共查询到20条相似文献,搜索用时 31 毫秒
1.
G. J. King F. H. Alston L. M. Brown E. Chevreau K. M. Evans F. Dunemann J. Janse F. Laurens J. R. Lynn C. Maliepaard A. G. Manganaris P. Roche H. Schmidt S. Tartarini J. Verhaegh R. Vrielink 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(5):699-708
Apple scab, caused by the fungus Venturia inaequalis (Cke.) Wint., is an important disease in commercial apple production. A mapping population of 155 individuals, derived from
a cross between the apple varieties ‘Prima’ (resistant)בFiesta’ (susceptible), was scored for response to the disease in
replicated field and glasshouse trials throughout Europe. Twenty data sets were selected and cluster analysis was used to
form a consensus score for the population fitting a 1 : 1 segregation ratio of resistance:susceptibility. The progeny were
scored with molecular markers. A detailed map covering 54 cM of the ‘Prima’ linkage group containing the Vf gene for scab resistance was constructed using 24 molecular markers linked to the resistance gene. One isoenzyme marker (Pgm-1), six RFLP markers and 17 RAPD markers formed a linkage group with the consensus measure of resistance to scab. Four marker
bridges were established with the corresponding ‘Fiesta’ linkage group with additional markers (one isozyme, one RFLP, three
RAPD and one AFLP). A low chi-square value indicated a good fit of the marker ordering, which was in close agreement with
previously reported linkage positions for some of the markers and Vf. Differences were observed in the ability of different scoring methods to resolve susceptible and resistant classes. The
results obtained for the consensus classification of resistance to scab for the population may suggest the presence of virulent
inocula at some sites, which could overcome the Vf gene for resistance. The consequences of relying on individual scoring occasions for studying Vf scab resistance are discussed in the context of linkage analysis, conventional breeding selection, and marker-assisted selection.
Received: 23 July 1997 / Accepted: 31 October 1997 相似文献
2.
Megumi Igarashi Yoshie Abe Yoshimichi Hatsuyama Takanori Ueda Tomoko Fukasawa-Akada Tomoyuki Kon Tsuyoshi Kudo Takashi Sato Masahiko Suzuki 《Molecular breeding : new strategies in plant improvement》2008,22(1):95-118
Two apple genetic linkage maps were constructed using amplified fragment length polymorphisms (AFLPs), simple sequence repeats
(SSRs), random amplified polymorphic DNAs (RAPDs), and expressed sequence tag (EST)-derived markers in combination with a
pseudo-testcross mapping strategy in which the cultivars ‘Ralls Janet’ and ‘Delicious’ were used as the respective seed parents.
Mitsubakaido (Malus sieboldii) was used as the pollen parent for each of the segregating F1 populations. Expressed sequence tag data were obtained from the random sequencing of cDNA libraries constructed from in vitro
cultured shoots and maturing fruits of cv ‘Fuji’, which is the offspring of a cross between ‘Ralls Janet’ and ‘Delicious’.
In addition, a number of published gene sequences were used to develop markers for mapping. The ‘Ralls Janet’ map consisted
of 346 markers (178 AFLPs, 95 RAPDs, 54 SSRs, 18 ESTs, and the S locus) in 17 linkage groups, with a total length of 1082 cM, while that of ‘Delicious’ comprised 300 markers (120 AFLPs,
81 RAPDs, 64 SSRs, 32 ESTs, and the S, Rf, and MdACS-1 loci) on 17 linkage groups spanning 1031 cM. These maps are amenable to comparisons with previously published maps of ‘Fiesta’
and ‘Discovery’ (Liebhard et al., Mol Breed 10:217–241, 2002; Liebhard et al., Theor Appl Genet 106:1497–1508, 2003a) because several of the SSRs (one to three markers per linkage group) were used in all of the maps. Distorted marker segregation
was observed in three and two regions of the ‘Ralls Janet’ and ‘Delicious’ maps, respectively. These regions were localized
in different parts of the genome from those in previously reported apple linkage maps. This marker distortion may be dependent
on the combinations of cultivars used for map construction. 相似文献
3.
