit phloem sap. The use of phloem sap analysisfor early diagnosis of the disorder is suggested. High levelsof manganese in parent seed is suggested to afford protectionagainst the disorder by improving early vegetative growth ina manganese deficient situation. Direct carry-over of manganesefrom one seed generation to the next was insignificant. Manganese proved to be fully mobile in xylem but only sparinglymobile in phloem from vegetative structures to seed. It wasaccumulated in massive amounts in leaves and fruits when availabilitywas high. Seed manganese content increased 80–100 foldas the level in the rooting medium was increased from 0•1to 500 mg Mn 1^–1. L. albus was superior to L. angustifoliusin accumulating manganese in leaves and pods, and more efficientin translocating the element to its seeds. These differenceswere greatest at low or moderate manganese levels. Xylem intakeby a fruit was small relative to phloem intake when manganeseavailability was low, but became increasingly important as thesupply in the rooting medium was raised.     

Density Studies on Lupins: I. Flower Development

In an August-sown experiment the pattern of flower developmentwas followed for cv. Ultra (Lupinus albus L.) and cv. Unicrop(L. angustifolius L.) grown at low (10 plants m^–2) andhigh (93 and 83 plants m^–2, Ultra and Unicrop respectively)densities. Dry weight increase of flowers on the main-stem inflorescenceand first lateral below the main-stem were compared at differentfloral stages. Maximum flower weight was reached just priorto the open flower stage and remained constant or declined untila pod formed or abscission occurred. The time period betweenmaximum flower weight and pod formation or abscission was upto 10 days. Emergence of the inflorescence was earlier and thefirst flower of Ultra opened 10 days before Unicrop. Developmentof each terminal raceme (inflorescence) was acropetal, withpods having formed on lower flower nodes when terminal flowerswere still quite immature. Laterals forming the next generationof inflorescences grew from axillary leaf buds below an inflorescencewhile it was in full flower. Sources of competition from connectedreproductive and vegetative metabolic sinks are discussed. Lupinus spp., lupins, flower development, planting density     

pH above 6.0 reduces nodulation in Lupinus species

Lupinus angustifolius and Lupinus pilosus differ substantially in root growth in response to high solution pH with L. angustifolius showing much greater sensitivity to pH above 6. This study examined the effect of pH above 6 on nodulation of these two species in buffered solution. Shoot weight and root weight and length in L. pilosus was not significantly affected by pH, whereas the growth of shoots and roots of L. angustifolius was markedly impaired by increasing pH. Total root length, the number of lateral roots, and the length of individual lateral roots were greatly decreased, resulting in decreased uptake of iron and phosphorus. In addition, L. angustifolius had a higher internal requirement for iron than L. pilosus. A solution pH above 6 decreased the number of nodule initials and nodules similarly in both species but decreased nodule mass much more in L. angustifolius. The effect of high pH on nodule formation occurred prior to that on host shoot growth. High pH also decreased nitrogen concentration and content in both species but to a greater extent for L. angustifolius. The results suggested that pH above 6 has a specific effect in the impairment of nodulation in lupins.     

The acquisition of phosphorus byLupinus albus L.

Summary It has been demonstrated by an agar film technique thatL. albus can cause the breakdown of colloids of iron/silicate, iron/phosphate, aluminium/silicate and aluminium phosphate and destabilise suspensions of manganese dioxide, calcium mono-hydrogen phosphate and ferric hydroxide. Dissolution of these compounds was most marked in areas adjacent to proteoid roots (dense clusters of secondary laterals of limited growth which develop on lateral roots) and parts of the tap root. Soil associated with these regions of the root system contained more reductants and chelating agents than the bulk soil. Soil from around the roots ofL. albus exhibited much greater reducing and chelating activity than that associated with the roots of rape and buckwheat.     

