Structure of Phosphorylated SF1 Bound to U2AF65 in an Essential Splicing Factor Complex

1. Download : Download high-res image (401KB)
2. Download : Download full-size image

Highlights? Splicing factor SF1 phosphorylation on a conserved SPSP motif is required in vivo ? SPSP phosphorylation (P) induces local folding within an SF1/U2AF⁶⁵ interface ? Phosphorylation promotes an acutely bent (P)SF1/U2AF⁶⁵/RNA conformation     

Substrate recognition mechanism of atypical protein kinase Cs revealed by the structure of PKCι in complex with a substrate peptide from Par-3

1. Download : Download high-res image (151KB)
2. Download : Download full-size image

Highlights? The 3D structure of PKCι in complex with a peptide from Par-3 is determined ? PKCι does not need phosphorylation of Thr402 in its activation loop for its activity ? aPKCs do not need a priming kinase for their activations ? The PKCι/Par-3 structure reveals a unique substrate recognition patterns for aPKCs     

Conversion of a Signal into Forces for Axon Outgrowth through Pak1-Mediated Shootin1 Phosphorylation

1. Download : Download high-res image (236KB)
2. Download : Download full-size image

Highlights? An attractive guidance cue, netrin-1, induces Pak1-mediated shootin1 phosphorylation ? Pak1-mediated shootin1 phosphorylation enhances clutch engagement at growth cones ? Pak1-mediated shootin1 phosphorylation promotes formation of traction forces ? Pak1-mediated shootin1 phosphorylation promotes axon outgrowth     

IKKε regulates cell elongation through recycling endosome shuttling

1. Download : Download high-res image (412KB)
2. Download : Download full-size image

Highlights? Recycling endosomes shuttle along the proximal-distal axis of growing bristles ? IKK? locally antagonizes Rab11 effector Nuf at the bristle tip by phosphorylation ? Nuf-endosome pathway is independent of actin cytoskeleton or DIAP1-caspase pathway ? Mammalian IKK-related kinases antagonize Rab11-FIP3 through phosphorylation     

The Structure of the Tiam1 PDZ Domain/ Phospho-Syndecan1 Complex Reveals a Ligand Conformation that Modulates Protein Dynamics

1. Download : Download high-res image (206KB)
2. Download : Download full-size image

Highlights? The Tiam1 PDZ domain preferentially binds a subset of syndecan (SDC) proteins ? The PDZ domain/phospho-SDC1 structure reveals a phosphoryl binding pocket ? Ligand specificity and phosphorylation regulate PDZ/SDC interactions and signaling ? Tiam1 PDZ domain dynamics are ligand-dependent and regulated by phosphorylation     

Disease mutations in the ryanodine receptor central region: crystal structures of a phosphorylation hot spot domain

1. Download : Download high-res image (294KB)
2. Download : Download full-size image

Highlights? Crystal structures of a central RyR domain contain phosphorylation sites ? Eleven RyR1 disease mutations cluster on two opposite faces of the domain ? The RyR1 L2867G mutation causes a drastic destabilization ? In vitro phosphorylation identifies 5 PKA and 4 CaMKII targets     

Moonlighting by different stressors: crystal structure of the chaperone species of a 2-Cys peroxiredoxin

1. Download : Download high-res image (272KB)
2. Download : Download full-size image

Highlights? The Schistosoma mansoni peroxiredoxin I (SmPrxI) is a moonlighting protein ? SmPrxI switches from LMW peroxidase to HMW chaperone due to chemical stressors ? The three-dimensional structures of both LMW and HMW SmPrxI have been solved ? SmPrxI HMW is the structure of a Prx chaperone     

The Retroviral Restriction Ability of SAMHD1, but Not Its Deoxynucleotide Triphosphohydrolase Activity,Is Regulated by Phosphorylation

1. Download : Download high-res image (130KB)
2. Download : Download full-size image

Highlights? SAMHD1 only exhibits antiviral activity when expressed in noncycling cells ? SAMHD1 is unphosphorylated in noncycling cells ? Reverse genetics revealed that phosphorylated SAMHD1 is repressive for restriction ? SAMHD1 phosphorylation regulates restriction, but not the cellular levels of dNTPs     

Inhibition of protein phosphatase 2A activity by PI3Kγ regulates β-adrenergic receptor function

