共查询到20条相似文献,搜索用时 140 毫秒
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2.
H.J. Clarke J.G. Wilson I. Kuo M.M. Lülsdorf N. Mallikarjuna J. Kuo K.H.M. Siddique 《Plant Cell, Tissue and Organ Culture》2006,85(2):197-204
The main constraint to the transfer of desired traits into cultivated chickpea from wild Cicer relatives is the presence of post-zygotic barriers which result in abortion of the immature embryo following interspecific hybridisation. Rescue of hybrid embryos in vitro and regeneration of hybrid plantlets could allow chickpea breeders to transfer desirable traits from wild relatives of chickpea. The development of embryo rescue techniques using selfed chickpea and selfed wild relatives is being used as a first step to protocols for wide hybrids. Optical microscopy studies of embryogenesis, in both selfs and hybrids, identified deleterious changes in the fertilised hybrid seed as early as 2–4 days after pollination in some crosses. These observations suggest that the appropriate time to rescue chickpea × C. bijugum hybrids is at the early globular stage of embryogenesis (2–7 days old), which requires the development of a complex tissue culture medium. In contrast hybrids between chickpea × C. pinnatifidum abort later (up to 15–20 days old) at the heart-shaped or torpedo stages, and are easier to rescue in vitro. Genotype also plays a significant role in the ability of immature selfed ovules to germinate in vitro. In this paper we report on the optimisation of␣protocols for rescueing immature embryos using selfed chickpea and its wild relatives in ovule, and subsequently to regenerate plantlets. 相似文献
3.
M. Ramsey 《Oecologia》1995,103(1):101-108
The extent, frequency and causes of pollenlimited seed production were examined in partially selffertile populations of Blandfordia grandiflora for 2 years. Percentage seed set of open-pollinated plants (50–57%) did not differ within or between years, and was about 19% less than experimentally cross-pollinated plants (70–75%). Floral visits by honeybees did not differ through the flowering season and the number of pollen grains deposited on stigmas within 12 h of flowers opening exceeded the number of ovules per flower, indicating that the quality rather than the quantity of pollination limited seed set. Pollen limitation was caused by concurrent self- and cross-pollination and the subsequent abortion of some selfed ovules due to inbreeding depression. Natural seed set (55%) was intermediate between selfed (43%) and crossed (75%) flowers and was not increased when flowers that had been available to pollinators for 24 h were hand cross-pollinated, suggesting that ovules were already fertilized. Similarly, experimental pollination with both cross and self pollen within 24 h of flowers opening did not increase seed set relative to natural seed set, indicating that both cross- and self-fertilizations had occurred. In contrast, when selfing followed crossing by 48 h, or vice versa, seed set did not differ from crossed-only or selfed-only flowers, respectively, indicating that ovules were pre-empted by the first pollination. Collectively, these results indicate that under natural conditions self pollen pre-empts ovules, rendering them unavailable for cross-fertilization. This selfing reduces fecundity by 50%, as estimated from the natural production of cross seeds when selfing was prevented. Consequently, selection should favour floral traits, such as increased stigma-anther separation or protandry, that reduce interference between male and female functions that leads to selfing. 相似文献
4.
M. O. Duarte C. Mendes‐Rodrigues M. F. Alves P. E. Oliveira D. S. Sampaio 《Plant biology (Stuttgart, Germany)》2017,19(2):140-146
- Mixed cross and self‐pollen load on the stigma (mixed pollination) of species with late‐acting self‐incompatibility system (LSI) can lead to self‐fertilized seed production. This “cryptic self‐fertility” may allow selfed seedling development in species otherwise largely self‐sterile. Our aims were to check if mixed pollinations would lead to fruit set in LSI Adenocalymma peregrinum, and test for evidence of early‐acting inbreeding depression in putative selfed seeds from mixed pollinations.
- Experimental pollinations were carried out in a natural population. Fruit and seed set from self‐, cross and mixed pollinations were analysed. Further germination tests were carried out for the seeds obtained from treatments.
