Observations of the structural changes that occur during charcoalification: implications for identifying charcoal in the fossil record

All of our current understanding of fossil charcoal structure comes from observations of modern wood charcoal produced in furnaces. These charcoals consistently show cell wall homogenization after prolonged heating (>325°C) and this is therefore considered to be a key identifying feature of fossil charcoal. Yet furnaces are unable to replicate the full combustion processes that occur during a wildfire. Here, for the first time, we have studied the microscopic structural evolution of charcoal produced using calorimetry, wherein the wood is ignited under controlled conditions and the heat release rate and other parameters measured, and the resulting charcoal studied using reflected light microscopy. We show that homogenization of cell walls is actually only a short‐lived phase of charcoal formation that occurs during the early heating stages as the pyrolysis front traverses through the wood. Cell wall homogenization is then rapidly overprinted by the thinning, distortion and breakdown of cell walls, and a notable visual increase in reflectance. Our preliminary study therefore suggests that we need to first improve our understanding of charcoal formation in order to better understand the fossil record of wildfires.     

First multi-proxy record of Jurassic wildfires from Gondwana: Evidence from the Middle Jurassic of the Neuquén Basin,Argentina

Wildfires play a crucial role in recent and ancient ecosystem modeling but their detailed history on the Earth is still not well recorded or understood. The co-occurrence of charcoal and pyrolytic polycyclic aromatic hydrocarbons (PAHs) is used for the recognition of wildfires in geological record that may have implications for the analysis of the terrestrial environment, ecosystems, climate and the level of atmospheric oxygen. Here we present the first multi-proxy evidence of wildfires on the Gondwana continent during the Jurassic, based on the occurrence of charcoal and pyrolytic PAHs in the Middle Jurassic of the Neuquén Basin, Argentina. This is the first evidence of wildfire in the Aalenian, the lowest stage of the Middle Jurassic, and one of the few records of wildfires in the Bathonian. Temperature interpretations, derived from charcoal reflectance data, show that charcoals formed in low temperature surface fires that only sporadically reached the higher temperatures, possibly related to crown fires. The occurrence of charcoals in the Middle Jurassic deposits confirms recent results that the atmospheric oxygen level reached at least 15% during the Middle Jurassic times.     

Latest Permian palynomorphs from Jharia Coalfield,Damodar Basin,India and their potential for biostratigraphic correlation

The objective of the present work is to palynologically date the samples collected from Jharia Coalfield. The samples have been collected from an outcrop section exposed across the Damodar river near NH 32 enroute from Bokaro to Dhanbad (23° 43′06.93″N and 86° 12′06.90″E) near Jharia Coalfield of Damodar Basin. Palynological analysis of the samples has revealed that the palynomorph assemblage is characterized by high incidence of Densipollenites spp. in association with dominance of striate bisaccates chiefly Striatopodocarpites spp. and Faunipollenites spp. The other stratigraphically significant taxa recorded in the section are Guttulapollenites hannonicus, Klausipollenites schaubergeri, Alisporites spp. Falcisporites spp. and Lunatisporites spp. On the basis of the palynocomposition the section has been assigned a latest Permian age. Age determination for the section has been inferred by comparison with coeval assemblages from previous Gondwana studies from India and across Gondwana which were compared to the biostratigraphically dated palynological records from Australia (oldest APP6 Zone) as it is one of the few areas in Gondwana where the palynological records have been calibrated against adequately dated marine invertebrate zones. However, comparison with the westernmost portion of Gondwana was difficult due to its mid latitudinal position (c. 20°S) during the Lopingian (late Permian) which lead to an early transition from coal-bearing strata to red-bed and evaporitic deposits in some parts.     

塔里木盆地北缘侏罗纪孢粉组合

本文系统地研究了塔里木盆地北缘侏罗纪的孢子花粉。共发现孢粉化石５２属９０种,以及藻类化石２属２种。建立侏罗纪的孢粉组合序列如下：（１）Ｄｉｓａｃｃｉａｔｒｉｌｅｔｉ－Ｃｙａｔｈｉｄｉｔｅｓ组合（早侏罗世早期阿合组）,（２）Ｃｙａｔｈｉｄｉｔｅｓ－Ｃｉｂｏｔｉｕｍｓｐｏｒａ－Ｄｉｓａｃｃｉａｔｒｉｌｅｔｉ组合（早侏罗世晚期阳霞组）,（３）Ｃｙａｔｈｉｄｉｔｅｓ－Ｎｅｏｒａｉｓｔｒｉｃｋｉａ－Ｄｉｓａｃｃｉａｔｒｉｌｅｔｉ组合（中侏罗世早期克孜勒努尔组）,（４）Ｃｙａｔｈｉｄｉｔｅｓ－Ｃｌａｓｏｐｏｌｉｓ组合（中侏罗世晚期恰克马克组）。根据孢粉组合特征讨论了地质时代,同时探讨了古植被和古气候的意义。     

