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Soybean DREB1/CBF‐type transcription factors function in heat and drought as well as cold stress‐responsive gene expression
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Satoshi Kidokoro Keitaro Watanabe Teppei Ohori Takashi Moriwaki Kyonoshin Maruyama Junya Mizoi Nang Myint Phyu Sin Htwe Yasunari Fujita Sachiko Sekita Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《The Plant journal : for cell and molecular biology》2015,81(3):505-518
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Identification of Arabidopsis genic and non‐genic promoters by paired‐end sequencing of TSS tags
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Mutsutomo Tokizawa Kazutaka Kusunoki Hiroyuki Koyama Atsushi Kurotani Tetsuya Sakurai Yutaka Suzuki Tomoaki Sakamoto Tetsuya Kurata Yoshiharu Y. Yamamoto 《The Plant journal : for cell and molecular biology》2017,90(3):587-605
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Nataliya Kovalchuk Wei Jia Omid Eini Sarah Morran Tatiana Pyvovarenko Stephen Fletcher Natalia Bazanova John Harris Kontanze Beck‐Oldach Yuri Shavrukov Peter Langridge Sergiy Lopato 《Plant biotechnology journal》2013,11(6):659-670
Constitutive over‐expression of the TaDREB3 gene in barley improved frost tolerance of transgenic plants at the vegetative stage of plant development, but leads to stunted phenotypes and 3‐ to 6‐week delays in flowering compared to control plants. In this work, two cold‐inducible promoters with contrasting properties, the WRKY71 gene promoter from rice and the Cor39 gene promoter from durum wheat, were applied to optimize expression of TaDREB3. The aim of the work was to increase plant frost tolerance and to decrease or prevent negative developmental phenotypes observed during constitutive expression of TaDREB3. The OsWRKY71 and TdCor39 promoters had low‐to‐moderate basal activity and were activated by cold treatment in leaves, stems and developing spikes of transgenic barley and rice. Expression of the TaDREB3 gene, driven by either of the tested promoters, led to a significant improvement in frost tolerance. The presence of the functional TaDREB3 protein in transgenic plants was confirmed by the detection of strong up‐regulation of cold‐responsive target genes. The OsWRKY71 promoter–driven TaDREB3 provides stronger activation of the same target genes than the TdCor39 promoter. Analysis of the development of transgenic plants in the absence of stress revealed small or no differences in plant characteristics and grain yield compared with wild‐type plants. The WRKY71–TaDREB3 promoter–transgene combination appears to be a promising tool for the enhancement of cold and frost tolerance in crop plants but field evaluation will be needed to confirm that negative development phenotypes have been controlled. 相似文献
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M. Lorenzo M. L. Pinedo M. A. Equiza P. V. Fernndez M. Ciancia D. G. Ganem J. A. Tognetti 《Plant biology (Stuttgart, Germany)》2019,21(Z1):84-94
- Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature. These responses are associated with cold hardiness, but their underlying mechanisms remain largely unknown. Here we analyse the effects of low temperature on leaf morpho‐anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold‐hardening ability.
- A combined microscopy and biochemical approach was applied to study actively growing leaves of winter (ProINTA‐Pincén) and spring (Buck‐Patacón) wheat developed under constant warm (25 °C) or cool (5 °C) temperature.
- Cold‐grown plants had shorter leaves but longer inter‐stomatal epidermal cells than warm‐grown plants. They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose. These effects were more pronounced in the winter cultivar (Pincén). Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three‐fold increase in the distal mature zone of the leaf. This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C.
- The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold‐induced compact morphology and cold hardening. New insights are presented on the potential temperature‐driven role of peroxidases and hemicellulose in cell wall dynamics of grasses.
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中国仓鼠卵巢(Chinese hamsters ovary,CHO)细胞是目前重组蛋白质生产的首选宿主细胞。利用CHO细胞生产重组蛋白质,启动子是启动转基因转录的关键。核心启动子是RNA聚合酶与转录起始复合物集合的部位,分为集中型和分散型两种类型。目前,CHO细胞常用的启动子为病毒启动子、异源启动子、内源性和诱导性启动子等。也可以利用合成生物学及相关的数据库,人工设计合成启动子及鉴定新型启动子。本文综述了CHO细胞常用的启动子以及人工设计的合成启动子在CHO细胞中重组蛋白质表达方面的进展,为哺乳动物细胞选择合适的启动子,保证蛋白质表达量最大化,并确保长时间表达稳定性提供参考。 相似文献
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The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance 总被引:3,自引:0,他引:3
Xiaoming Sun Langlang Zhang Darren C. J. Wong Yi Wang Zhenfei Zhu Guangzhao Xu Qingfeng Wang Shaohua Li Zhenchang Liang Haiping Xin 《The Plant journal : for cell and molecular biology》2019,99(5):988-1002
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中国仓鼠卵巢(Chinese hamsters ovary,CHO)细胞是目前重组蛋白质生产的首选宿主细胞。利用CHO细胞生产重组蛋白质,启动子是启动转基因转录的关键。核心启动子是RNA聚合酶与转录起始复合物集合的部位,分为集中型和分散型两种类型。目前,CHO细胞常用的启动子为病毒启动子、异源启动子、内源性和诱导性启动子等。也可以利用合成生物学及相关的数据库,人工设计合成启动子及鉴定新型启动子。本文综述了CHO细胞常用的启动子以及人工设计的合成启动子在CHO细胞中重组蛋白质表达方面的进展,为哺乳动物细胞选择合适的启动子,保证蛋白质表达量最大化,并确保长时间表达稳定性提供参考。 相似文献
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Overexpression of a novel cold‐responsive transcript factor LcFIN1 from sheepgrass enhances tolerance to low temperature stress in transgenic plants
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Qiong Gao Xiaoxia Li Junting Jia Pincang Zhao Panpan Liu Zhujiang Liu Liangfa Ge Shuangyan Chen Dongmei Qi Bo Deng Byung‐Hyun Lee Gongshe Liu Liqin Cheng 《Plant biotechnology journal》2016,14(3):861-874
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Design of an optimal promoter involved in the heat‐induced transcriptional pathway in Arabidopsis,soybean, rice and maize
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Kyonoshin Maruyama Takuya Ogata Norihito Kanamori Kyouko Yoshiwara Shingo Goto Yoshiharu Y. Yamamoto Yuko Tokoro Chihiro Noda Yuta Takaki Hiroko Urawa Satoshi Iuchi Kaoru Urano Takuhiro Yoshida Tetsuya Sakurai Mikiko Kojima Hitoshi Sakakibara Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《The Plant journal : for cell and molecular biology》2017,89(4):671-680
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光、温度、水分等环境因素影响植物的生长发育,植物可以通过启动子中顺式作用元件与转录因子的相互协调作用,对这些信号产生响应,调控基因表达。本文综述了光、温度、水分诱导表达启动子中的顺式作用元件及相关转录因子研究的最新进展,从分子水平上探讨了环境因子诱导的基因表达调控,对研究植物适应环境的机制具有一定的意义。 相似文献