内蒙古草原植物的营养元素特征

对内蒙古草甸草原、典型草原和荒漠草原79种主要植物的氮、钾、钙、磷、硫、铁、锌、硼、锰、铜、钼等12种营养元素的研究结果表明:1.植物体内不同营养元素含量有不同的水平。氮、钾、钙最高,其含量变化在1—10%之间;钼最低,其含量变化在0.1—3ppm 之     

紫胶生产对土壤及寄主植物营养元素含量和分布的影响

土壤、植物中的营养元素含量特征是生态系统养分循环的重要内容之一,为探讨人工紫胶林中紫胶生产对土壤及寄主植物中营养元素含量和分布的影响,本研究调查了不同强度紫胶生产样地中植物根、茎、叶、枝条各部位以及土壤不同深度的氮、磷、钾营养元素含量。结果显示:不同强度的紫胶生产会显著影响植物氮的含量,但是对磷和钾没有显著的影响。低强度紫胶生产样地的植物含氮量显著高于高强度紫胶生产和无生样地植物的含氮量。不同强度的紫胶生产对土壤中全氮、速效磷的含量无显著影响,但是对全磷、全钾和速效钾有显著影响,高强度紫胶生产样地土壤中全磷和全钾的含量都显著低于无生产样地。本研究揭示了紫胶林生态系统中低强度的紫胶生产会提高植物中氮元素的含量,同时紫胶生产会降低土壤中磷和钾的含量,对紫胶生产的持续经营有一定指导意义。     

不同退化阶段高寒草甸土壤化学计量特征

为了阐明不同退化阶段高寒草甸土壤的化学计量特征,沿着高寒草甸退化的梯度选取了原生嵩草草甸、轻度退化草甸和严重沙化草甸,测定了高寒草甸退化过程中不同深度土壤的有机碳、全氮、全磷和全钾含量。结果表明:随着高寒草甸的退化,0～100cm土壤的有机碳、全氮、全磷和全钾含量以及碳氮比、碳磷比、碳钾比、氮磷比、氮钾比和磷钾比均呈降低趋势,且土壤有机碳对高寒草甸退化的敏感性最高,全氮、全磷和全钾的敏感性依次降低,表层20cm的土壤有机碳和全氮可作为表征高寒草甸退化程度最敏感的土壤养分指标。另外,随着草甸的退化,土壤的有机碳、全氮、全磷和全钾含量及其化学计量比的垂直分布明显不同:随着土壤深度的增加,原生嵩草草甸和轻度退化草甸的土壤有机碳、全氮和全磷含量以及碳氮比、碳磷比、碳钾比、氮磷比、氮钾比和磷钾比在0～40cm范围内锐减,在40cm以下缓慢降低并趋于稳定;而沙化草甸土壤的有机碳、全氮、全磷和全钾及其化学计量比随着土壤深度的增加保持不变。     

油茶林生态系统中营养元素循环的研究

本文研究了两种优化经营措施油茶林中营养元素的含量、净积累和循环,以综合措施的油茶林作一实例,分析了生态系统中营养元素的循环过程,并提出了氮、磷、钾元素的循环局部动态模型。结果表明:(1)油茶林各器官的营养元素的含量呈现出氮>钾>镁>钙>磷的趋势,最高的是果或叶,最低的是树干。(2)当油茶林采取优化经营管理后,器官养分随之增高。如综合措施一年生叶氮、磷、钾含量分别高于对照的32.5％、26.5％、8.6％;而一年生枝中氮、磷、钾含量分别高于对照的26.5％、33.9％、1.7％。这更进一步证实了优化措施之所以产果量高,就是因为积累了充分养分,保证了生长发育的需要这一点。     

人体有多少矿物

目前已知92种天然元素中,人体内测出的已有六十余种。分为:一、常量元素:计有碳、氢、氧、氮、硫、磷、钾、钠、钙、镁、氯等11种,占人体重量的99%以上。其中氧占人体重量的62%—81%,碳占19.37%,钙占11.39%,氢占9.31%,氮占4.82%,钾、钠占0.48%,硫占0.64%,磷占0.98%。常量元素是人体(生物)所必需的营养物质之一。是构成细胞、血液、骨髂、肌肉和脏器等的主要组成成分。例如人体主要是由水份(占70—90%)和钙质(占10—20%)所组成。故又称为生命组成元     

