Transient receptor potential vanilloid subfamily 1 expressed in pancreatic islet beta cells modulates insulin secretion in rats

Capsaicin-sensitive afferent neurons including transient receptor potential vanilloid subfamily 1, TRPV1, and neurohormonal peptides participate in the physiological regulation of pancreatic endocrine. However, the direct effect of capsaicin on insulin secretion remains unknown. Our present study showed that TRPV1 is expressed in islet beta cells as well as in neurons in rat pancreas, and also in rat beta cell lines, RIN and INS1. Capsaicin (10(-11)-10(-9) M) dose-dependently increased insulin secretion from RIN cells, and this effect was inhibited by either a TRPV1 inhibitor capsazepine or EDTA. Systemic capsaicin (10 mg/kg, s.c.) increased plasma insulin level 1 h after the treatment. We demonstrated for the first time that TRPV1 is functionally expressed in rat islet beta cells and plays a role in insulin secretion as a calcium channel. This study may account for the influences of capsaicin on the food intake and energy consumption as well as on the pathophysiological regulation of pancreatic endocrine.     

Comparative efficacy of piperine,curcumin and picroliv against Cd immunotoxicity in mice

Cadmium (Cd), a well known environmental carcinogen, is a potent immunotoxicant. In rodents, it is primarily characterized by marked thymic atrophy and splenomegaly. Cadmium induces apoptosis in murine lymphocytes and alters the immune functions. Thus, for the amelioration of its effect, three structurally different bioactive herbal extracts such as piperine—alkaloid, picroliv—glycosides and curcumin—polyphenols were evaluated and their efficacy compared. For ascertaining their immunomodulatory role, various biochemical indices of cell damage such as cytotoxicity, oxidative stress (ROS, GSH), apoptosis (mitochondrial membrane potential, caspase-3 activity, phosphatidylserine externalization, apoptotic DNA) along with lymphocyte phenotyping, blastogenesis and cytokine secretion were assessed in thymic and splenic cell suspensions. Of the three herbals examined, piperine displayed maximum efficacy. All the three doses of piperine (1, 10 and 50 μg/ml) increased cell viability in a dose dependent manner, whereas curcumin and picroliv were also effective, but to a lesser degree. Only the two higher doses exhibited cell viability efficacy. The median doses ie 10 μg/ml, were therefore selected, for comparison of their antioxidant, anti-apoptotic and immune function modulation. Restoration of ROS and GSH was most prominent with piperine. The anti-apoptotic potential was directly proportional to their antioxidant nature. In addition, Cd altered blastogenesis, T and B cell phenotypes and cytokine release were also mitigated best with piperine. The ameliorative potential was in order of piperine > curcumin > picroliv and former could be considered the drug of choice under immunocompromised conditions. An erratum to this article can be found at     

Increased zinc absorption but not secretion in the small intestine of metallothionein-null mice

Metallothionein (MT) has been assigned a role in intestinal Zn absorption and secretion. The influence of MT was investigated in isolated segments of the small intestine from mice lacking the expression of MT I and II genes (MT−/−). To measure Zn absorption, washed 10- to 12-cm segments of the proximal and distal small intestine of MT−/− and control MT+/+ mice were filled with ⁶⁵Zn as ZnSO₄ (10 μg/mL), and the amount of ⁶⁵Zn appearing in the external buffer was measured over 4 h. To measure Zn secretion, the same procedure was followed using everted gut segments. The ⁶⁵Zn absorption from the small intestine was significantly greater in MT−/− mice, but only in the absence of albumin. In the proximal small intestine, the inclusion of 2% albumin in the external buffer significantly increased Zn absorption from 6.8% (no albumin) to 13.2% (with albumin) for MT−/−, and from 4.9% (no albumin) to 14.2% (with albumin) for MT+/+. In the distal segment, the respective values, with and without albumin respectively were 9.5% and 15.1% for MT−/− mice and 4.3% and 16.1% for MT+/+ mice. Regarding ⁶⁵Zn secretion, there was no difference between MT+/+ and MT−/− in either segment. However, the rate of secretion was higher in the proximal small intestine for both genotypes. Although it can be demonstrated that MT limits Zn absorption under controlled conditions in vitro, the ability of albumin to overcome this effect emphasizes the importance of circulating ligands in Zn transport.     