Effects of solution pH and bicarbonate on the growth and nodulation of a range of grain legume species

Defining plant adaptation to soil conditions is critical for the successful introduction of grain legume species into farming systems. This nutrient solution study examined the effects of pH (4, 5, 6, 7 and 8) and bicarbonate (5 mM KHCO₃) on the growth and nodulation of 14 grain legume species supplied with N or reliant on N₂-fixation. Species includedPisum sativum L.,Cicer arietinum L.,Lens culinaris Med., and a range ofLupinus, Vicia andLathyrus species. Species differed greatly in response to solution pH. For both N-fertilized and N₂-fixing plants, shoot growth ofL. culinaris was very sensitive to low pH (pH < 7), whereas shoot growth ofLupinus angustifolius L. andLupinus albus L. was sensitive to higher pH (pH ≥ 6). Other species had a broader optimal pH range for growth when supplied with N, but were generally sensitive to low pH (pH < 7 forC. arietinum andVicia sativa L., pH < 6 forP. sativum, Vicia faba L.,Lathyrus sativus L. andLathyrus cicera L., and pH < 5 forVicia benghalensis L. andVicia narbonensis L.) when reliant on N₂-fixation. For these other species, symbiotic N₂-fixation appeared to be more sensitive than host plant growth to low pH. This finding was supported by lower nodule numbers and mass, and lower N concentrations in shoots of sensitive species at low pH relative to higher pH. ForL. culinaris, nodule numbers and mass were relatively unaffected by pH 5–8, N concentrations in shoots were high at low pH and plants developed symptoms relating to H⁺ toxicity at pH as high as 7. These results indicate that host plant growth ofL. culinaris is more sensitive to low pH than theRhizobium symbiosis. ForL. albus andL. angustifolius, both host plant growth and symbiotic N₂-fixation appeared to be equally sensitive to pH ≥ 6.Lupinus pilosus Murr. was more tolerant of high pH than the otherLupinus species. At pH 4, two genotypes ofC. arietinum had better early nodulation than other species.Vicia ervilia L. nodulated poorly at all levels of solution pH, indicating that the commercial Group E inoculum (Rhizobium leguminosurum bv.viceae SU303) may not be effective for this species in solution culture. Addition of bicarbonate decreased shoot growth, nodulation and N concentrations in shoots of most species. Early nodulation (nodule number) ofLathyrus ochrus (L.) DC was not affected by the bicarbonate treatment.     

Anther culture of <Emphasis Type="Italic">Lupinus angustifolius</Emphasis>: callus formation and the development of multicellular and embryo-like structures

Androgenesis is an important technique to generate double haploid plants. Anther and microspore cultures are the methods to induce haploid embryogenesis. For culture initiation, it is necessary to select anthers with the appropriate developmental stage of microspores. For lupins, limited reports about the establishment of initial cultures for androgenesis are available. In this study, different parameters of anther culture of three genotypes of Lupinus angustifolius were investigated. For all genotypes, a considerable correlation was observed between the buds and the anthers, depending on their location in the inflorescences. Buds from the central segment of inflorescences had yellowish green anthers that contained the maximum number of microspores at uninucleate stage. Cytological investigation shows that the anthers containing these microspores were the most responsive to induction. Two types of developmental pathways were observed for microspores. In case of cold pre-treated and untreated inflorescences, microspores developed into multicellular and embryo-like structures, respectively. Effects of different factors showed significant differences among: genotypes, pre-treatment, growth regulators (GRs) and genotypes × GRs interaction. Among three genotypes, Emir showed the highest number of multicellular and embryo-like structures on MS medium + 2.0 mg/l 2,4 D + 0.5 mg/l Kinetin (Kin). For all genotypes, anthers produced calli on MS medium containing 2.0 mg/l 2,4 D + 0.5 mg/l Kin. These calli continued their growth on regeneration medium (MS + 2.0 mg/l BA + 0.5 mg/l NAA) and produced roots. Taken together, these results provide a good basis for further research towards the development of haploid plants for L. angustifolius.     

