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1.
Gregory F. Payne Nadine N. Payne Michael L. Shuler Masanori Asada 《Biotechnology letters》1988,10(3):187-192
Summary A significant fraction (10–40%) of the indole alkaloids produced byCatharanthus
roseus was observed to be secreted into the medium. When a neutral polymeric resin, known to adsorb these alkaloids, was added to the cultivation medium, the accumulation of total indole alkaloids and the specific alkaloids, ajmalicine and serpentine were stimulated. Sorbent addition was also observed to result in increased ratios of ajmalicine to serpentine, which suggests the potential of using in situ adsorption to direct metabolism toward a specific product or intermediate within a given pathway. 相似文献
2.
A. J. Parr A. C. J. Peerless J. D. Hamill N. J. Walton R. J. Robins M. J. C. Rhodes 《Plant cell reports》1988,7(5):309-312
Transformed roots of Catharanthus roseus were obtained following infection of detached leaves with Agrobacterium rhizogenes. Roots would not grow in full strength Gamborg's B5 medium but would grow satisfactorily if the medium was diluted to one half strength. Little alkaloid appeared in the growth medium but root tissue contained a high level and wide variety of alkaloids. Ajmalicine, serpentine, vindolinine and catharanthine were prominent components. Vinblastine could also be detected by a combination of HPLC and radioimmunoassay, though at a level of only 0.05g/g dry weight.Abbreviations B5
Gamborg's B5 nutrient salts
- LC/MS
combined liquid chromatography/mass spectrometry
- FW
fresh weight
- Kb
kilobase 相似文献
3.
Direct somatic embryogenesis on immature zygotic embryos in vitro has been confirmed for Trifolium pratense and extended to T. resupinatum and T. subterraneum. For all species direct embryo cloning can be achieved on an appropriate basal medium supplemented with 1gl–1 yeast extract and 0.05 mgl–1 BAP. Basal medium/sucrose formulation, level of yeast extract and level of BAP affected the nature of in vitro responses. In particular, for T. pratense and T. subterraneum lowering of the yeast extract level suppressed embryoid initiation, and raising of the BAP level stimulated formation of nodular morphogenic callus. For T. resupinatum alteration of the basal medium/sucrose formulation changed the tissue site of embryoid initiation from hypocotyl to cotyledons or both. Control of embryoid initiation is briefly discussed.Abbreviation BAP
6-benzylaminopurine 相似文献
4.
Stem and petiole explants, obtained from mature trees, ofAlbizzia lebbeck,Cassia fistula andC.siamea callused and differentiated shoot-buds and later shoots on B5 medium supplemented with either 0.5 mg/l IAA + 1 mg/l BAP or BM + 2 mg/l NAA + 0.5 mg/l BAP. The stem explants were more responsive than the petiole explants. InA.lebbeck, the IAA substituted medium favoured differentiation from both types of explants. However, inC.fistula, the type of explants rather than the medium composition had an overriding influence on shoot differentiation since those from petiole hardly responded in either medium. It has been possible to obtain plantlets from bothA.lebbeck andC.fistula under conditions conducive to rooting. Plantlets ofA.lebbeck have also been successfully transferred to the field. 相似文献
5.
Plant regeneration from callus cultures of Durum and emmer wheat 总被引:1,自引:0,他引:1
Callus cultures were initiated from isolated mature embryos of Triticum turgidum L. Thell ssps durum and dicoccum on a basal medium supplemented with 2,4-D, 2,4,5-Cl3POP or 2,4-D+CM. Shoot bud regeneration was observed on 2,4,5-Cl3POP medium. In both the cultivars of durum, further development of shoot buds occurred on transfer of tissues to basal medium whereas in dicoccum basal medium supplemented with coconut milk or coconut milk with NAA (0.2 mg/l) was necessary. The regenerated shoot buds were induced to root on basal medium supplemented with NAA. The in vitro obtained plants were transferred to soil and successfully grown to maturity. Chlorophyll variants were observed among the regenerated plants of dicoccum.Abbreviations BA
benzyladenine
- CM
coconut milk
- 2,4-D
2,4-dichlorophenoxyacetic acid
- 2,iP
6---dimethylallylamine purine
- IAA
indoleacetic acid
- NAA
-naphthalene acetic acid
- Kn
kinetin
- 2,4,5-Cl3POP
2,4,5-trichlorophenoxypropionic acid
- MS
modified Murashige and Skoog's medium
- RH
relative humidity
- Z
zeatin 相似文献
6.
