福建汉族8个红细胞血型系统的分布

对福建汉族人群红细胞血型系统的19个抗原进行了调查。各系统的调查人数与基因频率为:ABO:216人,p=0.1936、q=0.1766,r=0.6298;Lewis:214人,Le(a+)17人,表现型频率=7.94％;P:215人,n=0.1427;Diego:215人,Di(?)=0.2830;MNSs:用抗M、抗N血清调查了324人,对其中150人又用S和s抗血清进行了调查,m=0.5695、n=0.4305、单倍型频率MS=0.0200、NS=0.0139,Ms=0.5500、Ns=0.4161;Dhffy:214人,发现Fy(a-)3例;Fy(?)=0.8817;Kidd:215人,未发现JK(a-b=)型,JK(?)=0.4767;Rh:214人,发现1例CCdee型,d=0.0686,单倍型频率r(?)=0.0686、R~1=0.6352、R~2=0.1970、R~0=0.0605、R~2=0.0388。     

A survey of phenotype distribution of subgroup A2, blood groups MNS, P, and Kell in the Kazak nationality living in northern Xinjiang of China

The research was undertaken to study the phenotypic polymorphisms of the subgroup A2, blood groups MNS, P, and Kell in the Kazakh population in northern Xinjiang, China and establish data on rare blood group antigens in the Kazakh population, in order to provide references for clinical blood transfusion safety and prevention of hemolytic disease of the new born. In this study, 6,862 unrelated Kazakh individuals in northern Xinjiang were randomly selected, and their blood samples were collected for serological testing. The antigens of A, B, A1, M, N, P1 and K were detected by serological saline tube method, and the antigens of S, s, and k were detected by the microcolumn gel antiglobulin card method. The results were as follows: ① The detection rates of subgroup A2 in group A and group AB were 7.08% and 21.79%, respectively; ② The allele frequencies of the blood groups MNS, P and Kell were M=0.5668, N=0.4332, S=0.1860, s= 0.8140, P1=0.2848, P2=0.7152, K1=0.0096, K2=0.9904. The observed values and expected values of frequency distribution of genotypes were compared by χ² test, which conformed to the Hardy-Weinberg genetic law (P>0.05); ③ Fourteen cases of S^-s^- rare phenotype were detected in MNS blood group system, with a frequency of 1.16%; ④ The frequency of K antigen in the Kell blood group system was 1.92%. One case of rare KK homozygote was detected, with a frequency of 0.034%. Our study suggested that the distribution of gene frequency of subgroup A2, blood groups MNS, P and Kell in the Kazakh population in northern Xinjiang has its own characteristics, and their blood group MNS has unique genotypes. The positive rate of K antigen of blood group Kell in the Kazakh population was significantly higher than Chinese Han population.     

Estimation of stature by using the dimensions of the right hand and right foot in Han Chinese adults

The Han Chinese people are the main ethnic group in China and the largest ethnic group in the world.The dimensions of the hands and feet have been successfully used for the estimation of stature.A total of 26,927 healthy adult subjects,comprising 13,221 men and 13,706 women,were recruited.The survey samples were chosen through random cluster sampling.The mean values were significantly higher in men than those in women for all measurements(P0.001).All the measurements showed a statistically significant correlation with stature(P0.01).The combination of the right hand length and the right foot length was the best predictor of stature because it had the lowest standard error of estimate.The use of multiple regression equations yielded better results than did the use of linear regression equations.The accuracy of stature prediction ranged from ±4.81 to ±6.39 cm.The present study was of great importance with regards to improving the physical anthropology database of ethnic groups in China.     

贵州汉族八个红细胞血型系统的分布

本文调查了贵州汉族八个红细胞血型系统的分布。结果表明P_1基因频率(0.1520)和Di~a。基因频率(0.0182)较低。其它系统的基因频率为P=0.2171、q=0.1858、r=0.5969、m=0.5400、n=0.4600、S=0.0478、s=0.9522、Jk~a=0.4633、Jk~b=0.5367、Fy~a=0.9367、Fy~b=0.0633。MNSs血型系统单倍型频率为MS=0.0301、NS=0.0177、Ms=0.5099、Ns=0.4423。Rh血型系统单倍型频率为R~1=0.5766、R~2=0.2508、R~0=0.0511、R~z=0.0421、r~’=0.0794。未发现MMSS、MNSS及Fy(a-)表现型。     

