共查询到20条相似文献,搜索用时 15 毫秒
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Dr. M. N. Moore 《Cell and tissue research》1976,175(3):279-287
Latent beta-glucuronidase and glucosaminidase activities have been demonstrated in small cytoplasmic particles, which may possibly be primary lysosomes, as well as some larger granules of the digestive cells of the common mussel. Latency was indicated by increased staining of these structures following incubation in buffer at pH 4.5 at 37 degrees C. The exposure of mussels to temperatures of 25-28 degrees C over a period of four days induced a significant decrease in the latency of lysosomal glucosaminidase. Thermal death produced labilization of lysosomes although selective release of hydrolase activity was indicated by the differential latency of glucosaminidase and glucuronidase. The injection of hydrocortisone induced a significant increase in latency in stressed animals, indicating that the stress response involved changes in structure and function of membranes. 相似文献
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Hydrolytic enzymes associated with the granular haemocytes of the marine mussel Mytilus edulis 总被引:8,自引:0,他引:8
R. K. Pipe 《The Histochemical journal》1990,22(11):595-603
Summary The ultrastructural localization of a range of hydrolytic enzymes has been investigated in the granular haemocytes of the marine musselMytilus edulis. Arylsulphatase activity and immunocytochemical localization of -glucuronidase and elastase were demonstrated within the large granules of the haemocytes. Lysozyme and cathepsin B were both localized within all sizes of granule, however, at high dilutions the primary antibody against lysozyme was also restricted to the large granules. The labelling density for cathepsin B antibody tended to be very low. Antibodies for cathepsin G showed a clear, discrete labelling which was restricted to the granules of haemocytes containing small granules. The fact that antibodies raised against human proteinases recognize invertebrate enzymes suggests that there must be a certain degree of structural similarity between the human proteinases and the enzymes present in the mussel haemocytes indicating either convergence or conservation of the enzyme molecules. The presence of a range of hydrolytic enzymes including proteinases, glycosidases and sulphatases within the large granules shows that these granules are a form of lysosome. The reduction in activity of lysosomal enzymes in haemocytes following adhesion to glass is evidence for release of the enzymes from the granules (degranulation). The possibility of a serine protease being specifically associated with the small granules and its role as a cytolysin are discussed. 相似文献
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Allozyme variation in a population of the common mussel Mytilus edulis in Mumbles, South Wales, has been studied by starch gel electrophoresis. On the basis of data obtained for 34 loci, we estimate the proportion of loci polymorphic to be 30%. Using only the 29 loci for which individual genotypes can be accurately typed, the average heterozygosity is estimated to be 9.5 +/- 3.6%. The calculated expected average heterozygosity based on Hardy-Weinberg expectations is identical with the observed value. Allele frequency data at six polymorphic loci are given for several other British populations. There is no significant geographic heterogeneity. The results are discussed in relation to genetic adaptive strategies and are shown to be inconsistent with the predictions of the neutral hypothesis. 相似文献
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The lysosomal localization of sphingolipid hydrolases 总被引:4,自引:0,他引:4
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B Kurelec 《Biochemical and biophysical research communications》1985,127(3):773-778
Microsomes from the marine mussel Mytilus edulis possess the enzyme activity that selectively metabolizes primary aromatic amines and not polycyclic aromatic hydrocarbons. This activity is NADPH-dependent and has a pH optimum at 8.4. By these characteristics this enzyme is identical with the purified pig liver FAD-containing monooxygenase (EC 1.14.13.8, dimethylaniline monooxygenase). The exposure of mussels to Diesel-2 oil does not induce the enzyme activity. These results are discussed in terms of possible ecological and environmental significance. 相似文献
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《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1999,122(1):131-137
Mussels (Mytilus edulis) were exposed to trimethyl(carboxymethyl)arsonium bromide (arsenobetaine, C-1 betaine), trimethyl(2-carboxyethyl)arsonium bromide (C-2 betaine), or trimethyl(3-carboxypropyl)arsonium bromide (C-3 betaine). Arsenic was accumulated by the mussels in all cases but the efficiency of uptake decreased with the number of methylene units in the carboxyalkyl group. Arsenobetaine (C-1 betaine) was the most readily accumulated, followed by the C-2 betaine (70% as efficient as arsenobetaine) and the C-3 betaine (∼7%). Chromatographic analysis (HPLC-ICPMS) of extracts of the mussels demonstrated that the arsenic compounds were accumulated uncahanged. A 46-day depuration period which followed exposure did not significantly reduce the arsenic concentration in any of the three groups. Comparison with previous data on accumulation of arsenic compounds by M. edulis indicates that uptake may be influenced by the presence of a quaternary arsonium group and the zwitterionic nature of the arsenic-betaines. 相似文献
