共查询到20条相似文献,搜索用时 31 毫秒
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Hiroyoshi Kuzuhara Keiko Yakabi Hiroshi Ohrui Sakae Emoto 《Bioscience, biotechnology, and biochemistry》2013,77(2):285-288
The synthesis of (±)-epilupinine from trans-1-cyanoquinolizidines (IVa) and (IVb), the intermediates of the (±)-lamprolobine synthesis is described. 相似文献
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Lech Celewicz Wojciech Urjasz Krzysztof Golankiewicz 《Nucleosides, nucleotides & nucleic acids》2013,32(9):951-966
Abstract A series of 3′-N-substituted 3′-amino-3′-deoxythymidine derivatives with alkyl, alkenyl and alkylaryl substituents was synthesized by two methods. The first method involved the reaction of 1-(2,3-dideoxy-3-0-mesyl-5-0-trityl-β-D-threo-pentofuranosyl)thymine with an appropriate amine. In the second method, 3′-amino-5′-0-trityl-3′-deoxy-thymidine served as a synthetic precursor which was reacted with an appropiate aldehyde or ketone followed by sodium borohydride reduction. An improved synthesis of 3′-amino-3′-deoxythymidine from 3′ -azido-5′-0-trityl-3′-deoxythymidine using sodium borohydride was also described. 相似文献
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14-3-3蛋白研究进展 总被引:8,自引:1,他引:7
14-3-3蛋白是高度保守的、所有真核生物细胞中都普遍存在的、在大多数生物物种中由一个基因家族编码的一类蛋白调控家族。它几乎参与生命体所有的生理反应过程,人们在各种组织细胞中发现了各种不同的14-3-3蛋白。作为与磷酸丝氨酸/苏氨酸结合的第一信号分子,14-3-3蛋白在细胞的信号转导中起着至关重要的作用,尤其是它直接参与调节蛋白激酶和蛋白磷酸化酶的活性,被称为蛋白质与蛋白质相互作用的”桥梁蛋白”;它可以与转录因子结合形成复合体,调节相关基因的表达。一些研究表明,14-3-3蛋白调控机制的紊乱可以直接导致疾病的发生,在临床上14-3-3蛋白常常可以作为诊断的标志物。 相似文献
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问 :感冒了为什么会发热 ?答 :正常人的体温在大脑皮层和丘脑下部体温中枢的调节下保持相对的恒定 ,这主要是通过神经和体液等因素来调节产热和散热速度来实现的。如果产热和散热失去了动态平衡导致体温超出正常范围 ,则称为发热。人的体温一般维持在 3 7℃左右 (口腔温度范围为3 6.3℃ 3 7.2℃ ,腋下温度为 3 6℃ 3 7℃ ,直肠内温度为 3 6.5℃ 3 7.7℃ ) ,通常不会因外界温度的变化而变化。而一旦体温超过 3 9℃ ,则为高热 ,高热持续时间超过两周 ,则称长期高热 ,会严重影响人体的正常生理活动。最常引起人体发热的因素是致热源。与人体发… 相似文献
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Brock TG 《Neurochemical research》2008,33(5):801-807
Polyunsaturated fatty acids, like arachidonic acid, can bind proteins and affect their function. The 14-3-3 proteins bind
phosphorylated sites on a diverse array of client proteins and, in this way, are involved in many intracellular signaling
pathways. In this study, we used a novel approach to discover that 14-3-3ζ is able to directly bind arachidonic acid. Furthermore,
arachidonic acid, at physiological concentrations, reduced the binding of 14-3-3ζ to phosphorylated BAD, an interaction that
is important in regulating apoptosis. In addition, high concentrations of arachidonic acid caused the polymerization of 14-3-3ζ,
an event observed in neurodegenerative disorders. Taken together, these results indicate that arachidonic acid directly interacts
with 14-3-3ζ and that this interaction may be important in both normal and pathological cellular events. If so, then factors