贵州三都水族Y染色体单倍型频率分析

在92例贵州三都水族个体中,用PCR-RFLP法研究由11个单核苷酸多态位点(SNPs)组成的Y染色体单倍型频率分布,结果显示该人群的Y染色体主要为南方特异的H11和H9单倍型,两者频率高达90.22％。主成分分析结果显示其父系遗传结构与我国黎族、布依族等汉藏语系壮侗语族民族最为接近。通过Y染色体的遗传学观察与历史记载和语言学分类有较好一致性。 Abstract:Non-recombination region of Y-chromosome is a useful marker in tracing evolutionary history of paternal lineage.In the present study,total 92 individuals from Shui ethnic group in Sandu Shui Ethnic Group Autonomous County of Guizhou Province were inspected with 11 SNP sites including M7,M9,M15,M45,M89,M95,M119,M122,M130,M134 and YAP on Y-chromosome.All the subjects were required to be unrelated and without intermarriage with other ethnic groups within three generations.The haplotypes were analyzed by PCR-RFLP method.Four haplotypes H5,H8,H9 and H11 were detected with frequencies of 0.054,0.044,0.315 and 0.587,respectively.Principle component indicated that the paternal lineage of Shui ethnic group is much closer to Li ethnic group of Hainan Province and Bouyei ethnic group of Guizhou Province,which belong to the group of Zhuang-Dong branch of Sino-Tibetan language family.In addition genetic study of Shui coincides with its linguistic distribution.     

中国畲族群体D17S30位点遗传多态性研究

采用聚合酶链反应 (PCR)技术对中国畲族群体270名无关个体D17S30位点的VNTR进行了研究,共检出12个等位基因,片段大小为168bp-1008bp,基因频率为0.0056-0.3259,杂合度为79.10%,父权排除率为0.4358。家系分析表明,扩增片段按孟德尔方式遗传。统计学分析证明,本资料符合Hardy-W einberg平衡定律(x2=79.97,v=66,P=0.1158>0.05)。 Abstract:The variable number of tandem repeat(VNTR)of D17S30 locus in 270 unrelated individuals of She ethnic group was studied by polymerase chain reaction(PCR).The results showed that 12 alleles were detected and their sizes ranged from 168bp to 1 008bp.The allele frequencies were 0.0056~0.3259,the heterozygosity of this locus was 79.10% and the paternity exclusion probability was 0.4358.The number of D17S30 VNTR allele and allele frequency of She were different from those of Han ethnic group in Mendelian Law.Statistical analysis demonstrated that their geneotypes were consistent with Hardy-Weinberg equilibrium(x2=79.97,v=66,P=0.1158>0.05).     

中国17个人群中的耵聍基因频率及干型基因地理分布图

报道了中国17个人群的干型耵聍基因频率,其中汉族人群8个,少数民族人群8个,还有一个未识别民族,即西藏聂拉木县的夏尔巴人。发表了我国汉族人群中以及汉族与少数民族中干型耵聍基因频率的地理分布图。干型耵聍基因在17个人群中的频率及两张基因频率地理分布图都进一步证明,中国汉族与少数民族 (新疆信仰伊斯兰教的少数民族除外)之间有许多基因流动、干型耵聍基因起源于东北亚。中国人群中干型耵聍基因的Fst应在0.1057与0.1602之间。 Abstract:Gene frequencies of serumen type of 17 populations in China are reported,including 8 Han subpopulations,8 ethnic minorities and 1 un-identified ethnic group,the Sherpas in Nyalam County,Tibet.Gene-frequency-distribution maps of dry serumen in Han,as well as in Han and ethnic minorities of China were also published.The gene frequency data of 17 populations and their distribution maps once again showed that there was a large-scale gene exchange between Han and ethnic minorities,my be,with the exception of Moslem ethnic groups in Xinjiang,and that the dry serumen gene originated in Northeast Asia.The Fst of dry serumen gene in Chinese populations as a whole was estimated to be between 0.1057 and 0.1602.     