Comparison of the response to phosphorus deficiency in two lupin species,Lupinus albus and L. angustifolius,with contrasting root morphology

White lupin (Lupinus albus) produces cluster roots, an adaptation to low soil phosphorus (P). Cluster roots exude large levels of P‐solubilizing compounds such as citrate and malate. In contrast, narrow leaf lupin (L. angustifolius) is closely related to L. albus, but does not produce cluster roots. To examine the different strategies for P acquisition, we compared the growth, biomass allocation, respiratory properties and construction cost between L. albus and L. angustifolius under P‐deficient conditions. Both Lupinus species were grown in hydroponic culture with 1 or 100 μM P. Under the P‐deficient regime, L. albus produced cluster roots with little change in biomass allocation, while L. angustifolius significantly increased biomass allocation to roots. The rate of cyanide‐resistant SHAM (salicylhydroxamic acid)‐sensitive respiration was high in cluster roots and very low in roots of L. angustifolius. These results suggest a low alternative oxidase (AOX) activity in L. angustifolius roots, and thus, ATP would be produced efficiently in L. angustifolius roots. The construction cost was highest in cluster roots and lowest in L. angustifolius roots. This study shows that under P deficiency, L. albus produces high‐cost cluster roots to increase the P availability, while L. angustifolius produces large quantities of low‐cost roots to enhance P uptake.     

Cluster-root production and organic anion exudation in a group of old-world lupins and a new-world lupin

We examined the capacity of several Old-World lupin species (Lupinus luteus L., L. hispanicus Boiss. et Reuter and L. angustifolius L.) and one species of a New-World lupin (L. mutabilis Sweet) to form cluster roots under a range of conditions in solution culture. The effect of the synthetic auxin, IBA (indole-3-butyric acid), on cluster-root development in L. luteus and L. albus L. provided with an adequate phosphorus (P) supply was also investigated. In addition, the effect of a high nitrate-N (NO₃-N) supply on the efflux of citrate and malate from roots of L. angustifolius was examined to determine if specific regions of the root system exuded these organic anions. When P-deficient, L. hispanicus, L. luteus and L. mutabilis formed cluster roots that secreted organic anions. Citrate was generally the dominant organic anion exuded, although succinate was also exuded in large quantities from L. luteus. Citrate efflux by L. hispanicus and L. luteus was at least comparable to that reported for P-deficient L. albus[up to 1.092 nmol g^–1 fresh weight (FW) s^–1], but was over an order of magnitude lower in L. mutabilis (0.036 nmol g^–1 FW s^–1). Citrate and malate were not detected in significant amounts from either the lateral roots or the root tips of any species grown under P-sufficient or -deficient conditions. Citrate efflux from cluster roots of L. luteus showed a diurnal pattern, similar to that reported for L. albus, with maximum efflux during the day, and declining to a minimum before dawn. IBA added to the nutrient solution induced cluster-root formation on both L. albus and L. luteus at concentrations of P that would normally suppress the production of these roots. However, the IBA-induced cluster roots did not exude significant amounts of citrate. Although L. angustifolius did not produce cluster roots when P-deficient, it produced cluster-like root structures that exuded citrate (0.053 nmol g^–1 FW s^–1) when grown at a high nitrate-N (NO₃-N) supply. L. angustifolius did not exude significant citrate or malate from lateral roots or root tips when grown at either high or low NO₃-N supply. Our findings for L. hispanicus and L. luteus are the first reports of cluster-root formation in response to P deficiency for these Old-World species, and for L. mutabilis, it is the first report of cluster roots for a New-World lupin species. These reports indicate that evolutionary and biogeographical aspects of cluster-root formation in the genus Lupinus need to be revised. Furthermore, investigation is warranted to determine the capacity of species of the large group of New-World lupins to form cluster roots in soils of their native habitats.     

