共查询到20条相似文献,搜索用时 15 毫秒
1.
G. Charmet G. Branlard 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,71(2):193-200
Summary Sixty single seed descent (SSD) lines and about 25 anther-derived doubled-haploid (DH) lines were obtained from two triticale crosses. The frequency distributions of 10 quantitative agronomic traits were compared using parametric and non-parametric tests. A multivariate discriminant analysis was subsequently carried out. Gliadin patterns obtained from each line by polyacrylamide gel electrophoresis were used to calculate intra- and inter-population diversities from relative dissimilarity indices. It was found that DH and SSD lines show significant differences in frequency distributions of 1000 grain weight in both crosses, of heading date for one cross, and of lodging susceptibility for the other cross. The results of intra- and inter-population gliadin diversity indicate that although the SSD method theoretically provides more opportunity for recombination to occur than the DH method, it did not produce a greater range of recombinants. Since there is no significant difference between SSD- and DH-line distributions for grain yield, anther culture appears to be an efficient method for producing high yielding homozygous lines from F1 hybrids of triticale in a relatively short time. 相似文献
2.
The paper presents a proposition for detection of linkage of genes responsible for metrical traits. Taking into account the expected means for early generations (F(1), F(2), F(3)) and a population of homozygous lines (in this case doubled haploid lines, DH derived from a cross between two homozygous parents) as well as estimators of genetic parameters m, [d], [i], [h] and [l], the expected values for these parameters in the presence of linkage have been formulated. It was found that when there is no linkage, the expression F(1) - 6F(2) + 8F(3) - 3DH(mean) is equal to zero. Thus, an experiment covering DH lines and F(1), F(2), F(3) hybrids makes it possible to obtain, beside information of interest, also information on presence or absence of linkage. 相似文献
3.
Guo S Zou J Li R Long Y Chen S Meng J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,125(6):1113-1124
Brassica carinata is an important oilseed crop with unique favourable traits that are desirable for other Brassica crops. However, given the limited research into genetic resources in B. carinata, knowledge of the genetic structure of this species is relatively poor. Nine homozygous, genetically distinct accessions of B. carinata were obtained via microspore culture, from which two divergent doubled haploid (DH) lines were used to develop a DH mapping population that consisted of 183 lines. The mapping population showed segregation of multiple traits of interest. A genetic map was constructed with PCR-based markers, and a total of 212 loci, which covered 1,703?cM, were assigned to eight linkage groups in the B genome and nine linkage groups in the C genome, which allowed comparison with genetic maps of other important Brassica species that contain the B/C genome(s). Loci for two Mendelian-inherited traits related to pigmentation (petal and anther tip colour) and one quantitative trait (seed coat colour) were identified using the linkage map. The significance of the mapping population in the context of genetic improvement of Brassica crops is discussed. 相似文献
4.
T. J. Riggs J. W. Snape 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1977,49(3):111-115
Summary Comparisons were made between the genetic means and variances of a quantitative trait determined by 8 loci in simulated populations of lines derived by diploidizing haploids (DH) on the one hand and by single seed descent (SSD) on the other.In the absence of linkage no differences between the populations were observed, but when linkage was present, recombination was more frequent in the SSD populations as indicated by the relative differences in variance between these and the DH populations. In addition, differences in means between the populations derived by the two methods were observed when non-allelic interaction was present. The direction and magnitude of the differences in both means and variances depended upon the linkage phase, the recombination frequency and the presence or absence of interaction.The conclusion was drawn that the SSD method was to be preferred from theoretical considerations although in practice the choice of method will also depend upon practical and technical factors. 相似文献
5.
6.
T. M. Choo E. Reinbergs S. J. Park 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1982,61(3):215-218
Summary Both doubled haploid (DH) and single seed descent (SSD) methods were used to derive homozygous lines from two crosses of barley. The frequency distributions of grain yield, heading date, and plant height of the DH and SSD lines were compared by the Mann-Whitney U test, Kolmogorov-Smirnov twosample test and Wald-Wolfowitz runs test. It was found that the DH lines distributed in the same manner as the SSD lines with respect to the three characters. The results indicated that although the SSD method had more opportunity for recombination than the DH method, it did not produce a sample of recombinants which differed significantly from the DH sample; thus both methods were equally efficient for use in deriving homozygous lines from F1 hybrids in a relatively short time.Contribution no. 455 Charlottetown Research Station, Agriculture Canada, P.E.I. (Canada) 相似文献
7.