Expression and characteristics of vanilloid receptor 1 in the rabbit submandibular gland

Vanilloid receptor 1 (VR1) is a polymodal receptor originally found in sensory neurons of the central nervous system. Recent evidence indicates that VR1 is also expressed in non-neuronal tissues. We report here endogenous expression of VR1 in rabbit submandibular gland (SMG) and its possible role in regulating saliva secretion based on: (i) the expression of VR1 mRNA and protein detected in SMG; (ii) VR1 was mainly localized in the basolateral membrane of duct cells and the cytoplasm of acinar cells and also in cytoplasm of primary cultured neonatal rabbit SMG cells; (iii) stimulation of neonatal rabbit SMG cells with capsaicin induced a significant increase in intracellular calcium, and capsazepine, a VR1 antagonist, abolished this increase; (iv) infusion of capsaicin via the external carotid artery to isolated SMG increased saliva secretion of the gland. These findings indicated that VR1 was expressed in SMG and appeared to play an important role in regulating saliva secretion.     

红茶对新生幼鼠肠道菌群形成的影响

探讨红茶对新生幼鼠肠道菌群形成的影响,为从微生态角度研究红茶在人体肠道菌群形成过程中的作用机制奠定基础。

利用宏基因组测序技术检测4周龄新生幼鼠（4weeks组,n = 30）、12周龄正常对照组小鼠（control组,n = 15）和12周龄喂饲红茶水实验组小鼠（teadrink组,n = 15）的肠道菌群分布,分析3组样本的菌群差异情况,探求红茶对新生幼鼠肠道菌群形成的影响。

与control组相比,teadrink组小鼠肠道拟杆菌门（t = −7.711,P＜0.001）、拟杆菌科（t = −3.411,P = 0.009）、长尾嗤菌体科（t = −2.515,P = 0.036）、拟杆菌属（t = −2.693,P = 0.027）、邓肯菌属（t = −2.434,P = 0.041）、居海事城球杆菌属（t = −3.327,P = 0.029）、迪博邓肯菌（t = −2.679,P = 0.028）、普通居海事城球杆菌（t = −3.401,P = 0.027）和Duncaniella_sp._C9（t = −3.104,P = 0.035）相对丰度显著增加,厚壁菌门（t = 8.952,P＜0.001）、乳杆菌科（t = 13.102,P＜0.001）、消化链球菌科（t = 3.665,P = 0.021）、爱格菌科（t = 4.481,P = 0.002）、理研菌科（t = 3.626,P = 0.022）、乳杆菌属（t = 5.542,P = 0.004）、黏液乳杆菌属（t = 6.334,P = 0.002）、龙包茨菌属（t = 3.785,P = 0.005）、阿德勒菌属（t = 4.504,P = 0.002）、约氏乳杆菌（t = 4.282,P = 0.011）和罗伊氏粘液乳杆菌（t = 6.156,P = 0.003）相对丰度显著减少。与4weeks组相比,teadrink组小鼠肠道菌群分布差异大于control组。

红茶对新生幼鼠肠道菌群的形成能够产生影响,不同丰度的菌群可能通过调节碳水化合物代谢等途径来达到改善肠道菌群结构、增强肠道稳态的目的,具体代谢机制有待深入研究。

     

Effect of oral nalmefene, an opiate-receptor antagonist, on meal-stimulated gastric acid secretion and serum gastrin concentration in man

We evaluated whether nalmefene, an orally administered opiate-receptor antagonist, would inhibit gastric acid secretion in response to a meal in healthy humans. On separate days either 50 mg nalmefene or a placebo tablet was administered by mouth 90 min before a blenderized steak meal was infused into the stomach through a nasogastric tube. Compared to placebo, nalmefene inhibited meal-stimulated acid secretion in each of 6 subjects studied (P less than 0.05). During the second and third hours after the meal, nalmefene inhibited mean acid secretion by 16%. Nalmefene also resulted in significantly higher meal-stimulated serum gastrin concentrations than placebo (P less than 0.05) even though intragastric pH was kept constant at 5.0 in both experiments. These studies indicate that an orally administered opiate-receptor antagonist can inhibit gastric acid secretion in response to a meal in humans, yet increase meal-stimulated serum gastrin concentrations.     