辽东山区次生林林窗大小对土壤微生物量碳、氮、磷的影响

林窗干扰是次生林更新和演替过程的重要干扰类型,影响土壤的养分供应。为明确温带次生林林窗大小对土壤养分循环过程的影响,以辽东山区典型次生林内形成时间为6年的大、中、小(90~670 m~2)人工林窗为研究对象,测定了生长季不同大小林窗内0~20cm土层土壤微生物量碳、氮、磷。结果表明:与对照样地相比,大、中、小林窗0~10和10~20 cm土层土壤微生物量碳、微生物量磷、微生物量碳/全碳和微生物量磷/全磷无显著差异,且不同大小林窗间土壤微生物量碳、氮、磷以及微生物量碳/全碳、微生物量氮/全氮、微生物量磷/全磷均无显著差异。与对照样地相比,中林窗(290 m~2)10~20 cm土层土壤微生物量氮和微生物量氮/全氮显著升高,表明中林窗促进了10~20 cm土层土壤氮素的循环和有效氮素的供应。     

黄河三角洲盐碱地不同造林模式下的土壤碳氮分布特征

为了探讨不同造林模式对土壤碳氮影响的生态效应,以黄河三角洲盐碱地白蜡+棉花(FC)、香花槐+棉花(RC)、竹柳+棉花(SC)、白蜡林(F)、竹柳林(S)等5种造林模式为研究对象,分析比较各造林模式土壤的碳氮形态及分布特征,为重度退化刺槐林的经营改造和造林模式选择提供理论依据。结果显示:(1)不同造林模式下土壤的可溶性总碳和可溶性有机碳含量均高于裸地,农林间作高于纯林,其中SC模式含量最高,其次为FC和RC模式,而F和S纯林模式较低;5种造林模式不同土层可溶性有机碳含量均表现为0—20 cm高于20—40 cm。(2)农林间作模式0—40 cm土层的可溶性全氮和可溶性有机氮平均含量均高于纯林模式,其中SC模式的含量最高。FC、SC和S纯林模式0—20 cm土层可溶性有机氮含量显著高于20—40 cm土层,分别为其1.4、1.5和2.7倍;而RC模式20—40 cm土层可溶性有机氮含量显著高于0—20 cm土层。(3)5种造林模式中,除F纯林土壤硝态氮低于裸地外,其他造林模式下的土壤各种氮养分含量均显著高于裸地。土壤可溶性有机碳与全氮和铵态氮的相关性达到极显著水平(P0.01)。研究表明农林间作模式可显著提高重度退化刺槐林皆伐后土壤中有效态碳、氮含量,其中SC模式改良效果较好,而纯林模式较差。     

中国南亚热带不同造林模式碳汇林土壤碳、氮、磷的积累及化学计量特征

碳汇林的固碳效益十分显著,是实现“碳中和”的主要碳增汇途径之一,在研究南亚热带地区不同造林模式碳汇林土壤碳汇的同时,研究碳汇林土壤氮、磷储量及土壤碳、氮、磷的生态化学计量特征,能够为不同造林模式碳汇林的土壤碳、氮、磷储量的评估提供科学依据。采取单因素随机区组设计,选择立地条件基本一致的研究区和4种造林模式(新造林、封山育林、补植套种、更新改造)的碳汇林,分析了不同造林模式土壤有机碳(SOC)、全氮(TN)、全磷(TP)的含量、储量、生态化学计量比,以及微生物生物量碳(MBC)的变化特征。结果表明,不同造林模式碳汇林的土壤理化性质,碳氮磷储量、化学计量比及微生物生物量碳含量差异显著,且土壤碳氮磷储量随土壤深度增加而减少,表现出明显的垂直变化特征。4种造林模式下碳氮比(C/N)介于11.11—17.86,碳磷比(C/P)介于17.00—242.59,氮磷比(N/P)介于1.18—15.99之间,在0—60 cm土层上均以更新改造模式林最低,封山育林模式下C/P和N/P均显著高于其他造林模式。土壤SOC与TN、N/P呈极显著正相关关系,土壤TN与C/P呈极显著正相关关系。综合来看,封山育林模...     