木炭研究方法

木炭作为重要的植物遗存,是重建植被历史、恢复古气候、探讨早期人类活动的理想材料。准确识别木炭以及获取有代表性的样品是顺利开展研究工作的先决条件;木炭的定量研究可提高植被类型及其演替历史的研究精度;而其内部特殊结构组织的观察将提供更为丰富的信息。本文基于近年来国内外最新研究成果,对木炭的鉴别,样品的采集、鉴定和统计等进行系统的归纳和总结,并提出该指标研究过程中仍需解决的一些关键问题。     

Evidence of fungal decay in petrified legume wood from the Neogene of the Bengal Basin,India

Silicified fossil legume woods of Cynometroxylon Chowdhury & Ghosh collected from the Neogene (late Miocene) sediments of the Bengal Basin, eastern India, exhibit fungal decay seldom found in the fossil record. The wood possesses numerous perforate areas on the surface that seem to be the result of extensive fungal activity. In transverse section, the decayed areas (pockets) appear irregular to ellipsoidal in outline; in longitudinal section these areas of disrupted tissue are somewhat spindle-shaped. Individual pockets are randomly scattered throughout the secondary xylem or are restricted to a narrow zone. The aforesaid patterns of decay in fossil wood show similarities with that of white rot decay commonly produced by higher fungi, specifically basidiomycetes and ascomycetes. The host fossil wood harbors abundant ramifying and septate fungal hyphae with knob like swellings similar to pseudoclamps in basidiomycetes, and three-celled conidia-like reproductive structures. This record expands our current knowledge of wood decaying fungi-host plant interaction in the Neogene tropical forests of Peninsular India.     

A new species of Nelumbo from South China and its palaeoecological implications

A new species of Nelumbo Adanson is described from the Eocene of the Changchang Basin, Hainan Island, South China. This is the first comprehensive report on a fossil Nelumbo species from the Eocene of the Changchang Basin, Hainan Island. The co-occurrence of fruits, tubers, rhizomes with numerous adventitious roots, leaves, and receptacles indicates that the plants grew where they are preserved. These specimens, combined with palynological assemblage and other macrofossils, indicate a tropical or subtropical humid climate in the Changchang Basin from the late early Eocene to early late Eocene.     

塔里木盆地北缘三叠纪孢粉组合

研究区发现孢粉化石９０属１９７种,藻类化石２属４种,疑源类２属２种。建立三叠纪的孢粉组合序列,并详细地讨论了各个组合的地质时代。根据孢粉组合反映的植物群面貌,探讨盆地北缘三叠纪的古植被和古气候。     

Infrared video microscopy—an efficient-method for the routine investigation of opaque organic-walled microfossils

Summary Dark or even opaque organic-walled microfossils are frequently represented in palynological residues which are derived from sediments of high maturity. Such particles may be studied surficially by SEM or incident light, but internal details remain obscured. It has been known for some time that transparency may be achieved by applying infrared microscopy, but the method never became routine. We have now assembled an IR equipment which is based on a regular transmitted light microscope and tested our setup on various kinds of palyno-morphse.g. spores, acritarchs, chitinozoans, scolecodonts as well as phytoclasts and zooclasts. Our examples are from several localities of different stratigraphic and palaeogeographic position within the Palaeozoic. The studies revealed a high potential for IR video microscopy to become an important tool in the study of thermally altered organic particles in palynological slides.     