QTL mapping of aroma compounds analysed by headspace solid-phase microextraction gas chromatography in the apple progeny ‘Discovery’ × ‘Prima’ 总被引:2,自引:0,他引:2
F. Dunemann D. Ulrich A. Boudichevskaia C. Grafe W. E. Weber 《Molecular breeding : new strategies in plant improvement》2009,23(3):501-521
Improving fruit quality of apple varieties is an important but complex breeding goal. Flavour is among the key factors of
apple fruit quality but in spite of the analytical and biochemical knowledge about volatiles little is known about the genetic
and molecular bases of apple aroma. The aim of this study was to use a saturated molecular linkage map of apple to identify
QTLs for aroma compounds such as alcohols, esters and terpenes, but also for a number of unidentified volatile compounds (non-targeted
analysis approach). Two parental genetic maps were constructed for the apple cultivars ‘Discovery’ and ‘Prima’ by using mainly
AFLP and SSR markers. ‘Discovery’ and ‘Prima’ showed very different volatile patterns, and ‘Discovery’ mostly had the higher
volatile concentrations in comparison with the Vf-scab resistant ‘Prima’ which has its origin in the small-fruited apple species Malus floribunda. About 50 putative QTLs for a total of 27 different apple fruit volatiles were detected through interval mapping by using
genotypic data of 150 F1 individuals of the mapping population ‘C3’ together with phenotypic data obtained by head-space solid phase microextraction
gas chromatography. QTLs for volatile compounds putatively involved in apple aroma were found on 12 out of the 17 apple chromosomes,
but they were not evenly dispersed. QTLs were mainly clustered on linkage groups LG 2, 3 and 9. In a first attempt, a LOX
(lipoxygenase) candidate gene, putatively involved in volatile metabolism, was mapped on LG 9, genetically associated with
a cluster of QTLs for ester-type volatiles. Implications for aroma breeding in apple are discussed. 相似文献
4.
A linkage map of the pea (Pisum sativum L.) genome containing cloned sequences of known function and expressed sequence tags (ESTs) 总被引:3,自引:0,他引:3
B. J. Gilpin J. A. McCallum T. J. Frew G. M. Timmerman-Vaughan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1289-1299
A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F2 plants produced by crossing the marrowfat cultivar ‘Primo’ and the blue-pea breeding line ‘OSU442-15’. This linkage map consists
of 209 markers and covers 1330 cM (Kosambi units) and includes RFLP, RAPD and AFLP markers. By mapping a number of anchor
loci, the ‘Primo’בOSU442-15’ map has been related to other pea linkage maps. A feature of the map is the incorporation of
29 loci representing genes of known function, obtained from other laboratories. The map also contains RFLP loci detected using
sequence-characterized cDNA clones developed in our laboratory. The putative identities of 38 of these cDNA clones were assigned
by examining public-sequence databases for protein or nucleotide-sequence similarities. The conversion of sequence-characterized
pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed
for single-copy sequences. Eleven polymorphic STSs were developed.
Received: 18 June 1997 / Accepted: 11 August 1997 相似文献
5.
RFLP and RAPD markers linked to the rosy leaf curling aphid resistance gene (Sd1) in apple 总被引:10,自引:0,他引:10
P. Roche F. H. Alston C. Maliepaard K. M. Evans R. Vrielink F. Dunemann T. Markussen S. Tartarini L. M. Brown C. Ryder G. J. King 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(3-4):528-533
Sd
1 is a dominant gene for resistance to biotypes 1 and 2 of the rosy leaf curling aphid, Dysaphis devecta Wlk., which can cause economic damage to apple trees. This report describes the identification of three RFLP and four RAPD
markers linked to Sd
1 in a cross between the D. devecta susceptible variety ‘Prima’ (sd
1
sd
1) and the resistant variety ‘Fiesta’ (Sd
1
sd
1). Potted trees were artificially infested in the glasshouse, and the ratio of resistant:susceptible plants supported the
hypothesis that the resistance was under the control of a single dominant gene. The position of the gene was mapped to a single
locus on a ‘Fiesta’ chromosome, within 2 cM of three tightly linked RFLP markers (MC064a, 2B12a and MC029b); the four RAPD
markers were located further away (between 13 and 46 cM). This is the first report of molecular markers for an aphid resistance
gene in tree fruit crops. The potential application of these markers in a marker-assisted resistance breeding programme is
discussed.