泥石流频发区山地不同海拔土壤化学计量特征——以云南省小江流域为例

为了探究泥石流频发区不同海拔梯度土壤的化学计量特征,阐明土壤性质对海拔变化的响应规律,进而有效指导受限性生态脆弱区生态系统的保护和恢复,在云南省小江流域支流阿旺小河西北侧山地选取了1500—2000m、2000—2500m、2500—3000m 3个海拔梯度,测定各海拔梯度范围内0—10cm、10—20cm、20—30cm土壤的有机碳、全氮、全磷、全钾含量及其机械组成,分析了不同海拔梯度土壤化学计量比的垂直分布特征及其与植被区、土壤物理结构的关系。结果表明:随着海拔梯度的升高,土壤有机碳和全氮含量以及碳磷比、碳钾比、氮磷比和氮钾比均呈升高趋势,全磷和全钾含量以及磷钾比呈降低趋势,且有机碳、全氮、全磷和全钾对海拔的敏感程度依次降低;不同海拔梯度之间,土壤有机碳、全氮、全磷、全钾及其化学计量比的垂直分布存在显著差异性,即随着土壤深度的增加,有机碳和全氮含量呈降低趋势,而全磷和全钾含量以及各化学计量比变化规律不明显;同一海拔梯度内,森林植被区和灌草丛群落植被区土壤生态化学计量比差异性不大,且随海拔梯度变化一致,灌草丛群落中,土壤碳氮比与地上植被盖度具有极显著正相关性,森林群落植被区,土壤磷钾比与优势乔木种平均高度具有极显著负相关性,而与平均胸径呈显著负相关性;土壤物理结构的分异是造成土壤化学计量特征发生变化的主要内在原因,土壤碳磷比、碳钾比、氮磷比、氮钾比随着含水率和砂粒的增加呈指数型上升而随着粘粒的增加呈指数型下降的趋势。     

长期施锌条件下土壤-植物系统植物营养元素的分布特征

对黄土高原旱地连续17a施用锌肥条件下植物营养元素在土壤-植物系统中的分布特征进行研究,结果表明：长期施用锌肥0～40cm土层土壤的碳、氮元素含量高于对照处理,而40～100cm土层土壤的碳、氮含量低于对照处理;0～100cm土层土壤全磷含量高于对照,而有效磷含量低于对照。施锌冬小麦茎、叶和籽粒中氮含量高于不施锌处理,而壳中氮含量低于对照处理,施锌后氮素在冬小麦茎、叶、籽粒和整个植株中的累积量高于对照,籽粒和茎中磷的含量低于对照,植株中磷的积累量也低于对照。     

Further studies of the sieve-element plastids of theCaryophyllales includingBarbeuia,Corrigiola, Lyallia,Microtea, Sarcobatus,andTelephium

The sieve-element plastids of 69 species of theCaryophyllales were investigated by transmission electron microscopy. All contained the specific subtype-P3 plastids characterized by a peripheral ring of protein filaments. The presence or absence of an additional central protein crystal and their shape being either polygonal or globular as well as the average sizes of the sieve-element plastids are useful features in the characterization of some families.—Barbeuia contains sieve-element plastids that confirm its placement within thePhytolaccaceae. Lyallia differs fromHectorella by including small starch grains in their sieve-element plastids, which otherwise by their globular crystals negate a closer connection to theCaryophyllaceae. The lack of a central protein crystal in its form-P3fs plastids placesMicrotea best within theChenopodiaceae. Sarcobatus, a so far uncontested member of theChenopodiaceae, contains form-P3cf plastids, i.e., including a central crystal not found elsewhere in this family.Telephium andCorrigiola, shifted back and forth betweenMolluginaceae andCaryophyllaceae, have form-P3cf(s) plastids with a polygonal crystal which favor their placement within theCaryophyllaceae.     

Synopsis of the tribe Bocageeae (Annonaceae), with revisions of Cardiopetalum,Froesiodendron, Trigynaea,Bocagea, and Hornschuchia

