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1.
Dong-Yuan Zhang Na Chen Yan-Zhuo Yang Qian Zhang Jian-Quan Liu 《Conservation Genetics》2008,9(2):475-477
Rhubarb is an important Traditional Chinese Medicine. However, the wild resource has been declining. In order to design appropriate
conservation methods for the official species across their natural distributions, it is important to characterize their genetic
diversity. Here, we describe the development of 10 new microsatellite loci for AC/TG/CCA in Rheum tanguticum. The microsatellites were enriched using the combined biotin capture method. The polymorphism of each locus was further assessed
in 12 individuals from four geographically distinct populations of this species. The number of alleles ranged from three to
seven and the expected heterozygosity ranged from 0.53 to 0.73. All markers have been checked in the other three species in
the genus and two of them together comprise the official medicinal rhubarb resource with R. tanguticum. These microsatellite markers could provide a useful tool for genetic and conservation studies of the rhubarb species. 相似文献
2.
Inmaculada López-Flores Víctor N. Suárez-Santiago Ana T. Romero-García Roberto de la Herrán Gabriel Blanca 《Conservation Genetics》2008,9(6):1695-1697
We report on the isolation and characterization of eight microsatellite markers from enriched libraries for Arenaria nevadensis, one of the most critically endangered plant species in the Iberian Peninsula. These are the first microsatellite loci reported
for Arenaria species. The number of alleles ranged from two to eight, and the expected heterozygosity from 0.067 to 0.873. These markers
will be useful for characterizing the genetic diversity in A. nevadensis and understanding its population structure, and will provide important genetic data for the conservation and recovery of
this species. 相似文献
3.
Codonopsis pilosula Nannf., as an important traditional Chinese medicinal plant species, has been used to treat fatigue, thirst and loss of appetite.
In this study, we developed 10 new microsatellite loci primers from the genome of this species using the combined biotin capture
method. The polymorphism of each locus was further assessed through 27 individuals from four geographically distant populations.
The number of alleles per locus ranged from 6 to 11. The observed and expected heterozygosity ranged from 0.15 to 0.24 and
0.27 to 0.40, respectively. We further performed cross-priming tests of these loci in the other three congeneric species.
These microsatellite markers will be useful for investigating genetic diversity within and between populations of these species. 相似文献
4.
Genetiana crassicaulis is one of famous Chinese medicinal plant. The over-collection for its root has caused its dramatic reduction. In order to
devise adequate conservation and management strategies for this species, it is important to characterize its genetic diversity
and understand its population structure. Here, 10 polymorphic microsatellite markers have been developed. AC/TG microsatellite
was enriched by combining biotin capture method. Polymorphism of each locus was assessed in 30 individuals from six populations.
The number of alleles ranged from 2 to 9 and the expected heterozygosity ranged from 0.32 to 0.78. 相似文献
5.
Peganum harmala is a herb grows spontaneously in arid and rocky areas. From ancient time, it has been claimed to be an important medicinal
plant in rough environment. In this study, we developed 12 microsatellite loci from P. harmala by the combining biotin capture method for the first time. A total of 31 microsatellite sequences were recovered through
screening the library and 12 of them are polymorphic. The number of alleles per locus in 36 sampled individuals ranged from
3 to 8, expected heterozygosity and observed heterozygosity ranged from 0.1381 to 0.6821 and from 0.3573 to 0.8739, respectively.
In addition, all markers have been crossly checked in the other congeneric species. These microsatellite markers would provide
a useful tool for investigating the genetic diversity and study the population genetic structure in detail. 相似文献
6.