利用DH或RIL群体检测QTL体系并估计其遗传效应 总被引:39,自引:1,他引:38
利用DH和RIKL群体并结合重复内分组随机区组设计对和物产量等遗传率较低的数量性状进行分离分析可提高遗传分析的精度。根据混合分布理论菜了利用DH或RIL群体重复实验数据鉴定数量性状混合遗传模型的分离分析法,特别是2对链锁主基因+多基因模型。该方法可鉴定数量性状的遗传模型和主基因的作用方式,估计主基因、多基因的遗传疚和遗传方差,在两主基因存在连锁可可估计其重组率。下面通过应用举例说明该方法。 相似文献
8.
9.
Walley PG Carder J Skipper E Mathas E Lynn J Pink D Buchanan-Wollaston V 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(3):467-484
A unique broccoli × broccoli doubled haploid (DH) population has been created from the F1 of a cross between two DH broccoli lines derived from cultivars Green Duke and Marathon. We genotyped 154 individuals from
this population with simple sequence repeat and amplified fragment length polymorphism markers to create a B. oleracea L. var. italica ‘intra-crop’ specific framework linkage map. The map is composed of nine linkage groups with a total length of 946.7 cM.
Previous published B. oleracea maps have been constructed using diverse crosses between morphotypes of B. oleracea; this map therefore represents a useful breeding resource for the dissection of broccoli specific traits. Phenotype data
have been collected from the population over five growing seasons; the framework linkage map has been used to locate quantitative
trait loci for agronomically important broccoli traits including head weight (saleable yield), head diameter, stalk diameter,
weight loss and relative weight loss during storage, as well as traits for broccoli leaf architecture. This population and
associated linkage map will aid breeders to directly map agronomically important traits for the improvement of elite broccoli
cultivars. 相似文献
10.
Chen W Zhang Y Liu X Chen B Tu J Tingdong F 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(6):849-858
The inheritance of yield-related traits in rapeseed (Brassica napus) is poorly understood, and the investigations on mapping of quantitative trait loci (QTL) for such traits are only few. QTL
related to six traits were mapped which include plant height (PH), height of lowest primary effective branch (HPB), length
of main inflorescence (LMI), silique length (SL), number of primary branches (FB) and silique density (SD). A set of 258 doubled
haploid (DH) lines derivatives of a cross between a canola variety Quantum and a resynthesized B. napus line No.2127-17, and a fixed immortalized F2 (designated as IF2) population generated by randomly permutated intermating of these DHs were investigated. A genetic linkage map was constructed
using 208 SSR and 189 SRAP markers for the DH population. Phenotypic data were collected from three environments for the two
populations. Using composite interval mapping analyses, 30 and 22 significant QTL were repeatedly detected across environments
for the six traits in the DH and IF2 populations, respectively. Twenty-nine QTL were common between the two populations. The directions of parental contribution
for all common QTL were the same, showing a great potential for marker-assisted selection in improving these traits. Some
chromosomal regions harbor QTL for multiple traits, which were consistent with significant phenotypic correlations observed
among traits. The results provided a better understanding of the genetic factors controlling yield-related traits in rapeseed.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
11.
