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1.
Total flavonoid content (TFC) and cyanidin‐3‐glucoside (Cyd‐3‐glu) of seed and seed coat extract of 16 genotypes from five species of Carthamus were studied, and their major polyphenolic compounds and antioxidant activity of the seed coat extracts were determined using HPLC analysis and DPPH assay, respectively. Additionally, fatty acids composition of the seed oil was analyzed by GC. In general, TFC and Cyd‐3‐glu content of seed coat extracts were higher than those of seed extracts. A novel breeding line with black seed coat (named A82) depicted lower TFC (3.79 mg QUE/g DW) but higher Cyd‐3‐glu (24.64 mg/g DW) compared to the white and other seed‐pigmented genotypes. DPPH radical scavenging activity showed a strong association with Cyd‐3‐glu content (r = 0.84), but no correlation with TFC (r = ?0.32). HPLC analysis of seed coat extracts revealed that four compounds were dominant constituents including rutin (7.23 – 117.95 mg/100 g DW), apigenin (4.37 – 64.88 mg/100 g DW), quercetin (3.09 – 14.10 mg/100 g DW), and ferulic acid (4.49 – 30.41 mg/100 g DW). Interestingly, the genotype A82 with an appropriate polyunsaturated/saturated fatty acids index (5.46%) and a moderate linoleic fatty acid content (64.70%) had higher nutritional and pharmaceutical value than all the other genotypes. 相似文献
2.
以野生百合渥丹、山丹和传统食用的兰州百合为研究对象,对其鳞茎中多酚类物质、11种单体酚的含量及抗氧化活性(ABTS自由基、超氧阴离子、羟自由基的清除能力,铜离子还原能力以及抑制脂质过氧化活性)进行了分析。结果表明:两种野生百合鳞茎中的多酚类物质含量及抗氧化活性均显著高于兰州百合。3种百合鳞茎中单体酚的种类也有所不同,但均含有没食子酸、矢车菊素-3-芸香糖苷、儿茶素、表儿茶素、杨梅酮、芦丁、对香豆酸、山奈酚。相关性分析显示,除对羟自由基的清除力外,各酚类物质总量与不同抗氧化指标之间呈显著正相关关系。试验结果认为,野生百合鳞茎可作为天然抗氧化资源应用于食品和医药业,具有一定的开发应用前景。 相似文献
3.
The chemical composition and in vitro antioxidant activity of the essential oil of propolis (EOP) collected from 25 locations in China was investigated. Steam‐distillation extraction was used to extract the EOP, and chemical composition was identified by GC/MS. The antioxidant activities of EOP were also measured. The result showed that a total of 406 compounds were detected in EOP. The major compounds of Chinese EOP were cedrol, γ‐eudesmol, benzyl alcohol, phenethyl alcohol, 2‐methoxy‐4‐vinylphenol, 3,4‐dimethoxystyrene and guaiol. Principal component analysis revealed the significant correlation between EOP compositions and their origins, and certain correlation was detected between EOP and their color. Linear discriminant analysis showed that 88 % and 84 % of the propolis samples were predicted correctly as the groupings identified by climatic zone and the color, respectively. Furthermore, the differences of antioxidant activities of EOP were significant. EOP of Shandong had the strongest antioxidant activities, whereas EOP of Guangdong, Yunnan and Hunan showed the poorest. 相似文献
4.
Loretta Pobłocka-Olech Daniel Głód Anna Jesionek Maria Łuczkiewicz Mirosława Krauze-Baranowska 《化学与生物多样性》2021,18(7):e2100227
The chemical composition in terms of flavonoid and salicylic compounds of leaves from 6 species and 3 hybrids of poplars (Populus) was identified with the use of TLC and HPLC-DAD/ESI-MS methods. Chromatographic analyses were carried out with 21 standard compounds including salicylic compounds (2), phenolic acids (3) and flavonoids (16). Moreover, on the basis of the obtained chromatographic data from the HPLC-DAD/ESI-MS and TLC separations, the presence of salicortin, tremulacin and chlorogenic acid was confirmed, depending on the analyzed poplar species or hybrid. The content of salicylic compounds was determined by HPLC-UV method and expressed on salicin as free and total fraction. Total flavonoid content was determined by spectroscopic method as quercetin equivalent. Significant qualitative and quantitative differences in the chemical composition of the analyzed leaves were demonstrated. The highest concentration of flavonoids (8.02 mg/g) was found in the leaves of Populus nigra, while the highest content of salicylic compounds (47.14 mg/g) was found in the leaves of P.×berolinensis. The antioxidant and xanthine oxidase inhibition properties of extracts from poplar leaves were investigated by TLC bioautography. It has been shown that the richest set of compounds with antioxidant properties are present in the leaves of P. alba, P.×candicans and P. nigra. 相似文献
5.
