Effect of different extenders on glutamic oxalacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) release from frozen buffalo semen

Sixty buffalo semen samples (motility greater than 60%) were frozen in 3 extenders, viz., Tris yolk glycerol (TY-G), Citric acid whey glycerol (CAW-G) and Egg yolk glucose sodium bicarbonate glycerol (EYGSB-G) for studying the release of GOT and GPT enzymes in the extracellular fluid during pre-freezing (after first extension) and post-freezing (15 minutes and 30 days after freezing). Release of GOT and GPT enzymes was less in TY-G than CAW-G and EYGSB-G extenders. Significant differences (P<0.01) in GOT and GPT release were observed between extenders and bulls at various stages of freezing of semen.     

Soluble glutamic oxalacetic transaminase (GOT) polymorphism in cattle

Zymograms performed on extracts of bovine white cells revealed two phenotypes in the soluble (anodal) isozyme of glutamic oxalacetic transaminase (GOT). Genetic data are consistent with the hypothesis that two co-dominant, autosomal alleles, GOT-1-A and GOT-1-B , give rise to the phenotypes GOT-1-AA, GOT-1-AB and GOT-1-BB. The phenotype BB has not been encountered. The allele GOT-1-B has been observed in one of the five breeds surveyed, the Central and Upper Belgium breed where it has a frequency of about 0,008.