- Our results confirm self‐incompatibility, and fruit set from cross‐pollinations was three‐fold that from mixed pollinations. This low fruit set in mixed pollinations is most likely due to a greater number of self‐ than cross‐fertilized ovules, which promotes LSI action and pistil abortion. Likewise, higher percentage of empty seeds in surviving fruits from mixed pollinations compared with cross‐pollinations is probably due to ovule discounting caused by self‐fertilization. Moreover, germinability of seeds with developed embryos was lower in fruits from mixed than from cross‐pollinations, and the non‐viable seeds from mixed pollinations showed one‐third of the mass of those from cross‐pollinations.
- The great number of empty seeds, lower germinability, lower mass of non‐viable seeds, and higher variation in seed mass distribution in mixed pollinations, strongly suggests early‐acing inbreeding depression in putative selfed seeds. In this sense, LSI and inbreeding depression acting together probably constrain self‐fertilized seedling establishment in A. peregrinum.
5.
T. B. H. Reusch 《Journal of evolutionary biology》2001,14(1):129-138
Plant mating systems have received considerable attention because the proportion of selfed vs. outcrossed progeny is an important evolutionary factor. In clonally reproducing plants, geitonogamous selfing between distant ramets belonging to the same genet is expected to be widespread, yet empirical data are sparse. Nothing is known about between‐ramet selfing in aquatic flowering plants with subaqueous pollen transfer, most of which display pronounced clonal reproduction. From two locations in the western Baltic Sea, I present data on the effects of patch isolation and clonal diversity on the outcrossing rate of eelgrass, Zostera marina L., based on the genotypes of maternal plants and recently fertilized ovules scored at eight microsatellite loci. There were no differences in outcrossing rates between vegetation patches and continuous meadow although patches were nearly always composed of single genets. Quantitative effects of clonal diversity were present in the continuous vegetation where a significant positive correlation between genet diversity and the proportion of outcrossed offspring was detected (Kendall’s τ=0.82, P=0.0017). On a population‐scale as well, the genotypic diversity was positively correlated with outcrossing. The relative fitness of selfed offspring was low (ω ± 95% confidence interval=0.56 ± 0.032 and 0.322 ± 0.15) indicating that geitonogamy incurred substantial fitness costs. Selfing rates in Z. marina may not be in evolutionary equilibrium because of spatial and temporal heterogeneity of clonal size and diversity. The high prevalence of dioecy in seagrasses may have evolved to avoid the fitness costs associated with geitonogamy. 相似文献
6.
Keith Karoly 《American journal of botany》1994,81(12):1538-1544
The consequences of inbreeding for reproductive traits were investigated for two closely related annual lupines that differ in their mating system. Lupinus bicolor (Leguminosae) is a primarily selling species while Lupinus nanus outcrosses at intermediate rates. A controlled crossing program was used for each species to produce selfed and outcrossed progeny. These progeny were then grown in a greenhouse and scored for the date of first flower, flower morphology, and autofertility. Selfed progeny of L. bicolor produced significantly smaller flowers but did not differ from outcrossed progeny for the remaining traits. Selfed progeny of L. nanus produced flowers that significantly differed in shape and had fewer ovules than the flowers of outcrossed progeny. Selfed progeny of L. nanus also had significantly lower rates of autofertility in comparison to outcrossed progeny. The significant effects of inbreeding on these mating system traits may indicate the presence of directional dominance at the loci underlying these characters. The consequences of these direct effects of inbreeding on reproductive traits for plants growing in natural populations may include nonadaptive changes in the outcrossing rate between generations. 相似文献
7.