Linkage Map Construction and Quantitative Trait Loci Analysis for Bolting Based on a Double Haploid Population of Brassica rapa 总被引:7,自引:0,他引:7
Xu Yang Yang-Jun Yu Feng-Lan Zhang Zhi-Rong Zou Xiu-Yun Zhao De-Shuang Zhang Jia-Bing Xu 《植物学报(英文版)》2007,49(5):664-671
Early bolting of Chinese cabbage (Brassica rapa L.) during spring cultivation often has detrimental effects on the yield and quality of the harvested products. Breeding late bolting varieties is a major objective of Chinese cabbage breeding programs. In order to analyze the genetic basis of bolting traits, a genetic map of B. rapa was constructed based on amplified fragment-length poiymorphism (AFLP), sequence-related amplified poiymorphism (SRAP), simple sequence repeat (SSR), random amplification of polymorphic DNA (RAPD), and isozyme markers. Marker analysis was carried out on 81 double haploid (DH) lines obtained by microspore culture from F1 progeny of two homozygous parents: B. rapa L. ssp. pekinensis (BY) (an extra-early bolting Chinese cabbage line) and B. rapa L. ssp. rapifera (MM) (an extra-late bolting European turnip line). A total of 326 markers including 130 AFLPs, 123 SRAPs, 16 SSRs, 43 RAPDs and 14 isozymes were used to construct a linkage map with 10 linkage groups covering 882 cM with an average distance of 2.71 cM between loci. The bolting trait of each DH line was evaluated by the bolting index under controlled conditions. Quantitative trait loci (QTL) analysis was conducted using multiple QTL model mapping with MapQTL5.0 software. Eight QTLs controlling bolting resistance were identified. These QTLs, accounting for 14.1% to 25.2% of the phenotypic variation with positive additive effects, were distributed into three linkage groups. These results provide useful information for molecular marker-assisted selection of late bolting traits in Chinese cabbage breeding programs. 相似文献
12.
Ariana Valdés Rosemarie Clemens Christian Möllers 《Molecular breeding : new strategies in plant improvement》2018,38(5):65
Oilseed rape (Brassica napus L.) is among dicotyledonous plants, a model species for microspore embryogenesis. Tremendous differences exist among oilseed rape genotypes in their embryogenic response and direct embryo to plant conversion. Despite some attempts to identify relevant genes, the genetic basis of these traits remains largely unknown. The objective of this work was to develop and to provide to the scientific community a doubled haploid (DH) population derived from a cross of the reported highly embryogenic genotype DH4079 and the low embryogenic inbred line Express 617. A population of 198 DH-lines was generated and genotyped with the Brassica 60 K Illumina Infinium? SNP array. The parental and the F1 genotypes as well as between 81 and 107 DH-lines were characterized for their number of microspores, number of microspore-derived embryos, embryo survival rate, direct embryo to shoot conversion, and related traits. The results obtained for the F1 genotype were mostly in between the two parents. SNP markers in the DH population showed to 49% distorted segregation and of those 63% were in favor of DH4079. Significant genotypic differences were found for all traits and heritabilities ranged from 66 to 88%. Together, 13 quantitative trait loci (QTL) for the different traits were identified on linkage groups A01, A02, A05, A10, C04, and C06, and candidate genes were identified within their QTL confidence intervals. 相似文献
13.
利用双单倍体群体剖析水稻产量及其相关性状的遗传基础 总被引:23,自引:0,他引:23
主效QTL、上位性效应和它们与环境的互作(QE)都是数量性状的重要遗传因素。利用籼粳交珍汕97/武育粳2号F1植株上的花药进行组织培养得到的190个双单倍体群体和179个微卫星标记,通过两年两重复田间试验,采用混合线性模型方法分析了9个控制水稻产量及其相关性状的遗传效应,得到57个主效QTL,41对上位性互作,8对QTL与环境的互作和7对上位性效应与环境的互作。单个主效QTL解释这些性状1.3%~25.8%的表型方差。各性状QTL的累积表型贡献率达11.5%~66.8%。大多数性状之间具有显著的表型相关性,相关性较高的性状之间常具有较多共同或紧密连锁的QTL。结果表明,基因的多效性或紧密连锁可能是性状相关的重要遗传基础。 相似文献
14.
Ramchiary N Padmaja KL Sharma S Gupta V Sodhi YS Mukhopadhyay A Arumugam N Pental D Pradhan AK 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(6):807-817
Quantitative trait loci (QTL) analysis of yield influencing traits was carried out in Brassica juncea (AABB) using a doubled haploid (DH) mapping population of 123 lines derived from a cross between Varuna (a line representing
the Indian gene pool) and Heera (representing the east European gene pool) to identify potentially useful alleles from both
the parents. The existing AFLP based map of B. juncea was further saturated with RFLP and SSR markers which led to the identification of the linkage groups belonging to the A
(B. rapa) and B (B. nigra) genome components of B. juncea. For QTL dissection, the DH lines were evaluated at three different environments and phenotyped for 12 quantitative traits.
