Influence of a Permanent Magnetic Field on the Composition and Content of Sugars in Leaves and Storage Roots of Radish Plants of Major Types of Magnetic Orientation

The influence of a weak permanent magnetic field (PMF) with a strength of 400 A/m on the composition and content of sugars was investigated in radish (Raphanus sativus L.) leaves and storage roots of the two major types of magnetic orientation (TMO): North–South (NS TMO) and West–East (WE TMO). Plants were grown in spring and autumn in a greenhouse of the Institute of Plant Physiology, Russian Academy of Sciences. The weak PMF changed the total sugar content in the leaves and storage roots of radish depending on the belonging of the radish plants to the NS or WE type of magnetic orientation, the growing season, and age of plants and the plant organ. The PMF was an environmentally significant factor, since radish plants of different types of magnetic orientation (NS and WE) had a different reaction to the effect of the field related to the peculiarities of their physiological status.     

Effect of Choline Chloride on the Lipid Content and Composition in the Leaves of Principal Magnetically-Oriented Radish Types

The effects of radish seed treatment with choline chloride on the number and weight of leaves, the weight of roots, as well as the content and composition of polar and neutral lipids and their component fatty acids in the leaves of principal magnetically-oriented types (MOTs) of radish (cv. Rosovo-krasnyi s belym konchikom), that is, North–South (NS) and West–East (WE) ones, were investigated. It was shown that seed treatment with 1% choline chloride increased the proportion of WE MOT in plant population. In spring and in autumn, this treatment increased the weight of roots of the NS MOT plants, but did not affect this index in the WE MOT plants. In the spring NS MOT plants, choline chloride treatment did not change the absolute content of polar lipids as compared to the control, but in autumn, this index increased. Meanwhile, in the WE MOT plants, it increased in spring and did not change in autumn. In the spring NS MOT plants, the content of neutral lipids increased, but in the autumn plants, it did not change. At the same time, in the WE MOT plants, this index decreased both in spring and in autumn. Seed treatment with choline chloride resulted in a substantial increase in the total content of phospholipids, in particular, that of phosphatidylcholine and phosphatidylethanolamine, in the NS and WE MOT plants sampled both in spring and in autumn. In addition, the ratio of unsaturated to saturated fatty acids, as well as that of linolenic to linoleic fatty acids, somewhat increased in the NS MOT leaf lipids, but decreased in the WE MOT ones. Presowing treatment of radish seeds with choline chloride variously and sometimes oppositely affected the content and composition in the NS and WE MOT leaf lipids. This seems to be caused by different response of these MOTs to the environmental factors.     

Lipid composition and content in the seeds of radish plants of different magnetic orientation grown in weak permanent magnetic field

The content and composition of lipids were studied in the seeds of radish plants (Raphanus sativus L. var. radicula D.C., cv. Rosovo-krashyi s belym konchikom) grown from “seed to seed” in 2008 and 2009 in the greenhouse of the Institute of Plant Physiology in a permanent horizontal magnetic field (PMF) of Helmholz coils with the strength of ～400 A/m, in soil culture, at natural day length, and a temperature changing during the day. PMF suppressed all stages of radish plant development, from the appearance of alternative leaves to the formation of pods and mature seeds. In plants of the North-South magnetically oriented type (NS MOT), PMF reduced the number and weight of seeds; in the West-East magnetically oriented type (WEMOT), the number of seeds was reduced but their weights increased. In the seeds of the first generation of NS MOT, the total lipid content was higher than in the seeds of WE MOT. The amount of polar lipids in the seeds of NS MOT increased, whereas in the seeds of WE MOT it decreased or remained unchanged as compared with control. The content of neutral lipids reduced in both plant types. The strongest changes in the fatty acid composition of lipids with the highest content of unsaturated fatty acids were observed in the seeds of WE MOT in 2008. The weak PMF-induced differences in the changes of lipid composition and content in the seeds of different MOTs were evidently determined by seed sensitivity to the direction of field action. It is suggested that the occurrence of different MOTs increases the tolerance of plant population to unfavorable environmental factors, thus affecting its survival.     