Received: 1 July 1996/Accepted: 23 August 1996 相似文献
6.
Analysis of complex leaf and flower characters in Rhododendron using a molecular linkage map 总被引:10,自引:0,他引:10
F. Dunemann R. Kahnau I. Stange 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1146-1155
A molecular linkage map of Rhododendron has been constructed by using a segregating population from an interspecific cross. Parent-specific maps based on 239 RAPD,
38 RFLP, and two microsatellite markers were aligned using markers heterozygous in both parents. The map of the male parent
‘Cunningham’s White’ comprised 182 DNA markers in 13 linkage groups corresponding to the basic chromosome number. In the female
parent ‘Rh 16’ 168 markers were located on 18 linkage groups. An assignment of putative homologous linkage groups was possible
for 11 groups of each parent. QTL analyses based on the non-parametric Kruskal-Wallis rank-sum test were performed for the
characters “leaf chlorosis” and “flower colour” scored as quantitative traits. For leaf chlorosis, two genomic regions bearing
QTLs with significant effects on the trait were identified on two linkage groups of the chlorosis-tolerant parent. RAPD marker
analysis of additional lime-stressed genotypes tested under altered environmental conditions verified the relationship between
marker allele frequencies and the expression of chlorosis. Highly significant QTL effects for flower colour were found on
two chromosomes indicating major genes located in these genome areas. The prospects for utilization of a linkage map in Rhododendron are discussed.
Received: 28 September 1998 / Accepted: 5 November 1998 相似文献
7.
Sibylle Stoeckli Karsten Mody Cesare Gessler Andrea Patocchi Mauro Jermini Silvia Dorn 《Tree Genetics & Genomes》2008,4(4):833-847
The rosy apple aphid (Dysaphis plantaginea), the leaf-curling aphid (Dysaphis cf. devecta) and the green apple aphid (Aphis pomi) are widespread pest insects that reduce growth of leaves, fruits and shoots in apple (Malus × domestica). Aphid control in apple orchards is generally achieved by insecticides, but alternative management options like growing
resistant cultivars are needed for a more sustainable integrated pest management (IPM). A linkage map available for a segregating
F1-cross of the apple cultivars ‘Fiesta’ and ‘Discovery’ was used to investigate the genetic basis of resistance to aphids.
Aphid infestation and plant growth characteristics were repeatedly assessed for the same 160 apple genotypes in three different
environments and 2 consecutive years. We identified amplified fragment length polymorphism (AFLP) markers linked to quantitative
trait loci (QTLs) for resistance to D. plantaginea (‘Fiesta’ linkage group 17, locus 57.7, marker E33M35–0269; heritability: 28.3%), and to D. cf. devecta (‘Fiesta’ linkage group 7, locus 4.5, marker E32M39–0195; heritability: 50.2%). Interactions between aphid species, differences
in climatic conditions and the spatial distribution of aphid infestation were identified as possible factors impeding the
detection of QTLs. A pedigree analysis of simple sequence repeat (SSR) marker alleles closely associated with the QTL markers
revealed the presence of the alleles in other apple cultivars with reported aphid resistance (‘Wagener’, ‘Cox’s Orange Pippin’),
highlighting the genetic basis and also the potential for gene pyramiding of aphid resistance in apple. Finally, significant
QTLs for shoot length and stem diameter were identified, while there was no relationship between aphid resistance and plant
trait QTLs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
8.