Nelson Sabino Bittencourt Jr. Camila Ismael Giorgi Moraes 《Plant Systematics and Evolution》2010,288(1-2):59-76
Handroanthus chrysotrichus shows pollination-dependent self-fertility, polyploidy, and adventitious polyembryony, and it is closely related to H. ochraceus, for which apparently conflicting reports of self-incompatibility and apomixis have been published. The present study aims to investigate the polyembryony in these species by means of histological analysis of ovule/seed development in unpollinated, selfed, and crossed pistils/fruits (in H. chrysotrichus only) as well as seed germination experiments. Experimental pollinations were carried out to evaluate breeding systems in the studied populations, and the results indicated self-fertility in both species. Adventitious embryo precursor cells (AEPs) were formed in the ovules of unpollinated, selfed, and crossed pistils. However, unfertilized ovules never develop into seeds, and fertilization/endosperm initiation clearly stimulates the formation of AEPs in pollinated pistils. The inability of AEP-bearing unfertilized ovules to initiate endospermogenesis clearly shows that fertilization is needed for adventitious embryo development. Consequently, formation of AEPs is required but is not sufficient for apomictic reproduction in H. chrysotrichus. Analysis of the positions of multiple embryos in the endosperm indicated that fertilized ovules are able to develop into seeds even in the absence of a zygotic embryo. The development of AEPs in ovules of H. chrysotrichus foregoes the stage in which activation of selfed pistil rejection takes place in H. impetiginosus, a species with late-acting self-incompatibility. Our study supports the hypothesis that the self-fertility in H. chrysotrichus (and perhaps also in H. ochraceus) resulted from the emergence of pseudogamous apomixis, favored by the physiological peculiarities of the late-acting self-incompatibility and possibly related to polyploidy. 相似文献
8.
In plants, selfing and outcrossing may be affected by maternal mate choice and competition among pollen and zygotes. To evaluate this in Silene nutans, we pollinated plants with mixtures of (1) self‐ and outcross pollen and (2) pollen from within a population and from another population. Pollen fitness and zygote survival was estimated from the zygote survival and paternity of seeds. Self pollen had a lower fitness than outcross pollen, and selfed zygotes were less likely, or as likely, to develop into seeds. Hybrid zygotes survived as frequently or more than local zygotes, and pollen from one of the populations fertilized most ovules in both populations. Our results thus indicate strong maternal discrimination against selfing, whereas the success of outbreeding seems mostly affected by divergent pollen performance. The implications for the evolution of maternal mate choice are discussed. 相似文献
9.
In gynodioecious species, females contribute genes to future generations only through ovules, and to persist in populations
they must have a compensatory advantage compared with hermaphrodites that reproduce via ovules and pollen. This compensation
can result from greater fecundity and/or superior success of progeny from females. We examined differences in seed production
and progeny success between females and hermaphrodites in the geophyte Wurmbea biglandulosa to explain the maintenance of females. Females produced more ovuliferous flowers and had more ovules per flower than did
hermaphrodites but this did not necessarily result in greater fecundity, in part because seed production of females was pollen-limited.
Over four years in one population, open-pollinated females produced 1.32 more seeds than open-pollinated hermaphrodites (range
1.09–1.63). In two other populations examined for one year only females produced 1.07 and 0.79 as many seeds as hermaphrodites.
Seed production of open-pollinated females and hermaphrodites was only 55% and 73% that of cross-pollinated plants, respectively,
indicating that both genders were pollen-limited but females more so than hermaphrodites. Open-pollinated seeds from females
were 1.18–1.27 times more likely to germinate than seeds from hermaphrodites. No gender differences existed in seedling growth
or survival. Hermaphrodites were self-compatible, but selfed seed set was only 80% that of crossed seed set. Crossed seed
set of females and hermaphrodites did not differ. Assuming nuclear control of male sterility, relative female fitness is insufficient
to maintain females at their current frequencies of 17%, and substantial female fitness advantages at later life-cycle stages
are required.
Received May 4, 2001 Accepted February 25, 2002 相似文献
10.