Relationship of HLA-A, -Cw Polymorphisms with HIV/AIDS in Chinese Yi Ethnic Group of Sichuan Province1

The relationship of HLA-A, -Cw alleles on HIV infection and AIDS disease progression in the Chinese Yi ethnic group of Sichuan province were investigated. The genetic polymorphisms of HLA-A, -Cw alleles of 102 unrelated healthy Chinese Yi ethnic individuals, 68 HIV-1 infected and 21 HIV positive long-time survivors were typed by PCR-SSP assay. Statistic signifiance was determined by the χ^2 test with the SPSS software. No significant differences were observed between the HLA-A, -Cw alleles of the 68 HIV-1 infected and 102 non-infected Chinese Yi control individuals. Whereas the prevalence of A＊3601,Cw＊14（01-03）and Cw＊0304 was significantly higher in 21 long time survivors compared with 102 healthy controls with P values of 0.016, 0.016 and 0.000 by χ^2 or the Fisher exact test respectively. The result implies that A＊3601,Cw＊14（01-03） and Cw＊0304 may be associated with slow AIDS disease progression in the Chinese Yi ethnic group, further studies on this association may yield insight on the pathogenesis of HIV-1 infection.     

Molecular genetic analysis of weak B blood group allele in Jinan population reveals distribution of ABw phenotype

The aim of this study was to investigate the distribution of the ABw phenotype of ABO blood group in the Jinan population. 31 856 samples were tested during the period 2018 to 2019. Thirty-nine samples with discrepant results, as identified by micro-column gel method, were further investigated by serological (tube technique) and molecular (fluorescence PCR, DNA sequencing) methods. Eight samples showed ABw phenotype, which accounted for 0.025% of the population tested. From the sequencing analysis, six samples (6/8) were typed as ABO*A1.02/ABO*BW.12 and two samples (2/8) as ABO*A1.02/ABO*BW.03. The study suggests that ABw12 account for 75% of ABw phenotype and indicate ABw12 is the main ABw phenotype in Jinan population.     

Salivary immunoglobulin classes in Nigerian smokers with periodontitis

AIM:To determine the levels of salivary immunoglobulin classes in Nigerian smokers and non-smokers with periodontitis.METHODS:Sixty-nine individuals were recruited into this study after obtaining informed consent.They were subdivided into three groups that consisted of 20(aged 46 ± 11 years) cigarette smokers with periodontitis(S+P);24(40 ± 12 years) smokers without periodontitis(S-P);and 25(53 ± 11 years) non-smokers with periodontitis(NS+P).An oral and maxillofacial surgeon used radiographs for periodontal probing for the diagnosis of periodontitis.The smokers included subjects who smoked at least six cigarettes per day and all the periodontitis patients were newly diagnosed.About 5 mL of unstimulated saliva was expectorated by each subject into plain sample bottles.Salivary immunoglobulin levels were estimated using enzyme linked immunosorbent assay.Student's t test was used to deter-mine significant differences between the means.Values of P 0.05 were regarded as significant.RESULTS:No significant differences were observed in the mean salivary levels of the immunoglobulin classes(IgG,IgA,IgM and IgE) when S+P was compared with S-P.Mean salivary levels of IgA(520.0 ± 155.1 ng/mL vs 670.0 ± 110 ng/mL,P = 0.000) and IgM(644.5 ± 160.0 ng/mL vs 791.4 ± 43.7 ng/mL,P = 0.000) were significantly lower in the S+P compared with NS+P group.Salivary IgA(570.4 ± 145.6 ng/mL vs 670.0 ± 110 ng/mL,P = 0.008) and IgM(703.1 ± 169.3 ng/mL vs 791.4 ± 43.7 ng/mL,P = 0.012) levels were significantly lower in the S-P compared with NS+P group.Only one(5%) periodontal patient had detectable levels of salivary IgE(0.20 IU/mL).Similarly,only one smoker(4.17%) had detectable levels of salivary IgE(0.04 IU/mL) and two non-smokers(9.52%) had detectable levels of IgE(0.24 IU/mL).CONCLUSION:Our study suggests that reduced salivary IgA and IgM levels in smokers with periodontitis could enhance increased susceptibility to periodontitis.     

