基于生态地理分区的大兴安岭植被物候时空变化

植被与气候的关系十分密切,植被物候可作为全球气候变化的指示器.大兴安岭位于我国最北部,对气候变化较为敏感,研究该区植被物候的时空变化对评估全球变化对陆地生态系统的影响具有重要意义.依据中国生态地理区划图,将大兴安岭划分为4个生态研究区域,本文利用GIMMS NDVI 3g遥感数据集分析1982—2012年大兴安岭整体及各生态地理分区植被物候变化.结果表明: 研究期间,所有分区植被生长季开始日期均表现为提前趋势,生长季结束日期均表现为推迟趋势.植被物候对气候因子变化敏感,尤其是对气温的敏感程度高于降水,其中,北段山地落叶针叶林区植被生长季开始日期与春季温度呈显著负相关;除南段草原区外,其他3个分区植被生长季结束日期均与秋季降水呈显著负相关.从整体来看,植被物候随海拔、纬度的变化趋势明显.     

基于SPOT NDVI的甘肃河东植被覆盖变化及其对气候因子的响应

基于1998—2011年SPOT NDVI和年均气温、降水数据,采用植被覆盖度法、趋势分析法及相关分析法对甘肃河东地区植被覆盖时空变化及其对气温和降水的关系进行研究。结果表明:河东地区植被覆盖度南高北低,依次为陇南山区甘南高原陇东高原陇中高原;近14年来植被覆盖度呈波动上升趋势,增速分别为陇南山区0.050 10 a-1、甘南高原0.009 10 a-1、陇东高原0.039 10 a-1、陇中高原0.023 10 a-1;植被覆盖轻度改善面积占37.20%,基本不变占59.75%,表明研究区植被覆盖稳中有所上升;河东地区植被NDVI与气温和降水的相关性各异,其中54.59%的地区植被NDVI与气温呈负相关,45.41%区域呈正相关;87.86%的地区植被NDVI与降水呈正相关,12.14%区域呈负相关;植被NDVI与降水正相关面积明显大于气温,说明降水是研究区植被生长的主导气候因子。     

22年来西北不同类型植被NDVI变化与气候因子的关系

 为了研究气候变化对西北地区不同类型植被的影响,利用NASA GIMMS 1982～2003年逐月归一化植被指数（Normalized difference vegetation index, NDVI）数据集和西北地区138个气象站点同期的气温和降水资料,计算了各站22年月平均气温和降水与NDVI的相关系数。同时, 选西北 地区森林、草原、绿洲和雨养农业4类有代表性的植被类型为研究区,对各类植被NDVI与气温和降水的相关关系进行分析。研究结果表明：除无 植被的戈壁沙漠地区外,西北地区NDVI与气温和降水均有较好的相关性。除祁连山中部地区外,西北地区NDVI与气温的相关系数大于降水。天 山、阿尔泰山和秦岭的NDVI与气温相关系数最高,而青海东北部NDVI与降水相关系数最高。西北地区各种类型植被对气候变化反映敏感。其敏 感度因植被类型不同和同类植被所处的地理位置不同而有差异;纬度较高的新疆林区与温度相关性最高,高寒草甸次之。在植被生长最旺盛的 夏季（6～8月）,22年来西北地区各林区的NDVI均呈下降趋势。其中西北东部林区下降趋势显著,与这些地区的降水减少和气温增加有关。草 原区植被以上升趋势为主,高寒草甸和盐生草甸上升趋势最为显著,气温升高是这些地区植被生长加速的原因 之一。西北绿洲是NDVI增加极为 显著的地区,以新疆绿洲NDVI上升趋势最大。气候变暖是近年绿洲NDVI增加的主要驱动力之一,绿洲面积扩大、种植结构调整和种植品种变化 等人为因素对绿洲NDVI增加的作用不可忽视,这种作用在新疆表现的尤为突出。雨养农业区NDVI年际 间波动较大,各区域间变化不太一致。 NDVI的波动与降水变化有很好的正相关,与气温变化有很好的负相关,近年来西北东部气温升高和降水的减少是雨养农业区NDVI下降的原因, 农业措施的实施也改变了植被生长对气候条件的依赖性。     