The tribal name Bocageeae Endlicher is reestablished and the tribe is circumscribed on the basis of solitary internodal ebracteate pedicels that are articulated at the base, and pollen shed in polyads of eight or more grains. Septate anther locules, large pollen size, and seed appendages are prevalent in the tribe. Intectate pollen with free-standing columellae, rare in Annonaceae, occurs not only in the genusTrigynaea but also in some species ofBocagea andHornschuchia. As defined here, the Bocageeae include seven neotropical genera:Cymbopetalum, Porcelia, Bocagea, Cardiopetalum, Froesiodendron, Hornschuchia, andTrigynaea. The latter five genera are revised and the treatments include ten new species:Cardiopetalum plicatum, Froesiodendron urceocalyx, Hornschuchia lianarum, H. santosii, H. leptandra, Trigynaea cinnamomea, T. lanceipetala, T. triplinervis, T. lagaropoda, andT. axilliflora, all from tropical South America. A new combination,Froesiodendron longicuspe, changes the rank of that taxon from subspecies to species.Cardiopetalum surinamense is removed fromFroesiodendron and reassigned toCardiopetalum on the basis of its connate petals, dehiscent monocarps, and seeds with bilobed arils. A cladogram provides an explicit hypothesis of intergeneric relationships in the tribe. The new combinationOnychopetalum periquino, based onTrigynaea periquino, is made.     

A reconsideration of the genusRabdosiella (Lamiaceae,Nepetoideae, Ocimeae)

The two generaPlectranthus andIsodon are compared and found to be very dissimilar.Isodon ist considered to be misplaced inOcimeae subtribePlectranthinae and apparently is more closely related to subtribeHyptidinae. The disjunct genusRabdosiella is compared to these two genera and regarded to be polyphyletic. The AfricanR. calycina (Benth.)Codd is returned toPlectranthus and calledP. calycinus Benth., while the AsianR. ternifolia (D. Don)Codd is placed inIsodon sect.Pyramidium and calledI. ternifolius (D. Don)Kudo.     

Entwicklungsgeschichte und Ultrastruktur von Pollenkitt und Exine bei nahe verwandten entomophilen und anemophilen Angiospermensippen derAlismataceae,Liliaceae, Juncaceae,Cyperaceae, Poaceae undAraceae

Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout.

     

Identification of b,c, and d cytochromes in the membrane of Vitreoscilla

Cytochromes b, c, d, and o were identified by spectroscopic analysis of respiratory membrane fragments from Vitreoscilla sp., strain C1. Carbon monoxide difference spectra of the reduced membranes had absorption maxima at 416, 534, and 571 nm (ascribed to cytochrome o) and 632 nm (cytochrome d). Derivative spectra of the pyridine hemochromogen spectra of the membranes identified the presence of b- and c-type cytochromes in Vitreoscilla. The cyanide binding curve of the membranes was biphasic with dissociation constants of 2.14 mM and 10.7 mM which were assigned to cytochrome o and cytochrome d, respectively. Membranes bound carbon monoxide with dissociation constant 3.9 M, which was assigned to cytochrome o. Cytochrome c ₅₅₆ and a NADH-p-iodonitrotetrazolium violet reductase component were partially purified from Vitreoscilla membranes.Abbreviations INT p-iodonitrotetrazolium violet - RMF respiratory membrane fragments - K _d dissociation constant - CHAPS 3-[(3-cholamido propyl) dimethylammonio]-1-propanesulfonate - DOC sodium deoxycholate - PAGE polyacrylamide gel electrophoresis - SDS sodium dodecyl sulfate     

Antibiotic Susceptibility as a Taxonomic Characteristic of Proteobacteria of the Genera Alteromonas, Pseudoalteromonas, Marinomonas, and Marinobacter

Type strains of bacteria of the genera Alteromonas, Pseudoalteromonas, Marinomonas, and Marinobacterand symbiotrophic bacteria isolated from the Far Eastern bivalve mollusks Crenomytilus grayanusand Mizuhopecten yessoensisand identified as Pseudoalteromonas nigrifaciensand P. citreawere tested for susceptibility to antibiotics. Of the ten antibiotics used in this study, benzylpenicillin, polymyxin, lincomycin, and oleandomycin were valuable for the polyphasic taxonomy of marine aerobic proteobacteria. Marine bacteria of the genera studied exhibited characteristic antibiotic susceptibility profiles, which were useful for the differentiation of the genera.     