Characterization of glucose-6-phosphate dehydrogenase deficiency and identification of a novel haplotype 487G>A/IVS5-612(G>C) in the Achang population of southwestern China

The prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency and its gene mutations were studied in the Achang population from Lianghe County in Southwestern China. We found that 7.31% (19 of 260) males and 4.35% (10 of 230) females had G6PD deficiency. The molecular analysis of G6PD gene exons 2―13 was performed by a PCR-DHPLC-Sequencing or PCR-Sequencing. Sixteen inde-pendent subjects with G6PD Mahidol (487G>A) and the new polymorphism IVS5-612 (G>C), which combined into a novel haplotype, were identified accounting for 84.2% (16/19). And 100% Achang G6PD Mahidol were linked to the IVS5-612 C. The percentage of G6PD Mahidol in the Achang group is close to that in the Myanmar population (91.3% 73/80), which implies that there are some gene flows between Achang and Myanmar populations. Interestingly, G6PD Canton (1376G>T) and G6PD Kaiping (1388G>A), which were the most common G6PD variants from other ethnic groups in China, were not found in this Achang group, suggesting that there are different G6PD mutation profiles in the Achang group and other ethnic groups in China. Our findings appear to be the first documented report on the G6PD genetics of the AChang people, which will provide important clues to the Achang ethnic group origin and will help prevention and treatment of malaria in this area.     

Genetic polymorphism of mitochondrial DNA HVS-I and HVS-Ⅱ of Chinese Tu ethnic minority group

We analyzed the two hypervariable segments HVS-Ⅰ and HVS-Ⅱ of 108 Chinese Tu ethnic minority group samples for forensic and population genetics purposes.Comparing with Anderson sequence,79 polymorphic loci in HVS-Ⅰ and 40 in HVS-Ⅱ were found in Chi-nese Tu ethnic minority group mtDNA sequences,and 90 and 64 haplotypes were then defined.Haplotype diversity and the mean pair-wise differences were 0.9903:±0.0013 and 5.7785 in HVS-Ⅰ,and 0.9777±0.0013 and 3.5819 in HVS-Ⅱ,respectively.By analyzing the hypervariable domain from nucleotide 1,6180 to 1,6193 in HVS-Ⅰ,we defined some new types of sequence variations.We also compared the relationship between Tu population and other populations using mtDNA HVS-Ⅰ sequences.According to Rst genetic distances,the phylogenetic tree showed that the Tu population,the Xi'an Han population,the Chinese Korean,and the Mongol ethnic group were in a clade.This indicated a close genetic relationship between them.There were far relations between the Tu population and other Chinese southern Han populations,Siberian,European,African,and other foreign populations.The results suggest that Tu population has a multi-origin and has also merged with other local populations.     

Genetic polymorphism of mitochondrial DNA HVS-I and HVS-Ⅱ of Chinese Tu ethnic minority group

We analyzed the two hypervariable segments HVS-Ⅰ and HVS-Ⅱ of 108 Chinese Tu ethnic minority group samples for forensic and population genetics purposes.Comparing with Anderson sequence,79 polymorphic loci in HVS-Ⅰ and 40 in HVS-Ⅱ were found in Chi-nese Tu ethnic minority group mtDNA sequences,and 90 and 64 haplotypes were then defined.Haplotype diversity and the mean pair-wise differences were 0.9903±0.0013 and 5.7785 in HVS-Ⅰ,and 0.9777±0.0013 and 3.5819 in HVS-Ⅱ,respectively.By analyzing the hypervariable domain from nucleotide 1,6180 to 1,6193 in HVS-Ⅰ,we defined some new types of sequence variations.We also compared the relationship between Tu population and other populations using mtDNA HVS-Ⅰ sequences.According to Rst genetic distances,the phylogenetic tree showed that the Tu population,the Xi'an Han population,the Chinese Korean,and the Mongol ethnic group were in a clade.This indicated a close genetic relationship between them.There were far relations between the Tu population and other Chinese southern Han populations,Siberian,European,African,and other foreign populations.The results suggest that Tu population has a multi-origin and has also merged with other local populations.     

Association between serum somatostatin levels and glucose-lipid metabolism in the Jino ethnic minority and Han Chinese population

We aim to investigate the relationship between serum somatostatin(SST) levels and glucose-lipid metabolism at various stages of glucose tolerance in the Jino ethnic minority(n=111) and Han population(n=113) of Yunnan Province, southwest China.Anthropometric parameters and biochemical traits were measured. Serum SST and plasma glucagon levels were tested. Participants were divided into three subgroups: isolated fasting hyperglycemia(IFH), isolated post challenge hyperglycemia(IPH)and normal glucose tolerance(NGT). SST levels were found lower while glucagon levels were significantly higher in the Jino ethnic with IPH(P=0.0026 and P=0.0069, respectively). Fasting glucose and high density lipoprotein-cholesterol(HDL-C)levels were higher(P=0.0055 and P=0.0021, respectively) and fasting insulin levels and homeostasis model assessments β-cell function were lower(P=0.0479 and P=0.0007, respectively) in the Jino population. After adjusting for confounding factors, the serum SST level was associated with glucagon(P0.0001) in both populations. The SST level was correlated with fasting Cpeptide(P=0.0267) in Jino and HDL-C levels in Han(P=0.0079). Our findings suggest that serum SST levels and plasma glucagon levels may vary in subjects with IPH between two ethnics.     

