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1.
A new species of Docosia Winnertz from Central Europe,with DNA barcoding based on four gene markers (Diptera,Mycetophilidae) 总被引:1,自引:0,他引:1
A new species of Docosia Winnertz, Docosia
dentata
sp. n., is described and illustrated, based on a single male specimen collected in Muránska planina National Park in Central Slovakia. DNA sequences (COI, COII, CytB, and ITS2) are included and compared for 13 species of Docosia. There was found only little congruence between the molecular results and previous scarce data about interspecific relationships based on morphology. The COI and CytB gene markers showed the highest interspecific gene distances while ITS2 showed the lowest ones. An updated key to the 23 Central European species of Docosia is also presented. 相似文献
2.
Two new Mycetophilidae species, Neuratelia
jabalmoussae
sp. n. and Neuratelia
salmelai
sp. n. are described on the basis of material collected from Lebanon, Estonia and Finland. Detailed figures of male terminalia and photographs of general facies are provided along with discussions of their morphological distinction from sibling species. For the first time molecular characters are used to distinguish new fungus gnat species. Molecular analysis relies on cytochrome oxidase subunit one (COI) but has additionally been corroborated by information from the 28S and ITS2 regions of nuclear ribosomal DNA. Situations where morphological and molecular data provide conflicting evidence for species delimitation are discussed. A new country record from Georgia is provided for Neuratelia
caucasica. 相似文献
3.
Nineteen species of abundant gastropods were collected at Robben Island, including introduced dune snails and European brown garden snails. They were identified using morphology and DNA barcoding. It was expected that the species recorded would be similar to those from the Cape peninsula, South Africa, but we were surprised to find some exceptions: the very abundant invasive mussel species in South Africa, the South American bisexual mussel (Semimytilus
algosus), and the beaded topshells (Oxystele
impervia) were not found on Robben Island. Possible explanations are presented for these differences. 相似文献
4.
Newly obtained and previously published sequences of the cytochrome c oxidase subunit I (COI) gene were analyzed to examine the biogeographic assembly of the caenogastropod fauna (belonging to the families Assimineidae, Cochliopidae, and Hydrobiidae) of an isolated spring along the lower Colorado River in southern Nevada (Blue Point Spring). Based on available COI clock calibrations, the three lineages that comprise this fauna are 2.78–1.42 million years old, which is roughly coeval or slightly younger than the age of Blue Point Spring (inferred from local fossil spring deposits). Two of the lineages—endemic Pyrgulopsis
coloradensis and Assiminea
aff.
infima—are most closely related to snails in the Death Valley area (well to the west) and likely colonized Blue Point Spring by transport on birds. A single haplotype was detected in both of these snails, suggesting that they may have only recently colonized Blue Point Spring. The third lineage—endemic Tryonia
infernalis, newly described herein based on morphological and molecular evidence—is most closely related to a geographically proximal species in a lower Colorado River tributary (Tryonia
clathrata); the split between these taxa may be the product of vicariance (severance of a prior drainage connection) or a separate jump dispersal event. The considerable genetic diversity in Tryonia
infernalis (three haplotypes differing by 0.6% mean sequence divergence) suggests a possibly lengthy history of local differentiation. Our findings also identify Blue Point Spring as a new micro-hotspot of groundwater-dependent biodiversity in Nevada and will assist ongoing efforts to protect and conserve these imperiled ecosystems. 相似文献
5.
Three species of the genus Xenocerogria Merkl, 2007 have been recorded in China, Xenocerogria
feai (Borchmann, 1911), Xenocerogria
ignota (Borchmann, 1941) and Xenocerogria
ruficollis (Borchmann, 1912). Xenocera
xanthisma Chen, 2002 is proposed as a junior synonym of Xenocerogria
ruficollis. Lectotype of Xenocerogria
ignota is designated, and the species is transferred to the genus Lagria Fabricius, 1775. New Chinese province records of Xenocerogria
ruficollis are provided. 相似文献
6.
7.
Takafumi Nakano 《ZooKeys》2014,(445):57-76
A new quadrannulate species of Orobdella, Orobdella
masaakikuroiwai
sp. n., from the mountainous region of central Honshu, Japan is described. This is only the second small species known within this genus, with a body length of less than 4 cm for mature individuals. Phylogenetic analyses using nuclear 18S rDNA and histone H3 as well as mitochondrial COI, tRNACys, tRNAMet, 12S, tRNAVal, 16S, and ND1 markers showed that Orobdella
masaakikuroiwai
sp. n. is the sister species of the quadrannulate Orobdella
whitmani Oka, 1895. Phylogenetic relationships within Orobdella
masaakikuroiwai
sp. n. conducted using mitochondrial markers reveled a distinction between eastern and western phylogroups. 相似文献
8.