that mediate the release, metabolism and reacylation of arachidonic acid into membranes represent key points of regulation. 相似文献
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14-3-3信号蛋白质家族是一组高度保守,分布十分广泛的多功能真核生物蛋白质,具有7个亚型,与各种信号肽分子包括激酶、磷酸酶、膜转移受体等结构,参与细胞内信号传导包括有丝分裂信号转导、细胞周期调节、细胞凋亡等,并对朊蛋白病有重要诊断价值。 相似文献
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Two-dimensional gel electrophoretic (NEPHGE) analysis of proteins from mouse 3T3B and 3T3B/SV40 cells labelled with [methyl-3H]methionine in the presence of cycloheximide have revealed that the elongation factor 1α (EF-1α) in these cells is methylated and that the extent of methylation is higher in the SV40 transformed cell type. It is suggested that methylation may account for differences in growth properties for the different cell types. 相似文献
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G. Negrón B. Quiclet-Sire Y. Diaz R. Gaviño R. Cruz 《Nucleosides, nucleotides & nucleic acids》2013,32(7):1539-1543
Abstract The preparation of 3-alkyl D4T derivatives has been carried out starting from the corresponding 5′-O-t-butyldimethylsilyl-3′-O-methanesulfonylthymidine 2 by way of deprotection-elimination and succesive alkylation reactions. 相似文献
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长链非编码RNA (long non-coding RNA, lncRNA)是一类长度大于200nt、没有长开放阅读框架但往往具有mRNA结构特征的RNA,可以在转录及转录后水平参与基因的表达调控。近年来,有研究证实lncRNA对脂肪生成具有重要作用。Lnc-RAP3位于小鼠(Mus musculus)17号染色体,其表达量在小鼠脂肪细胞分化前后呈现显著差异,但其具体的生物学功能尚不清楚。为探讨lnc-RAP3在小鼠3T3-L1前脂肪细胞成脂分化中的作用,本文首先构建了lnc-RAP3的真核表达载体pcDNA3.1-RAP3,利用脂质体将pcDNA3.1-RAP3和人工合成的lnc-RAP3的siRNAs分别转染3T3-L1前脂肪细胞,并对转染后的细胞进行诱导分化,并通过油红O染色、qRT-PCR检测成脂分化相关基因表达等方法比较过表达和敲降lnc-RAP3对3T3-L1前脂肪细胞成脂分化的影响。结果显示,过表达lnc-RAP3后,细胞内脂滴聚集显著减少(P<0.05),在诱导分化第0 d、2 d和4 d时C/EBPα、Glut4、PPARγ、LPL和FAS的表达水平均呈显著(P<0.05)或极显著(P<0.01)下降;敲降lnc-RAP3后,细胞内脂滴聚集显著增多(P<0.05),同时在诱导分化第0 d、2 d时PPARγ、LPL、C/EBPα、FAS和Glut4的表达水平呈显著(P<0.05)或极显著(P<0.01)升高。本研究结果表明,lnc-RAP3可能通过影响成脂分化相关基因的表达来抑制3T3-L1前脂肪细胞的成脂分化。 相似文献
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Anthonie J. van Essen Klaas Kok Anke van den Berg Bauke de Jong Femmy Stellink Arend F. Bos Hans Scheffer Charles H. C. M. Buys 《Human genetics》1991,87(2):151-154
Summary We report a girl with a de novo duplication of the distal part of the long arm of chromosome 3 and review the literature. Our patient had the facial characteristics and many other anomalies of the partial 3q duplication syndrome. As a hitherto undescribed symptom in partial 3q trisomy syndrome, she had microphthalmia. The karyotype of this girl was interpreted as an inverse duplication of the terminal portion of chromosome 3: 46,XX,inv dup (3)(pter-q28::q28–q25::q28-qter). Quantitative hybridisation studies with 3p and 3q probes gave a consistent 32 ratio of the relative intensities of the q bands in relation to the p bands between patient and control. This confirmed the presence of a 3q duplication and delineated the location of D3S5 to 3q25–3q28. 相似文献