为了解蕈树叶芳香精油化学组分及抗氧化活性,采用气相色谱-质谱联用技术(GC-MS)结合Kovats保留指数(K I)比较的方法对其进行了成分分析,并运用二种体外方法对其抗氧化活性进行了测定。结果表明蕈树叶精油以倍半萜烯类为主(占62.39%),主要特征成分为双环大根香叶烯(10.71%)、(E)-丁香烯(9.96%)和α-依兰油烯(8.92%)。该精油具有中等程度的自由基清除活性和抗脂质过氧化活性。其抗氧化活性可能与精油中的酚类物质5-羟基白菖莆烯(2.97%)以及醇类物质1-表橙椒醇(3.12%)和(Z)-白檀油烯醇(2.12%)等化合物有关。 相似文献
6.
Patrizia Monica Mastino Mauro Marchetti Jean Costa Claudia Juliano Marianna Usai 《化学与生物多样性》2021,18(6):e2100053
The Cistaceae family is well represented in Sardinia, and the Cistus genus is widely used in traditional medicine. Nowadays only few studies have been performed on this genus vegetating in Sardinia in spite of its ethnobotanical importance. Moreover, in the past there have been conflicting opinions among botanists for the exact assignment of the species growing in Sardinia. We started several years ago to carried out studies on this genus and in the present study was to evaluate the in vitro activity of several samples of Cistus salvifolius L., Cistus monspeliensis L., and Cistus albidus L. collected in Sardinia as antimicrobial agents against Escherichia coli, Staphylococcus aureus, and four Candida species and their antioxidant activity using DPPH, ABTS, and FRAP assays. Furthermore, the phenolic content and composition of the extracts were first evaluated. Using statistical multivariate analysis on the complete metabolomics profile of all Cistus species growing wild in Sardinia, we confirmed the botanical classification, and we observed an interesting correlation between metabolomics profile and antioxidant activity. 相似文献
7.
采用高温水提工艺、低温水提工艺和微波辅助工艺从软枣猕猴桃中提取得到三种多糖,依次命名为AAP-1、AAP-2和AAP-3,对三种多糖的理化性质、单糖组成和抗氧化活性进行了研究.理化性质鉴定结果表明:三种多糖均不含酚羟基及还原糖,都含有一定量的糖醛酸和蛋白质,AAP-1含有淀粉,AAP-2和AAP-3不含淀粉;单糖组成结果表明:三种多糖均由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖7种单糖组成,其中,AAP-1葡萄糖的摩尔百分含量最高,为94.72%;AAP-2半乳糖和阿拉伯糖的摩尔百分含量较高,分别为24.75%、38.37%;AAP-3半乳糖醛酸的摩尔百分含量较高,为16.05%.抗氧化实验结果表明:AAP-1抗氧化活性最弱;AAP-3抗氧化活性最强,其清除DPPH自由基和羟基自由基的IC50分别为1.2、2.7 mg/mL. 相似文献
8.
