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Zelda R. Althaus Jon M. Rowland James P. Freeman William Slikker 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1982,227(1):11-23
A high-performance liquid chromatography (HPLC) technique was developed for the determination of radiolabeled triamcinolone acetonide (TAC), cortisol and their metabolites in rhesus monkey plasma, urine and tissue samples. After protein precipitation, the parent compounds and metabolites were simultaneously resolved using a single-column reversed-phase HPLC system. TAC was subsequently verified by mass spectrometry and TAC glucuronide was tentatively identified by enzymatic hydrolysis and mass spectrometry of the hydrolysis product. The endogenous hormones, cortisol and cortisone were presumptively identified by cochromatography with authentic standards on two different HPLC systems and positively identified by reverse-isotope recrystallization. Other metabolites of both compounds were detected by selective enzymatic hydrolysis and HPLC. This method is rapid and reproducible with a total recovery > 80%. 相似文献
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P X Callahan J A Shepard T J Reilly J K McDonald S Ellis 《Analytical biochemistry》1970,38(2):330-356
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BROWN F 《The Biochemical journal》1950,47(5):598-600