Dual action of adiponectin on insulin secretion in insulin-resistant mice

Adiponectin is secreted by adipocytes and has been implicated as a mediator of insulin sensitivity. In this study, the acute effects of adiponectin on islets isolated from normal or diet-induced insulin resistant mice were examined. In normal islets, adiponectin (5 microg/ml) had no significant effect on insulin secretion. In contrast, in islets from mice rendered insulin resistant by high-fat feeding, adiponectin inhibited insulin secretion at 2.8 mM (P < 0.01) but augmented insulin secretion at 16.7 mM glucose (P < 0.05). The augmentation of glucose-stimulated insulin secretion by adiponectin was accompanied by increased glucose oxidation (P < 0.005), but without any significant effect on palmitate oxidation or the islet ATP/ADP ratio. Furthermore, RT-PCR revealed the expression of the adiponectin receptor AdipoR1 mRNA in mouse islets, however, with no difference in the degree of expression level between the two feeding groups. The results thus uncover a potential dual role for adiponectin to modify insulin secretion in insulin resistance.     

Localization of sodium absorption and chloride secretion in an intestinal epithelium

Summary Hen coprodeum absorbs sodium electrogenically and, when stimulated by theophylline, secretes chloride. In this study the vibrating microprobe technique was used to localize the transport of these ions to intestinal villi/folds and crypts. With the isolated, stretched epithelium, controlled by light microscopy and scanning electron microscopy, in open circuit, currents were inward, 40±7 A/cm², 50 m vertically above villi, and outward, 36±7 A/cm² above crypts. The currents decayed exponentially to near zero at 300 m with the same length constant. A physical model simulating the observed loci of current sources and sinks predicts potential profiles consistent with our data. Extrapolation of the currents gives a surface potential of 45 V, negative on villi and positive above crypts. Short circuiting increased villus current to 86±27 A/cm² at 50 m, and amiloride treatment reduced it to –8 A/cm²; in both cases crypt currents were abolished. The inward currents are compatible with sodium absorption. Induction of chloride secretion after amiloride treatment, resulted in current circuits similar to those induced by sodium absorption, with villus currents of 23±7 A/cm². This is in accord with chloride secretion at the villi. Quantitative estimates of crypt number (860/cm²) and opening diameter (15 m), in conjunction with isotopic measurements of active and electrical potential-driven ion fluxes demonstrate, however, that only 4% of the potential-driven co-ion transport occurs through the crypts. This indicates that nearly all chloride secretion comes from the sodium-absorbing villar area. Were the chloride secretion to occur solely from the crypts, the current should have been in the opposite direction and 10,000-fold larger.     

咖啡摄入量对小鼠肠道微生物及酶活性的影响

目的 研究摄入不同咖啡浓度对小鼠酶活性、肠道微生物、体重的影响,从分析肠道四种微生物变化和四种酶的活性变化来讨论摄入咖啡对健康的影响。方法 SPF级昆明小鼠随机分为4组,即低、中、高咖啡浓度组和正常对照组,所有实验组小鼠日常给予蒸馏水饮用,实验低、中、高浓度组每天2次分别灌胃给予0.0045 g/mL、0.0090 g/mL、0.0180 g/mL的咖啡溶液0.4 mL/（只•次）,对照组给予蒸馏水灌胃,实验时间为1个月,测小鼠体重、肠道微生物和酶活性等指标并进行分析。结果 与对照组相比,低浓度咖啡摄入组乳杆菌、双歧杆菌、大肠埃希菌数显著增加（P＜0.01或P＜0.05）,需氧和厌氧细菌数显著降低（P＜0.01）;中浓度咖啡摄入组乳杆菌、双歧杆菌数显著低于对照组和低浓度组（P＜0.01）,大肠埃希菌显著高于对照组（P＜0.05）;高浓度咖啡摄入组乳杆菌和大肠埃希菌总数显著低于对照组（P＜0.01或P＜0.05）;低浓度摄入组的木聚糖酶、纤维素酶、淀粉酶、蛋白酶的活性均显著高于（Ps＜0.01）对照组和中浓度咖啡摄入组;各组小鼠体重差异无统计学意义（P＞0.05）。结论 适当摄入咖啡小鼠肠道双歧杆菌和乳杆菌等益生菌数量增加显著,小鼠肠道中具有代表性的淀粉酶、纤维素酶、蛋白酶、木聚糖酶的活性均显著增加,但咖啡摄入对小鼠体重影响不显著。     