气候变化背景下京津风沙源区人类活动对植被影响的量化分析

在气候变化背景下,将人类活动对植被的影响进行量化研究,对于区域生态管理具有重要的现实意义。本研究基于GIMMS NDVI3g数据、气象数据(气温、降水)和标准化降水蒸散指数(SPEI),运用相关分析和趋势分析,研究京津风沙源区1982—2014年不同时期的植被时空变化及其驱动因子,再利用回归分析和残差分析定量研究了不同时期不同亚区人类活动对植被变化的影响。结果表明: 1982—2014年,京津风沙源区77.1%的植被退化状况得到明显改善,64.1%的地区植被生长呈上升趋势,年均NDVI呈现东南向西北递减的趋势。不同时期植被动态研究显示,74.5%的地区在京津风沙源治理工程实施后植被增加,最明显的是晋北山地丘陵亚区。在气候因子中,降水与植被变化的相关性最强;生态工程等人类活动在京津风沙源区的大部分地区起到了积极的作用,尤其是晋北山地丘陵亚区达到了94.9%。     

西南地区不同植被类型归一化植被指数与气候因子的相关分析

基于中国西南地区1982—2006年的归一化植被指数(NDVI)遥感数据集和气象数据,运用GIS技术对年均气温、年降水量和干旱指数进行插值,分析了西南地区不同植被类型(沼泽、灌丛、草丛、草原、草甸、针叶林、阔叶林、高山植被、栽培植被)NDVI的年际变化及其与气候因子的相关性.结果表明:研究期间,西南地区NDVI、年均气温、年降水量总体呈上升趋势,其中,年均气温的上升趋势达极显著水平,干旱指数则呈下降趋势;在9种植被类型中,沼泽和草丛NDVI呈下降趋势,且草丛的下降趋势达显著水平,其他7种植被类型的NDVI均呈上升趋势,且针叶林、草甸和高山植被的NDVI上升趋势达显著水平,灌丛NDVI呈极显著上升趋势.9种植被类型所在地区的年均气温均显著上升;年降水量的变化均不显著;沼泽、草丛和栽培植被所在地区的干旱指数呈上升趋势,草甸和高山植被所在地区的干旱指数显著下降,其他4种植被类型所在地区的干旱指数呈不明显的下降趋势.研究区灌丛和针叶林NDVI与年均气温呈显著正相关,灌丛和草甸NDVI与干旱指数呈显著负相关.在保持其他2个气候因子不变的情况下,针叶林、阔叶林、高山植被NDVI与年均气温的相关性最大,草丛NDVI与年降水...     

黄河上游不同干湿气候区植被对气候变化的响应

 研究气候变化背景下植被变化趋势及其与水热因子的关系, 对于黄河源区的生态恢复和生态建设具有重要意义。采用基于FAO Penman-Monteith的降水蒸散比来描述区域的干湿状况, 划分了黄河上游地区的干湿气候区。在此基础上, 利用AVHRR归一化植被指数(NDVI)和GLOPEM净初级生产力(NPP)数据集和同期的气候资料, 分析了黄河上游植被覆盖、植被生产力和气候变化的趋势, 探讨了不同干湿气候区影响植被变化的主要气候因子。结果表明, 研究区域东南部为半湿润气候区, 其余为半干旱气候区, 干湿气候分界线与450 mm降水等值线较接近; 1981–2006年区域气候趋于干暖化, 尤其是气温的升高趋势明显; 半湿润地区NDVI和NPP显著增加, 半干旱地区略有增加; 半湿润地区的NDVI多与气温显著正相关, 与降水量的相关性较弱, 气温是植被生长的主要气候制约因素; 半干旱地区的NDVI则与降水量的正相关性更强, 对降水量的变化较为敏感。NPP对气候变化的响应模式与NDVI相似。植被对气候变化的响应部分依赖于研究区域所具备的水热条件, 干湿气候划分有助于更好地解释植被对气候变化响应的空间差异。     