A total of 65 QTL spread over 13 linkage groups (LG) were identified from the three environments. QTL analysis showed that
the A genome has contributed more than the B genome to productivity (68% of the total QTL detected) suggesting a more prominent
role of the A genome towards domestication of this crop. The east European line, Heera, carried favorable alleles for 42%
of the detected QTL and the remaining 58% were in the Indian gene pool line, Varuna. We observed clustering of major QTL in
a few linkage groups, particularly in J7 and J10 of the A genome, with QTL of different traits having agronomically antagonistic
allelic effects co-mapping to the same genetic interval. QTL analysis also identified some well-separated QTL which could
be readily transferred between the two pools. Based on the QTL analysis, we propose that improvement in yield could be achieved
more readily by heterosis breeding rather than by pure line breeding.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
15.
The objective of this study was to dissect the genetic control of days to flowering (DTF) and photoperiod sensitivity (PS) into the various components including the main-effect quantitative trait loci (QTLs), epistatic QTLs and QTL-by-environment interactions (QEs). Doubled haploid (DH) fines were produced from an F1 between two spring Brassica napus cultivars Hyola 401 and Q2. DTF of the DH lines and parents were investigated in two locations, one location with a short and the other with a long photoperiod regime over two years. PS was calculated by the delay in DTF under long day as compared to that under short day. A genetic linkage map was constructed that comprised 248 marker loci including SSR, SRAP and AFLP markers. Further QTL analysis resolved the genetic components of flowering time and PS into the main-effect QTLs, epistatic QTLs and QEs. A total of 7 main-effect QTLs and 11 digenic interactions involving 21 loci located on 13 out of the 19 linkage groups were detected for the two traits. 3 main-effect QTLs and 4 pairs of epistatic QTLs were involved in QEs conferring DTF. One QTL on linkage group (LG) 18 was revealed to simultaneously affect DTF and PS and explain for the highest percentage of the phenotypic variation. The implications of the results for B. napus breeding have been discussed. 相似文献
16.
Marta S. Lopes Matthew P. Reynolds C. Lynne McIntyre Ky L. Mathews M. R. Jalal Kamali Moussa Mossad Yousef Feltaous Izzat S. A. Tahir Ravish Chatrath Francis Ogbonnaya Michael Baum 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(4):971-984
Heat and drought adaptive quantitative trait loci (QTL) in a spring bread wheat population resulting from the Seri/Babax cross designed to minimize confounding agronomic traits have been identified previously in trials conducted in Mexico. The same population was grown across a wide range of environments where heat and drought stress are naturally experienced including environments in Mexico, West Asia, North Africa (WANA), and South Asia regions. A molecular genetic linkage map including 475 marker loci associated to 29 linkage groups was used for QTL analysis of yield, days to heading (DH) and to maturity (DM), grain number (GM2), thousand kernel weight (TKW), plant height (PH), canopy temperature at the vegetative and grain filling stages (CTvg and CTgf), and early ground cover. A QTL for yield on chromosome 4A was confirmed across several environments, in subsets of lines with uniform allelic expression of a major phenology QTL, but not independently from PH. With terminal stress, TKW QTL was linked or pleiotropic to DH and DM. The link between phenology and TKW suggested that early maturity would favor the post—anthesis grain growth periods resulting in increased grain size and yields under terminal stress. GM2 and TKW were partially associated with markers at different positions suggesting different genetic regulation and room for improvement of both traits. Prediction accuracy of yield was improved by 5 % when using marker scores of component traits (GM2 and DH) together with yield in multiple regression. This procedure may provide accumulation of more favorable alleles during selection. 相似文献
17.
Comparative mapping of QTLs for agronomic traits of rice across environments by using a doubled-haploid population 总被引:11,自引:0,他引:11
Chaofu Lu Lishuuang Shen Ping He Ying Chen Lihuang Zhu Zhenbo Tan Yunbi Xu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(1):145-150
We report here the RFLP mapping of quantitative trait loci (QTLs) which affect some important agronomic traits in cultivated
rice. An anther culture-derived doubled-haploid (DH) population was established from a cross between indica and japonica rice
varieties. A molecular linkage map comprising 137 markers was constructed based on this population which covered the rice
genome at intervals of 14.8 cM on average. The linkage map was used to locate QTLs for such important agronomic traits as
heading date, plant height, number of spikelets per panicle, number of grains per panicle, 1 000-grain weight and the percentage
of seed set, by interval mapping. Evidence of genotype-by-environment interaction was found by comparing QTL maps of the same
population grown in three diverse environments. A total of 22 QTLs for six agronomic traits was detected which were significant
in at least one environment, but only seven were significant in all three environments; seven were significant in two environments
and eight could only be detected in a single environment. However, QTLs-by-environment interaction was trait dependent. QTLs
for spikelets and grains per panicle were common across environments while traits like heading date and plant height were
more sensitive to environment.