红茶对2型糖尿病小鼠肠道菌群分布的影响

探讨红茶对2型糖尿病（T2DM）小鼠肠道菌群分布的影响,为研究红茶在代谢性疾病的辅助治疗机制提供参考。

构建 T2DM小鼠模型,将其随机分为模型组（T2DM组）和茶水饲喂组（T2DM_T组）,取健康小鼠作为对照组（Control组）,每组各15只。实验终点收集3组小鼠粪便进行宏基因组测序,分析组间菌群差异;同时进行肝脏组织形态学检查,观察组织病理学改变。

与Control组和T2DM组相比,T2DM_T组拟杆菌门（F = 21.056,P＜0.001）、邓肯氏菌属（F = 13.330,P = 0.001）、乳杆菌属（F = 36.546,P＜0.001）和粘液乳杆菌属（F = 43.813,P＜0.001）的相对丰度增加,担子菌门（F = 8.676,P = 0.005）、Muribaculum（F = 8.151,P = 0.006）和肠球菌属（F = 4.271,P = 0.040）的相对丰度降低。与T2DM组小鼠相比,T2DM_T组小鼠肝细胞排列较为紧密,细胞空泡变性程度有所减弱。

红茶在一定范围内能调节T2DM模型小鼠肠道菌群结构和相对丰度,红茶水喂饲可减缓造模所致的T2DM小鼠肝脏细胞的损伤,其具体作用机制值得深入研究和探讨。

     

保和丸对食积小鼠的肠道微生物及酶活性的影响

目的 探究保和丸对食积小鼠肠道微生物及酶活性的影响。方法 将32只实验小鼠（雌雄各半）随机分为正常组（n=12）、食积组（n=10）和治疗组（n=10）。其中正常组喂食正常饲料,其余组喂食特制饲料。待食积组和治疗组中小鼠出现粪便干结,腹部膨隆胀满后,立即对治疗组按6.67 g/(kg·d)剂量灌胃0.28 g/mL的保和丸汤剂,正常组与食积组灌胃等量的蒸馏水。每天2次,每次0.35 mL,持续4 d。比较分析小鼠肠道微生物及肠道酶活性。结果 肠道微生物方面,食积组能显著增加细菌、乳酸菌、双歧杆菌和大肠埃希菌的数目,与正常组相比差异有统计学意义（均P0.05）。结论 保和丸能降低食积小鼠的细菌、乳酸菌以及大肠埃希菌的数目,同时能降低木聚糖酶、淀粉酶、蛋白酶和蔗糖酶等的活性。     

肠道菌群变化对实验小鼠肠黏膜免疫的影响

目的探讨肠道菌群变化对肠黏膜相关淋巴组织的影响。方法通过变性梯度凝胶电泳（Denatu-ring gradient gel electrophoresis,DGGE）法研究了三种不同级别实验小鼠即清洁级小鼠、SPF小鼠和普通小鼠肠道菌群的组成,并用免疫组织化学（immunohistochemistry,IHC）方法研究了此三种不同级别的实验小鼠肠黏膜相关淋巴组织sIgA阳性细胞分布情况。结果普通小鼠肠道细菌种类最多,其sIgA阳性细胞分布最多,肠道不同部位之间sIgA分布情况差异有显著性（P〈0.05）,小肠和大肠之间的阳性细胞分布差异极显著（P〈0.01）;其次是清洁级小鼠,其肠道不同部位之间菌种组成差异无显著性,小肠和大肠之间的阳性细胞分布差异有显著性（P〈0.05）;SPF小鼠肠道细菌种类最少,故其sIgA阳性细胞分布最少,且其肠道不同部位之间菌种组成差异无显著性,小肠和大肠之间的阳性细胞分布差异无显著性（P〉0.05）。结论随着动物微生物控制级别的增高,肠道微生物多样性递减;sIgA阳性细胞与肠道细菌种类正相关。     

