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1.
By combining induced mutation, using NTG and UV irradiation, and protoplasting of a wild type strain of Aspergillus oryzae ATCC 22788, a hyper-producing strain was obtained that accumulated 41 g kojic acid l(-1) in shake-flasks, which was 100-fold higher than that in the wild type strains. Similar production of kojic acid was obtained in 5 l stirred-tank fermentations. 相似文献
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Kojic acid synthesis genes regulation was investigated in Aspergillus oryzae. Our results indicate that kojic acid production was lost in the laeA disruption strain, but was recovered in the LaeA complement strain. Real-time PCR also confirmed that expression of kojic acid biosynthesis genes decreased in the laeA disruption strain, indicating that these genes are under the control of LaeA. 相似文献
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Summary Membrane-surface liquid culture (MSLC) developed by the authors (Yasuhara et al., 1994) was applied to the production of kojic acid, using Aspergillus oryzae var.oryzae IFO 30113. By the fed-batch MSLC with intermittent glucose addition, the amount of kojic acid increased to over 50 times that obtained by means of the culture in shake flasks. 相似文献
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Repeated-batch production of kojic acid in a cell-retention fermenter using Aspergillus oryzae M3B9 总被引:1,自引:0,他引:1
Wan HM Chen CC Giridhar R Chang TS Wu WT 《Journal of industrial microbiology & biotechnology》2005,32(6):227-233
A cell-retention fermenter was used for the pilot-scale production of kojic acid using an improved strain of Aspergillus oryzae in repeated-batch fermentations. Among the various carbon and nitrogen sources used, sucrose and yeast extract promoted pellet morphology of fungi and higher kojic acid production. Repeated-batch culture using a medium replacement ratio of 75% gave a productivity of 5.3 g L–1 day–1 after 11.5 days of cultivation. While batch culture in shake-flasks resulted in a productivity of 5.1 g L–1 day–1, a productivity of 5 g L–1 day–1 was obtained in a pilot-scale fermenter. By converting the batch culture into repeated batches, the non-productive downtime of cleaning, filling and sterilizing the fermenter between each batch were eliminated, thereby increasing the kojic acid productivity. 相似文献
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Gui-ming Fu Ru-yi Li Kai-min Li Ming Hu Xiao-qiang Yuan Bin Li 《Preparative biochemistry & biotechnology》2016,46(8):780-787
This study aimed to obtain strains with high glyphosate-degrading ability and improve the ability of glyphosate degradation enzyme by the optimization of fermentation conditions. Spore from Aspergillus oryzae A-F02 was subjected to ultraviolet mutagenesis. Single-factor experiment and response surface methodology were used to optimize glyphosate degradation enzyme production from mutant strain by liquid-state fermentation. Four mutant strains were obtained and named as FUJX 001, FUJX 002, FUJX 003, and FUJX 004, in which FUJX 001 gave the highest total enzyme activity. Starch concentration at 0.56%, GP concentration at 1,370?mg/l, initial pH at 6.8, and temperature at 30°C were the optimum conditions for the improved glyphosate degradation endoenzyme production of A. oryzae FUJX 001. Under these conditions, the experimental endoenzyme activity was 784.15?U/100?ml fermentation liquor. The result (784.15?U/100?ml fermentation liquor) was approximately 14-fold higher than that of the original strain. The result highlights the potential of glyphosate degradation enzyme to degrade glyphosate. 相似文献
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Comparisons were made for alpha-galactosidase production using red gram plant waste (RGPW) with wheat bran (WB) and other locally available substrates using the fungus Aspergillus oryzae under solid-state fermentation (SSF). RGPW proved to be potential substrate for alpha-galactosidase production as it gave higher enzyme titers (3.4 U/g) compared to WB (2.7 U/g) and other substrates tested. Mixing WB with RGPW (1:1, w/w) resulted enhanced alpha-galactosidase yield. The volume of moistening agent in the ratio of 1:2 (w/v), pH 5.5 and 1 ml (1 x 10(6) spores) of inoculum volume and four days incubation were optimum for alpha-galactosidase production. Increase in substrate concentration (RGPW+WB) did not decrease enzyme yield in trays. 相似文献
8.
Kohtaro Kirimura Somsak Sarangbin Sugima Rugsaseel Shoji Usami 《Applied microbiology and biotechnology》1992,36(5):573-577
Summary Many mutant strains showing resistance to 2-deoxy-d-glucose (DG) on minimal medium containing glycerol as a carbon source were induced from Aspergillus niger WU-2223L, a citric acid-producing strain. The mutant strains were classifiable into two types according to their growth characteristics. On the agar plates containing glucose as a sole carbon source, mutant strains of the first type showed good growth irrespective of the presence or absence of DG. When cultivated in shake cultures, some strains of the first type, such as DGR1–2, showed faster glucose consumption and growth than strain WU-2223L. The period for citric acid production shortened from 9 days for strain WU-2223L to 6–7 days for these mutant strains. The levels and yields of citric acid production of the mutant strains were almost the same as those of strain WU-2223L. The mutant strains of the second type, however, showed very slow or no growth on both the agar plates containing glucose and fructose as sole carbon sources. In shake cultures, mutant strains such as DGR2-8 showed decreased glucose consumption rates, resulting in very low production of citric acid. 相似文献
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Summary Reports that starch is a poor substrate for lipid production are attributed to the low available C:N ratio which occurs because starch is not directly available to the microbial cell. If cultural conditions were established which gave rapid and extensive amylase activity, sufficient starch hydrolysis would occur to give a high ratio of available C:N, conditions favourable to lipid accumulation. This hypothesis was tested experimentally with Aspergillus oryzae and mycelium with 37% lipid content was obtained with starch as sole carbon source in defined culture media. 相似文献
10.