Because most interspecific Medicago embryos abort before they can be excised and cultured, our objective was to grow young pods in vitro. Various media were used to grow three-day-old pods of annuals [diploids, M. blancheana Boiss., M. disciformis DC., tetraploid M. scutellata (L.) Mill.] and perennials (diploid M. falcata L., tetraploid M. sativa L.).Few pods of perennial species grew to maturity on media containing modified Hoagland's plus 1% glucose or sucrose with or without 5% potato extract. Increasing sucrose to 6% increased the percentage of M. sativa pods that produced mature seeds. On DM (differentiation medium), the best medium, the percentage of pods producing viable seeds was: M. blancheana (82), M. disciformis (81), M. scutellata (48), M. sativa (63), M. falcata (15). DM plus 1 ppm indoleacetic or gibberellic acid did not enhance seed production.Abbreviations DM
Differentiation medium
- DMI
DM plus 1 ppm indoleacetic acid
- DMG
DM plus 1 ppm gibberellic acid 相似文献
7.
P. Morris 《Plant cell reports》1986,5(6):427-429
A three year old, alkaloid producing cell line of Catharanthus roseus, maintained at 25°C, was grown on 2% sucrose at various temperatures from 10° to 45°C. Growth rates were maximal at 35°C but declined rapidly above 35°C and below 25°C. Maximum serpentine yields reached a peak at between 20°C and 25°C and fell sharply above and below these temperatures, while ajmalicine showed a sharp peak of accumulation at 20°C. The variable serpentine/ajmalicine ratio at different growth temperatures suggests that lower temperatures may favour ajmalicine accumulation. Both the growth rate and the rate of alkaloid accumulation at 25°C were therefore sensitive to small changes in average culture temperature. 相似文献
8.
Adina Breiman 《Plant cell reports》1985,4(2):70-73
Callus cultures were initiated from isolated immature embryos of Hordeum spontaneum and Hordeum bulbosum on MS or B5 basal medium supplemented with 2 mg/1 2,4-D. Shoot regeneration occurred on transfer of tissue to media containing 1 mg/1 IAA and 1 mg/1 zeatin. The regenerated shoot buds were rooted on basal medium without hormones. The in vitro regenerated plants were transferred to soil and were grown to fertile mature plants. A low percentage of albino plants was observed among the regenerated plants. No major differences were detected between the two species in respect to their potency to form callus or to the regeneration capacity. The regeneration capacity of calli decreased gradually and ended after 6 months in culture.Abbreviations IAA
indole-3-acetic acid
- 2,4-D
2,4-dichlorophenoxy-acetic acid
- MS
Murashige and Skoog medium 相似文献
9.
Shoot-tip explants of Rheum emodi Wall. (Polygonaceae) gave rise to multiple shoots when cultured on a Murashige and Skoog (1962) medium (MS) with 2.0 mg/l 6-benzylaminopurine (BAP) and 1.0 mg/l indole-3-butyric acid (IBA). Also, shoot buds developed from leaf explants using MS medium with 2.0 mg/l BAP and 0.25 to 1.0 mg/l indole-3-acetic acid (IAA) or IBA. Roots were induced when the resulting shoots were placed on MS medium with 1.0 mg/l IBA. Both regeneration procedures gave rise to healthy plantlets that were established in soil under glasshouse conditions at 80% frequency after hardening phase of two weeks. Regenerated plants showed a constant chromosome number of 2n=2x=22, same as the parent plant. The use of liquid shake cultures minimized the time and culture medium requirements for propagation. This procedure can be applied for the conservation and utilization of elite clones of R. emodi.Abbreviations BAP
6-benzylaminopurine
- Dd H2O
Double glass distilled water
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- K
Kinetin
- MS
Murashige and Skoog's (1962) medium
- RH
Relative humidity
CIMAP Publication No. 876 相似文献
10.