Sensitivity of different Lupinus species to calcium under a low phosphorus supply

To study mechanism underpinning the calcifuge habit of some Lupinus species, especially under low‐phosphorus (P) conditions, Lupinus species that were likely to respond differently to calcium (Ca) availability were assembled, and the sensitivity to Ca under a low‐P supply was assessed. Seven Lupinus species (9 genotypes, L. albus L. cv Kiev, L. albus L. P26766, L. angustifolius L. cv Mandelup, L. angustifolius L. P26723, L. luteus L. cv Pootalong, L. hispanicus ssp. bicolor Boiss. and Reut. P22999, L. pilosus Murr. P27440, L. cosentinii Guss. P27225, and L. atlanticus Gladst. P27219) were grown hydroponically at 10 or 6000 μM Ca. Leaf symptoms, gas exchange and biomass were recorded; leaf and root nutrient concentrations were analysed, and the leaf cell types in which Ca and P accumulated were determined using elemental X‐ray microanalyses. Calcium toxicity was demonstrated for L. angustifolius P26723, L. hispanicus ssp. bicolor. P22999, and L. cosentinii P27225, whereas the other species were tolerant of a high Ca supply under low‐P conditions. In addition, genotypic differences in Ca toxicity were found within L. angustifolius. Most Ca accumulated in the mesophyll cells in all species, whereas most P was located in epidermal cells.     

Lupinus species differ in their requirements for iron

The effect of iron supply on the growth and nodulation ofLupinus angustifolius L. (Gungurru),Lupinus luteus L. (R-1171) andLupinus pilosus Murr. (P20957) was studied in acid solutions. Plants of the three species were grown together in the same solution and inoculated withBradyrhizobium (Lupinus) WU 425. Plants were then grown with or without applied NH₄NO₃. The lupin species differed greatly in their sensitivity to low iron concentrations in solution withL. pilosus being most tolerant andL. luteus most sensitive.L. pilosus had the highest iron concentration in tissues and had a higher ratio of iron concentration in the youngest fully expanded leaf blades (YEB) to that in roots than the other two species.L. luteus had higher iron concentrations in roots but lower iron concentration in YEB and shoots than didL. angustifolius. The requirements of internal iron for the maximal chlorophyll synthesis in YEB were 65 μg g^-1 forL. angustifolius andL. luteus, and 52 μg g^-1 forL. pilosus. In contrast to effects on growth, the three species had similar external iron requirements for nodule formation in roots and for maximal nitrogen concentrations in shoots. The results indicate that iron tolerant lupin species require lower internal and external iron supply and have a greater ability than sensitive species to translocate iron from roots to shoots.     

The alkaloid content of sweet lupin seed used in feeding trials on pigs and rats

The alkaloid content of the seeds of several new cultivars of “sweet” lupins was determined. The alkaloid contents ranged from < 0.01% for L. angustifolius cv. Uniwhite to 0.09% for L. albus cv. Neuland (a). The major alkaloid component (70%) of Neuland (a) was identified as dl-lupanine. This was the sample of L. albus that markedly suppressed feed intake and growth of young pigs; alcohol extraction reduced the alkaloid content to 0.02% and the extracted lupin-seed meal supported growth and food consumption equivalent to Uniwhite.Rats given a diet containing 50% L. albus cv. Neuland (a) grew normally over 13 weeks. Rats given Neuland (a) as the sole source of protein at 10% of the diet, had a protein efficiency ratio of 0.13. Supplementation of the diet with methionine increased the p.e.r. to 2.45. The addition of increasing amounts of lupin alkaloid, up to maximum content of 0.15% of the diet, had no effect on p.e.r. The p.e.r. obtained from diets containing ethanol-extracted Neuland (a), with and without methionine, were 0.94 and 2.61 respectively, as compared to 0.36 and 2.60 for the unextracted Neuland (a) diets, suggesting that lupin alkaloids can reduce protein quality in methionine deficient diets.The conclusion is that, in marked contrast to pigs, rats are more resistant to dietary lupin alkaloids.     

Effects of pH and bicarbonate on the nutrient status and growth of three Lupinus species

Aims

High pH, and high bicarbonate (HCO₃⁻) and calcium (Ca) availability characterise calcareous soils. High [Ca] only partially explains why some Lupinus species are calcifuge, so we explored high [HCO₃⁻] and high pH.

Methods

We grew six Lupinus genotypes in hydroponics with pH 5, 6.5 and 8^a (adjusted by KOH), and 8^b (adjusted by KHCO₃). Leaf symptoms and areas, root appearance and biomass were recorded; whole leaf and root nutrient concentrations, and leaf cellular phosphorus (P), Ca and potassium (K) concentrations were determined using elemental X-ray microanalysis.