芦笋对高脂饮食小鼠肠道微生物及酶活性的影响

目的 探明芦笋对高脂饮食小鼠肠道微生物及酶活性的影响情况,为芦笋的应用提供依据。方法 将实验动物随机分为正常组、高脂饮食组、高脂饮食低剂量芦笋组、高脂饮食中剂量芦笋组、高脂饮食高剂量芦笋组和高脂饮食阳性对照组。其中正常组喂食正常饲料,其余组喂食高脂饲料。分别灌胃蒸馏水（正常组、高脂饮食组）、芦笋1.05 g/（kg•d）（高脂饮食低剂量芦笋组）、芦笋2.10 g/（kg•d）（高脂饮食中剂量芦笋组）、芦笋4.20 g/（kg•d）（高脂饮食高剂量芦笋组）和降脂理肝汤1.19 g/（kg•d）（高脂饮食阳性对照组）,每天2次,每次0.35 mL。比较分析小鼠肠道微生物及肠道酶活性。结果 酶活方面,高脂饮食低剂量芦笋组、高脂饮食中剂量芦笋组、高脂饮食高剂量芦笋组能明显降低淀粉酶、木聚糖酶以及蛋白酶的活性,与高脂饮食组相比差异具有统计学意义（Ps<0.01）。肠道微生物方面,高脂饮食低剂量芦笋组、高脂饮食中剂量芦笋组、高脂饮食高剂量芦笋组能显著降低细菌、乳杆菌和双歧杆菌的数量。与高脂饮食组相比差异具有统计学意义（P<0.05或P<0.01）。结论 芦笋能降低高脂饮食小鼠的肠道内细菌、乳杆菌和双歧杆菌的数量,并且可以显著降低高脂饮食小鼠淀粉酶、木聚糖酶和蛋白酶等肠道酶的活性。     

Effect of beta-endorphin on gastric acid secretion and serum gastrin concentration in humans

The effect of synthetic human β-endorphin on gastric acid secretion was studied in 9 healthy subjects. Neither 2.5 mg or 15 mg β-endorphin had a significant effect on acid secretion or on serum gastrin concentration despite the fact that this dose of opiate caused a significant increase in serum prolactin concentrations. The role of endogenous opiate-like peptides on gastric secretion is discussed.     

超微铁皮石斛对脾虚便秘小鼠肠道微生物及酶活的影响

目的探讨超微铁皮石斛对脾虚便秘小鼠肠道微生物及酶活性的影响。方法采用灌胃番泻叶水煎液7 d后控制饮食,饥饱失常8 d,进行小鼠脾虚便秘造模。设模型组、正常组和治疗组,模型组和正常组灌胃无菌水,治疗组分别灌胃给药铁皮石斛传统汤剂、超微全量汤剂、超微50%量汤剂和超微25%量汤剂,共7 d,然后采集肠道内容物分析肠道菌群及酶活。结果模型组五类可培养微生物（细菌总数、大肠埃希菌、乳酸菌、双歧杆菌和真菌）数量与正常组比较差异均有统计学意义（P〈0.01）;铁皮石斛传统汤剂组大肠埃希菌数,铁皮石斛超微50%量汤剂组细菌总数、大肠埃希菌和真菌数,超微25%量汤剂组细菌总数和大肠埃希菌数均达到正常组水平（P〉0.05）;铁皮石斛超微全量汤剂组和超微50%量汤剂组对纤维素酶的影响作用相当,均达到正常组水平（P〉0.05）,铁皮石斛三种不同剂量的超微汤剂对木聚糖酶活性的影响作用相当（P〉0.05）,铁皮石斛超微全量汤剂组淀粉酶活性与正常组相当（P〉0.05）。结论铁皮石斛传统汤剂、超微全量汤剂和超微50%量汤剂对脾虚便秘均有很好的疗效,能够调控肠道微生态平衡及肠道酶活性。     

玉米紫色植株花色苷色素对小鼠肠道菌群的影响

目的了解玉米紫色植株花色苷色素对小鼠肠道菌群的影响。方法按中华人民共和国卫生部《保健食品检验与评价技术规范》中的动物实验调节肠道菌群功能的检验方法,观察玉米紫色植株色素对小鼠肠道中肠杆菌、乳杆菌、双歧杆菌数量的影响。结果灌服后小鼠肠道乳杆菌、双歧杆菌数量变化差异均无显著性,低剂量组肠扦菌数量明显低于灌服前（P〈0．05）,其他组肠杆菌数量变化差异无显著性。结论玉米紫色植株色素对小鼠肠道菌群可能有影响。     