Sibylle Stoeckli Karsten Mody Andrea Patocchi Markus Kellerhals Silvia Dorn 《Tree Genetics & Genomes》2009,5(1):257-267
The aim of this study was to assess the genetic basis of rust mite (Aculus schlechtendali) resistance in apple (Malus × domestica). A. schlechtendali infestation of apple trees has increased as a consequence of reduced side effects of modern fungicides on rust mites. An
analysis of quantitative trait loci (QTLs) was carried out using linkage map data available for F1 progeny plants of the cultivars ‘Fiesta’ × ‘Discovery’. Apple trees representing 160 different genotypes were surveyed for
rust mite infestation, each at three different sites in two consecutive years. The distribution of rust mites on the individual
apple genotypes was aggregated and significantly affected by apple genotype and site. We identified two QTLs for A. schlechtendali resistance on linkage group 7 of ‘Fiesta’. The AFLP marker E35M42-0146 (20.2 cM) and the RAPD marker AE10-400 (45.8 cM) were
closest positioned to the QTLs and explained between 11.0% and 16.6% of the phenotypic variability. Additionally, putative
QTLs on the ‘Discovery’ chromosomes 4, 5 and 8 were detected. The SSR marker Hi03a10 identified to be associated to one of
the QTLs (AFLP marker E35M42-0146) was traced back in the ‘Fiesta’ pedigree to the apple cultivar ‘Wagener’. This marker may
facilitate the breeding of resistant apple cultivars by marker assisted selection. Furthermore, the genetic background of
rust mite resistance in existing cultivars can be evaluated by testing them for the identified SSR marker.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
9.
Construction of an integrated pepper map using RFLP,SSR, CAPS,AFLP, WRKY,rRAMP, and BAC end sequences 总被引:2,自引:1,他引:1
Heung-Ryul Lee Ik-Hyun Bae Soung-Woo Park Hyoun-Joung Kim Woong-Ki Min Jung-Heon Han Ki-Taek Kim Byung-Dong Kim 《Molecules and cells》2009,27(1):21-37
Map-based cloning to find genes of interest, markerassisted selection (MAS), and marker-assisted breeding (MAB) all require
good genetic maps with high reproducible markers. For map construction as well as chromosome assignment, development of single
copy PCR-based markers and map integration process are necessary. In this study, the 132 markers (57 STS from BAC-end sequences,
13 STS from RFLP, and 62 SSR) were newly developed as single copy type PCR-based markers. They were used together with 1830
markers previously developed in our lab to construct an integrated map with the Joinmap 3.0 program. This integrated map contained
169 SSR, 354 RFLP, 23 STS from BAC-end sequences, 6 STS from RFLP, 152 AFLP, 51 WRKY, and 99 rRAMP markers on 12 chromosomes.
The integrated map contained four genetic maps of two interspecific (Capsicum annuum ‘TF68’ and C. chinense ‘Habanero’) and two intraspecific (C. annuum ‘CM334’ and C. annuum ‘Chilsungcho’) populations of peppers. This constructed integrated map consisted of 805 markers (map distance of 1858 cM)
in interspecific populations and 745 markers (map distance of 1892 cM) in intraspecific populations. The used pepper STS were
first developed from end sequences of BAC clones from Capsicum annuum ‘CM334’. This integrated map will provide useful information for construction of future pepper genetic maps and for assignment
of linkage groups to pepper chromosomes. 相似文献
10.