Habituated and tumorous Catharanthus roseus cells grown in the absence of hormones accumulated indole alkaloids. Total alkaloids and alkaloid pattern were the same when cells were cultured in medium without hormones or in alkaloid production medium with and without indole acetic acid. Treatment of cells with Pythium homogenate as elicitor did not increase total alkaloids or change the pattern of alkaloids produced. When either habituated or tumorous cells were grown in 1B5 medium after Gamborg et al (1968) containing 2,4-dichlorophenoxyacetic acid (2,4-D), their capacity to accumulate alkaloids decreased with time. The levels of tryptophan decarboxylase (TDC) and strictosidine synthase (SS) specific activities were constant throughout growth except when cells were exposed to 2,4-D in 1B5 medium, where enzyme activities declined in step with the decrease in alkaloid accumulation. Neither habituated nor tumorous cell suspension cultures accumulated vindoline, nor could they be induced to produce this alkaloid by any of the given treatments.NRCC No. 27514 相似文献
11.
Stimulation of cell division by membrane-active agents 总被引:8,自引:0,他引:8
Agents that decrease membrane stability (e.g. dimethyl sulfoxide, lysolecithin, sodium oleate, and short-chain alcohols) stimulate multinucleoid, serpentine filaments of Agmenellum quadruplicatum strains SN12 and SN29 to divide into cellular equivalents within approximately one generation time. Agents that increase membrane stability (e.g. long-chain alcohols) antagonize this timulation. Thus, the physical properties of the cell membrane appear to be involved in the regulation of cell division. These observations suggest that the invagination of the cell wall may be regulated by agents that interact with the plasma membrane and with enzymes involved in peptidoglycan synthesis. 相似文献
12.
Addition of the animal ether phospholipid platelet-activating factor, 1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine, (PAF) stimulates medium acidification in cultured soybean (Glycine max L.) cells. The pH of the medium after 8–10 hours is on the average one pH unit lower than in controls. With fusicoccin an average pH difference of 1.7 units is reached. Phospholipids, glycerol, 1-oleyl-2-acetyl-sn-glycerol, 1-0-hexadecyl-sn-glycerol, and triolein at the same concentrations as PAF had no stimulatory effect on medium acidification. The detergents CHAPS and deoxycholate lead to alkalinization of the medium whereas lysophosphatidylcholine (LPC), a detergent with structural similarity to PAF, shows no effect.Abbreviations CHAPS
(3-((3-cholamylopropyl) dimethylamino)-1-propanesulfonate)
- DOC
deoxycholic acid
- FC
fusicoccin
- LPC
lysophosphatidylcholine
- OAG
1-oleyl-2-acetyl-sn-glycerol
- PAF
platelet-activating factor = 1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine
- IAA
indole-3-acetic acid 相似文献
13.
Growth requirements and the establishment of a chemically defined minimal medium inZymomonas
mobilis
Summary A chemically defined minimal medium which fulfils the growth requirements of differentZymomonas
mobilis strains has been established. The kinetics of ethanol production of the strains ATCC 10988, CU1, CP4 and 11163 grown on the minimal medium at different glucose concentrations were measured. All strains produced ethanol at rates similar to those on complete medium. The minimal medium described is suitable to study spontaneous metabolic deficiciencies and regulation of enzyme activities inZ.mobilis. 相似文献
14.
A new material for the solidification of liquid culture media was prepared from plant parenchyma tissues by mechanical subdivision, solute extration and dessication from ethanol. It is suitable for in vitro culture and propagation of callus as well as shoot tip cultures. The following plant materials have been grown by means of the new medium solidifier: shoot cultures of Betula pendula Roth, Gerbera jamesonii H. Bolus ex Hook and Floribunda rose "Triumph", callus tissues of Daucus carota L. and Chenopodium album L. The new solidifying material has special advantages over agar for application in the rooting phase of in vitro propagation.Abbrevations PMS
parenchymatic medium solidifier
- MS
Murashige and Scoog's medium
- IAA
Indole-3-acetic acid
- B
biotin
- K
kinetin
- 2,4-D
2,4-dichlorophenoxyacetic acid
- ch
caseine hydrolysate 相似文献
15.