Hacer Tugba Degirmencioglu Etil Guzelmeric Parla Isil Yuksel Hasan Krmzbekmez Inci Deniz Erdem Yesilada 《化学与生物多样性》2019,16(12)
This study was undertaken to analyze the phenolic profiles of 19 propolis samples from Turkey by using a high‐performance thin‐layer chromatographic (HPTLC) method in order to identify their plant origins. Furthermore, their antioxidant and antimicrobial activity profiles were comparatively evaluated. For the appraisal of antioxidant potential, total phenolic (TPC) and total flavonoid contents (TFC) of propolis samples were firstly determined and then their effects on free radicals were evaluated by FRAP, ABTS.+, CUPRAC, DPPH. and HPTLC‐DPPH. methods. Antimicrobial activity of propolis samples against Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 15442), Escherichia coli (ATCC 11229) and Candida albicans ATCC 10231 were determined by disc diffusion and broth dilution methods. HPTLC fingerprinting analyses revealed that O‐type (botanical origin from Populus nigra L.) was the primarily available propolis type in Turkey. Moreover, 3‐O‐methylquercetin (3MQ) rich propolis was identified as a new propolis type for the first time. Principal component analysis (PCA) indicated that 3MQ‐type propolis differs from the O‐type. Antioxidant activity studies showed that O‐type of propolis possesses higher antioxidant effect than the other tested propolis types. Quercetin, caffeic acid, caffeic acid phenethyl ester (CAPE) and galangin were determined to contribute significantly to the antioxidant potential of O‐type propolis among others. Propolis extracts exerted moderate antimicrobial activity against the tested microorganisms with MIC values between the ranges of 128–512 μg/mL. 相似文献
9.
New norcantharidin analogs were designed and obtained as compounds with biological activity. As a starting material, exo‐7‐oxabicyclo[2.2.1]heptane‐2,3‐dicarboxylic acid anhydride was used. Three groups of compounds: dicarboximides, triazoles and thiazolidines were obtained in multistep reactions. The 1H‐ and 13C‐NMR spectra were used to confirm the structures of all obtained products and they were in agreement with the proposed structure of substances. All derivatives were screened for their antioxidant activity. The most promising group was dicarboximides ( 1 – 4 , 6 ). Derivatives 2–4 displayed antioxidant activity with EC50=7.75–10.89 μg/ml, which may be comparable to strong antioxidant Trolox (EC50=6.13 μg/ml). Excellent activity with EC50=10.75 μg/ml also presented norcantharidin analog with 1,2,4‐triazole system ( 12 ). 相似文献
10.
Leila Ghorbani Dr. Pooran Golkar Dr. Reza Jafari Dr. Mohsen Ahmadi Dr. Alireza Allafchian 《化学与生物多样性》2023,20(9):e202300811
This study evaluated several secondary metabolites, essential oils (EOs) compositions, and antioxidant activity in four medicinal plants that originated in Isfahan rangelands. The species were Astragalus verus, Astragalus adscendens, Daphne mucronata, and Phlomis olivieri. Thirty-two genotypes of these species were evaluated for different biochemical traits. Based on the evaluation of EOs compounds, GC/MS analysis revealed the total number of identified compounds. These compounds were 25, 22, 12, and 22 for A. adscendens, A. verus, D. mucronata, and P. olivieri, respectively. The dominant compounds were phthalate (59.88 %) in A. adscendens, phytol (38.02 %) in A. verus, hexanoic acid (32.05 %) in D. mucronata and β-cubebene (30.94 %) in P. olivieri. Phytochemical analysis showed that D. mucronata, A. adscendens, and P. olivieri had the highest total phenolics content (TPC) (18.24 mg gallic acid equivalent/g dry weight), total flavonoids content (5.57 mg QE/g DW), and total anthocyanins content (0.23 mg/g DW), respectively. The highest total chlorophyll (0.27 mg/g DW), total carotenoids (0.03 mg/g DW), and antioxidant activity (71.36 %) were observed in A. adscendens, A. adscendens and A. verus, respectively. Among all genotypes, the highest TPC (20.1 mg GAE/g DW) was observed in genotype 5 of D. mucronata. This study provided new information on the chemical compounds within the distribution range of these ecologically dominant rangeland species in Isfahan province, Iran. The data revealed that superior genotypes from these species are rich in natural antioxidants and bioactive compounds. Thus, they can be used in ethno pharmacological fields, food, and industrial applications. 相似文献
11.
低聚壳聚糖经醚化得到三种取代度不同的N,O-羧甲基壳聚糖(NOA、NOB和NOC),本文对其结构进行表征,考察了其对超氧阴离子自由基O2、DPPH自由基和过氧化氢(H2O2)的清除活性和还原能力。结果表明NOA、NOB和NOC对O2和DPPH的清除活性随-OH位置取代度的降低而升高;对H2O2的清除率随-NH2位置取代度的降低而升高;还原能力大小随其总取代度的升高而增强。 相似文献
12.