F. Fernández-Fernández K. M. Evans J. B. Clarke C. L. Govan C. M. James S. Marić K. R. Tobutt 《Tree Genetics & Genomes》2008,4(3):587-479
Simple sequence repeat (SSR) markers developed from Malus, as well as Prunus, Pyrus and Sorbus, and some other sequence-tagged site (STS) loci were analysed in an interspecific F1 apple progeny from the cross ‘Fiesta’ × ‘Totem’ that segregated for several agronomic characters. A linkage map was constructed
using 259 STS loci (247 SSRs, four SCARs and eight known-function genes) and five genes for agronomic traits—scab resistance
(Vf), mildew resistance (Pl-2), columnar growth habit (Co), red tissues (Rt) and green flesh background colour (Gfc). Ninety SSR loci and three genes (ETR1, Rt and Gfc) were mapped for the first time in apple. The transferability of markers from other Maloideae to Malus was found to be around 44%. The loci are spread across 17 linkage groups, corresponding to the basic chromosome number of
Malus and cover 1,208 cM, approximately 85% of the estimated length of the apple genome. Interestingly, we have extended the top
of LG15 with eight markers covering 25 cM. The average map density is 4.7 cM per marker; however, marker density varies greatly
between linkage groups, from 2.5 in LG14 to 8.9 in LG7, with some areas of the genome still in need of further STS markers
for saturation.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
An erratum to this article can be found at 相似文献
11.
V. Laporte D. Merdinoglu P. Saumitou-Laprade G. Butterlin P. Vernet J. Cuguen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):989-996
The present study shows that the recently described mitochondrial H haplotype is associated with cytoplasmic male-sterility
(CMS). This new source of CMS appears to be different from the mitotype E-associated CMS most frequently found in natural
populations. A mitotype H progeny with a sexual phenotype segregation was used to identify a gene restoring male fertility
(R1H ). Using bulk segregant analysis (BSA), nine RAPD markers linked to this restorer locus were detected and mapped. The comparison
with other Beta genetic maps shows that the closest RAPD marker, distant from R1H by 5.2 cM, belongs to the same linkage group as the monogermy locus. In order to determine the position of R1H more precisely, four RFLP loci within this linkage group were mapped in the segregating progeny. It thus became possible
to construct a linkage map of the region containing the RFLP, RAPD and R1H loci. The closest RFLP marker was located 1.7 cM away from R1H. However, a nuclear gene restoring the ‘Owen’ CMS which is currently used in sugar beet breeding is reportedly linked to
the monogermy locus, raising the question of a possible identity between the new CMS system and the ‘Owen’ CMS.
Received: 15 September 1997 / Accepted: 1 December 1997 相似文献
12.
J. B. Clarke D. J. Sargent R. I. Bošković A. Belaj K. R. Tobutt 《Tree Genetics & Genomes》2009,5(1):41-51
One hundred and sixty microsatellite (simple sequence repeat (SSR)) and six gene-specific markers revealing 174 loci were
scored in 94 seedlings from the inter-specific cross of Prunus avium ‘Napoleon’ × Prunus nipponica accession F1292. The co-segregation data from these markers were used to construct a linkage map for cherry which spanned
680 cM over eight linkage groups with an average marker spacing of 3.9 cM per marker and just six gaps longer than 15 cM.
Markers previously mapped in Prunus dulcis ‘Texas’ × Prunus persica ‘Earlygold’ allowed the cherry map to be anchored to the peach × almond map and showed the high level of synteny between
the species. Eighty-four loci segregated in P. avium ‘Napoleon’ versus 159 in P. nipponica. The segregations of 32 isoenzyme loci in a subset of 47 seedlings from the progeny were scored, using polyacrylamide gel
electrophoresis and/or isoelectric focusing separation followed by activity staining, and the co-segregation data were analysed
along with those for 39 isoenzymes reported previously and for the 174 sequence-tagged site loci plus an additional two SSR
loci. The second map incorporates 233 loci and spans 736 cM over eight linkage groups with an average marker spacing of 3.2 cM
per marker and just two gaps greater than 15 cM. The microsatellite map will provide a useful tool for cherry breeding and
marker-assisted selection and for synteny studies within Prunus; the gene-specific markers and isoenzymes will be useful for comparisons with maps of other rosaceous fruit crops.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
13.