1. Download : Download high-res image (376KB)
2. Download : Download full-size image

Highlights? PI3Kγ inhibits βAR resensitization by regulating PP2A activity ? PI3Kγ inhibits PP2A activity by phosphorylating I2PP2A on Serine 9 and 93 ? Phosphorylation of I2PP2A leads to its agonist-mediated interaction with PP2A ? siRNA-targeted depletion of I2PP2A results in βAR resensitization     

Structure and Dynamics of the Second CARD of Human RIG-I Provide Mechanistic Insights into Regulation of RIG-I Activation

1. Download : Download high-res image (154KB)
2. Download : Download full-size image

Highlights? The structure of a CARD of human RIG-I ? The mechanism and structural role of phosphorylation are revealed ? In trans interaction with the Helicase and regulatory domains is shown ? Lys172 lies in proximity to the CARD2:helicase-CTD interface     

Syndecan-4 Phosphorylation Is a Control Point for Integrin Recycling

1. Download : Download high-res image (288KB)
2. Download : Download full-size image

Highlights? c-Src phosphorylates syndecan-4 in response to extracellular stimuli ? Syndecan-4 phosphorylation and engagement regulate Arf6 activity ? Syndecan-4-mediated Arf6 activity regulates differential integrin recycling ? Syndecan-4-mediated integrin recycling controls FA dynamics and cell migration     

An OsCEBiP/OsCERK1-OsRacGEF1-OsRac1 Module Is an Essential Early Component of Chitin-Induced Rice Immunity

1. Download : Download high-res image (195KB)
2. Download : Download full-size image

Highlights? Chitin induces rapid activation of the rice small GTPase OsRac1 at the plasma membrane ? OsRacGEF1 interacts with and acts as a guanine nucleotide exchange factor for OsRac1 ? In response to chitin, OsRacGEF1 is activated via phosphorylation by OsCERK1 ? OsRacGEF1 is required for chitin-driven immunity and resistance to rice blast fungus     

HSF-1 regulators DDL-1/2 link insulin-like signaling to heat-shock responses and modulation of longevity

1. Download : Download high-res image (260KB)
2. Download : Download full-size image

Highlights? C. elegans DAF-2 insulin-like signaling negatively regulates HSF-1 activity ? DDL-1 and DDL-2 are negative regulators of HSF-1 ? Formation of a complex containing DDL-1/2 and HSF-1 inhibits HSF-1 activity ? DAF-2 signaling promotes DHIC formation by inhibiting DDL-1 phosphorylation     

A dynamic complex of signaling proteins uses polar localization to regulate cell-fate asymmetry in Caulobacter crescentus

1. Download : Download high-res image (291KB)
2. Download : Download full-size image

Highlights? DivL, an unorthodox kinase, promotes activation of the master kinase CckA ? Phosphorylated DivK binds directly to DivL to block the activation of CckA ? Cell cycle and cellular asymmetry are driven by changes in DivK phosphorylation ? Full activation of CckA depends on polar localization with a DivK phosphatase, PleC     

Regulation of the CRL4Cdt2 Ubiquitin Ligase and Cell-Cycle Exit by the SCFFbxo11 Ubiquitin Ligase

1. Download : Download high-res image (391KB)
2. Download : Download full-size image

Highlights? FBXO11 targets CDT2, a CRL4 substrate receptor, for proteasomal degradation ? CDK-mediated phosphorylation of CDT2 degron inhibits recognition by FBXO11 ? FBXO11-mediated degradation of CDT2 controls the timing of cell-cycle exit ? FBXO11-CDT2 functional interaction is evolutionary conserved from worms to humans     

Riboswitch Control of Aminoglycoside Antibiotic Resistance

1. Download : Download high-res image (257KB)
2. Download : Download full-size image

Highlights? The 5′ leader RNA of aminoglycoside antibiotic-resistance genes is conserved ? Aminoglycosides induce reporter gene expression by interacting with the leader RNA ? The aminoglycosides bind to the leader RNA and induce a change in the RNA structure ? Induction is independent of leader peptide stalling or drug-ribosome interactions     

LSD2/KDM1B and Its Cofactor NPAC/GLYR1 Endow a Structural and Molecular Model for Regulation of H3K4 Demethylation

1. Download : Download high-res image (277KB)
2. Download : Download full-size image

Highlights? Identifying NPAC as a novel LSD2 cofactor stimulating H3K4 demethylation ? Structure determination of LSD2 alone or in complex with NPAC and histone H3 peptide ? Defining the key NPAC residues essential for its intrinsic LSD2 cofactor activity ? Establishing a molecular model for how a cofactor regulates histone demethylation