Aegista
subchinensis (Möllendorff, 1884) is a widely distributed land snail species with morphological variation and endemic to Taiwan. Three genetic markers (partial sequence of the mitochondrial cytochrome c oxidase subunit I [COI], the 16S rDNA and the nuclear internal transcribed spacer 2 [ITS2]) were analysed to infer phylogenetic relationships and genetic divergence of closely related species of the genus Aegista, Aegista
vermis (Reeve, 1852) and Aegista
oculus (Pfeiffer, 1850). A new species from Aegista
subchinensis has been recognized on the basis of phylogenetic and morphological evidences. The nominal new species, Aegista
diversifamilia
sp. n. is distinguished from Aegista
subchinensis (Möllendorff, 1884) by its larger shell size, aperture and apex angle; wider umbilicus and flatter shell shape. The northernmost distribution of Aegista
diversifamilia
sp. n. is limited by the Lanyang River, which is presumed to mark the geographic barrier between Aegista
diversifamilia
sp. n. and Aegista
subchinensis. 相似文献
9.
John W. Brown 《ZooKeys》2014,(462):125-134
Circanota, new genus, and its two new species, Circanota
undulata
sp. n. (type species), from Costa Rica and Panama, and Circanota
simplex
sp. n., from Panama and Ecuador, are described and illustrated. Although superficially similar to some species of Platynota Clemens, 1860, Circanota appears to be more closely related to members of a putative clade within Sparganothini defined by a slender crescent-shaped signum in the corpus bursae of the female genitalia. The most conspicuous autapomorphy for Circanota is the strongly undulate costa of the male and female forewing. Barcode sequence data (i.e., cytochrome oxidase I) from Circanota
undulata (n = 12) form a tight cluster with exceedingly limited genetic divergence (less than 0.1%); specimens of Circanota
simplex have not been sequenced. In neighbor-joining trees based on COI, Circanota is portrayed nearest Sparganothoides, which is consistent with morphological evidence. 相似文献
10.
With 1,400 described species, Megaselia is one of the most species-rich genera in the animal kingdom, and at the same time one of the least studied. An important obstacle to taxonomic progress is the lack of knowledge concerning the phylogenetic structure within the genus. Classification of Megaselia at the level of subgenus is incomplete although Schmitz addressed several groups of species in a series of monographs published from 1956 to 1981. Another problem is the lack of molecular phylogenetic analyses to support morphology-based conclusions. As a contribution towards addressing these problems, we here circumscribe a previously unrecognized monophyletic lineage of Megaselia consisting of species similar to Megaselia
lucifrons. We base this taxonomic decision on morphological study of an extensive phorid material from Sweden, complemented by molecular analyses of select exemplars using two markers (COI and 28S). We name the clade the lucifrons species group, and show that it contains three distinct species. Our results also demonstrate that Megaselia
subnitida Lundbeck, 1920, previously treated as a synonym of Megaselia
lucifrons Schmitz, 1918, is a separate species, and we remove it from synonymy. The third species in the group was previously unknown; we describe it here as Megaselia
albalucifrons
sp. n. 相似文献
11.
Based on material collected from Pseudemys
nelsoni (Reptilia: Chelonia: Emydidae) during a parasite survey of the herpetofauna around Gainesville, Florida, USA, Polystomoides
nelsoni
sp. n. is described as a new polystome species. This parasite was found in the oral and pharyngeal region of the host. In a sample of nine Pseudemys
nelsoni, three specimens were found to release polystome eggs. One turtle was euthanized and dissected and found to be infected in the oral region with 19 specimens belonging to an as-yet-unknown Polystomoides. This is only the fifth Polystomoides recorded from the Nearctic realm. This species is distinguished from known species by a combination of characteristics including marginal hooklet morphology, body length and haptor dimensions. 相似文献
12.
13.