Marker-assisted selection (MAS) offers quick and reliable prediction of the phenotypes of seedlings in large populations and
thus opens new approaches for selection to breeders of apple (Malus x domestica Borkh.). The development of framework maps enables the discovery of genetic markers linked to desired traits. Although genetic
maps have been reported for apple scion cultivars, none has previously been constructed for apple rootstocks. We report the
construction of framework genetic maps in a cross between ‘M.9’ (‘Malling 9’) and ‘R.5’ (‘Robusta 5’) apple rootstocks. The
maps comprise 224 simple sequence repeat (SSR) markers, 18 sequence-characterised amplified regions, 14 single nucleotide
polymorphisms and 42 random amplified polymorphic DNAs. A new set of 47 polymorphic SSRs was developed from apple EST sequences
and used for construction of this rootstock map. All 17 linkage groups have been identified and aligned to existing apple
genetic maps. The maps span 1,175.7 cM (‘M.9’) and 1,086.7 cM (‘R.5’). To improve the efficiency of mapping markers to this
framework map, we developed a bin mapping set. Applications of these new genetic maps include the elucidation of the genetic
basis of the dwarfing effect of the apple rootstock ‘M.9’ and the analysis of disease and insect resistance traits such as
fire blight (Erwinia amylovora), apple scab (Venturia inaequalis) and woolly apple aphid (Eriosoma lanigerum). Markers for traits mapped in this population will be of direct use to apple breeders for MAS and for identification of
causative genes by map-based cloning. 相似文献
14.
Ana Delia Gisbert José Martínez-Calvo Gerardo Llácer María Luisa Badenes Carlos Romero 《Molecular breeding : new strategies in plant improvement》2009,23(3):523-538
Loquat [Eriobotrya japonica (Thunb.) Lindl.] is a Rosaceae fruit species of growing interest as an alternative to the main fruit crops. However, only
a few genetic studies have been carried out on this species. This paper reports the construction of the first genetic maps
of two loquat cultivars based on AFLP and microsatellite markers from Malus, Eriobotrya, Pyrus and Prunus genera. An F1 population consisting of 81 individuals, derived from the cross between ‘Algerie’ and ‘Zaozhong-6’ cultivars, was used to
construct both maps. A total of 111 scorable simple sequence repeat (SSR) loci resulted from the testing of 440 SSR primer
pairs in the analyzed progeny and the SSR transferability to Eriobotrya was found to be 74% from apple, 58% from pear and 49% from Prunus spp. In addition, 183 AFLP polymorphic bands were produced using 42 primer combinations. The ‘Algerie’ map was organized
in 17 linkage groups covering a distance of 900 cM and comprising 177 loci (83 SSRs and 94 AFLPs) with an average marker distance
of 5.1 cM. Self-incompatibility trait was mapped at the distal part of the LG17 linkage group, as previously reported in Malus and Pyrus. The ‘Zaozhong-6’ map covered 870 cM comprising 146 loci (64 SSRs and 82 AFLPs) with an average marker distance of 5.9 cM.
The 44 SSRs and the 48 AFLPs share in common by both maps were essentially collinear and, moreover, the order of the 75% of
apple and pear SSRs mapped in Eriobotrya was shown to be consistent across the Maloideae subfamily. As a whole, these maps represent a useful tool to facilitate loquat
breeding and an interesting framework for map comparison in the Rosaceae. 相似文献
15.
V. G. M. Bus D. Chagné H. C. M. Bassett D. Bowatte F. Calenge J.-M. Celton C.-E. Durel M. T. Malone A. Patocchi A. C. Ranatunga E. H. A. Rikkerink D. S. Tustin J. Zhou S. E. Gardiner 《Tree Genetics & Genomes》2008,4(2):223-236
Woolly apple aphid (WAA; Eriosoma lanigerum Hausm.) can be a major economic problem to apple growers in most parts of the world, and resistance breeding provides a sustainable
means to control this pest. We report molecular markers for three genes conferring WAA resistance and placing them on two
linkage groups (LG) on the genetic map of apple. The Er1 and Er2 genes derived from ‘Northern Spy’ and ‘Robusta 5,’ respectively, are the two major genes that breeders have used to date
to improve the resistance of apple rootstocks to this pest. The gene Er3, from ‘Aotea 1’ (an accession classified as Malus sieboldii), is a new major gene for WAA resistance. Genetic markers linked to the Er1 and Er3 genes were identified by screening random amplification of polymorphic deoxyribonucleic acid (DNA; RAPD) markers across DNA
bulks from resistant and susceptible plants from populations segregating for these genes. The closest RAPD markers were converted
into sequence-characterized amplified region markers and the genome location of these two genes was assigned to LG 08 by aligning
the maps around the genes with a reference map of ‘Discovery’ using microsatellite markers. The Er2 gene was located on LG 17 of ‘Robusta 5’ using a genetic map developed in a M.9 × ‘Robusta 5’ progeny. Markers for each of
the genes were validated for their usefulness for marker-assisted selection in separate populations. The potential use of
the genetic markers for these genes in the breeding of apple cultivars with durable resistance to WAA is discussed. 相似文献
16.