薄叶金花茶、小花金花茶和小瓣金花茶是三种濒危金花茶植物,为了解珍稀濒危植物遗传多样性和遗传结构,该研究利用微卫星标记对他们的7个种群共184个个体进行了遗传多样性和遗传结构分析。结果表明:11个位点共检测到等位基因92个。在物种水平上,小瓣金花茶平均等位基因数(N_A)为3.9、有效等位基因数(N_E)为2.328、观测杂合度(H_o)为0.520、期望杂合度(H_e)为0.501,高于薄叶金花茶和小花金花茶。在种群水平上,有效等位基因数(N_E)在1.788~2.466之间,期望杂合度(H_e)在0.379~0.543之间;种群间遗传分化系数(FST)在0.143 7~0.453 3之间,种群间基因流(N_m)在0.301 5~1.488 9之间。AMOVA分子变异分析显示65.72%的变异存在于种群内。三种金花茶具有较低水平的遗传多样性和高水平的种群间遗传分化。STRUCTURE和PCoA种群遗传结构分析结果将取样种群分为2组,即薄叶金花茶和小花金花茶大部分个体分为一组,小瓣金花茶大部分个体分为一组。现存所有种群应根据实际情况尽快采取就地保护或迁地保护措施。 相似文献
7.
Eleven dinucleotide and five tetranucleotide polymorphic microsatellite loci were presented for the Indo-Pacific humpback
dolphin Sousa chinensis, a species categorized as ‘data deficiency’ in the IUCN Red List. These markers were developed to allow future population
studies, such as characterization of population structure and genetic diversity that are important for the species’ conservation.
The number of alleles ranged from 2 to 9 with observed heterozygosity ranging from 0.167 to 0.917, and expected heterozygosity
ranging from 0.159 to 0.913. Test of these loci in five additional cetacean species found that 10–13 loci have successful
cross-amplifications. 相似文献
8.
Sinadoxa corydalifolia is the only species of Sinadoxa (Adoxaceae) with the aberrant morphology. This species has become extremely endangered in the Qinghai-Tibetan Plateau. To
provide a population-level genetic profile for investigation and conservation of genetic diversity of this species, we developed
10 new microsatellite loci for this species by the combining biotin capture method. About 31 microsatellites were screened
from the library, 10 of the screened microsatellites are polymorphic. The number of alleles per locus in 18 individuals ranged
from 3 to 11, expected heterozygosity and observed heterozygosity ranged from 0.3071 to 0.6243 and from 0.1675 to 0.4357,
respectively. We further performed cross-priming tests of these primers in another species of the Adoxaceae: Adoxa moschatellina and found 9 of 10 successfully amplified the targeted sequences. These newly developed loci provide a useful tool to investigate
the genetic diversity and design the conversation measures of S. corydalifolia and study the genetic divergence and the initial speciation pattern between it and the related species in the Adoxaceae. 相似文献
9.
TINA KYNDT ANNELIES HAEGEMAN SABINE VAN GLABEKE INGE MAERTENS BART VAN DROOGENBROECK ISABEL ROLDN‐RUIZ GODELIEVE GHEYSEN 《Molecular ecology resources》2005,5(3):590-592
Nine polymorphic microsatellite loci were identified in the tropical plant Vasconcellea ×heilbornii and used to estimate allelic diversity in two populations of southern Ecuador. Allelic richness ranged from two to five alleles, observed heterozygosity ranged from 0.150 to 0.947 and expected heterozygosity ranged from 0.186 to 0.701. Most of these markers also amplified microsatellite loci in two other Vasconcellea species (Vasconcellea stipulata and Vasconcellea cundinamarcensis). Hence, these markers will be useful for population genetic analysis and the evaluation of genetic diversity and gene flow in these species. 相似文献
10.
Dahai Gao Qian Wang Yuxia Wu Haiyan Xu Qiushi Yu Jianquan Liu 《Conservation Genetics》2008,9(4):953-955
Thellungiella
salsuginea (Brassiaceae) is a typical halophyte which can tolerate extreme cold, drought, and salinity. In order to understand the adaptive
evolution of this species in the arid habitats, it is important to know its genetic structure. In this study, 17 polymorphic
microsatellite loci were isolated and characterized from an enrichment genomic library of this species. We further assessed
the polymorphisms of each locus in 18 individuals from nine geographically distant populations. The number of alleles per
locus ranged from six to fourteen. The observed and expected heterozygosity ranged from 0.17 to 0.28 and 0.32 to 0.45, respectively.