The effects of a weak permanent magnetic field on the lipid composition and content in the onion leaves of various ages

We have studied the effects of a weak permanent magnetic field (PMF) with strength of 403 A/m on the composition and content of polar and neutral lipids and the composition of their fatty acids (FAs). The lipids were isolated from the third, fourth, and fifth leaves of onion (Allium cepa L., cv. Arzamasskii) plants, and their composition was determined using TLC and GLC techniques. Plants growth under the conditions of a natural geomagnetic field served as a control. Most intense changes in the lipid content induced by PMF were observed in the fourth onion leaf. The content of total lipids and that of polar lipids (glyco-and phospholipids) changed, whereas the content of neutral lipids either decreased or remained unchanged. The phospholipid/sterol ratio increased, causing an increase in the fluidity of the membrane lipid bilayer. PMF induced an increase in the concentration of linolenic acid and the relative content of total unsaturated FAs. The effects of PMF on the content and composition of lipids in the third and fifth onion leaves were less pronounced, demonstrating differences between the leaves of various ages in their sensitivity to the effects of magnetic field. It is concluded that changes in the weak PMF within the limits of changes in the strength of geomagnetic field in the course of evolution can affect biochemical and physiological processes of plants.     

Effect of alternating magnetic field on ontogenesis and morphophysiological characteristics of radish plants of different magnetic orientation

Effect of weak horizontal alternating magnetic field (AMF) with a frequency of 50 Hz and intensity of 400 A/m on seed formation and morphophysiological characteristics was investigated in radish (Raphanus sativus L.) plants of major types of magnetic orientation (TMO): North–South (NS) and West–East (WE). AMF retarded the passage through all the stages of ontogenesis; as compared with control material, the next leaves emerged slower, and the transition to formation of flower-bearing stems, budding, flowering, and production of pods and mature seeds was delayed. In plants of NS TMO exposed to AMP, the number of pods and seeds and the weight of seeds decreased, while these characteristics rose in WE TMO. AMF acted as an environmental factor differentiating plants’ response depending on their type of magnetic orientation. Dissimilar response to the magnetic field is associated with their physiological status.     

Effect of alternating magnetic field on the composition and level of lipids in radish seedlings

The effects of alternating magnetic field (AMF) with the frequency of 50 Hz on the dynamics of unfolding of cotyledon leaves, the composition and level of polar and neutral lipids and their component fatty acids (FA) were studied in 5-day-old radish seedlings (Raphanus sativus L. var. radicula D.L., cv. Rozovo-krasnyi s belym konchikom) grown in the light and in the dark. AMF weakened the inhibitory effect of light on unfolding of cotyledon leaves. In the light, the total content of lipids, as well as the level of polar and neutral lipids, in the seedlings in AMF was greater than in control material. In polar lipids, the total amount of glyco-and phospholipids increased; in neutral lipids, the level of triacylglycerols rose. The ratio between phospholipids and sterols (PhL/S) increased. In the dark, the total content of lipids and the level of neutral lipids in the seedlings in AMF were lower than in control material, and the ratio PhL/S decreased. In control material, there were no differences in the relative total content of unsaturated FA in the light and in the dark, whereas the level of linolenic acid was higher in the light than in the dark. AMF induced a decrease in the content of linolenic acid in the light and a rise in the dark; the level of erucic acid in the light decreased. The ratio between unsaturated and saturated FA decreased both in the light and in the dark. It was concluded that AMF with the frequency of 50 Hz was an adjusting agent considerably changing the content of lipids in the radish seedlings in the light and in the dark.     