Summary Embryogenic cultures were established from silver fir (Abies alba Mill.) female megagametliophytes with developing embryos and from excised mature embryos after pollination with Abies cephalonica Lond. or Abies numidica DeLann pollea The frequency of embryogenic callus formation was dependent on genotype, collection time, medium and explants used. The embryogenic callus initiation potential of megagamethophytes with developing embryos in both hybrids was higher in early July and dropped as the zygotic embryos matured. Excised cotyledonary embryos were less suitable for induction of embryogenic cultures. SH medium supplemented with 1mg/l BAP was the most efficient for callus induction and maintenance. Cultures were composed of early somatic embryos with an embryonal mass formed of highly cytoplasmic cells, rich in cell organelles and a suspensor built up by vacuolated, strongly elongated cells. Maturation of embryos was detected with the formation of bipolar structures with shoot and root apices. Nutrition reserves were observed in cells of embryos cultured on DCR medium containing 1 or 10 mg/l ABA. Cotyledon formation, hypocotyl elongation and low frequency germination occured following transfer of the embryos to the same medium without ABA. 相似文献
16.
Summary
Bacillus subtilis CD4, when grown in nutrient broth or minimal medium in presence of xylan, produced extracellular xylanase that hydrolyzed xylan optimally at pH 5. The enzyme was induced by xylan, xylose and glucose. Addition of xylose or glucose in xylan containing medium did not affect enzyme production. The structural gene encoding xylanase was cloned and expressed in E. coli. The recombinant enzyme exhibited similar properties like that of native enzyme including resistance to repression by xylose and glucose. 相似文献
17.
Somatic embryogenesis and plant regeneration in diploid Allium fistulosum × A. cepa F1 hybrid onions
Procedures were developed for disinfestation of non-dormant basal plate tissue excised from field grown basal plate tissue of diploid Allium fistulosum × A. cepa F1 hybrid onions. Contamination levels varied with the season and vegetative development of plant material. Callus initiated from basal plate tissue and immature inflorescences of the F1 hybrids was maintained on a BDS-based medium containing 0.75 mg/l picloram and 2.0 mg/l BA. When this medium was supplemented with vitamins and glycine, and with proline at 2.5 gm/1, somatic embryos began to form. Their development continued on a BDS-based shoot promotion medium containing 0.03 mg/l picloram and 0.32 mg/l 2iP supplemented with vitamins, glycine and proline. Genotypes differed significantly in the numbers of structures regenerated. Plantlets from somatic embryos were rooted into BDS or half-strength BDS medium without growth substances and were successfully transferred to sterilized potting mix in plastic commercial corsage boxes.Abbreviations BA
benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- picloram
4-amino-3,5,6-trichloropicolinic acid
- 2iP
N6-(2-isopentenyl)adenine
- NAA
1-naphthylacetic acid
- BDS
Gamborg's B5 medium modified by Dunstan and Short (1977a) 相似文献
18.
Influence of the inoculum size ofLactobacillus
plantarum on the competition withEnterobacter
cloacae
Summary The size of the inoculum ofLactobacillus
plantarum or its natural density, appears to be of predominant importance in the exclusion ofEnterobacter
cloacae in mixed fermentations, such as ensilage. In a liquid medium, simulating adverse silage conditions, an initial density ofL.
plantarum at least twice that ofE.
cloacae was found necessary in order to obtain a succesful silage. 相似文献
19.
A procedure for the regeneration of fertile plants by organogenesis from tissue cultures of soybeans, Glycine max is described. Seeds were germinated on reduced inorganic salt MS medium containing 5M BA. Cotyledonary nodes were excised and cultured on the same medium. Presence of BA in the medium during seed germination and culture of nodal explants was required for multiple shoot and shoot-bud formation. Histological analyses established the de novo nature of shoot regeneration. Separate reduction of the concentration of inorganic salts or substitution of sucrose for fructose during culture had minimal effects on the regeneration response. Conversely, if the BA was reduced, the inhibition response could not be overcome by increased salt concentration or altered carbon source.Abbreviations BA
benzyladenine
- IAA
indoleacetic acid
- SAS
secondary axillary shoots
- MS
Murashige and Skoog (1962) medium 相似文献