秦巴山地NPP及对气候变化响应的多维地带性与暖温带-亚热带界线

秦巴山地位于我国的南北过渡带,对我国生态地理格局产生重要影响。为了探索秦巴山地植被净第一性生产力(NPP,Net Primary Productivity)的时空格局及其气候响应的多样性和复杂性,为我国暖温带-亚热带界线的具体分布提供新的佐证,基于2000—2015年的MOD17A3的地表植被NPP数据和秦巴山地93个气象站点数据,从经度、纬度、海拔、坡向多个维度研究了秦巴山地地表植被NPP的分布及与气候因子的关系。结果表明:从2000—2015年,(1)秦巴山地中低山地区,自北向南随纬度降低,地表植被多年平均NPP呈现增加的趋势,体现了纬度地带性;年均NPP与温度的关系由负相关变为正相关,转折点出现在汉江;与降水的相关性减弱。(2)自西向东多年平均NPP值先增加后减少,秦岭一线地表植被年均NPP与温度由正相关变为负相关,与降水主要呈正相关,相关性先增加后减少。(3)随高度的增加,秦巴山地多年NPP值及增长率均呈现先增加后减少的趋势。(4)秦岭和大巴山多年平均NPP均呈现增加趋势,但是秦岭增长较大巴山更明显;2000m以下,秦岭南坡增长率明显高于北坡,大巴山北坡增长率明显高于南坡;2000—3000m,秦岭南北坡差异较小,但是大巴山差异明显;中山地区(1000—2500m),秦岭年均NPP与气温呈负相关,而大巴山则呈现正相关或弱相关;秦岭地区年均NPP与降水的相关性整体强于大巴山地区。这就意味着全球变暖、气温升高对秦岭植被尤其是中低山地区的植被产生不利影响,但是对大巴山则有利,而前者植被生长主要与降水增加有关。这也说明了基于汉江为界的秦岭和大巴山无论是地表植被NPP的均值还是其南北坡差异以及对气候因子的响应呈现了明显的差异,而汉江作为中山地区植被NPP与气温相关性由正相关性到负相关的转折点,与降水的关系由弱相关到正相关的转折点,更合适作为南北分界线。     

2001—2020年川西北高原归一化植被指数演变特征及其对极端气候的响应

川西北高原是典型的生态气候敏感区,其植被状况与气候变化密切相关。本研究基于2001—2020年MODIS-NDVI数据集和气象数据,采用最大值合成、地理探测器模型、线性趋势分析、相关分析等方法,研究川西北高原生长季归一化植被指数(NDVI)的变化趋势及其对气候因子的响应机制。结果表明: 研究期间,川西北高原植被覆盖度整体状况良好,86.8%的区域植被稳定,12.6%的区域NDVI呈弱持续性上升趋势,0.6%的区域NDVI呈下降趋势,全区生态环境呈稳中向好的发展趋势。研究区植被覆盖度空间差异大,总体呈由西南向东北上升的趋势,并有显著的立体变化。海拔1350 m以下,NDVI随海拔升高而上升;海拔1350～3650 m,NDVI无显著变化;海拔3650～5900 m,NDVI随海拔升高而下降,在4750～5900 m快速下降;海拔5900 m以上,几乎无植被。川西北高原的NDVI受多种自然因子交互作用影响,热量因子(月最高气温极大值、月最低气温极小值、植物生长期、年均温、生长期均温)是主导气候因子,除月最高气温极大值外,其余温度因子对NDVI均以正贡献为主。NDVI对气温指数的响应高于降水指数。在气候变暖背景下,极端气温暖指数对川西北高原植被生长尤其是高海拔地区植被生长及改善以促进作用为主。     