A molecular genetic map of cassava (Manihot esculenta Crantz) 总被引:12,自引:0,他引:12
M. Fregene F. Angel R. Gomez F. Rodriguez P. Chavarriaga W. Roca J. Tohme M. Bonierbale 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(3):431-441
A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers
segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM 2177-2’, elite cassava cultivars from Nigeria and Colombia, respectively. The
map consists of 20 linkage groups spanning 931.6 cM or an estimated 60% of the cassava genome. Average marker density is 1
per 7.9 cM. Since the mapping population is an F1 cross between heterozygous parents, with unique alleles segregating from either parent, a second map was constructed from
the segregation of 107 RFLPs, 50 RAPDs, 1 microsatellite, and 1 isoenzyme marker from the male parent. Comparison of intervals
in the male-and female-derived maps, bounded by markers heterozygous in both parents, revealed significantly less meiotic
recombination in the gametes of the female than in the male parent. Six pairs of duplicated loci were detected by low-copy
genomic and cDNA sequences used as probes. Efforts are underway to saturate the cassava map with additional markers, to join
the male- and female-derived maps, and to elucidate genome organization in cassava.
Received: 5 July 1996/Accepted: 22 November 1996 相似文献
17.
Development of a new SSR-based linkage map in apricot and analysis of synteny with existing Prunus maps 总被引:1,自引:0,他引:1
L. Dondini O. Lain F. Geuna R. Banfi F. Gaiotti S. Tartarini D. Bassi R. Testolin 《Tree Genetics & Genomes》2007,3(3):239-249
Linkage maps of the apricot accessions ‘Lito’ and ‘BO 81604311’ were constructed using a total of 185 simple sequence repeat
(SSR) markers sampled from those isolated in peach, almond, apricot and cherry; 74 were derived from a new apricot genomic
library enriched for AG/CT microsatellite repeats (UDAp series), and in total, 98 had never been mapped in Prunus before. Eight linkage groups putatively corresponding to the eight haploid apricot chromosomes were identified for each parent.
The two maps were 504 and 620 cM long, respectively, with 96 anchor markers showing a complete co-linearity between the two
genomes. As few as three gaps larger than 15 cM were present in ‘Lito’ and six in the male parent; the maps align well with
all the available SSR-based Prunus maps through the many common anchor loci. Only occasionally inverted positions between adjacent markers were found, and this
can be explained by the small size of cross populations analysed in these Prunus maps and in those reported in literature. The newly developed apricot SSRs will help saturating the existing Prunus maps and will extend the choice of markers in the development of genetic maps for new breeding populations. 相似文献
18.
Identification of molecular markers linked to quantitative trait loci for soybean resistance to corn earworm 总被引:7,自引:0,他引:7
B. G. Rector J. N. All W. A. Parrott H. R. Boerma 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):786-790
One hundred and thirty nine restriction fragment length polymorphisms (RFLPs) were used to construct a soybean (Glycine max L. Merr.) genetic linkage map and to identify quantitative trait loci (QTLs) associated with resistance to corn earworm (Helicoverpa zea Boddie) in a population of 103 F2-derived lines from a cross of ‘Cobb’ (susceptible) and PI229358 (resistant). The genetic linkage map consisted of 128 markers
which converged onto 30 linkage groups covering approximately 1325 cM. There were 11 unlinked markers. The F2-derived lines and the two parents were grown in the field under a plastic mesh cage near Athens, Ga., in 1995. The plants
were artificially infested with corn earworm and evaluated for the amount of defoliation. Using interval-mapping analysis
for linked markers and single-factor analysis of variance (ANOVA), markers were tested for an association with resistance.