Vegetation changes during the Holocene in the North Ibersá, Corrientes, Argentina

Vegetation changes during the Holocene in the North Iberá, Corrientes, Argentina. Wetlands are very important sites for palynological studies, since they represent one of the most suitable environments for fossil pollen preservation. The aim of this work was to determine, by palynological analysis of lacustrine sediments, the vegetal communities and the predominant environment during the Holocene in NW of Iberá. Two lagoons were studied: San Sebastián and San Juan Poriahú. Sediment samples were obtained with witness using a "Levingstone square-rod sampler", processed with Faegri e Iversen techniques and dated with C14. The palynological graphs were divided in zones using the Tilia program. The palynological analysis allowed visualizing diverse changes in the vegetation: from 6 140 +/- 50 to 5 170 +/- 100 a. C., the NW of Iberá was characterized by marsh-herbaceous vegetation and arboreal vegetation typical of dry vegetation. From 5 170 +/- 100 to 3 460 +/- 60 a. C., a decrease in the species frequency, typical of wet environments, is produced, and the clogging of the waterbody, from 3460 +/- 60 a. C. onwards, while continuing the dominance of herbaceous vegetation typical of these environments, the arboreal pollen, indicates the beginning of a hygrophilous forest development.     

Late Carboniferous palynostratigraphy of the Itararé Subgroup, northeastern Paraná Basin, Brazil

A new biozonation is proposed for the Late Carboniferous strata of the northeastern Paraná Basin (São Paulo and Paraná States), based on well-preserved palynological assemblages recovered from outcrops and core samples retrieved from the Itararé Subgroup. Assemblages include forty-nine species of trilete spores and twenty-five pollen species. The oldest biozone, the Ahrensisporites cristatus Interval Zone (AcZ), in the basal portion of the Itararé Subgroup, includes eleven exclusive spore species, and is assigned a Pennsylvanian (late Bashkirian to Kasimovian) age. The overlying proposed biozone, the Crucisaccites monoletus Interval Zone (CmZ), dated late Pennsylvanian (Kasimovian to Gzhelian), ranges approximately from the top of the lower portion to the middle portion of the Itararé Subgroup, bears only one exclusive species of pollen. Both zones are characterized by the dominance of trilete spores and monosaccate pollen grains, including also few taeniate pollen grains. Furthermore, the basal Protohaploxypinus goraiensis Subzone of the Vittatina costabilis Interval Zone (VcZ) is broadly recognized for the first time in the upper portion of the Itararé Subgroup in the northeastern Paraná Basin. This subzone is attributed to the Early Permian (Early Cisuralian), and is characterized by the dominance of monosaccate pollen grains, a significant increase of taeniate grains (mainly Protohaploxypinus spp.), apart from the appearance of polyplicate pollen (Vittatina spp.). Reference sections and the main characteristics of the two new zones (AcZ and CmZ) are presented, along with a preliminary palynological correlation and discussion on their ages.     

Two Eocene fossil fruits from the Changchang Basin of Hainan Island,China

Two types of fossil fruit, one belonging to Palaeocarya sp. (Juglandaceae) and the other to Acer cf. A. miofranchetii Hu et Chaney (Aceraceae), are found in the Eocene coal-bearing series from the Changchang Basin of Hainan Island, China. This is the first fossil record of Palaeocarya and Acer in a tropical area of China. These fossils provide evidence for an investigation of the phytogeographic history of these two genera. Since their extant relative genera are distributed mostly in northern temperate or tropical–subtropical mountainous regions, I propose that the Changchang Basin of Hainan Island was close to a mountainous region in the Eocene; the plants bearing these fruits were growing at a mid-high altitude with a relatively cool climate, and the fruits were not preserved in situ but transported to the fossil site. The characters of other associated fossil plants and palynological data also support this hypothesis.     

Novel Application of Confocal Laser Scanning Microscopy and 3D Volume Rendering toward Improving the Resolution of the Fossil Record of Charcoal

Variations in the abundance of fossil charcoals between rocks and sediments are assumed to reflect changes in fire activity in Earth’s past. These variations in fire activity are often considered to be in response to environmental, ecological or climatic changes. The role that fire plays in feedbacks to such changes is becoming increasingly important to understand and highlights the need to create robust estimates of variations in fossil charcoal abundance. The majority of charcoal based fire reconstructions quantify the abundance of charcoal particles and do not consider the changes in the morphology of the individual particles that may have occurred due to fragmentation as part of their transport history. We have developed a novel application of confocal laser scanning microscopy coupled to image processing that enables the 3-dimensional reconstruction of individual charcoal particles. This method is able to measure the volume of both microfossil and mesofossil charcoal particles and allows the abundance of charcoal in a sample to be expressed as total volume of charcoal. The method further measures particle surface area and shape allowing both relationships between different size and shape metrics to be analysed and full consideration of variations in particle size and size sorting between different samples to be studied. We believe application of this new imaging approach could allow significant improvement in our ability to estimate variations in past fire activity using fossil charcoals.     

