共查询到20条相似文献,搜索用时 15 毫秒
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Insulator elements can be classified as enhancer-blocking or barrier insulators depending on whether they interfere with enhancer-promoter interactions or act as barriers against the spreading of heterochromatin. The former class may exert its function at least in part by attaching the chromatin fiber to a nuclear substrate such as the nuclear matrix, resulting in the formation of chromatin loops. The latter class functions by recruiting histone-modifying enzymes, although some barrier insulators have also been shown to create chromatin loops. These loops may correspond to functional nuclear domains containing clusters of co-expressed genes. Thus, insulators may determine specific patterns of nuclear organization that are important in establishing specific programs of gene expression during cell differentiation and development. 相似文献
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A hypothesis for chromatin domain opening 总被引:2,自引:0,他引:2
Xin L Liu DP Ling CC 《BioEssays : news and reviews in molecular, cellular and developmental biology》2003,25(5):507-514
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The control of gene expression involves regulatory elements that can be very far from the genes they control. Several recent technological advances have allowed the direct detection of chromatin loops that juxtapose distant genomic sites in the nucleus. Here we review recent studies from various model organisms that have provided new insights into the functions of chromatin loops and the mechanisms that form them. We discuss the widespread impact of chromatin loops on gene activation, repression, genomic imprinting and the function of enhancers and insulators. 相似文献
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Genomic insulators: connecting properties to mechanism 总被引:25,自引:0,他引:25
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Human tRNA genes function as chromatin insulators 总被引:1,自引:0,他引:1
Raab JR Chiu J Zhu J Katzman S Kurukuti S Wade PA Haussler D Kamakaka RT 《The EMBO journal》2012,31(2):330-350
Insulators help separate active chromatin domains from silenced ones. In yeast, gene promoters act as insulators to block the spread of Sir and HP1 mediated silencing while in metazoans most insulators are multipartite autonomous entities. tDNAs are repetitive sequences dispersed throughout the human genome and we now show that some of these tDNAs can function as insulators in human cells. Using computational methods, we identified putative human tDNA insulators. Using silencer blocking, transgene protection and repressor blocking assays we show that some of these tDNA-containing fragments can function as barrier insulators in human cells. We find that these elements also have the ability to block enhancers from activating RNA pol II transcribed promoters. Characterization of a putative tDNA insulator in human cells reveals that the site possesses chromatin signatures similar to those observed at other better-characterized eukaryotic insulators. Enhanced 4C analysis demonstrates that the tDNA insulator makes long-range chromatin contacts with other tDNAs and ETC sites but not with intervening or flanking RNA pol II transcribed genes. 相似文献
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Insulators are regulatory DNA elements restricting gene activation by enhancers. Interactions between insulators can lead
to both insulation and activation of promoters by enhancers. In this work, we analyzed the effects of interaction of two Drosophila insulators, Wari and Su(Hw). The functional interaction between these insulators was found to enhance the activity of the
Su(Hw) insulator only, but not of the Wari insulator. This suggests that the formation of a chromatin loop between interacting
insulators is not a key factor for enhancement of insulation, which is in disagreement with the main idea of structural models.
In addition, the effect of interaction between Wari and Su(Hw) depends on a distance between them and on the position in the
system relative to other regulatory elements. 相似文献
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Iampietro C Cléard F Gyurkovics H Maeda RK Karch F 《Development (Cambridge, England)》2008,135(24):3983-3987
Although the boundary elements of the Drosophila Bithorax complex (BX-C) have properties similar to chromatin insulators, genetic substitution experiments have demonstrated that these elements do more than simply insulate adjacent cis-regulatory domains. Many BX-C boundaries lie between enhancers and their target promoter, and must modulate their activity to allow distal enhancers to communicate with their target promoter. Given this complex function, it is surprising that the numerous BX-C boundaries share little sequence identity. To determine the extent of the similarity between these elements, we tested whether different BX-C boundary elements can functionally substitute for one another. Using gene conversion, we exchanged the Fab-7 and Fab-8 boundaries within the BX-C. Although the Fab-8 boundary can only partially substitute for the Fab-7 boundary, we find that the Fab-7 boundary can almost completely replace the Fab-8 boundary. Our results suggest that although boundary elements are not completely interchangeable, there is a commonality to the mechanism by which boundaries function. This commonality allows different DNA-binding proteins to create functional boundaries. 相似文献
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PRE-mediated bypass of two Su(Hw) insulators targets PcG proteins to a downstream promoter 总被引:1,自引:0,他引:1
Comet I Savitskaya E Schuettengruber B Nègre N Lavrov S Parshikov A Juge F Gracheva E Georgiev P Cavalli G 《Developmental cell》2006,11(1):117-124
Drosophila Polycomb group response elements (PRE) silence neighboring genes, but silencing can be blocked by one copy of the Su(Hw) insulator element. We show here that Polycomb group (PcG) proteins can spread from a PRE in the flanking chromatin region and that PRE blocking depends on a physical barrier established by the insulator to PcG protein spreading. On the other hand, PRE-mediated silencing can bypass two Su(Hw) insulators to repress a downstream reporter gene. Strikingly, insulator bypass involves targeting of PcG proteins to the downstream promoter, while they are completely excluded from the intervening insulated domain. This shows that PRE-dependent silencing is compatible with looping of the PRE in order to bring PcG proteins in contact with the promoter and does not require the coating of the whole chromatin domain between PRE and promoter. 相似文献
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Nicolas Nègre Christopher D. Brown Parantu K. Shah Pouya Kheradpour Carolyn A. Morrison Jorja G. Henikoff Xin Feng Kami Ahmad Steven Russell Robert A. H. White Lincoln Stein Steven Henikoff Manolis Kellis Kevin P. White 《PLoS genetics》2010,6(1)
Insulators are DNA sequences that control the interactions among genomic regulatory elements and act as chromatin boundaries. A thorough understanding of their location and function is necessary to address the complexities of metazoan gene regulation. We studied by ChIP–chip the genome-wide binding sites of 6 insulator-associated proteins—dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF—to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined insulators. We find two major classes of insulators defined by dCTCF/CP190/BEAF-32 and Su(Hw), respectively. Distributional analyses of insulators revealed that particular sub-classes of insulator elements are excluded between cis-regulatory elements and their target promoters; divide differentially expressed, alternative, and divergent promoters; act as chromatin boundaries; are associated with chromosomal breakpoints among species; and are embedded within active chromatin domains. Together, these results provide a map demarcating the boundaries of gene regulatory units and a framework for understanding insulator function during the development and evolution of Drosophila. 相似文献
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DNA replication begins from multiple sites distributed throughout the genome, named replication origins. Despite the increasing amount of data on the properties of replication origins, it is still unknown what factors are the primary determinants of ORC localization. Su(Hw) is a zinc-finger protein responsible for the activity of the best-studied Drosophila insulators. In the present work, we show that the insulator protein Su(Hw) recruits the histon acetyltransferase complex SAGA and chromatin remodeler dSWI/SNF to Su(Hw)-dependent insulators and creates a platform for ORC binding. We have found Su(Hw) to be necessary for chromatin remodeling and ORC recruitment regardless of the surrounding chromatin type. Thus, the global chromatin state does not affect the molecular mechanism underlying ORC positioning in genome; it is rather the DNA-binding proteins that are the key determinants that create the proper chromatin structure for ORC binding. Su(Hw) is the first example of such protein. 相似文献
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