SANDY‐LYNN STEENHUISEN STEVEN D. JOHNSON 《Botanical journal of the Linnean Society. Linnean Society of London》2012,169(3):433-446
It has been assumed that species of the large African genus Protea have strong self‐incompatibility systems. However, this assumption was based largely on studies conducted on a clade of bird‐pollinated species that occur in the shrubby fynbos vegetation of the Cape region of southern Africa. To test whether self‐incompatibility occurs in a grassland/savanna Protea clade, which is largely insect‐pollinated, we performed controlled pollination experiments on four species, P. caffra, P. dracomontana, P. simplex and P. welwitschii. Although pollen–ovule ratios of all four species fall within the range for outcrossers, all four species are self‐compatible and capable of autonomous seed production. Using fluorescence microscopy, we found that self‐pollen tubes had the same probability of reaching ovules as cross‐pollen tubes. In the small tree P. caffra, selfed progeny had rates of germination and survivorship that were identical to those of crossed progeny. The grassland Protea spp. studied are likely to have mixed mating systems on account of being both visited by insects and capable of autonomous selfing. If one assumes previous reports of self‐incompatibility in Protea to be reliable, there have been at least five losses of self‐incompatibility and two gains of autonomous selfing in this genus. However, earlier studies in the genus were often methodologically flawed and a thorough re‐analysis of breeding systems in Protea is required. © 2012 The Linnean Society of London, Botanical Journal of the Linnean Society, 2012, 169 , 433–446. 相似文献
11.
We investigated the fates of ovules following self- and cross-pollination of the variably self-sterile Epilobium obcordatum by observing thin sections of ovaries 4, 5, and 10 d postpollination (PP). Three types of unfertilized ovules were observed: apparently normal ovules not entered by pollen tubes, nonfunctional ovules with a nucellus but lacking an embryo sac, and empty ovules with integuments but lacking a nucellus. The average frequencies for all three types were not different for selfs and crosses. Fertilization frequencies did not differ among pollination treatments, averaging 61.4% of the total sample of 4 and 5 d PP ovules. The phenotypes of failing postfertilization ovules at 4 and 5 d PP included those containing preglobular and globular embryos lacking endosperm, ovules with endosperm but no embryo present, and failing embryos in the presence of endosperm. Self- and cross-fertilized 10 d PP ovules also possessed heart-stage and torpedo-stage failing embryos. Early embryo failures (5 d PP) were distributed throughout the length of the ovary, as expected if failures are the consequence of factors intrinsic to each ovule. The sizes of normally developing self- and cross-fertilized embryos were not different at 4 d PP. The 5 d PP cross embryos were larger than selfs. A comparison of 10 d samples was not possible due to attrition of self embryos. Although ovular position did not affect the probability of early embryo failure, it did affect the size of normally developing embryos at 4, 5, and 10 d PP, with embryos at the stylar end larger than those at the basal end. 相似文献
12.
Consequences of interspecific hybridization and virus infection on the growth and fecundity of three threatened coastal Lepidium (Brassicaceae) species from New Zealand
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Josh C. C. M. Van Vianen Gary J. Houliston John D. Fletcher Peter B. Heenan Hazel M. Chapman 《Austral ecology》2015,40(6):672-682
Lepidium castellanum, L. juvencum and L. oleraceum are threatened coastal cresses endemic to New Zealand. These three species were selfed and interspecific hybrids generated for examination of hybrid fitness and inbreeding depression. In controlled glasshouse experiments, the interspecific hybrids and selfed progeny were inoculated with a strain of the introduced Turnip mosaic virus (TuMV) previously isolated from wild populations of L. aegrum. Experiments tested the hypothesis that heterosis in the interspecific hybrids provides a gain in TuMV resistance in comparison to selfed plants. We show that interspecific hybrids of three genetically distinct species of Lepidium increased plant performance and reduced susceptibility to the effects of the TuMV. We suggest that interspecific hybridization could be implemented as a conservation management strategy and that a broader outlook may be required to mitigate the negative impacts of introduced pathogens on threatened species. 相似文献
13.
Selfing and inbreeding depression in seeds and seedlings of Neobalanocarpus heimii (Dipterocarpaceae) 总被引:1,自引:0,他引:1
Naito Y Konuma A Iwata H Suyama Y Seiwa K Okuda T Lee SL Muhammad N Tsumura Y 《Journal of plant research》2005,118(6):423-430
We evaluated the degree of selfing and inbreeding depression at the seed and seedling stages of a threatened tropical canopy tree, Neobalanocarpus heimii, using microsatellite markers. Selection resulted in an overall decrease in the level of surviving selfed progeny from seeds to established seedlings, indicating inbreeding depression during seedling establishment. Mean seed mass of selfed progeny was lower than that of outcrossed progeny. Since the smaller seeds suffered a fitness disadvantage at germination in N. heimii, the reduced seed mass of selfed progeny would be one of the determinants of the observed inbreeding depression during seedling establishment. High selfing rates in some mother trees could be attributed to low local densities of reproductive individuals, thus maintenance of a sufficiently high density of mature N. heimii should facilitate regeneration and conservation of the species. 相似文献
14.