Evolution of gp85 gene of subgroup J avian leukosis virus under the selective pressure of antibodies

Subgroup J Avian leucosis virus (ALV-J) strain NX0101 was inoculated into chicken embryo fibroblasts (CEF) monolayers in 6-well plates. The six wells of CEF inoculated with NX0101 were divided into groups A (without anti-ALV-J serum in the medium) and B (with anti-ALV-J serum in the medium), then viruses from each well of both groups were separately passed in CEF every 6 d and formed their independent passage lineages. For each lineage of both groups, gp85 genes of the viruses in the 10th, 20th and 30th passages were amplified, cloned and sequenced. The sequence data indicated that the homologies of gp85 at aa level between the primary virus and the passed viruses of different passages of 3 lineages in group A were 97.7%-99.7%; and the homologies of gp85 between the primary virus and the passed viruses of different passages of 3 lineages in group B were 93.8%-96.1%. Analysis of the ratios of nonsynonium (NS) vs synonium (S) mutations of nucleic acids demonstrated that NS/S in 3 highly variable (hr-) regions at aa#110-120, aa#141-151 and aa#189-194 of gp85 in 3 lineages of group A were 2 (8/4), 1(3/3) and 1.3 (4/3), however, NS/S in the same 3 hr-regions of group B were 4.1 (13/3), 4.7 (14/3) and 3.3 (11/3). This study is the first demonstration of influence of immune selective pressure on evolution of ALV-J gp85 by specific antibodies under the controlled in vitro experiments.     

中国东乡族9个STR基因座遗传多态性研究

选择9个STR基因座,采用四色荧光标记STR基因扫描技术,对中国甘肃省特有民族——东乡族的群体遗传多态性进行研究。同时检测94个无关个体血液样本,共检出72种等位基因,基因频率的分布在0.0053~0.5825之间;检出182种基因型,基因型频率分布在0.0106~0.2660之间;9个STR位点基因型分布均符合Hardy-Weinberg平衡定律（P＞0.05）。9个STR位点多态信息量（polymorphism information content,PIC）均大于0.6378,杂合度(heterozygosity,H) 均大于0.6500,个体识别力(discrimination power,DP)均大于0.8216,非父排除率(probabilities of paternity exclusion,PPE) 均大于 0.4903。种族比较结果显示,甘肃东乡族与白种人及黑种人在绝大多数位点存在显著差异（P＜0.05）,而9个STR位点与汉族群体的遗传差异均不显著（P＞0.05）。研究结果丰富了中华民族基因数据库,在人类群体遗传学及法医学研究领域有重要应用价值。 Abstract:Genetic distribution for nine STR loci was determined in a Chinese Dongxing ethnic group based on STR genescan marked by fluorescence.Seventy-Two alleles and 182 genotypes were observed in 94 unrelated Chinese Dongxiang individuals,with the corresponding gene frequency and genotype frequency being 0.0053~0.5825 and 0.0106~0.2660 respectively.The genotypes of nine STR loci were in accordance with the Hardy-Weinberg equilibrium (P＞0.05).The statistical analysis of nine STR loci showed PIC( polymorphism information content,PIC)≥0.6378,H(heterozygosity,H) ≥0.6500,DP (discrimination power,DP) ≥0.8216,PPE(probabilities of paternity exculation,PPE) ≥0.4903.The result indicated that there was a significant difference between Dongxiang ethnic group and the white and the black.There was no significant difference in Han nationality.These result filled the Dongxiang ethnic group-a specific group of Chinese into the genetic database and played an important role in Chinese population genetic study and forensic medicine application.     