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Seung R. Paik Ken Yokoyama Masasuke Yoshida Toshiko Ohta Yasuo Kagawa William S. Allison 《Journal of bioenergetics and biomembranes》1993,25(6):679-684
The ATPase activity of the F1-ATPase from the thermophilic bacterium PS3 is stimulated at concentrations of rhodamine 6G up to about 10 µM where 70% stimulation is observed at 36°C. Half maximal stimulation is observed at about 3 µM dye. At rhodamine 6G concentrations greater than 10 µM, ATPase activity declines with 50% inhibition observed at about 75 µM dye. The ATPase activities of the 33 and 33 complexes assembled from isolated subunits of TF1 expressed inE. coli deleted of theunc operon respond to increasing concentrations of rhodamine 6G nearly identically to the response of TF1. In contrast, the ATPase activities of the 33 and 33 complexes are only inhibited by rhodamine 6G with 50% inhibition observed, respectively, at 35 and 75 µM dye at 36°C. The ATPase activity of TF1 is stimulated up to 4-fold by the neutral detergent, LDAO. In the presence of stimulating concentrations of LDAO, the ATPase activity of TF1 is no longer stimulated by rhodamine 6G, but rather, it is inhibited with 50% inhibition observed at about 30 µM dye at 30°C. One interpretation of these results is that binding of rhodamine 6G to a high-affinity site on TF1 stimulates ATPase activity and unmasks a low-affinity, inhibitory site for the dye which is also exposed by LDAO. 相似文献
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目的 研究前列腺癌组织及前列腺癌细胞株PC- 3 中STAT3 蛋白及磷酸化STAT3 蛋白的表达。方法 常规石蜡包埋切片SABC免疫组化法检测45例前列腺癌组织、20例前列腺增生组织中STAT3 及磷酸化STAT3 表达, 细胞免疫化学法检测前列腺癌细胞株PC 3细胞STAT3及磷酸化STAT3表达。结果 STAT3在前列腺癌及前列腺增生组织表达阳性率分别为77. 8%和50. 0%, 两者间具有显著差异; 磷酸化STAT3在前列腺癌及前列腺增生组织表达阳性率分别为68. 9%和35. 0%, 两者间具显著差异(P<0 .05); PC- 3细胞中STAT3及磷酸化STAT3表达阳性。结论 STAT3蛋白在前列腺癌中高表达且持续激活, 可能与前列腺癌的发生具有密切联系。 相似文献
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Pozuelo-Rubio M 《Autophagy》2011,7(2):240-242
Autophagy is an evolutionarily conserved pathway involved in a great variety of physiological roles such as starvation adaptation, elimination of microorganisms, and intracellular protein and organelle clearance. It seems clear that autophagy is strictly controlled because of the multiplicity of its functions and thus, autophagy components are finely regulated. Here, 14-3-3ζ protein has been shown as negative regulator of autophagy by association and inactivation of the vesicle nucleation component vacuolar protein sorting 34 (hVps34), the class III phosphatidylinositol-3- kinase (PI3KC3). 相似文献
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水稻重复序列RRD3在CHO和3T3细胞中的启动子活性 总被引:3,自引:0,他引:3
RRD3是通过变性 -复性动力学从水稻基因组中克隆到的一个中度重复序列 .将 RRD3作为启动子亚克隆到氯霉素乙酰基转移酶基因为报告基因的 p CAT载体上 ,转染 5种动物细胞 .结果表明在 CHO、3T3细胞中能够启动 CAT基因的表达 .利用核酸外切酶 和 PCR构建了 RRD3的系列缺失体 ,经转染后的 CAT活性分析表明 ,该序列中存在多个与哺乳动物细胞中启动基因表达有关的调控元件 .结合 RRD3在大肠杆菌和高等植物烟草中启动报告基因表达的活性 ,对重复序列与生物进化的关系进行了讨论 . 相似文献