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MEDENWALD H HABERLAND GL 《Hoppe-Seyler's Zeitschrift für physiologische Chemie》1957,306(4-6):229-234
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Metabolism of thymine (methyl-C14 or -2-C14) by rat liver in vitro 总被引:10,自引:0,他引:10
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During feeding experiments with [omega-14C]oleic acid and [omega-14c]nervonic acid to adult rats, 14C-labelled C26, C28 and C30 fatty acids were recovered from the intestinal mucosa, liver, plasma, kidney and stools. The structures of these fatty acids were determined by g.l.c., radio-g.l.c. and mass spectrometry. The Schmidt and Ginger degradation methods indicated that most of the 14C found in these extra-long fatty acids remained in the omega position. These radioactive extra-long fatty acids were found mainly in the polar lipids of rats killed 3 or 15 h after being fed on labelled oleic acid or nervonic acid. Rats killed 63 h later yielded only traces of these extra-long fatty acids. When the rats were given antibiotics or received the same radioactive fatty acids by intravenous injection, the labelled extra-long fatty acids could not be detected in any of the tissues. We conclude that they were probably synthesized by elongation of oleic acid and nervonic acid by intestinal micro-organisms (probably yeasts) and then absorbed by the intestinal mucosa. 相似文献
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《Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism》1981,663(2):575-577
14C-labeled eicosapentaenoic acid (n-3) was converted by mouse mastocytoma cells to 5-hydroxy-6-S-glutathionyl-7,9,11,14,17-eicosapentaenoic acid (leukotriene C5). The identification was based on comparisons with previously characterized unlabeled material by high-performance liquid chromatography, ultraviolet spectroscopy, and conversion by γ-glutamyl transpeptidase to leukotriene D5. 相似文献
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