Essential oil (EO) composition, phenolic content, and antioxidant activity were investigated in 17 P. abrotanoides populations collected from different geographical regions in Iran. The highest (3.61%) and lowest (1.25%) essential oil yields were measured in populations from Semnan Province (PSESM2) and PISKS from Isfahan Province, respectively. GC/MS analysis identified camphor (4.05 – 35.94%), 1,8‐cineole (7.15 – 24.34%), borneol (0 – 21.75%), and α‐pinene (2.05 – 10.33%) as the main constituents of Perovskia essential oil. Cluster analysis classified the studied populations into four different groups: (I) high camphene, (II) high camphor/1,8‐cineole, (III) high borneol/δ‐3‐carene, and (IV) high α‐cadinol/trans‐caryophyllene. The highest flavonoid and phenolic contents were detected in PISAK from Isfahan Province (4.09 ± 0.05 mgQE/gDW, 58.51 ± 1.63 mgGAE/gDW) and PKRGS from Khorasan Province (3.80 ± 0.002 mgQE/gDW, 66.86 ± 0.002 mgGAE/gDW). DPPH and reducing power activity model systems identified PMASA and PKRKL as the populations with the highest antioxidant activity. Finally, the data obtained represented valuable information for introducing elite populations with EO components favorable to pharmaceutical and industrial applications. 相似文献
13.
Andrei V. Bogdanov Kamilla R. Iskhakova Alexandra D. Voloshina Anastasia S. Sapunova Natalia V. Kulik Natalia V. Terekhova Maxim V. Arsenyev Guzel K. Ziyatdinova Sergey V. Bukharov 《化学与生物多样性》2020,17(5)
The increase in the resistance of pathogens, in particular Staphylococcus aureus, to the action of antibiotics necessitates the search for new readily available and non‐toxic drugs. In solving this problem, phenolic acylhydrazones have high potential. In this communication, the synthesis of quaternary ammonium compounds containing a differently substituted phenolic moiety has been performed. An initial study of antimicrobial activity showed that these compounds are highly selective against S. aureus and B. cereus. The highest activity (MIC 2.0 μm ) was shown by hydrazones containing a catechol fragment. These compounds are more than 3‐fold more active against S. aureus and 3–10‐fold more active against B. cereus than norfloxacin. Low hemolytic and high antioxidant activities of all new compounds were also established. 相似文献
14.
The chemical components and antioxidant activity of 16 Rehmannia glutinosa samples were investigated to reveal the high‐quality raw resource for pharmaceutical products. 22 main chemical components were detected with significant content differences (P<0.05). The contents of 14 substances reached the maximum in S1 sample such as catalpol (6.74 mg g?1), rehmaionoside A (1.93 mg g?1) and rehmannioside D (5.13 mg g?1). However, the content distribution of the other eight substances had no obvious change regulation. Three antioxidant evaluation methods commonly showed that S1 sample had strong antioxidant activity with a low IC50 value of 0.022 mg mL?1, a high ABTS value of 524.196 μmol equiv. Trolox g?1, and a high FRAP value of 200.517 μmol equiv. Trolox g?1. Considered the medicinal value, S1 had high quality based on the present phytochemical profiles and antioxidant activity. These results also indicated that the root extracts of R. glutinosa could become useful supplement for pharmaceutical products as new antioxidant agents. 相似文献
15.
Milena Rašeta Mira Popović Ivana Beara Filip Šibul Gokhan Zengin Sanja Krstić Maja Karaman 《化学与生物多样性》2021,18(2):e2000828
The aim of this research work was to study the bioactivity potentials (antioxidant, anti-inflammatory, and enzyme inhibitory) of ethanol (EtOH), water (H2O) and chloroform (CHCl3) extracts of G. applanatum, G. lucidum, G. pfeifferi and G. resinaceum as well as their mycochemical profile: the total content of phenolics (TP) and sugars (TS) and LC/MS/MS detection of phenolics. LC/MS/MS profile showed that p-hydroxybenzoic and protocatechuic acids were mostly found. The highest ABTS and DPPH activities were detected in polar G. applanatum extracts (159.84±0.59 mg TE/g d.w., IC50=0.85±0.30 μg/mL, respectively), while G. resinaceum CHCl3 extract was the most potent in NO assay (IC50=41.21±0.18−81.89±0.81 μg/mL). The highest TP and TS were generally determined in G. applanatum EtOH extracts. Enzyme inhibitory effects were determined in H2O extracts. Generally, CHCl3 extracts showed the most powerful anti-inflammatory potential. These results suggest that analyzed species are a promising source of bioactive compounds and may be considered as candidates for new food supplements or drug formulations. 相似文献
16.