Synchronous Wildfire Activity Rise and Mire Deforestation at the Triassic–Jurassic Boundary

The end-Triassic mass extinction event (∼201.4 million years ago) caused major faunal and floral turnovers in both the marine and terrestrial realms. The biotic changes have been attributed to extreme greenhouse warming across the Triassic–Jurassic (T–J) boundary caused by massive release of carbon dioxide and/or methane related to extensive volcanism in the Central Atlantic Magmatic Province (CAMP), resulting in a more humid climate with increased storminess and lightning activity. Lightning strikes are considered the primary source of wildfires, producing charcoal, microscopically recognized as inertinite macerals. The presence of polycyclic aromatic hydrocarbons (PAHs) of pyrolytic origin and allochthonous charcoal in siliciclastic T–J boundary strata has suggested widespread wildfire activity at the time. We have investigated largely autochthonous coal and coaly beds across the T–J boundary in Sweden and Denmark. These beds consist of predominantly organic material from the in situ vegetation in the mires, and as the coaly beds represent a substantial period of time they are excellent environmental archives. We document a remarkable increase in inertinite content in the coal and coaly beds across the T–J boundary. We show estimated burning temperatures derived from inertinite reflectance measurements coupled with palynological data and conclude that pre-boundary late Rhaetian mire wildfires included high-temperature crown fires, whereas latest Rhaetian–Sinemurian mire wildfires were more frequent but dominated by lower temperature surface fires. Our results suggest a major change in the mire ecosystems across the T–J boundary from forested, conifer dominated mires to mires with a predominantly herbaceous and shrubby vegetation. Contrary to the overall regional vegetation for which onset of recovery commenced in the early Hettangian, the sensitive mire ecosystem remained affected during the Hettangian and did not start to recover until around the Hettangian–Sinemurian boundary. Decreasing inertinite content through the Lower Jurassic suggests that fire activity gradually resumed to considerable lower levels.     

Fossil charcoals from the Lower Jurassic challenge assumptions about charcoal morphology and identification

Charcoal morphometry is increasingly employed in the analysis of Quaternary sediments, but has not been applied in studies of earlier sediments. We present findings relating to charcoal morphometry and morphotypes in a Toarcian sequence, which contains a preponderance of highly elongate forms, and morphometric values well outside expected ranges. SEM imaging reveals elongate particles of two distinct kinds. One originates in conifer xylem, and may be associated with formation at high levels of heating. It is proposed that the other may derive from tree ferns, or from plants with similar growth forms. Our results show the importance of recognizing the wide morphological variation that exists in sedimentary charcoal. Failing to do so may result in serious errors in its identification, interpretation and quantification.     

Pre-Quaternary wood decay ‘caught in the act’ by fire – examples of plant-microbe-interactions preserved in charcoal from clastic sediments

The fossil record contains abundant evidence for the activity of microorganisms in the form of characteristic decay structures within fossil plant remains. Despite an abundance of charcoal in many sedimentary environments, there is little published evidence of such decay structures within charcoal from pre-Quaternary clastic deposits. The present contribution presents some examples of pre-Quaternary charcoal from clastic sediments which exhibit pre-charring decay structures, stratigraphically reaching from the Permian up to the Oligocene. Examples include specimens affected by the principle types of wood rot known from modern ecosystems (i.e. brown-rot, white rot and soft-rot) as well as a peculiar decay pattern resembling an atypical type of white-rot, which is only rarely known from modern wood. Theoretically there are different, so far hypothetical, scenarios which could be used to explain the scarcity of published reports on such material. Besides taphonomical biases directly influencing the sedimentary record of charcoal towards material not affected by microbial decay, it is conceivable that the lack of reports of such material from pre-Quaternary clastic deposits represents a, maybe unintentional, bias introduced by scientists working on pre-Quaternary charcoal.     