Fabio C. Chaves Thomas J. Gianfagna Madhu Aneja Francisco Posada Stephen W. Peterson Fernando E. Vega 《Mycological Progress》2012,11(1):263-267
Aspergillus oryzae NRRL 35191 was isolated as an endophyte from coffee leaves and found to produce kojic acid (KA) in culture. When inoculated
into cacao seedlings (Theobroma cacao), A. oryzae grew endophytically and synthesized KA in planta. Cacao seedlings inoculated with A. oryzae produced higher levels of caffeine than non-inoculated ones. Aspergillus oryzae may be a useful endophyte to introduce to cacao since it grows non-pathogenically and induces the caffeine defense response
that may make the plant more tolerant to insects and pathogens. 相似文献
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Molecular Biology Reports - A consistent kojic acid producing fungal strain has been isolated from rice husk using glucose-peptone medium. The isolate was identified as Aspergillus sojae SSC-3 on... 相似文献
13.
Conversion of acorn fringe extract into ellagic acid production by Aspergillus oryzae and Endomyces fibuliger were investigated. The results showed that ellagic acid production was maximized when co-fermentation of the two fungi was performed at 30 degrees C and pH 5.0 with 5.7 g/l of initial substrate concentration, which were close to the optimal values for both fungi to yield an appropriate consortium of hydrolytic enzymes. Meanwhile, it was found that the co-fermentation could compensate the deficiencies in the level of polyphenol oxidase activity from pure A. oryzae and the levels of ellagitannin acyl hydrolase and beta-glucosidase activities from pure E. fibuliger, resulting in. 0.91 g/l of biomass concentration containing 1.84 g/l of ellagic acid. The research not only demonstrates that the co-fermentation is an effective approach to utilize forest byproduct for ellagic acid production, but also provides more evidences for understanding evolution of ellagic acid production with enzymes actions, which is important for process control of ellagic acid production in industrial application. 相似文献
14.
Aspergillus niger strain CGU 87 was treated with UV radiation and some auxotrophic mutants were obtained. These mutants were less productive than CGU 87, which produced an average of 7.4% citric acid. All possible crosses in pair wise combinations were carried out between these auxotrophs, and three heterokaryons were synthesised. Finally, one heterozygous diploid was isolated from each of them. These heterokaryons and diploids showed improved productivity when compared with their component parents, but except in one diploid D5, all others produced less citric acid than CGU 87. The yield of D5 exceeded that of CGU 87 by 1.2 times and it produced 9% citric acid. This is a significant improvement and the increased productivity seems to be the result of successful adaptation of D5 to its fermentation environment. 相似文献
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Aflatoxin and cyclopiazonic acid production by a sclerotium-producing Aspergillus tamarii strain. 总被引:1,自引:0,他引:1
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The production of aflatoxins B1 and B2 by Aspergillus tamarii (subgenus Circumdati section Flavi) is reported for the first time. The fungus was isolated from soil collected from a tea (Camellia sinensis) field in Miyazaki Prefecture, Japan. Three single-spore cultures, NRRL 25517, NRRL 25518, and NRRL 25519, were derived from subcultures of the original isolate 19 (MZ2). Each of these single-spore cultures of A. tamarii produced aflatoxins B1 and B2 and cyclopiazonic acid, as well as black, pear-shaped sclerotia. The demonstration of aflatoxin production by A. tamarii is examined in connection with A. tamarii phylogenetic relationships, chemical ecology, and potential use in food fermentations. 相似文献
18.
Fang Xiaojiang Li Jian Zheng Xiaoyu Xi Yonglan Chen Kequan Wei Ping Ouyang PingKai Jiang Min 《World journal of microbiology & biotechnology》2011,27(12):3009-3013
Fermentation and succinic acid production by Actinobacillus succinogenes YZ0819 was inhibited by high NaCl. To enhance the resistance of this strain to osmotic stress, an NaCl-tolerant mutant strain
of A. succinogenes (CH050) was screened and selected through a continuous culture using survival in 0.7 M NaCl as the selection criterion. Using
Na2CO3 as the pH regulator and glucose as the carbon source in batch fermentation, the isolated osmo-resistant stain, A. succinogenes CH050, produced up to 66 g/l succinic acid with a yield of 73.37% (w/w). The concentration of succinic acid and mass yield
were increased by 37.5 and 4.37%, respectively, compared to the parent strain. The dry cell weight reached 10.1 g/l, which
is 37% higher than that of the parent strain. The high tolerance of A. succinogenes CH050 to osmotic stress increased improved the succinic acid production from batch fermentation. 相似文献
19.
Zhu Yuanting Li Jianlong Yao Kai Zhao Nan Zhou Kang Hu Xinjie Zou Likou Han Xinfeng Liu Aiping Liu Shuliang 《Applied microbiology and biotechnology》2016,100(22):9773-9786
Applied Microbiology and Biotechnology - A novel filamentous fungus M-4 strain was isolated from soy sauce koji and identified as Aspergillus oryzae (Collection number: CGMCC 11645) on the basis of... 相似文献
20.
Amino acid transport in spores of Aspergillus oryzae 总被引:1,自引:0,他引:1
H Sinoara 《Journal of biochemistry》1974,76(3):513-522