云南澜沧拉祜族HLA-DRB1基因多态性研究

采用我们改进的高分辨率基于内含子的PCR-SBT分型方法,首次检测云南拉祜族HLA-DRB1基因多态性。在55例拉祜族个体中共检出16种HLA-DRB1等位基因,最常见的DRB1等位基因是HLA-DRB1*12021、09012、15011,基因频率分别为30.909%、15.455%、13.636%,共占拉祜族可检出等位基因的60%,其中DRB1*0413、11081、1312、1418、1504首次在我国人群中检出,并且在世界各地人群中也比较罕见。对拉祜族和世界各地人群的HLA-DRB1频率进行了比较,分析了HLA-DRB1等位基因在各人种中的分布特点,并用Neighbor-joining法进行了聚类分析。比较分析的结果显示拉祜族明显属于中国南方族群,未显示出其族源来自北方的痕迹。对此遗传数据和民族学、历史学研究的矛盾,做了初步的分析。 Abstract:The HLA-DRB1 gene polymorphism in Lahu ethnic of Yunnan,China was the first time investigated using high resolution PCR-SBT method,which is based on sequences of HLA-DRB1 Intron 1 and Intron 2 and with our improvement.From 55 individuals of Lahu ethnic 16 DRB1 alleles were detected.The three most common alleles were HLA-DRB1*12021(30.909%),09012(15.455%),15011(13.636%),and they covered 60% of the total alleles detected from Lahu ethnic.HLA-DRB1*1413,*11081,*1312,*1418,*1504 were the first time detected in the Chinese,and were very rare in worldwide ethnic groups.With comparison of HLA-DRB1 gene frequencies between various ethnic groups we analysized the characteristics of HLA-DRB1 gene distribution in worldwide populations,and constructed the phylogenetic tree by Neighbor-joining method and Nei measure of genetic distance.The result showed Lahu ethnic obviously belong to the Chinese South ethnic groups and can't trace its origin from northern groups with the HLA-DRB1 genetic data.The preliminary explanations about the contradiction were given in this paper.     

客家人的红细胞血型分布

对父母双方上溯三代均为客家人的广东梅县200名 (其中男89人,女111人) 健康学生进行了红细胞血型ABO,MNSs,Rh,Kidd,Duffy,Diego,Xg,Lewis及P等系统的分布调查。结果显示,客家人的基因频率S=0.0250,NS=0,pl=0.0917和Fyb=0.0300,都是汉族人群中最低的。其它基因频率为r=0.6632,p=0.1863,q=0.1505;m=0.5250,n=0.4750,MS=0.0250,Ms=0.5000,Ns=0.4750,s=0.9750;C=0.6575,D=1.0000,E=0.1515,CDe=0.6226,cDE=0.1200,cDe=0.2189,CDE=0.0389;JKa=0.4642,JKb=0.4881,JK=0.0477;Fya=0.9700;Dia=0.0202,Dib=0.9798;Xga=0.3633,Xg=0.6367;P2=0.9083。发现了国内第二例Jk(a-b-)表型,未发现MNS型,NS型,NSs型,CCDEE型,CcDEE型,Fy(a-)型和Rho(-)型。Le(a+b-)型29人,Le(a+b+)型2人,Le(a-b+)型67人,Le(a-b-)型102人。客家人与国内19个群体的遗传距离计算结果表明,与客家人遗传距离最近的是福建汉族、湖南苗族、贵州汉族及广西侗族,其次为河南汉族、黑龙江汉族、陕西汉族,福建畲族及上海汉族,而与云南白族、辽宁满族、甘肃汉族、广西瑶族、广西壮族、内蒙汉族及四川彝族的遗传距离较远。与客家人遗传距离最远的是湖南土家族、海南苗族及海南黎族。     

Effects of Colchicine on Distribution of Mitochondria in 2-cell and Compacted 8-cell Mouse Embryos

The distribution of mitochondria during early development of mouse embryos was visualized bymitochondria-specific vital fluorescent dye, rhodamine 123(Rh 123). Mitochondrial clusters wasmarkedly conceotrated to perinuclear area in blastomere of normal 2-ccll embryos. In blastomere ofuncompacted 8-cell embryos, mitochondria were randomly distributed throughout the cytoplasm, butthey were reorganizcd to the cytocortices beneath the apposed surfaces of blastomere duringcompaction. As demonstrated in our study, colchicine (10 μg/ml) produced marked effect onmitochondrial distribution in blastomcre of 2-cell and compacted 8-cell embryos: mitochondriabecame scattered throughout the cytoplasm ofblastomere. It is suggested that the spatial distributionof mitochondria in early mouse embryo are maintained by microtubule.