Cretaceous diversification of angiosperms in the western part of the Iberian Peninsula

The classic leaf fossil floras from the Cretaceous of the Lusitanian Basin, Portugal, which were first described more than one hundred years ago, have played an important role in the development of ideas on the early evolution of angiosperms. Insights into the nature of vegetational change in the Lusitanian Basin through the Cretaceous have also come from studies of fossil pollen and spores, but the discovery of a series of mesofossil floras containing well-preserved angiosperm reproductive structures has provided a new basis for understanding the systematic relationships and biology of angiosperms at several stratigraphic levels through the Cretaceous. In the earliest mesofossil floras from the Torres Vedras locality, which are of probable Late Barremian-Early Aptian age, angiosperms are surprisingly diverse with about 50 different taxa. In slightly later mesofossil floras, which are of probable Late Aptian-Early Albian age, the diversity of angiosperms is still more substantial with more than hundred different kinds of angiosperm reproductive structures recognized from the Famalicão locality alone. However, this early diversity is largely among angiosperm lineages that produced monoaperturate pollen (e.g., Chloranthaceae, Nymphaeales) and early diverging monocots (Alismatales). Eudicots are rare in these Early Cretaceous mesofossil floras, but already by the Late Cenomanian the vegetation of the western Iberian Peninsula is dominated by angiosperms belonging to various groups of core eudicots. The Normapolles complex is a particularly conspicuous element in both mesofossil floras and in palynological assemblages. In the Late Cretaceous mesofossil floras from Esgueira and Mira, which are of Campanian-Maastrichtian age, core eudicots are also floristically dominant and flowers show great organisational similarity to fossil flowers from other Late Cretaceous floras described from other localities in Asia, Europe and North America.     

Changes in the relative abundance of Polyarthra vulgaris and P. dolichoptera,following the elimination of fish

In the last decade palynological investigations of the sediments of large lakes in the USSR (Ladoga, Onega, Kubenskoye, Vozhe, Lacha, Beloye) and the Baltic Sea have been carried out. Limnic and marine sediments are notable for their high pollen and spore concentrations and pollen diagrams from these deposits have a regional significance. The diagrams from Lakes Onega and Ladoga belong to the Kola-Karelian type while the sediments of Lacha, Vozhe, Kubenskoye and Beloye belong to the North-Russian type (Neustadt 1957). Three types of Holocene pollen diagrams are registered for the Baltic Sea. Modern and fossil pollen spectra reflect the composition of the zonal vegetation. Late-glacial sediments are found in several profiles of lake and sea deposits. Holocene deposits are divided into eight stratigraphic zones: Late-glacial sediments (DR₂-Al-DR₃) and Post-glacial sediments (PB, BO, AT, SB, SA). The zonations of lake and sea sediments can be clearly correlated.     

Un sol de mangrove fossilise dans les lignites du Soissonnais (Ypresien) a Verzenay (Marne)

At Verzenay, in the Reims area, the upper part of the “Lignites du Soissonnais” have been turned into a paleosol. A petrographical and geochimical study of this profile allow us to see there a fossilized mangrove soil.A palynological study carried out on the same samples shows typical taxons of the mangrove environment (Nypa, Bruguiera, Acrostichum aureum). Then, palynological results perfectly agree with the sedimentological interpretation.     

First fossil evidence of Palaeocarya (Engelhardioideae: Juglandaceae) from India and its biogeographical implications

Even though presently indigenous to eastern Himalaya in India, no Engelhardioideae have been reported from the Cenozoic sediments of India till date. Here, we report the first Indian occurrence of a characteristic engelhardioid winged samaroid fruit having a tri-lobed wing (oblong-ovate median lobe and two lateral lobes) and a globose nut from the latest Neogene (Pliocene: Rajdanda Formation) sediments of Chotanagpur Plateau, eastern India. This is the first fossil evidence of relict family Juglandaceae from the Indian Cenozoic. We determine its taxonomic position on the basis of detailed macromorphological comparison with similar extant and fossil specimens and discuss its palaeoclimatic significance in terms of the present-day distribution of modern analogous species. We assign this Pliocene winged fruit specimen to the morphogenus Palaeocarya sect. Monocosta Manchester and describe it as a new species, namely Palaeocarya indica Hazra, Hazra M & Khan sp. nov. Palaeocarya sect. Monocosta has rich fossil records from the Cenozoic sediments of Europe, North America, and eastern Asia (China, Korea), but the modern analog, Engelhardia, is presently native only to India and neighboring Southeast Asia. We discuss the possible causes of disappearance of Engelhardia from the present-day vegetation of Chotanagpur Plateau. Its disappearance may be related to the gradual intensification of monsoonal rainfall seasonality since the Pliocene. Here, we also review in detail the biogeographic history of Palaeocarya sect. Monocosta and suggest its possible migration routes.