川渝地区NDVI动态特征及其对气候变化的响应

四川、重庆位于我国西南、长江上游地区,地形条件复杂,区内生态环境对气候变化较为敏感,作为我国现代农业发展区和长江中上游重要生态屏障,探究区域植被覆盖与气候变化之间的关系对该地区生态文明建设与农业可持续发展具有重要意义。基于归一化差值植被指数（NDVI）和气象数据（气温、降水）,采用Sen趋势分析、MK检验和偏相关分析法,以农业地貌分区视角分析1999-2018年川渝地区植被覆盖的时空变化特征,以及植被NDVI与气温和降水之间的时空响应。研究结果表明：（1）川渝地区及各农业地貌分区植被NDVI在近20年均呈显著上升趋势,增长速率为盆周山地区（0.0073/a）>四川盆地区（0.0063/a）>川西南山地区（0.0050/a）>川西高山高原区（0.0026/a）,整体年际变化率为0.0047/a。（2）川渝地区91.51%的NDVI像元值变化为正,空间上呈现出东高西低的总体分布格局,其中盆周山地区NDVI值最高为0.60,川西高山高原区NDVI值最低为0.44。（3）川渝地区整体上NDVI与气温和降水呈正相关,且气温（r=0.707,P<0.01）强于降水（r=0.535,P<0.05）,空间上植被NDVI与气温和降水呈显著正相关区域分别占39.31%、18.92%。（4）不同农业地貌区植被生长与气候变化的响应关系呈现出明显差异。在川西高山高原区NDVI与气温呈显著的正相关关系,但与降水的关系不明显,其中阿坝-若尔盖-红原等高平原地区及西北部石渠等丘状高原地区受气温的显著正效应驱动尤为明显;在四川盆地区和盆周山地区NDVI与气温和降水均呈正相关,特别是东北部平行岭谷及低山丘陵区受气温和降水的正效应影响显著。研究有助于进一步理解川渝地区植被与气候变化的响应机制,并为促进川渝地区生态建设及发展提供参考依据。     

乌伊岭国家级自然保护区植被覆盖演变及其对气候突变的响应

探究中国北方高纬度森林覆盖区植被演变受到气候因子变化乃至突变的影响,选用MODIS-NDVI数据与TM/ETM+数据,结合62年的气象观测数据,运用像元二分法模型、累计距平分析、Mann-Kendall非参数检验、滑动T检验与相关性分析,探讨了乌伊岭国家级自然保护区1975—2016年气候变化及其突变对植被覆盖的演化规律,并对不同气候因子与植被覆盖类型的空间变化进行相关性分析。结果表明:(1)乌伊岭保护区气候变化呈现暖干化发展的趋势。年均气温上升(0.557℃/10a),年均最低气温与冬季增温幅度最快,秋季最慢。降水量年际变化较小(-14.052 mm/10a),季节性变化明显。经突变性检验,1980—1995年是气候增温减湿的突变时期。(2)研究区植被生长季的NDVI为0.673,有植被覆盖的区域占87.69%,其中高等植被覆盖区所占比最大。(3)气候突变时期,生长季NDVI显著下降,植被退化严重。低植被覆盖区无显著变化,而高植被覆盖区开始逐渐退化为中等与中低等植被覆盖区。在空间上植被覆盖的退化状况主要由中心山地沿四周低山丘陵区累年逐渐降低。不同植被覆盖区域下降的幅度:混交林草地针叶林耕地湿地。(4)乌伊岭保护区年均最大NDVI与年均气温和年均降水量的相关系数分别为0.261、0.068,其中呈正相关区域占总面积56.67%和42.79%,在分布趋势上两者都表现出明显的空间差异性。而气温因子影响植被覆盖的空间范围与能力更强,空间相关性更高,也是影响植被退化的主导因素。     

Stomach cancer incidence trends in selected Latin America countries: Age,period, and birth-cohort effects