Association of polymorphisms of cytokine genes (IL1B, IL1RN, TNFA, LTA, IL6, IL8, and IL10) with chronic obstructive pulmonary disease

To assess the role that polymorphisms of cytokine genes play in genetic predisposition to chronic obstructive pulmonary disease (COPD), the allele and genotype distributions of IL1B, IL1RN, TNFA, LTA, IL6, IL8, and IL10 were studied in COPD patients (N = 319) and healthy individuals (N = 403), residents of Ufa, Bashkortostan. Genotype IL1RN*2/IL1RN*2 of IL1RN was identified as a risk factor for COPD, its frequency being 9.80% in the COPD patients and 4.67% in the healthy subjects (x ² = 5.45, df = 1, P = 0.02, OR = 2.21). Genotype GG of the LTA polymorphism A252G was significantly more common in the COPD patients than in the controls (7.84% vs. 3.72%; x ² = 5.00, df = 1, P = 0.026). In patients with COPD stage IV, the frequency of this genotype was twice as high as in those with COPD stages II and III (11.18% vs. 4.79%; x ² = 3.08, df = 1, P = 0.08). Genotype GG of the TNFA polymorphism G(?308)A in combination with genotype AA of the LTA polymorphism A252G was significantly less frequent in the COPD patients than in the healthy subjects (38.55% vs. 46.93%; x ² = 8.82, df = 1, P = 0.0039). Genotype GG of the IL6 polymorphism G(?174)C was more frequent in the patients with COPD stage IV (43.75% vs. 31.54% in the patients with COPD stages II and III, x ² = 4.15, P = 0.042). No significant differences were found between the groups of COPD patients and healthy subjects concerning the genotype frequencies of the polymorphisms T(?511)C and T3953C of IL1B, G(?308)A of TNFA, G(?174)C of IL6, A(?251)C of IL8, and C(?627)A of IL10.     

Lack of relationship between gliding cyanobacteria and filamentous gliding heterotrophic eubacteria: comparison of 16S rRNA catalogues of Spirulina,Saprospira, Vitreoscilla,Leucothrix, and Herpetosiphon

Spirulina platensis and Spirulina maxima were characterized by 16S ribosomal RNA oligonucleotide cataloguing. Both species are highly related. They fall into the main group of the cyanobacteria, showing a remote relationship to Nostoc, Fischerella, Aphanocapsa, and also to Prochloron. Low similarity coefficients were found between the Spirulina species and certain organotrophic and sulfide oxidizing bacteria, viz. Saprospira grandis, Vitreoscilla stercoraria, Leucothrix mucor, Herpetosiphon aurantiacus, and Beggiatoa leptomitiformis, respectively. This result does not support the classical hypothesis that certain filamentous gliding bacteria are apochlorotic cyanobacteria.     

Isolation,Biological Properties,and Spatial Structure of Antibiotic Loloatin A

A peptide antibiotic with cyanolytic activity was isolated from the IGM52 strain of the Brevibacillus laterosporus Gram-positive spore-forming bacteria. By ¹H NMR spectroscopy, this antibiotic was identified as loloatin A, a cyclic decapeptide cyclo(-Asn-Asp-Tyr-Val-Orn-Leu-D-Tyr-Pro-Phe-D-Phe-). The spatial structure of loloatin A in solution was determined.     

Enhancement of sporulation in species of Bipolaris, Curvularia, Drechslera, and Exserohilum by growth on cellulose-containing substrates

Nine species of Bipolaris, Curvularia, Drechslera, and Exserohilum were compared for sporulation on agar media and for enhancement of sporulation by growth on four cellulose-containing substrates (index card, filter paper, cheesecloth, cotton fabric). On two natural and one synthetic agar media, sporulation varied from profuse to nonexistent among three isolates of each species. Growth of all species on cellulose substrates resulted in large and significant increases in sporulation. Growth on index card pieces often provided the greatest increases, but no single substrate was superior for all species, and significant substrate × isolate interactions were observed within species. Overlay of filter paper onto whole colonies in agar plates resulted in 2 to 18-fold increases in sporulation for eight of nine species and production of spores in sufficient quantity for most experimental purposes. Overlay of soil dilution plates with filter paper to promote sporulation of colonies enabled detection of B. spicifera, B. hawaiiensis, C. lunata, and E. rostratum at relatively low population levels (≤1.3 × 10³ colony-forming units per gram of soil) in samples of a naturally infested soil. Results indicate that enhancement of sporulation by growth of species of Bipolaris, Curvularia, Drechslera, and Exserohilum on cellulose substrates may facilitate (i) their identification in culture, (ii) production of spores at relatively high concentrations, and (iii) detection and enumeration of these fungi in soil.