Effect of weak constant magnetic field on the composition and content of lipids in radish seedlings at various temperatures

We studied the effects of weak permanent homogenous hirizontal magnetic field (PMF) (400 A/m) on the composition and content of lipids and composition of their fatty acids (FAs) in radish (Raphanus sativus L. var. radicula D.C., cv. Rosovo-krashyi s belym konchikom) seedlings at temperatures of 20 and 10°C. We compared lipid composition and content in seedlings at the phase of developed cotyledons (20°C, 5-day-old, and 10°C, 8-day-old seedlings) under low light and in darkness with the lipid composition and content in dry seeds. The seedlings grown in geomagnetic field (GMF) served as a control. In dry seeds, about 99% of total lipids comprised neutral lipids (NL) and only 1% were polar lipids (PL). Triacylglycerols predominated among NL comprising 93% of total seed lipids. During seed germination, NLs were consumed and PL were produced: the amount of glycolipids increased in control by 3.5–5 times and the amount of phospholipis, by 1.5–2 times.In the light at 20°C, PMF suppressed the formation of PL (by 18%), whereas in darkness, it stimulated it approximately by 80% as compared with control. In the light at 10°C, PMF slightly stimulated PL formation; in darkness, it did not almost affect their synthesis. In all treatments, PMF increased the ratio of phospholipids to sterols by 30–100%. Among FA, PMF exerted the strongest effect on the content of erucic acid: it increased in the light and in darkness at 20°C approximately by 25% and decreased at 10°C in the light by 13%. PMF behaved as a correction factor affecting lipid metabolism on the background of light and temperature action.     

Fatty acid composition of lipids from the vacuolar membranes of the roots of root vegetables

The fatty acid (FA) composition of vacuolar membrane lipids from the storage tissues of parsnip (Pastinaca sativa L.), parsley (Petroselinum crispum L.), and carrot (Daucus carota L.) was studied by gasliquid chromatography, and possible pathways of the biosynthesis of these acids are considered. A high level of unsaturated FAs (up to 78% of the total FA amount) was characteristic of these membrane lipids with the predominance of linoleic acid, which content in vacuolar lipids of parsnip, parsley, and carrot was 53.5, 55.1, and 54.4%, respectively. A high content of hexadienoic acid (C16:2ω6) was characteristic of the vacuolar lipids of parsnip and parsley (8.0 and 4.6%. respectively); the content of α-linolenic acid in the vacuolar lipids of these plants was 4.4–7.3%. Palmitic acid predominated among the saturated FAs (18.0–20.4%).     

Effect of extremely low frequency magnetic fields on the seedlings of wild plants growing in Central Yakutia

It was shown that permanent (B = 50 μT, horizontal plane, direction to the north) and alternating magnetic fields (North–South direction) exerted influences on seed germination as well as on cytological and biochemical features of seedlings characteristic of investigated species (Lepidium apetalum, Artemisia vulgaris, A. jacutica, and A. dracunculus) of wild plants growing in Central Yakutia. Under the effect of permanent magnetic field (MF), germinating capacity of seeds decreased (except for A. vulgaris), whereas alternating MF of different frequencies improved their germinating capacity, except for L. apetalum and A. jacutica at frequencies of 200 and 300 Hz, respectively. Under permanent MF, the rate of lipid peroxidation in the tissues of the seedlings decreased, whereas the content of low molecular weight antioxidants rose; when the plants were exposed to an alternating magnetic field, the content of MDA and peroxidase activity increased, and the content of low molecular weight antioxidants followed an ambiguous pattern.     

Fatty Acid Composition of Lipids from Leaves and Strobila of Cycas Revoluta (<Emphasis Type="Italic">Cycas revoluta</Emphasis>)

Fatty acid (FA) composition of lipids from leaves and differentiated fleshy strobila tissues and sporangia with spores of Cycas (Cycas revoluta Thumb.) after their step quantitative extraction from plant material was investigated. Quantitative content and qualitative composition of FAs of extractable and nonextractable leaf lipids were determined. It was established that flesh lipids of sporophylls are characterized by a high saturation level and contain a considerable proportion of saturated FAs with the usual chain length (C₁₂–C₁₈, 53–57%). At the same time, total amount of etherified FAs with a very long chain in lipids not extractable by the method of Zhukov and Vereshchagin exceeds several times that found in extractable lipids (~15 and ~4%, respectively). Neutral lipids of Cycas spores were represented by triacylglycerols, the lower-alkyl esters of FAs, free FAs, and sterol esters.     