本文以新鲜的金桂花(Osmanthus fragrans Lour)为实验材料,采用微波-蒸馏萃取法提取桂花中的精油。通过改变微波-蒸馏萃取法中的各种工艺参数(匀浆时间、料液比、微波时间),得到相应工艺参数下的桂花精油提取率。采用单因素试验和正交试验,分析提取工艺过程中的参数和提取率得到最优化的桂花精油提取工艺条件,即匀浆时间4min、料液比1∶5、微波时间60s,同时桂花精油提取率为0.85%。根据对桂花精油的理化性质分析可知,实验所得桂花精油样品香气较为纯正,液体呈橘黄色,酸值大约为7.50,酯值大约为18.03,达到精油的国标标准且质量较好。对桂花精油采用磷钼络合物法进行抗氧化性的研究,表明桂花精油的抗氧化活性较强,同时桂花精油的浓度与抗氧化性成正比关系。 相似文献
17.
茉莉花渣多糖含量测定方法的建立及抗氧化作用研究 总被引:1,自引:0,他引:1
建立了茉莉花渣多糖含量的测定方法,并对茉莉花渣多糖进行了抗氧化作用研究,结果表明:采用蒽酮-硫酸比色法以624 nm为测定波长在质量浓度为10~60 mg.L-1(R2=0.9993)范围内与吸光度呈良好线性关系,该法的平均加样回收率为100.17%,重复性良好(RSD=0.57%),测定准确可靠。茉莉花渣多糖在0.2~1.0 g.L-1范围内对.OH具有明显的清除作用,IC50为0.52 g.L-1。 相似文献
18.
Variation in Phenolic Content,Profile, and Antioxidant Activity of Seeds among Different Paeonia ostii Cultivated Populations in China
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The purpose of this study was to analyze the phenolic profiles of seeds from fifteen Paeonia ostii cultivated populations in China and identify their relationship with antioxidant activities and associated environmental factors. Thirteen individual phenolic compounds were quantitatively determined by HPLC, and (+)‐catechin was the most abundant phenolic compound in the seeds. Correlation analysis showed that phenolics were the most effective antioxidant compound class by evaluating DPPH, ABTS, and hydroxyl radical scavenging activities as well as ferric reducing antioxidant power. Latitude and annual rainfall had significant effects on the contents of many phenolic compounds, and elevation was only significantly correlated with gallic acid content. Within fifteen P. ostii cultivated populations, the seeds of Tongling population exhibited the highest phenolic contents and strongest antioxidant activities. These results suggest that Tongling population has a relatively high utilization value and a potential for sources of natural antioxidants. 相似文献
19.
Supercritical carbon dioxide (SC-CO2), hydrodistillation (HDO), ethanol extraction (EE), and petroleum ether extraction (PE) were used to extract the essential oil and extracts of Cinnamomum camphora fruit in this study. Gas chromatography-mass spectrometry was used to identify the volatile components of essential oils and extracts, and 63 compounds were identified. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay and Ferric reducing ability of plasma (FRAP) assays and the inhibition experiment of bacteria and fungi (Staphylococcus aureus (S. aureus), Hay bacillus (H. bacillus), Escherichia coli (E. coli), Aspergillus niger (A. niger), Candida albicans (C. albicans)) showed these essential oils and extracts indicated antioxidant and antibacterial activities. S. aureus was the most sensitive to the essential oil (MIC=0.08 mg/ml). Combined with the Brine Shrimp Lethality Test (BSLT) experiment, HDO (LD50=68.21 μg/ml) was considered to have the most potential natural preservative. Subsequently, the inhibitory mechanism of HDO on bacteria and fungi was explored through extracellular conductivity and SEM, and the possibility of HDO to preserve the freshness of bananas was verified through banana shelf-life experiments. The results suggested these essential oils and extracts of Cinnamomum camphora fruit indicated effectively inhibit the growth of microorganisms on the surface of bananas, extend the shelf-life, and have the potential to become a natural antiseptic ingredient. 相似文献