Results

Chlorosis was observed in young leaves at high pH for L. angustifolius and L. cosentinii, and P deficiency at high pH for all genotypes. High pH decreased iron (Fe) and zinc (Zn) uptake in all genotypes. It also decreased lateral root growth, the uptake of P, K, Ca, and manganese (Mn) by all sensitive species; and translocation of P, Fe, Zn, Mn, and Ca to leaves in most sensitive species. However, leaf [Ca], leaf [K], [K] within each measured cell type, and translocation of K and Ca to leaves of L. pilosus and L. cosentinii at pH 8 were greater than at pH 5 and 6.5. Compared with pH 8^a, all L. angustifolius genotypes translocated more P, Fe, Zn, Mn and K from roots to leaves at pH 8^b. High pH did not affect the leaf cell types that accumulated P and Ca, but decreased the leaf cellular [P].

Conclusions

Lupinus angustifolius and L. cosentinii were sensitive to high [HCO₃⁻] and/or high pH; L. pilosus was relatively tolerant. High pH decreased lateral root growth and nutrient uptake, inhibiting growth of sensitive species. High [HCO₃⁻] diminished the negative effect of pH 8 on nutrient translocation to leaves in most L. angustifolius genotypes. This knowledge provides critical insights into the habits of Lupinus species to guide breeding of calcicole plants.

     

Vegetationsbeobachtungen an Lupinen in verschiedenen geographischen Breiten

Zusammenfassung Es wurden die Vegetationsdaten von Anbauversuchen in den Jahren 1934–1939 mitL. luteus, L. angustifolius undL. albus in zwei auf verschiedenen geographischen Breiten ligenden Orten miteinander verglichen (Müncheberg =52°N, Sarona =32°N).Im Durchschnitt der Jahre ergab sich in Sarona beiL. luteus undL. angustifolius eine mehr als 100%ige Verlängerung der vegetativen Phase gegenüber dem Anbau in Müncheberg. Der Unterschied beiL. albus betrug nur 18%.Bei dem Anbau 1934/35 in Sarona kommt für die Erklärung des Unterschiedes fast ausschließlich die unterschiedliche Tageslänge in Frage, während in den übrigen Jahren auch die Temperatur zur Zeit der Keimung eine wichtige Rolle spielt.Für vergleichbares Material beiL. luteus konnte festgestellt werden, daß die Blühverzögerung etwa je zur Hälfte auf die Wirkung der beiden Wachstumsfaktoren zurückzuführen ist.Von den einzelnen Vegetationsphasen wird beiL. luteus undL. angustifolius der Abschnitt Aufgang—Blühbeginn am stärksten beeinflußt, während der Abschnitt Blüte—Reife nur geringe Unterschiede aufweist. BeiL. albus liegen die Verhältnisse eher umgekehrt.BeiL. angustifolius undL. albus konnten stammweise, wahrscheinlich, genetisch bedingte Unterschiede in der Reaktion auf die verschiedenen Umweltseinflüsse festgestellt werden.Zum Schluß werden die aus den Beobachtungen sich ergebenden züchterischen und anbautechnischen Folgerungen besprochen.     

Redistribution of Nutrient Elements from Cotyledons of Two Species of Annual Legumes During Germination and Seedling Growth

The distribution of dry matter and various mineral nutrientsbetween testa and embryo of seeds of Lupinus albus and L. angustifoliusis described It was found that lupin seeds at either end ofa pod contained less dry matter and minerals than seeds in themiddle of the fruit. The transport of dry matter, N, P, K, S,Ca, Mg, Na, Fe, Zn, Mn and Cu from cotyledons of parent seedsof both species to the seedling axis was measured from germinationto the time of cotyledon death. N, P, K and S were retrievedfrom cotyledons with over 90 per cent efficiency, dry matter,Mg, Na, Fe, Zn, Mn and with 59–90 per cent efficiency,and Ca with 26–31 per cent efficiency. There was littlechange in the efficiency of nutrient retrieval from cotyledonswhen seedlings were grown in different culture media. Both speciesshowed a linear relationship between the loss of each elementand dry matter throughout the experiment, and a similar proportioningbetween root and shoot of the amount of a specific nutrientmobilized from cotyledons of parent seeds. Lupinus albus L., Lupinus angustifolius L., lupin, transport, of dry matter and mineral nutrients     

