Developmental evolution: going beyond the 'just so'

Two new protocols for infecting non-mammalian embryos with viruses, together with RNA inhibition, have provided evolutionary developmental biologists with the tools to study the effects of manipulating gene activity in a wide range of species, allowing them to test hypotheses rather than rely on inference from similarity.     

Endophyte research: going beyond isolation and metabolite documentation

Many fungi belonging to mostly Ascomycota inhabit living tissues of plants of all major lineages without causing any visible symptoms. Termed horizontally transmitted endophytes, they have been investigated mostly for their capacity to produce bioactive secondary metabolites. However, many questions regarding the interactions between endophytes and their plant hosts, phytophagous insects and other fungi remain unanswered. This review highlights some of these areas of endophyte biology about which very little or no knowledge exists. Information garnered' using modern methodologies' on these grey areas of ‘endophytism’ (endophytic mode of lifestyle) would help immensely in understanding the evolution of endophytes of aerial plant tissues and in exploiting endophytes in various fields of biotechnology.     

Histone modifying enzymes and cancer: going beyond histones

Mutations in the molecular pathways that regulate cell proliferation, differentiation, and cell death all contribute to cancer formation. Enzymes that covalently modify histones affect these pathways by controlling the dynamic remodeling of chromatin structure. This article reviews several connections between histone modifying enzymes and cancer that are likely mediated via both histone and non-histone substrates. We propose that multiple protein modifications, including phosphorylation, methylation, and acetylation, cross regulate one another to coordinate intermolecular signaling, and that miscues in this regulation can lead to oncogenesis.     

Prospects for the genetic manipulation of dairy cattle: opportunities beyond BST

The dairy industry, with regulatory approvals of recombinant chymosin and bovine somatotropin (BST), has been at the forefront of food and agricultural biotechnology. The commercial fate of these products is one of several factors that may affect the success of future genetic manipulations in dairy cattle and dairy products. Other factors include technical and reproductive constraints in cattle and the cost of producing transgenic cattle. Early applications of genetic manipulation in cattle, for reasons of cost recoupment, may favor production of heterologous proteins in milk for pharmaceutical and medical use. Such applications could benefit genetic modification of milk and milk proteins for food use by providing advance knowledge and experience in mammalian protein expression. Other research opportunity areas that could affect prospects for genetic manipulation of dairy cattle include genome mapping, metabolic pathways, growth and development, and cattle/microbe interactions.     

Movement behaviour of woodland rodents: looking from beyond small trapping grids

Live-trapping of rodents was conducted over a 12-ha plot on unevenly spaced trap lines, with inner lines forming a 3.5-ha grid of closely spaced traps. This design was used to estimate the probability that bank volesClethrionomys glareolus (Schreber, 1780) and yellow-necked miceApodemus flavicollis (Melchior, 1834) trapped in small grids are true residents rather than visitors from the surrounding area. On average, 12% of the voles and 19% of the mice were trapped within and beyond the grid in the same trapping sessions. As these were mainly wide-ranging males moving over the plot, home ranges of males may be underestimated on small grids. In total, 36% of voles and 39% of mice that were marked in spring-summer were trapped at least once in their life beyond the grid. Typically, these were individuals shifting home ranges and migrating to or from the grid. The size of lifetime ranges of rodents was significantly larger than temporary home ranges and may therefore be underestimated on small grids. “Single--capture” individuals were mainly true transients rather than visitors. Only 12% of voles and 15% of mice resided on the plot for longer time than in the grid.     

Why going beyond standard LCI databases is important: lessons from a meta-analysis of potable water supply system LCAs

Purpose

Our aim is to assess the comparability and generic applicability of harmonized published lifecycle assessment (LCA) studies on water supply systems. In the absence of localized life cycle inventories for water systems, generic or country specific databases may be inadequate if applied elsewhere. The objectives of this paper are to calculate the potential magnitude of errors introduced by this practice and recommend ways to better account for sources of impact variability.

Methods

In this study, harmonization has been carried out rigorously, utilizing a systematic differentiation of the subsystems, functional units, and system boundaries referenced in over 100 candidate studies, resulting in a comparable subset of 34 LCA studies. Statistical techniques (cluster analysis and Welch’s analysis of variance) were used to isolate and validate the main sources of variation in impact scores and identify the sub-systems in which these are most pronounced. The significance of technology-specific contribution to the impacts was compared to the significance of electricity as a contributing factor to the global warming potential (GWP) by applying statistical correlation analysis.

Results and discussion

Our review revealed that most of the published LCAs analyzed water systems in well-developed countries. Large variation was found in the impacts of water supply systems (e.g., GWP between 0.16 and 3.4 kg CO2-eq/m³ of supplied water), with mean value of 0.84 kg CO2-eq/m3 and median of 0.57 kg CO2-eq/m3. The main contributor to GWP is water production and desalination in particular, making water production the most important differentiating factor. Cluster analysis also showed that production technology is the most important differentiating factor with respect to terrestrial acidification, ozone depletion, eutrophication, and abiotic depletion impacts of water production systems. There is a weak correlation between impact scores of electricity mixes and entire water supply systems.

Conclusions

An LCA of water-intensive products drawing from a standard life cycle inventory databases could be substantially inaccurate, especially in a region with desalination. More accurate results can be achieved by taking local water production technology into account. Meta-analysis is a useful tool to explore the sources of variance in the impacts of water systems. Applying harmonized results is a cost-effective way for obtaining more accurate LCA results as compared to applying generic databases only.

     

Differential effects of central serotonin manipulation on hyperactive and stereotyped behaviour

The locomotor response following injection of dopamine into the nucleus accumbens was attenuated by the injection of 5HT and potentiated by the injection of methysergide into the same site. D-amphetamine-induced locomotor activity was also reduced by the intra-accumbens injection of 5HT. In contrast, apomorphine- induced stereotyped behaviour (sniffing, licking, biting, gnawing) was reduced by systematic administration of the putative 5HT receptor antagonists, cyproheptadine and metergoline. In addition the low intensity sniffing responses produced by a low dose of apomorphine were converted to high intensity biting, gnawing or licking by the putative 5HT receptor agonist, quipazine or the putative 5HT uptake blocker, ORG 6582. The selective induction of either hyperactive or stereotyped behaviour may therefore be influenced by the functional state of central serotonergic systems.