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Circulating leucocytes of the mussel, Mytilus edulis, were studied by electron microscopy. Based on morphological criteria, the leucocytes were classified as agranular and granular leucocytes, dependent upon the presence or absence of specific granules in their cytoplasm. Furthermore, the existing literature is being critically revised, and it is suggested that agranular and granular leucocytes might belong to the same cell line. 相似文献
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Arylsulphatase activity associated with phenanthrene induced digestive cell deletion in the marine mussel Mytilus edulis 总被引:3,自引:0,他引:3
Summary The acid hydrolase arylsulphatase has been localized at the ultrastructural level in digestive cells of the marine musselMytilus edulis for control and phenanthrene-treated (200µg/l) animals. In untreated mussels the activity was generally restricted to the lysosomal—vacuolar system and the Golgi apparatus. It was associated with all types of vesicle, although not all individual vesicles were reactive. In heterolysosomes which were filled with precipitate the reaction product was most densely associated with the limiting membranes. Lipid inclusions commonly occurred in the digestive cells; these sometimes showed limited reaction for enzyme activity. The striking difference between normal and phenanthrene-treated samples was the presence in all treated animals of reaction product in the inter-cellular spaces and varying degrees of cytoplasmic activity in a number of digestive cells. This is interpreted as a sign of impending cell deletion. Sections for morphological examination showed evidence of increased digestive cell deletion in phenanthrene-treated mussels. The process results in release of membrane-bound bodies into the tubule lumen. 相似文献
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Mytilus edulis attaches to the substratum by means of a proteinaceous byssus complex. This consists of three portions: a root, embedded in the pedal tissues, a stem, continuous with the root but external to the body and a number of byssus threads attached proximally to the stem and distally to the substratum via adhesive discs. Byssus strength varies seasonally on the shore, in response to changes in wave action (Price, in press). As a decline in byssal attachment strength implies a decline in strength of the constituent threads, a study was undertaken to establish the extent to which byssus thread strength is determined by age. The ultimate tensile stress, ultimate tensile strain and Young's Modulus were measured in threads of known age and length and a stepped regression performed on the results. It was found that age and length correlate significantly with tensile stress and Young's Modulus. Length is a less important influence than age on tensile stress but has a greater effect than age on Young's Modulus. Tensile strain is independent of both length and age. 相似文献
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《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1996,113(2):221-229
Hemolymph of M. edulis is rich in phagocytic hemocytes. Hemocytes contain numerous lysosomes which, in turn, contain various hydrolytic enzymes. Phagocytic activity of M. edulis hemocytes is thought to be associated with NAD(P)H-oxidase activity of the plasma membrane. The laser dye, dihydrorhodamine 123 (DHR), was used for cytochemical and biochemical detection of the generation of reactive oxygen species (ROS) by isolated M. edulis hemocytes. Hemocytes readily take up DHR from the suspension medium and selectively concentrate it in the lysosomes, wherein DHR is oxidized to fluorescent rhodamine 123. Concomitant uptake of DHR with Superoxide dismutase or the spin-trap, tert-phenylbutyl nitrone, but not catalase markedly reduced fluorescence in the lysosomes implicating Superoxide anion (O2−) but not hydrogen peroxide (H2O2) in DHR oxidation. Uptake of the anthraquinone, purpurin, and FeEDTA with DHR greatly amplified fluorescence within the lysosomes. These data are consistent with uptake of xenobiotics by hemocytes and their concentration in lysosomes wherein, ROS are generated in response to their accumulation. The rate of DHR oxidation by hemocytes was not stimulated by zymosan, a known stimulator of the oxidative burst. In vitro studies using the xanthine oxidase/hypoxanthine reaction to generate O2− and selective inhibitors of ROS production indicated that DHR is oxidized by O2− and H2O2 but not by · OH and that iron can participate in the reaction. Incubating isolated hemocytes promoted low-level, SOD-sensitive, FeEDTA-stimulated production of ethylene from α-keto-γ-methiolbutyric acid, indicating the in situ formation of · OH via production of O2−. The above suggest that enhanced production of ROS in M. edulis hemocytes by xenobiotic accumulation within the lysosomal compartment should be considered in the toxic sequelae of exposure of marine molluscs to chemical pollutants. 相似文献
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Proteomic analysis on sperm has been restricted to only a few model organisms. We present here a 2DE PAGE proteome map of sperm cells from a nonmodel organism, the marine mussel Mytilus edulis, a free-spawning marine invertebrate with external fertilization. Ninety-six protein spots showing high expression were selected and of these 77 were successfully identified by nESI-MS analysis. Many of the identifications are relevant to sperm cell physiology and mtDNA functioning. The results and proteomics approach used are discussed in relation to their potential for advancing understanding of the unusual system of mtDNA inheritance described in Mytilus spp., and for the testing of evolutionary hypotheses pertaining to the role of fertilization in the speciation process. 相似文献
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