Received: 22 February 1996 / Accepted: 10 May 1996 相似文献
18.
Stein CM Schick JH Gerry Taylor H Shriberg LD Millard C Kundtz-Kluge A Russo K Minich N Hansen A Freebairn LA Elston RC Lewis BA Iyengar SK 《American journal of human genetics》2004,74(2):283-297
Speech-sound disorder (SSD) is a complex behavioral disorder characterized by speech-sound production errors associated with deficits in articulation, phonological processes, and cognitive linguistic processes. SSD is prevalent in childhood and is comorbid with disorders of language, spelling, and reading disability, or dyslexia. Previous research suggests that developmental problems in domains associated with speech and language acquisition place a child at risk for dyslexia. Recent genetic studies have identified several candidate regions for dyslexia, including one on chromosome 3 segregating in a large Finnish pedigree. To explore common genetic influences on SSD and reading, we examined linkage for several quantitative traits to markers in the pericentrometric region of chromosome 3 in 77 families ascertained through a child with SSD. The quantitative scores measured several processes underlying speech-sound production, including phonological memory, phonological representation, articulation, receptive and expressive vocabulary, and reading decoding and comprehension skills. Model-free linkage analysis was followed by identification of sib pairs with linkage and construction of core shared haplotypes. In our multipoint analyses, measures of phonological memory demonstrated the strongest linkage (marker D3S2465, P=5.6 x 10(-5), and marker D3S3716, P=6.8 x 10(-4)). Tests for single-word decoding also demonstrated linkage (real word reading: marker D3S2465, P=.004; nonsense word reading: marker D3S1595, P=.005). The minimum shared haplotype in sib pairs with similar trait values spans 4.9 cM and is bounded by markers D3S3049 and D3S3045. Our results suggest that domains common to SSD and dyslexia are pleiotropically influenced by a putative quantitative trait locus on chromosome 3. 相似文献
19.
Comparison of single seed descent and anther culture-derived lines of three single crosses of rice 总被引:2,自引:0,他引:2
B. Courtois 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,85(5):625-631
Summary Distribution parameters (mean, variance, skewness and kurtosis) of 12 quantitative traits were evaluated for inbred lines generated through single seed descent (SSD) and anther culture (AC) of two japonica x japonica hybrids and one japonica x indica hybrid of rice. For most of the traits the data were normally distributed, and the means and variances were found to be identical for SSD- and AC-derived lines of the given hybrids. However, for some other traits, differences between the two population types were observed, mainly in the lines derived from the intra-japonica crosses. A tentative explanation for these differences is given. Nevertheless, our results suggest that SSD and AC are equally effective breeding techniques for producing agronomically useful lines of rice. 相似文献
20.
Anther culture was used to generate microspore-derived doubled haploid (DH) plants from four spring barley crosses. The culture medium used contained maltose as the sole carbohydrate source and the mode of plantlet regeneration was mainly via pollen embryogenesis. Both haploid and spontaneously doubled regenerants were produced and the doubled haploids were compared to recom-binant inbred lines generated by several rounds of selfing (single seed descent). Parental, DH and single seed descent (SSD) lines were grown in randomised, replicated field trials and the samples were scored for a range of agronomic traits. The mean performance and phenotypic distribution of the DH and SSD samples were similar and there was little evidence to support the conclusion that anther culture derived lines exhibit a reduction in vigour. Where significant differences were detected between groups these were mainly confined to crosses which were segregating for the denso dwarfing gene. The differential transmission of particular regions of the barley genome may therefore influence and confound the expression of agronomic traits in DH populations. This is the first report of the agronomic performance of anther culture lines produced via pollen embryogenesis and the results are discussed in relation to the exploitation of anther culture technology in barley breeding. 相似文献