These markers have been crossly checked in another congeneric species, T. halophila. These microsatellite markers will be useful for investigating population genetics and adaptive evolution of this species
and morphological divergence between and it and the closely related species. 相似文献
11.
The razor clam (Sinonovacula constricta) is an important aquacultured bivalve in China. The natural populations of this species are decreasing quickly. To facilitate
studies on genetic diversity and population structure of wild populations, microsatellites were isolated from a CA enriched
genomic library. Eight microsatellite loci were polymorphic in 30 individuals from Chongming in Shanghai, China. The number
of alleles per polymorphic locus varied from 6 to 13 and the values of observed heterozygosity and expected heterozygosity
ranged from 0.350 to 1.000 and from 0.602 to 0.902, respectively. These microsatellites are being used in studying population
differentiation and genetic diversity for effective conservation and management genetic resources of S. constricta. 相似文献
12.
Chinese alligator (Alligator sinensis) is a critically endangered species endemic to China. In this study, the extent of genetic variation in the captive alligators
of the Changxing Reserve Center was investigated using microsatellite markers derived from American alligators. Out of 22
loci employed, 21 were successfully amplified in the Chinese alligator. Sequence analysis showed loci in American alligators
had a bigger average size than that of the Chinese alligators and the longest allele of an individual locus almost always
existed in the species with longer stretch of repeat units. Eight of the 22 loci were found to be polymorphic with a total
of 26 alleles present among 32 animals scored, yielding an average of 3.25 alleles per polymorphic locus. The expected heterozygosity
(H
E) ranged at a moderate level from 0.4385 to 0.7163 in this population. Compared to that in the American alligators, a lower
level of microsatellite diversity existed in the Changxing population as revealed by about 46% fewer alleles per locus and
smaller H
E at the homologous loci. The average exclusion power and the ability to detect shared genotypes and multiple paternity were
evaluated for those markers. Results suggested that when the polymorphic loci were combined, they could be sensitive markers
in genetic diversity study and relatedness inference within the Chinese alligator populations. The level of genetic diversity
present in the current Changxing population indicated an important resource to complement reintroductions based on the individuals
from the other population. In addition, the microsatellite markers and their associated diversity characterized in this population
could be utilized to further investigate the genetic status of this species. 相似文献
13.
Qian Zhang Yan-Zhuo Yang Gui-Li Wu Dong-Yuan Zhang Jian-Quan Liu 《Conservation Genetics》2008,9(3):767-769
Juniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important
role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined.
In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within
and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin
capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals
from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity
ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species.
In addition, all markers have been crossly checked in the other four congeneric species. 相似文献
14.
Hung-Du Lin Teh-Wang Lee Fang-Jiao Lin Chung-Jian Lin Tzen-Yuh Chiang 《Conservation Genetics》2008,9(4):945-947
Pararasbora moltrechti (Cyprinidae) is an endangered freshwater fish with a scattered distribution in the central Taiwan. In order to undertake
a conservation program for this species in Taiwan, it is essential to evaluate its genetic diversity and population structure.
For this purpose, we described the development of eight microsatellite loci in P. moltrechti for genetic studies. These new molecular markers were tested in 36 individuals of the rare species. The number of alleles
per locus ranged from 8 to 14. As the expected heterozygosity varied from 0.770 to 0.932, all microsatellite loci, however,
were significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency.
Hung-Du Lin and Teh-Wang Lee contributed equally. 相似文献
15.
Eleven polymorphic microsatellite loci were isolated and characterized from an AC-enriched genomic library of Akebia trifoliate ssp. australis. The number of allele per locus ranged from 3 to 14. The observed heterozygosity and expected heterozygosity at population
level were 0.196–1.000 and 0.522–0.902, respectively. In addition, this set of microsatellites produced robust cross-species
amplification in other two related taxa, suggesting these microsatellite markers should provide a useful tool for genetic
and conservation studies of the Akebia species. 相似文献
16.