In most freshwater ecosystems, aquatic insects are dominant in terms of diversity; however, there is a disproportionately low number of records of alien species when compared to other freshwater organisms. The Chironomidae is one aquatic insect family that includes some examples of alien species around the world. During a study on aquatic insects in Amazonas state (Brazil), we collected specimens of Chironomidae that are similar, at the morphological level, to Chironomus
kiiensis Tokunaga and Chironomus
striatipennis Kieffer, both with distributions restricted to Asia. The objectives of this study were to provide morphological information on this Chironomus population, to investigate its identity using DNA barcoding and, to provide bionomic information about this species. Chironomus DNA barcode data were obtained from GenBank and Barcode of Life Data Systems (BOLD) and, together with our data, were analyzed using the neighbor-joining method with 1000 bootstrap replicates and the genetic distances were estimated using the Kimura-2-parameter. At the morphological level, the Brazilian population cannot be distinguished either from Chironomus
striatipennis or Chironomus
kiiensis, configuring a species complex but, at the molecular level our studied population is placed in a clade together with Chironomus
striatipennis, from South Korea. Bionomic characteristics of the Brazilian Chironomus population differ from the ones of Chironomus
kiiensis from Japan, the only species in this species complex with bionomic information available. The Brazilian Chironomus population has a smaller size, the double of the number of eggs and inhabits oligotrophic water, in artificial container. In the molecular analysis, populations of Chironomus
striatipennis and Chironomus
kiiensis are placed in a clade, formed by two groups: Group A (which includes populations from both named species, from different Asiatic regions and our Brazilian population) and Group B (with populations of Chironomus
kiiensis from Japan and South Korea). Genetic distance between the Brazilian population and specimens in Group A suggests that it was recently introduced in Brazil, and that its country of origin is probably South Korea. 相似文献
14.
The stalked barnacle Oxynaspis
gracilis, the chirostylid squat lobster Uroptychus sp., and the caridean shrimps Periclimenes
cf.
antipathophilus and Pseudopontonides
principis have been collected at 68–124 m by a remotely operated vehicle (ROV) on banks in the northern Gulf of Mexico. These species inhabited six species of antipatharian hosts. Pseudopontonides
principis, Oxynaspis
gracilis, and Uroptychus sp. were not confined to a single host species. Except for Oxynaspis
gracilis, collected by ROV in 2004–2005, these species have not been reported previously in the northwestern Gulf of Mexico. 相似文献
15.
The diverse genus Ophion is almost entirely undescribed in the Nearctic region. In this paper we define the Ophion
scutellaris species group. This species group is well-supported by analysis of DNA (ITS2, COI, and 28S D2-D3) and morphology. It includes the Palearctic species Ophion
scutellaris and the Nearctic species Ophion
idoneus. An integrative analysis of DNA, geometric wing morphometrics, classical morphometrics and qualitative morphology indicates that this species group contains a minimum of seven species in North America, although the full diversity of the group has likely not been sampled. Ophion
clave Schwarzfeld, sp. n., Ophion
aureus Schwarzfeld, sp. n., Ophion
brevipunctatus Schwarzfeld, sp. n., Ophion
dombroskii Schwarzfeld, sp. n., Ophion
keala Schwarzfeld, sp. n. and Ophion
importunus Schwarzfeld, sp. n. are described, and a key to the known Nearctic species of the Ophion
scutellaris group is provided. 相似文献
16.
The taxonomy of Kessleria, a highly specialized montane genus of Yponomeutidae with larval host restriction to Saxifragaceae and Celastraceae (Saxifraga spp. – subgenus Kessleria; Saxifraga spp. and Parnassia spp. – subgenus Hofmannia), is revised based on external morphology, genitalia and DNA barcodes. An integrative taxonomic approach supports the existence of 29 species in Europe (the two known species from Asia and North America are not treated herein). A full 658 bp fragment of COI was obtained from 135 specimens representing 24 species, a further seven sequences are >560 bp. Five new species are described: Kessleria
cottiensis
sp. n. (Prov. Torino, Italy; Dep. Hautes Alpes, France), Kessleria
dimorpha
sp. n. (Dep. Alpes-de-Haute-Provence, France), Kessleria
alpmaritimae
sp. n. (Dep. Alpes-Maritimes, France), Kessleria
apenninica
sp. n. (Prov. Rieti, Prov. L´Aquila, Italy), and Kessleria
orobiae
sp. n. (Prov. Bergamo, Italy). 相似文献
17.
The Afrotropical Cynipoidea are represented by 306 described species and 54 genera in four families: Cynipidae, Figitidae, Liopteridae and Ibaliidae, the latter represented by a single introduced species. Seven of these genera are only represented by undescribed species in the region. Seven new genus-level synonymies, one genus resurrected from synonymy, 54 new combinations, one combination reinstated, and one new replacement name are presented. We provide identification keys to the families, subfamilies and genera of cynipoid wasps occurring in the Afrotropical region (Africa south of the Sahara, including Madagascar and southern Arabian Peninsula). Online interactive Lucid Phoenix and Lucid matrix keys are available at: http://www.waspweb.org/Cynipoidea/Keys/index.htm. An overview of the biology and checklists of species for each genus are provided. This paper constitutes the first contributory chapter to the book on Afrotropical Hymenoptera. 相似文献
18.