Growth of Green Onions in a Weak Permanent Magnetic Field

Effect of a weak permanent magnetic field (PMF) with a strength of 403 A/m on Allium cepaL. bulb sprouting and leaf growth was investigated. Two onion varieties to produce green onions (cv. Arzamasskii) and bulb onions (cv. Ryazanskii) were examined. In addition, the content of chlorophyll, carbohydrates, and protein in the leaves of the control and PMF-treated plants were determined. The plants of the control group were grown under a natural geomagnetic field. The treatment of onions with PMF accelerated sprouting and extended the length of the fourth leaf in cv. Arzamasskii as much as 40%; the first leaf in cv. Ryazanskii was lengthened by 25% with respect to its length in untreated plants. Exposure to PMF increased the number of sprouts in cv. Ryazanskii and the number of sprout bunches in cv. Arzamasskii. In addition, PMF elevated the total content of chlorophyll and protein, expressed per fresh weight of green onions, but had no effect on the total content of carbohydrates. Conversely, PMF reliably reduced the total content of chlorophyll and protein in cv. Ryazanskii. The temperature increase diminished the effects of PMF.     

Changes in the fatty acid composition of symbiotic dinoflagellates from the hermatypic coral <Emphasis Type="Italic">Echinopora lamellosa</Emphasis> during adaptation to the irradiance level

The composition of fatty acids (FAs) of symbiotic dinoflagellates isolated from the hermatypic coral Echinoporal lamellosa adapted to the irradiance of 95, 30, 8, and 2% PAR was studied. Polar lipids and triacylglycerols (TAG) differed between them in FA composition. Polar lipids were enriched in unsaturated FAs, whereas TAG, in saturated FAs. Light exerted a substantial influence on the FA composition in both polar lipids and TAG. The elevation of irradiance resulted in the accumulation of 16:0 acid in both lipid groups and 16:1(n-7) acid in TAG. It seems likely that de novo synthesis of 16:0 acid occurred actively in the cells of symbiotic dinoflagellates in high light. Since these processes are energy-consuming ones, they utilize excessive energy. When light intensity declined, 18:4(n-3) and 20:5(n-3) acids accumulated in polar lipids, which was accompanied by the increase in the content of chlorophyll a in the cells of zooxanthellae, whereas the levels of 22:6(n-3) and 20:4(n-6) acids reduced. Although the relative content of particular FAs varied substantially in dependence of irradiance, the balance between the sum of saturated and unsaturated FAs changed insignificantly. We concluded that the role of photoadaptation could not be limited only to changes in the degree of lipid unsaturation and membrane fluidity. It is supposed that light-induced changes in the FA composition reflect the interrelation between photosynthesis and FA biosynthesis.     

Lipid fatty acid composition of potato plants transformed with the Δ12-desaturase gene from cyanobacterium

Potato (Solanum tuberosum L.) plants were transformed with the desA gene encoding Δ12 acyl-lipid desaturase in the cyanobacterium Synechocystis sp. PCC 6803. To evaluate the efficiency of this gene expression in the plant, its sequence was translationally fused with the sequence of the reporter gene encoding thermostable lichenase. A comparison of native and hybrid gene expression showed that lichenase retained its activity and thermostability within the hybrid protein, whereas desaturase retained its capability of inserting the double bond in fatty acid (FA) chains and, thus, to modify their composition in membrane lipids. In most transformed plants, shoots contained higher amounts of polyunsaturated FAs, linoleic and linolenic (by 39–73 and 12–41%, respectively). The total absolute content of unsaturated FAs was also higher in transformants by 20–42% as compared to wild-type plants. When transformed plants were severely cooled (to ?7°C), the rate of their membrane lipid peroxidation was not enhanced, whereas in wild-type plants, it increased substantially (by 25%) under such conditions. These results could indicate a higher tolerance of transformed plants to low temperatures and the oxidative stress induced by hypothermia.     