Greenhouse pollinations were performed to determine whether early-acting inbreeding depression is contributing to low levels
of self-fertility in three Vaccinium species: V. myrtilloides Michaux, V. angustifolium Aiton, and V. corymbosum L. All three species showed a significant reduction in self fruit set and in the proportion of fertilized ovules that developed
into mature seed in self compared to outcross fruit. Reductions were more severe in V. myrtilloides and V. angustifolium than in V. corymbosum; however, early-acting inbreeding depression appeared to be the primary factor limiting self-fertility in all three species.
Evidence for early- acting inbreeding depression included the presence and higher proportion of aborted ovules in self fruit
than in outcross fruit, a correlation between levels of self and outcross seed set, and pollen chase experiments demonstrating
that self-pollen does fertilize the ovules. Self-fertility in the three species was probably influenced by levels of genetic
load, ploidy level and outcrossing rates.
Received: 10 December 1999 / Revision accepted: 20 June 2000 相似文献
15.
The interaction of mating system and nutrient limitation in determining seed production was investigated in the annual, self-compatible plant Lupinus texensis (Fabaceae). Abortion of developing seeds is a major factor limiting seed production in natural populations (17-28%). Selfing rates are generally low (0.02-0.21), suggesting that deleterious recessive genes may be maintained at significant levels in natural populations. The average inbreeding depression associated with seed development is δ = 0.24. Nutrient limitation reduced seed output across experimental treatments by a factor of 0.22 through decreased production of inflorescences, flowers, and ovules, and by a factor of 0.29 through increased abortion of fruits and of seeds within fruits. Competition for resources among fruits increased the frequency of seed abortion. Moreover, a greater proportion of selfed seeds were aborted as the overall abortion rate increased. Estimates of genetic load may therefore only be appropriate if undertaken in the field, and inbreeding depression may vary from year to year simply due to changes in environmental conditions rather than to underlying genetic changes in populations. The existence of inbreeding depression and the high frequency of abortions suggest that selective abortion favoring outcrossed progeny occurs in natural populations of L. texensis. 相似文献
16.
Arlee M. Montalvo 《Evolution; international journal of organic evolution》1992,46(4):1181-1198
Flowers frequently receive both self (S) and outcross (OC) pollen, but S pollen often sires proportionally fewer seeds. Failure of S pollen can reflect evolved mechanisms that promote outcrossing and/or inbreeding depression expressed during seed development. The relative importance of these two processes was investigated in Aquilegia caerulea, a self-compatible perennial herb. In the field I performed single-donor (S or OC) and mixed-donor (S plus OC) pollinations to compare the relative success of both pollen types at various stages from pollen germination to seed maturity. Single-donor S pollinations produced significantly fewer and lighter seeds (x decrease = 12% and 3%, respectively) than OC pollinations. Abortion rates differed by an average of 38% whereas fertilization rates differed by only 5%, indicating that most differences in seed number arose postzygotically. This suggests that inbreeding depression was responsible for most failure of S pollen. One prezygotic effect measured was that 10% fewer S than OC pollen tubes reached ovaries after 42 hr, suggesting S pollen might fertilize proportionately fewer ovules after mixed pollination. Using allozyme markers, I found mixed-donor pollinations produced significantly more and heavier outcrossed than selfed seeds. However, the proportion of selfed seed, fertilized ovules, and aborted seeds for mixed-donor fruits were each predictable from pollen performance in single-donor fruits, suggesting that differential paternity is best explained by inbreeding depression during seed development. Even given these similarities between mixed- and single-donor fruits in the relative performance of S and OC pollen, both individual seed weight and seed set were significantly higher in multiply-sired fruits. 相似文献
17.