Bruce D. Sutton Gary J. Steck Allen L. Norrbom Erick J. Rodriguez Pratibha Srivastava Norma Nolazco Alvarado Fredy Colque Erick Yábar Landa Juan José Lagrava Sánchez Elizabeth Quisberth Emilio Arévalo Pe?aranda P. A. Rodriguez Clavijo Jeniffer K. Alvarez-Baca Tito Guevara Zapata Patricio Ponce 《ZooKeys》2015,(540):175-191
The nuclear ribosomal internal transcribed spacer 1 (ITS1) was sequenced for Anastrepha
fraterculus (Wiedemann, 1830) originating from 85 collections from the northern and central Andean countries of South America including Argentina (Tucumán), Bolivia, Perú, Ecuador, Colombia, and Venezuela. The ITS1 regions of additional specimens (17 collections) from Central America (México, Guatemala, Costa Rica, and Panamá), Brazil, Caribbean Colombia, and coastal Venezuela were sequenced and together with published sequences (Paraguay) provided context for interpretation. A total of six ITS1 sequence variants were recognized in the Andean region comprising four groups. Type I predominates in the southernmost range of Anastrepha
fraterculus. Type II predominates in its northernmost range. In the central and northern Andes, the geographic distributions overlap and interdigitate with a strong elevational effect. A discussion of relationships between observed ITS1 types and morphometric types is included. 相似文献
19.
Jan Klimaszewski Reginald P. Webster Caroline Bourdon Georges Pelletier Benoit Godin David W. Langor 《ZooKeys》2015,(487):111-139
Six species of the genus Mocyta Mulsant & Rey are reported from Canada: Mocyta
amblystegii (Brundin), Mocyta
breviuscula (Mäklin), Mocyta
discreta (Casey), Mocyta
fungi (Gravenhorst), Mocyta
luteola (Erichson), and Mocyta
sphagnorum Klimaszewski & Webster, sp. n. New provincial and state records include: Mocyta
breviuscula – Saskatchewan and Oregon; Mocyta
discreta – Quebec, Ontario and Saskatchewan; Mocyta
luteola – New Brunswick, Quebec, Ontario, Massachusetts and Minnesota; and Mocyta
fungi – Saskatchewan. Mocyta
sphagnorum is described from eastern Canada from specimens captured in Newfoundland, New Brunswick, Quebec and Ontario. Mocyta
negligens Mulsant and Rey, a native European species suspected of occurring in Canada, is excluded from the Nearctic fauna based on comparison of European types with similarly coloured Canadian specimens, which are now identified as Mocyta
luteola. The European species, Mocyta
gilvicollis (Scheerpeltz), is synonymized with another European nominal species, Mocyta
negligens, based on examination of type material of the two species. Lectotypes are designated for Eurypronota
discreta Casey, Atheta
gilvicollis Scheerpeltz, Homalota
luteola Erichson, Colpodota
negligens Mulsant and Rey, Acrotona
prudens Casey and Dolosota
redundans Casey. The latter species is here synonymized with Mocyta
luteola. A review of the six Nearctic species is provided, including keys to species and closely related genera, colour habitus images, images of genitalia, biological information and maps of their distributions in Canada. 相似文献
20.
Males of certain species belonging to the Bactrocera
dorsalis complex are strongly attracted to, and readily feed on methyl eugenol (ME), a plant secondary compound that is found in over 480 plant species worldwide. Amongst those species is one of the world’s most severe fruit pests the Oriental fruit fly, Bactrocera
dorsalis
s.s., and the former taxonomic species Bactrocera
invadens, Bactrocera
papayae and Bactrocera
philippinensis. The latter species have been recently synonymised with Bactrocera
dorsalis based on their very similar morphology, mating compatibility, molecular genetics and identical sex pheromones following consumption of ME. Previous studies have shown that male fruit fly responsiveness to lures is a unique phenomenon that is dose species-specific, besides showing a close correlation to sexual maturity attainment. This led us to use ME sensitivity as a behavioural parameter to test if Bactrocera
dorsalis and the three former taxonomic species had similar sensitivity towards odours of ME. Using Probit analysis, we estimated the median dose of ME required to elicit species’ positive response in 50% of each population tested (ED50). ED50 values were compared between Bactrocera
dorsalis and the former species. Our results showed no significant differences between Bactrocera
dorsalis
s.s., and the former Bactrocera
invadens, Bactrocera
papayae and Bactrocera
philippinensis in their response to ME. We consider that the Bactrocera males’ sensitivity to ME may be a useful behavioural parameter for species delimitation and, in addition to other integrative taxonomic tools used, provides further supportive evidence that the four taxa belong to one and the same biological species, Bactrocera
dorsalis. 相似文献