Backgroundto explore the age, period, and birth-cohort effects on stomach cancer incidence trends during 3 decades in selected Latin American countries.Methodsa time-trend study was performed using Cancer Incidence in Five Continents data from high-quality population-based cancer registries(PBCRs) in Latin American countries. Crude and age-standardized incidence rates(ASRIs) were calculated. Time trends in ASRIs were assessed using the average annual percentage change(AAPC). Age-period-cohort effects were estimated by Poisson regression for individuals aged between 20 and 79 years with stomach cancer informed by PBCRs from 1983 to 2012 in Cali(Colombia); from 1982 to 2011 in Costa Rica; and from 1988 to 2012 for Goiania(Brazil) and Quito(Ecuador). The goodness-of-fit model was tested using the deviance of the models.Resultsa decrease in age-standardized incidence rates was observed for both genders in all populations covered by PBCRs, except for young men from Cali(AAPC 3.89 95 %IC: 1.32–7.29). The age effect was statistically significant in all areas, and the curve slope reached peaks in the older age groups. The cohort effect was observed in all PBCRs. Regarding the period effect, an increased ratio rate was observed for both genders in Costa Rica(1997–2001 women RR 1.11 95 %CI: 1.05–1.17; men RR 1.12 95 %CI: 1.08–1.17) and Goiânia(2003–2007 women RR 1.21 95 %CI: 1.08–1.35; men RR 1.09 95 %CI: 1.01–1.20), while Quito(1998–2002 women RR 0.89 95 %CI: 0.81–0.98; men RR 0.86 95 %CI: 0.79–0.93) presented a decrease.Conclusionthe present study showed a decreasing gastric cancer trend for over the past 30 years with gender and geographic variations. Such a decrease seems to be mainly a result of cohort effects, suggesting that the economic market opening process led to changes in the risk factor exposures over successive generations. These geographic and gender variations may reflect cultural/ethnic/gender differences and differences in dietary and smoking rate patterns. However, an increased incidence was observed for young men in Cali, and additional studies are needed to determine the cause of the increasing incidence in this group.     

Dynamic analysis of recurrent event data using the additive hazard model

We propose a method for analysis of recurrent event data using information on previous occurrences of the event as a time-dependent covariate. The focus is on understanding how to analyze the effect of such a dynamic covariate while at the same time ensuring that the effects of treatment and other fixed covariates are unbiasedly estimated. By applying an additive regression model for the intensity of the recurrent events, concepts like direct, indirect and total effects of the fixed covariates may be defined in an analogous way as for traditional path analysis. Theoretical considerations as well as simulations are presented, and a data set on recurrent bladder tumors is used to illustrate the methodology.     

Transgenerational effects benefit offspring across diverse environments: a meta‐analysis in plants and animals

The adaptive value of transgenerational effects (the ancestor environmental effects on offspring) in changing environments has received much attention in recent years, but the related empirical evidence remains equivocal. Here, we conducted a meta‐analysis summarising 139 experimental studies in plants and animals with 1170 effect sizes to investigate the generality of transgenerational effects across taxa, traits, and environmental contexts. It was found that transgenerational effects generally enhanced offspring performance in response to both stressful and benign conditions. The strongest effects are in annual plants and invertebrates, whereas vertebrates appear to benefit mostly under benign conditions, and perennial plants show hardly any transgenerational responses at all. These differences among taxonomic/life‐history groups possibly reflect that vertebrates can avoid stressful conditions through their mobility, and longer‐lived plants have alternative strategies. In addition to environmental contexts and taxonomic/life‐history groups, transgenerational effects also varied among traits and developmental stages of ancestors and offspring, but the effects were similarly strong across three generations of offspring. By way of a more comprehensive data set and a different effect size, our results differ from those of a recent meta‐analysis, suggesting that transgenerational effects are widespread, strong and persistent and can substantially impact the responses of plants and animals to changing environments.     

Craniometrical variation among South American prehistoric populations: climatic, altitudinal, chronological, and geographic contributions

The independent contributions of climate, altitude, chronology, and geographic location of archeological sites to craniometrical variation are analyzed in a sample of 1,119 skulls from South America. Geographic location is responsible for the highest proportion of craniometrical variation, followed by climate and altitude. It is concluded that geographic isolation has partially prevented gene flow from counterbalancing craniometrical microdifferentiation produced by founder effect.     