Hong-Li Tian Xiao-Qing Chen Jian-Xiu Wang Jian-Hua Xue Jun Wen Grant Mitchell Shi-Liang Zhou 《Conservation Genetics》2008,9(5):1385-1388
This paper reports the development of microsatellite primers for Nelumbo nucifera Gaerten. By screening genomic libraries enriched with 10 kinds of probes, Seventeen polymorphic loci were isolated and primers
were designed. Polymorphism of these 17 loci was assessed in 24 individuals. All the 17 loci are polymorphic and the number
of alleles ranged from two to seven. Observed heterozygosity and expected heterozygosity ranged from 0.0000 to 0.9176 and
from 0.2837 to 0.7917 respectively. These microsatellite loci should be useful for studying the genetic diversity of N. nucifera. 相似文献
17.
Noble crayfish (Astacus astacus) is listed as vulnerable by the IUCN Red List of Threatened Species in Europe. However, very little is known about genetic
diversity and structuring of noble crayfish populations, mainly because of the lack of informative genetic markers. We describe
the isolation and characterization of the first microsatellite markers for this species, which were obtained by screening
4,000 recombinant clones. Eight loci revealed polymorphisms in a panel of 172 individuals from seven populations in Northern
Europe. Number of alleles per locus ranged from two to 10 (average 4.4) and heterozygosity levels among populations varied
from 0 to 0.80 for H
o and from 0 to 0.72 for H
e. 相似文献
18.
We have isolated and characterized twelve novel polymorphic microsatellite loci from Scapharca subcrenata to analyse the population structure. The number of observed alleles per locus ranged from 3 to 17. Observed heterozygosity
(H
O) ranged from 0.321 to 0.929. Cross-species amplification was tested successfully in three other bivalve species. These microsatellite
markers will be useful for genetic diversity studies of S. subcrenata and other Lamellibranchia species. 相似文献
19.
Ramesh K. Aggarwal Albert Lalremruata Thirumalaisamy P. Velavan Ayyadevara Pavani Sowjanya Lalji Singh 《Conservation Genetics》2008,9(4):981-984
Olive ridleys, one of the widely distributed marine turtle species has undergone declines in recent years due to multiple
anthropogenic factors warranting conservation efforts for which assessment of genetic variability in existing populations
become critical. Here we describe development of ten new microsatellite markers from a short sequence repeat-enriched partial
genomic DNA library, which are found to be highly informative for genetic studies. Eight of these markers when tested on 83
olive ridley turtles revealed high allelic diversity (4–27 alleles per marker), and high observed and expected heterozygosity
estimates that ranged from 0.29 to 0.82 and 0.62 to 0.94, respectively. Two microsatellites were monomorphic in the tested
olive ridley samples, but were found to be informative/polymorphic when tested on related marine turtle species. More importantly,
nine of the new markers showed robust cross-species amplifications in three related species Dermochelys coriacea, Chelonia mydas and Eretmochelys imbricata. Thus, this study describes ten new microsatellite markers and also demonstrates their potential as efficient genetic markers
in studies related to parentage analysis, population structure, phylogeography and species relationships of olive ridleys
and other marine turtle species. 相似文献
20.
GUANGXIANG TONG YOUYI KUANG JIASHENG YIN LIQUN LIANG XIAOWEN SUN 《Molecular ecology resources》2006,6(4):1099-1101
Hucho taimen (Pallas) is an endangered species in China. To evaluate the species’ genetic diversity and population genetic structure, we isolated six polymorphic microsatellite markers from its genomic libraries by (ACA)16 enriched, and characterized using 61 wild individuals. The number of alleles per locus ranged from two to seven, expected heterozygosity ranged from 0.3200 to 0.7410, polymorphism information content ranged from 0.3047 to 0.6896. These markers will be useful for the genetic variation assessment of taimen. 相似文献