The Effect of Tobacco Plant Transformation with a Gene for Acyl-Lipid Δ9-Desaturase from <Emphasis Type="Italic">Synechococcus vulcanus</Emphasis> on Plant Chilling Tolerance

Tobacco plants with the introduced desC gene for acyl-lipid Δ9-desaturase from the thermophilic cyanobacterium Synechococcus vulcanus were cultivated on agar-solidified Murashige and Skoog nutrient medium supplemented with ferulic acid and antibiotics at 22°C and a 16-h photoperiod. Control plants were transformed with an empty pGA482 vector. The analysis of fatty acids (FAs) showed that, in transgenic plants, the level of 16:0 and 18:0 FAs decreased substantially, whereas the levels of di- and trienoic FAs increased. Transformed plants were more cold-tolerant. The tolerance to chilling was evaluated from electrolyte leakage from tissues damaged by cold treatments and from the accumulation of a product of lipid peroxidation, malondialdehyde. It was concluded that acyl-lipid Δ9-desaturase was actively expressed in transgenic tobacco plants and converted stearic acid into oleic acid, thus producing a substrate for further synthesis of di- and trienoic FAs. An increased proportion of polyunsaturated FAs in membrane lipids resulted in improved tobacco plant tolerance to chilling.     

The effect of radish plant rotation around a vertical axis on leaf morphology and lipid composition

The effects of radish (Raphanus sativus L. var. radicula D.C., cv. Rozovo-krasnyi s belym konchikom) plant clockwise rotation (2 rpm) around the vertical axis during their growth on growth parameters and also on the composition and content of polar lipids (PL) and neutral lipids (NL) in plant leaves and fatty acids included in them were studied. Rotation in the plane of the Earth’s magnetic field horizontal component did not affect the number of leaves but declined their total mass by 25%. However, a reduction of the root mass was observed only at elevated temperature. Rotation reduced the ratio of PL to NL by 1.5–2.0 times as compared with control, decreasing the content of such PL as glycoand phospholipids and increasing the content of NL, triacylglycerols and free sterols. Among glycolipids, the content of monogalactosyldiacylglycerols and digalactosyldiacylglycerols was reduced in comparison with stationary control, and among phospholipids the content of phosphatidylcholin was reduced by ∼50%. The ratio of phospholipids to sterols was reduced more than by 30%, indirectly indicating the decreased fluidity of the lipid bilayer of membranes; the ratio PL/NL was reduced as well, indicating an acceleration of age-dependent changes in the rotated plants. Rotation increased the content of linolenic acid and reduced the contents of oleic and erucic acids, thus increasing the index of fatty acid desaturation. We concluded that radish plant clockwise rotation around the vertical axis is an unfavorable factor inducing the appearance of the signs of premature senescence.     

The Effect of Helminthosporic Root Rot on the Composition of Acyl Lipids of Wheat Seedlings

The changes of the molecular species composition of esterified fatty acids (FAs) of total and nonextractable lipids were determined in roots and etiolated shoots of 3- to 10-day-old wheat (Triticum aestivumL.) seedlings infected with the fungus Bipolaris sorokiniana, the agent of helminthosporic root rot. A novel technique of assessing the extent of the infection-induced deviation of FA composition, mol %, from the control value was developed. It consists in the quantitative determination of both the deviations in this composition and the extent of contribution of separate FA species to the deviations observed. The application of this technique has shown that, for the total lipids, the maximum of such a deviation, in accordance with the membrane theory of stress, directly coincided in time with the onset of a decrease in the dry matter content in both roots and shoots. In each of these, the deviation was primarily caused by the change in the content of those FA species that usually dominate in a specific group of membrane lipids prevailing in a given organ, viz., plastid glycolipids in shoots and extraplastidal membrane phospholipids in roots. In both cases, C₂₀–C₂₂FAs significantly contributed to the deviations observed. This fact seems to reflect an enhanced formation of epicuticular waxes rich in these FAs on the shoot and root surfaces as an adaptive response of plants to fungal infection. Nonextractable (annular) membrane lipids, because of their vital importance for the survival of plant cells, differed from the total lipids with a far greater stability of their quantitative FA composition under conditions of infection-induced metabolic disturbances.     