Experimental and theoretical DFT (B3LYP,X3LYP,CAM-B3LYP and M06-2X) study on electronic structure,spectral features,hydrogen bonding and solvent effects of 4-methylthiadiazole-5-carboxylic acid

Detailed structural, electronic and spectroscopic study of 4-methylthiadiazole-5-carboxylic acid, one of the simplest 1,2,3-thiadiazole derivatives has been performed using density functional theory at four different functionals (B3LYP, X3LYP, CAM-B3LYP and M06-2X). The two possible conformers and their dimeric forms have been investigated for the stability and hence for the calculation of molecular properties of the title compound. Vibrational analysis has been performed with the help of experimental FT-IR and FT-Raman spectra. NBO analysis has been performed to estimate the N–H—O=C hydrogen bond strength and to evaluate the intra and inter molecular charge transfer in the system. Intermolecular hydrogen-bond strength has also been computed using Atoms in Molecules (AIM) theory. To visualise spatial domain, key sites of electron transitions and electron density difference between ground as well as excited states, and their 2D and 3D plots have been computed. Solvent effect on the intermolecular hydrogen bonding have also been investigated using solvents of different polarities. Non-linear optical properties, molecular electrostatic potential surface map (MESP), thermodynamic potentials at different temperatures have also been computed and plotted.     

The Genus Uroleucon Mordvilko (Insecta, Aphidoidea) in South America, with a key and descriptions of four new species

Uroleucon is one of only two genera of Aphidinae to have undergone significant speciation in South America, with a group of about 14 endemic, morphologically similar species feeding on native Compositae, especially Baccharis . Multivariate morphometrics (canonical variates analysis, CVA) was used to discriminate between these species and compare them with the North American subgenus Lambersius , in which most of them have hitherto been placed. It is concluded that they probably form a separate monophyletic group. Four new species are described in this group— brevisiphon, essigi, petrohuense and pseudomuennosum— and five names are placed in synonymy. Of the other species of Uroleucon in South America, U. lizerianum is widespread and feeds on many species of native and introduced Compositae; it is morphometrically inseparable from the North American species U. ambrosiae and is probably an introduced form of that species, but has consistently fewer secondary rhinaria. A key is provided to the 19 available species of Uroleucon recorded from South America.     

多尺度上的多物种多格局——以莫索湾沙漠四种灌木及其生境为例

植物的生长固然与自身特性有关,也与其生境中环境要素及其它物种相互作用相互影响,牵涉到生物格局、环境格局和景观格局的相互作用,已有研究表明,这种作用具有多尺度特性.量化这3种格局的某些重要特征,将有利于理解这种尺度行为对格局的影响,甚至量化格局在多尺度上的一些特性.在古尔班通古特沙漠南缘莫索湾沙地选取相隔15km的两个200m×200m样地,以心叶驼绒藜(Ceratoides ewersmanniana)、梭梭(Haloxylon ammodendron)、琵琶柴(Perosimonia soongorica)和沙拐枣(Calligonum mongolicum)及其生境地形和土壤理化性质为研究对象,联合小波分析(WAA)、点格局分析(PPA)和典范对应分析(CCA)研究莫索湾沙漠4种灌木种内的聚集性(成丛性)及其周期性、种间相互作用、边缘效应及其与生境地形和土壤理化性质的关系,发现表观上沙生植物分布非连续,但是可形成25～80m尺度范围的成丛性分布,且周期性出现,心叶驼绒藜周期为115～125m、梭梭周期为130～140m,研究表明,周期为110m的地形基频是植物成丛性周期形成的主因.琵琶柴和沙拐枣虽成丛分布,但未发现其周期,其周期可能在更大的幅度上存在;其次,东西方向的边缘量化检测结果表明:东西方向也存在功能边缘和结构边缘,不像南北边缘的位置是受到太阳辐射和遮阴作用影响,东西边缘的位置却是受到了垄状沙丘大小和地形的影响;再次,种间关系研究发现:4种灌木相互都是负相关,且在大多数尺度上是显著负相关.结果表明:荒漠植物通过成丛或聚集分布可以形成适合本物种生存的局部微生境.     