One major and two minor QTLs for resistance were identified in this population. The PI229358 allele contributed insect resistance
at all three QTLs. The major QTL is linked to the RFLP marker A584 on linkage group (LG) ‘M’ of the USDA/Iowa State University
public soybean genetic map. It accounts for 37% of the total variation for resistance in this cross. The minor QTLs are linked
to the RFLP markers R249 (LG ‘H’) and Bng047 (LG ‘D1’). These markers explain 16% and 10% of variation, respectively. The
heritability (h2) for resistance was estimated as 64% in this population.
Received: 15 October 1997 / Accepted: 4 November 1997 相似文献
19.
Muhammad A. Khan Brion Duffy Cesare Gessler Andrea Patocchi 《Molecular breeding : new strategies in plant improvement》2006,17(4):299-306
Fire blight caused by the bacterium Erwinia amylovora is a severe threat to apple and pear orchards worldwide. Apple varieties exhibit a wide range of relative susceptibility/tolerance to fire blight. Although, no monogenic resistance against fire blight has been identified yet, recent evidence indicates the existence of quantitative resistance. Potential sources of fire blight resistance include several wild Malus species and some apple cultivars. F1 progenies of ‘Fiesta’בDiscovery’ were inoculated with the Swiss strain Ea 610 and studied under controlled conditions to identify quantitative trait loci (QTLs) for fire blight resistance. Disease was evaluated at four time points after inoculation. Shoot lesion length and the area under disease progress curve (AUDPC) values were used for QTL analysis. One significant (LOD score of 7.5–8.1, p<0.001) QTL was identified on the linkage group 7 of ‘Fiesta’ (F7). The F7 QTL explained about 37.5–38.6% of the phenotypic variation. 相似文献
20.
Sargent DJ Passey T Surbanovski N Lopez Girona E Kuchta P Davik J Harrison R Passey A Whitehouse AB Simpson DW 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(7):1229-1240
The linkage maps of the cultivated strawberry, Fragaria × ananassa (2n = 8x = 56) that have been reported to date have been developed predominantly from AFLPs, along with supplementation with transferrable
microsatellite (SSR) markers. For the investigation of the inheritance of morphological characters in the cultivated strawberry
and for the development of tools for marker-assisted breeding and selection, it is desirable to populate maps of the genome
with an abundance of transferrable molecular markers such as microsatellites (SSRs) and gene-specific markers. Exploiting
the recent release of the genome sequence of the diploid F. vesca, and the publication of an extensive number of polymorphic SSR markers for the genus Fragaria, we have extended the linkage map of the ‘Redgauntlet’ × ‘Hapil’ (RG × H) mapping population to include a further 330 loci,
generated from 160 primer pairs, to create a linkage map for F. × ananassa containing 549 loci, 490 of which are transferrable SSR or gene-specific markers. The map covers 2140.3 cM in the expected
28 linkage groups for an integrated map (where one group is composed of two separate male and female maps), which represents
an estimated 91% of the cultivated strawberry genome. Despite the relative saturation of the linkage map on the majority of
linkage groups, regions of apparent extensive homozygosity were identified in the genomes of ‘Redgauntlet’ and ‘Hapil’ which
may be indicative of allele fixation during the breeding and selection of modern F. × ananassa cultivars. The genomes of the octoploid and diploid Fragaria are largely collinear, but through comparison of mapped markers on the RG × H linkage map to their positions on the genome
sequence of F. vesca, a number of inversions were identified that may have occurred before the polyploidisation event that led to the evolution
of the modern octoploid strawberry species. 相似文献