Seasonal changes in the content of lipids,fatty acids,and pigments in brown alga <Emphasis Type="Italic">Costaria costata</Emphasis>

We compared the content of total lipids, particular lipid classes, fatty acids (FAs), and pigments in the brown alga Costaria costata [Turn.] Saund (Laminariaceae) in different seasons: during algal active life (April–June) and in the beginning of their degradation (the end of July). Lipids comprised only a small part of algal extractable compounds (10.9–13.4%). Their higher content was observed in May, in the period of alga preparation to sporogenesis. Polar lipids, glyceroglycolipids (GL) and phospholipids (PL), comprised a great part of total lipids in spring months and June, whereas neutral lipids, in July. GL predominated in polar lipids in spring and summer. The highest content of GL was observed in May and PL in April. Storage lipids comprised a great part of lipids in July. The content of sterols was the highest in April and May. In summer months, the content of sterols decreased and remained on unchanged level until thallus degradation. Polyunsaturated FAs (PUFAs) comprised the most part of FAs. Their highest content was observed in April. The main part of PUFAs were those of ω-3 series. In July, the level of these PUFA decreased substantially. The levels of ω-6 PUFAs were approximately similar in spring and summer months. The lowest content of monounsaturated FAs was detected in April; in May and July; the relative content of these FAs increased sharply. We also detected seasonal changes in the content of pigments and the ratios between them.     

Impact of weak permanent magnetic field on antioxidant enzyme activities in radish seedlings

The impact of weak permanent magnetic field (PMF) with magnetic flux density of 185–650 μT on activities of antioxidant enzymes in 5-day-old radish (Raphanus sativus L. var. radicula D.C.) seedlings, cv, Rosovo-krasnyi s belym konchikom was demonstrated. In the range of 185–325 μT PMF suppressed superoxide dismutase (SOD) activity in seedling grown in darkness and catalase (CAT) grown in both darkness and light. At the same values of magnetic flux density, all fractions of guaiacol peroxidase were activated and MDA accumulation was enhanced. At the higher values of magnetic flux density, SOD was activated in seedlings grown in darkness and CAT was activated in seedlings grown in both darkness and light. Other indices decreased or remained unchanged. It was concluded that PMF action depends on its intensity: at its low values, antioxidant enzymes are inhibited and at high values — activated.     

Substrate Specificity of Acyl-Lipid Δ9-Desaturase from <Emphasis Type="Italic">Cyanobacterium</Emphasis> sp. IPPAS B-1200, a Cyanobacterium with Unique Fatty Acid Composition

Cyanobacterium sp. IPPAS B-1200 is characterized by a high content of rare fatty acids (FAs), both myristic (14:0–30%) and myristoleic (14:1Δ9–10%) in the membrane lipids. Thus, short-chain FAs reach 40% of the sum of all FAs in cells, which is unusual for Cyanobacteria. Monounsaturated palmitoleic acids (16:1Δ9) also reach 40% of the sum of the FAs. We determined the complete nucleotide sequence of the genome of this cyanobacterium and found the only gene for the acyl-lipid Δ9-desaturase, desC1. We cloned this gene and characterized its specificity to the length of the substrate using heterologous expression in Escheriсhia coli. The results show that DesC1 nonspecifically generates olefin bond in FAs with a length of 14, 16, and 18 carbon atoms. This finding confirms that all monoesterifed FAs in Cyanobacterium sp. IPPAS B-1200 are generated by one acyl-lipid Δ9-desaturase.