Two new species of Ophiocaryon (Sabiaceae) from South America

Ophiocaryon barnebyanum from the Serra da Neblina (Venezuelan-Brazilian border) andO. neillii from southern Ecuador are described and illustrated, and their morphological relationships with allied spcies are discussed.Ophiocaryon barnebyanum is morphologically similar toO. duckei, but it differs by its larger leaves, leaflets, petioles, petiolules, and inflorescences, the higher number of secondary veins, and the ciliate sepals.Ophiocaryon neillii is a small tree related toO. klugii but differs by its smaller leaflets, petiolules and inflorescences, fewer secondary veins, lanceolate-acuminate sepals, and orbicular staminodes. An updated key to the species ofOphiocaryon is provided.     

Screening rough-seeded lupins (Lupinus pilosus Murr. and Lupinus atlanticus Glads.) for tolerance to calcareous soils

Soil- and solution-based screening methods were used to identify interspecific and intraspecific variation in lupins for tolerance to calcareous soils. Plants were grown for 21 days in a calcareous soil (pH 8.2; 50% CaCO₃; moisture content 90% of field capacity) for soil-based screening and in nutrient solution containing 15 mM KHCO₃ for solution-based screening. Chlorosis as an indicator of tolerance was recorded. Lupinus pilosus Murr. had the most tolerant genotypes and had the greatest range of intraspecific variation. Most genotypes of Lupinus atlanticus Glads. and Lupinus angustifolius L. were moderately intolerant, although two genotypes of L. atlanticus appeared to be tolerant. Lupinus albus L. had moderately tolerant to moderately intolerant genotypes, whilst the single genotypes of Lupinus cosentinii Guss. and Lupinus digitatus Forsk. appeared tolerant. In a field study six genotypes of L. pilosus identified in the soil-based screening as differing in their tolerance to the calcareous soil were grown on comparable calcareous (pH 8.3; topsoil 3% CaCO₃, subsoil 13% CaCO₃) and non-calcareous (pH 7.3) soils within a paddock. Chlorosis and nutrient concentrations in the youngest leaves were measured 53 days after sowing, whilst grain yield was estimated at harvest. Despite the soil containing a much lower CaCO₃ content than used in the screening method, the field study confirmed that moderately intolerant to intolerant genotypes had lower relative grain yields than more tolerant genotypes. Chlorosis rankings of the genotypes were correlated between field and the screening studies. It is suggested that the incorporation of genes conferring tolerance to calcareous soils into high yielding, agronomically suitable genotypes of L. pilosus should be an important objective in a lupin breeding program for calcareous soils.     

Electrophoretic patterns of seed albumins in the Old-WorldLupinus species (Fabaceae): Variation in the 2S albumin class

PAGE and SDS-PAGE electrophoretic analysis of 31 accessions of 10 Old-WorldLupinus species, 5 smooth-seeded and 5 rough-seeded, covered total seed albumins and 2S albumins isolated by a solid-phase extraction. PAGE albumin patterns showed a distinct difference between the smooth- and rough-seeded lupins. SDS-PAGE analysis of seed albumins revealed interspecific differences, mainly due to the 2S albumins. The differences were especially marked in the smooth-seeded species. In the rough-seeded lupins the following subgroups were distinguished: (1)L. atlanticus, (2)L. cosentinii andL. digitatus, (3)L. palaestinus andL. pilosus. Evidence was provided that the 2S albumin class contains conglutin , so far classified as a globulin. The results are discussed with reference to taxonomic relationships of the Old-World lupins and characterization of the lupin seed albumin fraction.