H. Lloyd Mogensen 《American journal of botany》1975,62(2):160-165
This study deals with the occurrence and relative abundance of four different types of abortive ovules in three species of Quercus. It was found that, contrary to previous literature, fertilization does not always occur in the abortive ovules. The most common type of abortive ovule is the one in which a normal embryo sac develops, yet fertilization does not occur. The absence of embryo sacs and the occurrence of empty embryo sacs account for abortion in other ovules. All types of abortive ovules can occur in the same ovary. It is proposed that all of the ovules that develop a normal embryo sac are potential seeds, but the first one to be fertilized suppresses the normal development of the others. 相似文献
18.
The reproductive biology of Zeyheria montana was studied through field observations of flower visitors and floral events, controlled manual pollinations, and observations of pollen tube growth and ovule penetration by fluorescence microscopy. Analysis of secretory areas of the nectar chamber was made by flower dissections and histology of serial sections. The flower lasted 6–8 days, but pollen exposure and stigma receptivity occurred only up to the end of the first and second days, respectively. Pollination was effected by several species of hummingbirds, especially Colibri serrirostris. The flowers present a rudimentary, non-functional disc, and secretion of nectar is performed by corolla-borne glandular trichomes. Only hand cross-pollinated and natural-pollinated flowers set fruits. Artificially self-pollinated and non-pollinated flowers dried off after anthesis without presenting any swelling of the ovary. Almost all the ovules in selfed and crossed pistils were penetrated 96 h after pollination. However, a delay in ovule penetration in self-pollinated pistils was verified, which indicates the occurrence of late-acting self-incompatibility.We are grateful to Fundo de Apoio ao Ensino e à Pesquisa (UNICAMP) for financial support, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the fellowship granted to the first author (this work is part of a Ph.D. thesis presented to UNICAMP, Campinas, SP, Brazil). 相似文献
19.
Reduced seed yields following self-pollination have repeatedly been observed, but the underlying mechanisms remain elusive when self-pollen tubes can readily grow into ovaries, because pre-, post-zygotic late-acting self-incompatibility (LSI), or early-acting inbreeding depression (ID) can induce self-sterility. The main objective of this study was to differentiate these processes in Aconitum kusnezoffii, a plant lacking stigmatic or stylar inhibition of self-pollination. We performed a hand-pollination experiment in a natural population of A. kusnezoffii, compared seed set among five pollination treatments, and evaluated the distribution of seed size and seed set. Embryonic development suggested fertilization following self-pollination. A partial pre-zygotic LSI was suggested to account for the reduced seed set by two lines of evidence. The seed set of chase-pollination treatment significantly exceeded that of self-pollination treatment, and the proportion of unfertilized ovules was the highest following self-pollination. Meanwhile, early-acting ID, rather than post-zygotic LSI, was suggested by the findings that the size of aborted selfed seeds varied continuously and widely; and the selfed seed set both exhibited a continuous distribution and positively correlated with the crossed seed set. These results indicated that the embryos were aborted at different stages due to the expression of many deleterious alleles throughout the genome during seed maturation. No signature of post-zygotic LSI was found. Both partial pre-zygotic LSI and early-acting ID contribute to the reduction in selfed seed set in A. kusnezoffii, with pre-zygotic LSI rejecting part of the self-pollen and early-acting ID aborting part of the self-fertilized seeds. 相似文献
20.
Hoa Tran Hon g Vu Aussie Mahunu David Chien Joseph Arditti Robert Ernst 《American journal of botany》1995,82(9):1089-1094
Thickening of perianth segments, loss of original nonchlorophyllous pigments, and chlorophyll production occur in Phalaenopsis species of section Zebrinae Pfitzer as postpollination phenomena. This also occurs in several hybrids of members of this section with Phalaenopsis species whose perianth wilts following pollination. The observed dilution of pigments in hybrids containing regreening and wilting genomes indicates involvement of dominant nuclear genes. Increases in chlorophyll levels are most pronounced within 40 d following pollination and increase moderately or level off after 80 d. Seed capsules and perianths of selfed species, which had not dehisced, turned yellowish 160 d after pollination and had reduced levels of chlorophyll a, but not chlorophyll b. 相似文献