Testing for the influence of niche and neutral factors on sapling community assembly beneath isolated woody plants in grasslands

Questions: Perching and nursing effects drive initial steps of forest expansion over grasslands. Nursing effect is obviously related to niche mechanisms, while perching effect is likely to result both from neutral and niche factors. This study assessed the effect of neutral and niche factors on species composition in sapling communities developing beneath isolated trees/shrubs (ITS) in grassland. Location: A mosaic of Campos grassland and Araucaria forest in São Francisco de Paula, southern Brazil (29°28′S, 50°13′W). Methods: We described sapling communities beneath 32 ITS using mean number of forest woody saplings of different species. We performed a stepwise canonical correspondence analysis (CCA) to select ITS traits that maximized the association with species composition. Then we evaluated the contribution of distance from seed source, ITS traits and distance‐structured ITS traits on sapling community assembly using a variation partitioning method based on CCA and partial CCA. Results: Sapling species composition was significantly explained by ITS traits (ITS dispersal mode, ITS growth form, crown area:ITS height ratio, crown area, ITS height and crown area:volume ratio). Distance from seed sources explained only a minor, non‐significant fraction of sapling species composition. Distance‐structured trait variation was negligible. Conclusions: Sapling community assembly beneath ITS was mostly explained by niche factors related to both nursing and perching effects. Dispersal limitation explained only a small fraction of variation in species composition beneath ITS, suggesting that neutral‐based perching effect had a minor role in community assembly.     

四种草原植物根际土壤线虫群落特征

植物是否能特异性地影响土壤生物群落逐渐受到生态学家的关注。本研究利用盆栽实验研究了内蒙古典型草原区3种建群种(大针茅、克氏针茅和羊草)和1种群落伴生种(冰草)根际土壤线虫群落的数量特征。结果表明:4种植物根际土壤线虫群落间差异显著,这不仅表现在线虫密度方面,也表现在线虫种属组成、营养类群比例、优势类群以及群落结构方面,其中冰草根际土壤线虫群落的成熟度指数最低;判别分析能够将同一种植物根际土壤线虫群落样品与其他种植物根际线虫群落样品很好地分开;聚类分析显示,大针茅和克氏针茅根际线虫群落首先聚在一起,后与冰草根际线虫群落聚在一起,最后与羊草根际线虫群落聚在一起,与这4种植物的系统关系一致。     

Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry

Matrix effects caused by compounds endogenous to the biological sample are a primary challenge in quantitative LC/MS/MS bioanalysis. Many approaches have been developed to minimize matrix effects such as optimization of sample extraction procedures and use of isotopically labeled internal standards. Unexpected matrix components may still remain undetected, however, because of the selective mass transitions monitored during MS/MS analysis. Glycerophosphocholines are the major phospholipids in plasma that have been widely shown to cause significant matrix effects on electrospray ionization efficiencies for target analytes. The purpose of this work was to investigate potential matrix effects resulting from different endogenous lipid classes, including phospholipids, acylglycerols and cholesterols, in order to establish a library for the relative presence of these components in biological sample extracts obtained by commonly used sample preparation techniques. Thirteen compounds were selected which were representatives of eight phospholipids classes, mono, di, triacylglycerols, cholesterol and cholesterol esters. Post-column infusion experiments were carried out to compare relative ion suppression effects of these compounds. Chlorpheniramine and loratadine were selected as model test analytes. A Concentration Normalized Suppression Factor (%CNSF) was defined to allow comparison of ion suppression effects resulting from different endogenous lipids according to their typical concentrations in human plasma and erythrocytes. A simple LC/MS/MS method was developed to monitor these endogenous components in sample extracts and their extraction recoveries from a plasma pool were compared using protein precipitation, liquid-liquid extraction, supported-liquid extraction, solid phase extraction and Hybrid SPE-precipitation methods. Endogenous lipid components other than GPChos, such as cholesterols and triacylglycerols, may result in significant matrix effects and should be monitored during method development. No single extraction procedure was efficient in removing all of the various lipid components. Use of the results presented here, along with a consideration of analyte chemical structure, the type of matrix and the type of sample preparation procedure, may help a bioanalytical scientist to better anticipate and minimize matrix effects in developing LC/MS/MS-based methods.