养胃四君子茶对脾虚便秘小鼠肠道微生物、酶活性及胃动力的影响

目的探讨养胃四君子茶对脾虚便秘小鼠肠道微生物及酶活性的影响。方法将40只小鼠随机分为正常对照组、模型组、治疗组[养胃四君子茶(低剂量组、中剂量组、高剂量组)],每组8只。对模型组和治疗组采用灌胃番泻叶水提液7 d后,改用饮食不节+缺水燥结+过度疲劳喂养8 d等方法。造模结束后,模型组和正常对照组采用无菌水灌胃,治疗组用养胃四君子茶灌胃,0.8 mL/d连续6 d。然后采集肠道内容物,分析肠道菌群及酶活性。结果与正常对照组小鼠肠道细菌总数比较,模型组显著上升(P<0.05),治疗组差异无统计学意义(P>0.05);大肠埃希菌数量各组差异无统计学意义(P>0.05);小鼠肠道乳酸菌数量养胃四君子茶低、中剂量组均较正常对照组、模型组显著升高(均P<0.01);双歧杆菌数量养胃四君子茶低、中剂量组与正常对照组、模型组相比均显著升高(均P<0.01);与正常对照组比较,其余各组小鼠肠道酶活总体呈上升趋势,尤其肠道淀粉酶(P<0.01);养胃四君子茶低、中剂量组蛋白酶活性均显著上升(均P<0.05);与正常对照组比较,模型组及养胃四君子茶低、中剂量组木聚糖酶活性显著上升(P<0.01或P<0.05),其余组差异无统计学意义(P>0.05);与正常对照组比较,模型组及养胃四君子茶低、中剂量组纤维素酶活性均显著上升(均P<0.05)。与正常对照组比较,模型组、养胃四君子茶低剂量组的血清D 木糖含量显著下降(均P<0.01)。结论养胃四君子茶可抑制细菌过度增长、扶植有益菌生长,改善肠道酶活性、增强胃肠蠕动,从而改善脾虚便秘。     

Effects of CFTR gene silencing by siRNA or the luminal application of a CFTR activator on fluid secretion from guinea-pig pancreatic duct cells

Aims

The cystic fibrosis transmembrane conductance regulator (CFTR) is a cyclic AMP regulated chloride channel expressed in the apical plasma membrane of pancreatic duct cells where it plays an important role in fluid secretion. The purpose of this study was to elucidate the role of the CFTR chloride channel on ion and fluid secretion from the guinea-pig pancreas by manipulating the expression of CFTR by RNA interference or by luminal application of a CFTR selective activator, MPB91, in isolated cultured pancreatic ducts.

Materials and methods

Using cDNA isolated from the guinea-pig small intestine, fragments of the CFTR gene were generated by polymerase chain reaction and directly sequenced. Two different RNA duplexes for small interference RNA (siRNA) were designed from the sequence obtained. Fluid secretion from the isolated guinea-pig pancreatic ducts was measured using video-microscopy. The amount of CFTR chloride channel or AQP1 water channel expressed in pancreatic ducts was examined by immunoblotting with antibodies against CFTR or AQP1, respectively.

Results

Guinea-pig CFTR consists of 1481 amino acid residues. An additional glutamine residue was found to be inserted between amino acid residues 403 and 404 of human CFTR. Forskolin-stimulated fluid secretion from intact pancreatic ducts was significantly higher in the presence of MPB91 compared to fluid secretion in the absence of MPB91. Both basal and forskolin-stimulated fluid secretion in pancreatic ducts transfected with CFTR specific siRNAs were reduced by ∼50% compared to fluid secretion from ducts transfected with scrambled negative control dsRNAs. The amount of CFTR and AQP1 proteins was reduced to 34% and 45% of control, respectively.

Conclusions

The activity of the CFTR chloride channel or the amount of CFTR protein expressed determines the rate of fluid secretion from the isolated guinea-pig pancreatic ducts.     

运动对小鼠肠道菌群的影响

目的 利用PCR-DGGE方法比较运动小鼠和正常小鼠肠道菌群的结构和数量变化,研究运动对肠道菌群的影响。方法 从5只正常Balb/c小鼠和5只运动C57BL/6J小鼠的粪便中提取细菌基因组总DNA,用聚合酶链式反应—变性梯度凝胶电泳（PCR-DGGE）的方法获得小鼠肠道菌群分布图谱,对图谱进行相似性、多样性以及优势条带的序列分析。结果 PCR-DGGE结果图显示运动小鼠肠道菌群的多样性明显增加,优势菌群发生转变,序列分析表明变形菌门明显减少,厚壁菌门成为优势菌型。结论 运动会对小鼠肠道菌群产生影响。